Dolichol-linked oligosaccharide and glycoprotein biosyntheses in glial cells in primary culture: development and enzymatic correlates.

Abstract:

:Primary cultures of cerebral glia derived from neonatal rat brain were utilized to determine 1) the developmental changes of dolichol-linked oligosaccharide and N-linked glycoprotein biosyntheses, 2) the enzymatic correlates of these developmental changes, and 3) the temporal relations between the biosyntheses of dolichol-oligosaccharide and N-linked glycoproteins and the biochemical expression of astrocytic and oligodendroglial differentiation. Marked, parallel developmental increases in the rates of incorporation of [3H]glucosamine into both dolichol-oligosaccharide and glycoprotein were observed, with maximal rates achieved after 9-10 days in culture and little change occurring over the next 10 days in culture. Concerning the enzymatic correlates, dolichol kinase exhibited a moderate developmental increase with a maximum at 5 days in culture, whereas the activities of the three critical enzymes that utilize dolichyl phosphate in the synthesis of the dolichol-linked oligosaccharide, i.e., N-acetylglucosaminylphosphotransferase (GlcNAc-1-P transferase), mannosylphosphoryldolichol (Man-P-Dol) synthase, and glucosylphosphoryldolichol (Glc-P-Dol) synthase, reached maxima after 6-9 days in culture. Both the activity of Man-P-Dol synthase in vitro and the rate of formation of its product, Man-P-Dol, in intact cells were shown to correlate closely with the rates of oligosaccharide and glycoprotein biosyntheses. An important regulatory role for Man-P-Dol synthase and its product, Man-P-Dol, was suggested further by the demonstration of a maturation-dependent activation by Man-P-Dol of GlcNAc-1-P transferase, the first committed step in the pathway. Two enzymatic markers of astrocytic (glutamine synthetase) and oligodendroglial (2',3'-cyclic nucleotide 3'-phosphohydrolase) differentiation exhibited marked developmental increases in activity with onset at the time of attainment of peak rates of dolichol-oligosaccharide and glycoprotein biosyntheses. Importance of the latter processes for glial differentiation is suggested.

journal_name

J Neurosci Res

authors

Ishii S,Volpe JJ

doi

10.1002/jnr.490200409

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

463-72

issue

4

eissn

0360-4012

issn

1097-4547

journal_volume

20

pub_type

杂志文章
  • NADPH oxidase inhibitor diphenyliodonium abolishes lipopolysaccharide-induced down-regulation of transferrin receptor expression in N2a and BV-2 cells.

    abstract::The activation of cellular inflammatory response is tightly linked to induced production of reactive oxygen species (ROS) and nitric oxide (NO), which in turn have been identified as important regulators of cellular iron metabolism. In the present study, we have used the microglia cell line BV-2 and the neuroblastoma ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21005

    authors: Reis K,Hälldin J,Fernaeus S,Pettersson C,Land T

    更新日期:2006-10-01 00:00:00

  • Involvement of N-methyl-D-aspartate receptor subunits in zinc-mediated modification of CA1 long-term potentiation in the developing hippocampus.

    abstract::Zinc is an endogenous N-methyl-D-aspartate (NMDA) receptor blocker. It is possible that zinc-mediated modification of hippocampal CA1 long-term potentiation (LTP) is linked to the expression of NMDA receptor subunits, which varies with postnatal development. In the present study, the effect of ZnCl(2) and CaEDTA, a me...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22787

    authors: Takeda A,Itagaki K,Ando M,Oku N

    更新日期:2012-03-01 00:00:00

  • C1q, the recognition subcomponent of the classical pathway of complement, drives microglial activation.

    abstract::Microglia, central nervous system (CNS) resident phagocytic cells, persistently police the integrity of CNS tissue and respond to any kind of damage or pathophysiological changes. These cells sense and rapidly respond to danger and inflammatory signals by changing their cell morphology; by release of cytokines, chemok...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21875

    authors: Färber K,Cheung G,Mitchell D,Wallis R,Weihe E,Schwaeble W,Kettenmann H

    更新日期:2009-02-15 00:00:00

  • Some characteristics of muscle-conditioned media with excitatory and inhibitory influences on neurite outgrowth from early neural tube explants.

    abstract::This study examined the growth of basal plate explants from 40-hr chick embryos containing the trigeminal motor nucleus in the presence of target muscle-conditioned medium (MCM) from day-4, day-10, and day-18 embryos. We had previously found that MCM derived from the 4-day target tissue enhanced neurite outgrowth from...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490190109

    authors: Heaton MB

    更新日期:1988-01-01 00:00:00

  • Cell surface-mediated cellular interactions: effects of B104 neuroblastoma surface determinants on C6 glioma cellular properties.

    abstract::To study the influence of cell surface-associated molecules on intercellular communication, C6 glioma cells were cultured both on plastic and on substrata of paraformaldehyde-fixed B104 neuroblastoma cells. By then comparing the phenotypic expression of these "cocultured" C6 cells with cells cultured on tissue culture...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490070403

    authors: Ciment G,de Vellis J

    更新日期:1982-01-01 00:00:00

  • Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP.

    abstract::Calcium-activated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The muM and mM Ca(2+)-sensitive (mu- and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the latter step enabling removal of ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490290310

    authors: Banik NL,DeVries GH,Neuberger T,Russell T,Chakrabarti AK,Hogan EL

    更新日期:1991-07-01 00:00:00

  • Venom phospholipase A2-induced impairment of glutamate uptake: an indirect and nonselective effect related to phospholipid hydrolysis.

    abstract::In a nominally calcium-free medium, a toxic phospholipase A2, paradoxin, PDX (1-200nM) was able to significantly decrease glutamate uptake by rat hippocampal mini-slices. Under the same experimental conditions, PDX could also inhibit the reuptake of choline and dopamine, suggesting a nonselective action. Furthermore, ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19980201)51:3<349::AID-JNR

    authors: Dorandeu F,Antier D,Pernot-Marino I,Lapeyre P,Lallement G

    更新日期:1998-02-01 00:00:00

  • K+ channel properties in cultured mouse Schwann cells: dependence on extracellular K+.

    abstract::In cultured Schwann cells, single-channel and whole-cell K+ currents can be activated by depolarizing the membrane to values more negative than -50 mV. In elevated extracellular K+ concentration ([K+]o), however, single-channel activity and whole-cell currents could be recorded at more negative potentials. Thus, the t...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490280208

    authors: Verkhratsky A,Hoppe D,Kettenmann H

    更新日期:1991-02-01 00:00:00

  • Potentiation of synaptic strength and intrinsic excitability in the nucleus accumbens after 10 days of morphine withdrawal.

    abstract::Neuroadaptations in the nucleus accumbens (NAc) are associated with the development of drug addiction. Plasticity in synaptic strength and intrinsic excitability of NAc medium spiny neurons (MSNs) play critical roles in addiction induced by different classes of abused drugs. However, it is unknown whether morphine exp...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23025

    authors: Wu X,Shi M,Wei C,Yang M,Liu Y,Liu Z,Zhang X,Ren W

    更新日期:2012-06-01 00:00:00

  • Modulating astrogliosis after neurotrauma.

    abstract::Traumatic injury to the adult central nervous system (CNS) results in a rapid response from resident astrocytes, a process often referred to as reactive astrogliosis or glial scarring. The robust formation of the glial scar and its associated extracellular matrix (ECM) molecules have been suggested to interfere with a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/1097-4547(20010115)63:2<109::AID-JNR1002>3

    authors: McGraw J,Hiebert GW,Steeves JD

    更新日期:2001-01-15 00:00:00

  • Selection of reference genes for real-time quantitative reverse transcription-polymerase chain reaction in hippocampal structure in a murine model of temporal lobe epilepsy with focal seizures.

    abstract::Reference genes are often used to normalize expression of data from real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), and only a validation of their stability during a given experimental paradigm leads to reliable interpretations. The present study was thus designed to validate potentia...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22282

    authors: Pernot F,Dorandeu F,Beaup C,Peinnequin A

    更新日期:2010-04-01 00:00:00

  • Motor effects of cholinergic stimulation of the globus pallidus in rats.

    abstract::Forty-seven rats were cannulated permanently in either the globus pallidus or neostriatum and subsequently injected with carbachol, scopolamine, or carbachol plus scopolamine. The injection of carbachol (1 mug) into the globus pallidus produced transient hyperkinetic phasic dyskinesias (84%) followed in some cases by ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490010206

    authors: Springer P,Dill RE

    更新日期:1975-01-01 00:00:00

  • Characterization of neurotrophin receptors by affinity crosslinking.

    abstract::Neurotrophic factors regulate the developmental survival and differentiation of specific neuronal populations. Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are members of the nerve growth factor (NGF) protein family, also known as the neurotrophins. Insights into the different roles of neurotroph...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490340602

    authors: Escandón E,Burton LE,Szönyi E,Nikolics K

    更新日期:1993-04-15 00:00:00

  • Neuregulin-1 induces acetylcholine receptor transcription in the absence of GABPalpha phosphorylation.

    abstract::Localization of acetylcholine receptors (AChRs) to the postsynaptic region of muscle is mediated in part by transcriptional mechanisms, because the genes encoding AChR subunits are transcribed selectively in synaptic myofiber nuclei. Neuregulin-1 (NRG-1) is a synaptic signal and induces transcription of AChRs in muscl...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21563

    authors: Herndon CA,Fromm L

    更新日期:2008-04-01 00:00:00

  • Inhibition of K+ permeability diminishes alpha 2-adrenoceptor mediated effects on norepinephrine release.

    abstract::The effect of two different potassium channel blockers, 4-aminopyridine (4-AP) and quinine, on the alpha 2-adrenoceptor mediated modulation of norepinephrine (NE) release was investigated. Pairs of mouse vasa deferentia were loaded with 3H-norepinephrine (3H-NE), superfused continuously, and stimulated electrically. 4...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490200114

    authors: Zimanyi I,Folly G,Vizi ES

    更新日期:1988-05-01 00:00:00

  • Increased cerebral oxygen consumption in Eker rats and effects of N-methyl-D-aspartate blockade: Implications for autism.

    abstract::Because there is a strong correlation between tuberous sclerosis and autism, we used a tuberous sclerosis model (Eker rat) to test the hypothesis that these animals would have an altered regional cerebral O2 consumption that might be associated with autism. We also examined whether the altered cerebral O2 consumption ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21378

    authors: Weiss HR,Liu X,Zhang Q,Chi OZ

    更新日期:2007-08-15 00:00:00

  • Intracerebroventricular administration of GABA-A and GABA-B receptor antagonists attenuate feeding and sleeping-like behavior induced by L-pipecolic acid in neonatal chicks.

    abstract::It has been demonstrated that L-pipecolic acid (L-PA), a major metabolic intermediate of L-lysine (L-Lys) in the mammalian and chicken brain, is involved in the functioning of the GABAergic system. A previous study has shown that intracerebroventricular (i.c.v.) injection of L-PA suppressed feeding and induced sleep-l...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10656

    authors: Takagi T,Bungo T,Tachibana T,Saito ES,Saito S,Yamasaki I,Tomonaga S,Denbow DM,Furuse M

    更新日期:2003-07-15 00:00:00

  • Regulation of cAMP levels by protein kinase C in C6 rat glioma cells.

    abstract::Cultures of rat C6 rat glioma cells exhibit a diminished response to isoproterenol and forskolin after being treated with phorbol 12,13-dibutyrate (PDbU). An IC50 for PDbU of 38 +/- 5 nM and 62 +/- 8 nM was observed in the isoproterenol and forskolin response, respectively. Similarly, C6 cultures exhibited a diminishe...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490250110

    authors: Bressler JP,Tinsely P

    更新日期:1990-01-01 00:00:00

  • Atypical antipsychotics attenuate neurotoxicity of beta-amyloid(25-35) by modulating Bax and Bcl-X(l/s) expression and localization.

    abstract::We have demonstrated recently that atypical antipsychotics possess neuroprotective actions in H2O2-mediated and serum-withdrawal models of cell death. In the present study, we compared the ability of atypical and typical antipsychotics to protect against an insult mediated by Abeta(25-35), an apoptogenic fragment of t...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10832

    authors: Wei Z,Mousseau DD,Richardson JS,Dyck LE,Li XM

    更新日期:2003-12-15 00:00:00

  • Neurogenesis and glial proliferation are stimulated following diffuse traumatic brain injury in adult rats.

    abstract::Although increased neurogenesis has been described in rodent models of focal traumatic brain injury (TBI), the neurogenic response occurring after diffuse TBI uncomplicated by focal injury has not been examined to date, despite the pervasiveness of this distinct type of brain injury in the TBI patient population. Here...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22635

    authors: Bye N,Carron S,Han X,Agyapomaa D,Ng SY,Yan E,Rosenfeld JV,Morganti-Kossmann MC

    更新日期:2011-07-01 00:00:00

  • Growth factor treatment promotes mobilization of young but not aged adult subventricular zone precursors in response to demyelination.

    abstract::Precursor cells of the adult mouse subventricular zone (SVZ) are mobilized and recruited by a lysolecithin (LPC)-induced demyelination of the corpus callosum. Because age decreases the proliferation of the SVZ neural precursors as well as the potential for myelin repair of the adult central nervous system, we have com...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10411

    authors: Decker L,Picard-Riera N,Lachapelle F,Baron-Van Evercooren A

    更新日期:2002-09-15 00:00:00

  • Caspase-11 mediates ischemia-induced astrocyte death: involvement of endoplasmic reticulum stress and C/EBP homologous protein.

    abstract::Astrocytes are essential cells for maintaining brain integrity. We have recently shown that the transcription factor C/EBP homologous protein (CHOP), associated with endoplasmic reticulum (ER) stress, plays a key role in the astrocyte death induced by ischemia. Meanwhile, mediators of apoptosis downstream of CHOP in t...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22280

    authors: Fradejas N,Pastor MD,Burgos M,Beyaert R,Tranque P,Calvo S

    更新日期:2010-04-01 00:00:00

  • Antigen-specific adherence of antibody-producing hybridoma cells: application to neural and nonneural antigens.

    abstract::Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490150312

    authors: Najbauer J,Tigyi GJ,Nemeth P

    更新日期:1986-01-01 00:00:00

  • Endogenous aFGF expression and cellular changes after a demyelinating lesion in the spinal cord of adult normal mice: immunohistochemical study.

    abstract::Fibroblast growth factors (FGFs) are known to act on glial cells in vitro. At the present time, their involvement in the remyelinating process of the adult central nervous system (CNS) is still unknown. In the present study, using immunohistochemistry (IHC), we investigated the evolution in time and space of acidic FG...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490330107

    authors: Tourbah A,Baron-Van Evercooren A,Oliver L,Raulais D,Jeanny JC,Gumpel M

    更新日期:1992-09-01 00:00:00

  • Interferon-beta treatment of experimental autoimmune encephalomyelitis leads to rapid nonapoptotic termination of T cell infiltration.

    abstract::We investigated the possible mechanisms how interferon (IFN)-beta may control T cell infiltration in the CNS in experimental autoimmune encephalomyelitis (EAE). Adoptive transfer (AT) EAE was induced in groups of six female Lewis rats. Animals were treated with 3 x 10(5) units of recombinant rat IFN-beta s.c. once at ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1128

    authors: Schmidt J,Stürzebecher S,Toyka KV,Gold R

    更新日期:2001-07-01 00:00:00

  • High concentrations of cannabinoids activate apoptosis in human U373MG glioma cells.

    abstract::Cannabinoids bind to two G-protein-coupled receptors, CB1 and CB2, expressed by neurons and cells of the immune system, respectively. Glioma cells (astrocyte-derived brain tumor cells) express cannabinoid receptors, and numerous in vitro and in vivo studies performed in rodents have concluded that apoptosis could be i...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21757

    authors: Widmer M,Hanemann CO,Zajicek J

    更新日期:2008-11-01 00:00:00

  • Protective effect of L-trans-pyrrolidine-2,4-dicarboxilic acid preload against cell death induced by oxygen/glucose deprivation in differentiated PC12 cells.

    abstract::It has been postulated that cellular glutamate is released into the extracellular fluid when the energy supply of the brain is compromised (i.e., anoxia or oxygen/glucose deprivation), and there the amino acid triggers the so-called excitotoxic cascade, causing neuronal death. Several mechanisms for this release have ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20613

    authors: Martín ME,Muñoz FM,Dickinson DA,Forman HJ,Martín del Río R,Salinas M,Fando JL

    更新日期:2005-10-01 00:00:00

  • Cannabinoid receptor agonist-stimulated [35S]guanosine triphosphate gammaS binding in the brain of C57BL/6 and DBA/2 mice.

    abstract::The two inbred strains of mice C57BL/6 (alcohol-preferring) and DBA/2 (alcohol-avoiding) mice have been shown to differ significantly in their preference for alcohol (EtOH). We have previously demonstrated the differences in the density and the affinity of cannabinoid (CB1) receptors in the brains of the two inbred C5...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1094

    authors: Basavarajappa BS,Hungund BL

    更新日期:2001-05-15 00:00:00

  • Parenchymal pericytes are not the major contributor of extracellular matrix in the fibrotic scar after stroke in male mice.

    abstract::Scar formation after injury of the brain or spinal cord is a common event. While glial scar formation by astrocytes has been extensively studied, much less is known about the fibrotic scar, in particular after stroke. Platelet-derived growth factor receptor ß-expressing (PDGFRß+ ) pericytes have been suggested as a so...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24557

    authors: Roth M,Enström A,Aghabeick C,Carlsson R,Genové G,Paul G

    更新日期:2020-05-01 00:00:00

  • Involvement of endoplasmic reticulum stress in optic nerve degeneration after chronic high intraocular pressure in DBA/2J mice.

    abstract::DBA/2J mice are one of several animal strains used for experimental models of both intraocular hypertension and glaucoma. This study investigates the relationship between endoplasmic reticulum (ER) stress and optic nerve degeneration in DBA/2J mice. Intraocular pressure (IOP) was measured in DBA/2J mice between the ag...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23630

    authors: Ojino K,Shimazawa M,Izawa H,Nakano Y,Tsuruma K,Hara H

    更新日期:2015-11-01 00:00:00