Dexamethasone-conjugated DNA nanotubes as anti-inflammatory agents in vivo.


:The biopolymer DNA allows to create nanoscale, biocompatible structures, which can be designed in a target-specific and stimuli-responsive manner. DNA carrier systems with these characteristics hold a great potential for nanomedical applications, such as for the treatment of inflammatory diseases. Here we used a DNA-based drug carrier system for the pH-dependent delivery of the glucocorticoid dexamethasone into macrophages, a cell type with a key role in the regulation of inflammation. Dexamethasone (Dex) nanotubes were internalized within minutes by MH-S macrophages in vitro and by tissue resident macrophages in the mouse cremaster muscle in vivo and localized in their endosomes. Treatment with Dex nanotubes in vitro significantly reduced the LPS-induced TNF secretion by macrophages, as compared to equivalent amounts of free dexamethasone without affecting cell viability. Microinjection of Dex nanotubes into postischemic muscle tissue of anesthetized mice resulted in a marked reduction of ischemia-reperfusion-elicited leukocyte transmigration and diminished vascular expression of the endothelial adhesion molecules VCAM-1 and ICAM-1. Taken together, our results demonstrate that DNA nanotubes can be used as a platform for the targeted delivery of glucocorticoids and could thus foster the development of nanomedical therapeutics with reduced off-target effects.






Sellner S,Kocabey S,Zhang T,Nekolla K,Hutten S,Krombach F,Liedl T,Rehberg M




Has Abstract


2017-07-01 00:00:00












  • Aqueous solution behaviour and membrane disruptive activity of pH-responsive PEGylated pseudo-peptides and their intracellular distribution.

    abstract::The effect of PEGylation on the aqueous solution properties and cell membrane disruptive activity of a pH-responsive pseudo-peptide, poly(l-lysine iso-phthalamide), has been investigated by dynamic light scattering, haemolysis and lactate dehydrogenase (LDH) assays. Intracellular trafficking of the polymers has been e...


    pub_type: 杂志文章


    authors: Chen R,Yue Z,Eccleston ME,Slater NK

    更新日期:2008-11-01 00:00:00

  • Smooth muscle-like tissues engineered with bone marrow stromal cells.

    abstract::Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM alpha-acti...


    pub_type: 杂志文章


    authors: Cho SW,Kim IK,Lim SH,Kim DI,Kang SW,Kim SH,Kim YH,Lee EY,Choi CY,Kim BS

    更新日期:2004-07-01 00:00:00

  • Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing.

    abstract::Apart from partial or total joint replacement, no surgical procedure is currently available to treat large and deep cartilage defects associated with advanced diseases such as osteoarthritis. In this work, we developed a perfusion bioreactor system to engineer human cartilage grafts in a size with clinical relevance f...


    pub_type: 杂志文章


    authors: Santoro R,Olivares AL,Brans G,Wirz D,Longinotti C,Lacroix D,Martin I,Wendt D

    更新日期:2010-12-01 00:00:00

  • Peptide targeted tripod macrocyclic Gd(III) chelates for cancer molecular MRI.

    abstract::Rational design and develop of targeted contrast agents binding to cancer-related proteins will achieve more accurate cancer diagnosis and prognosis by magnetic resonance (MR) imaging. CREKA is a tumor-homing pentapeptide (Cys-Arg-Glu-Lys-Ala) specifically homes to fibrin-fibronectin complexes abundantly expressed in ...


    pub_type: 杂志文章


    authors: Zhou Z,Wu X,Kresak A,Griswold M,Lu ZR

    更新日期:2013-10-01 00:00:00

  • Augmented glioma-targeted theranostics using multifunctional polymer-coated carbon nanodots.

    abstract::Overcoming biological barriers to imaging-guided site-specific delivery of therapeutics is the goal of current nanomedicine designs. Here, multifunctional polymer-coated carbon nanodots with an interleukin-6 (IL-6) fragment peptide for receptor-targeting (pCDPI) were prepared for drug delivery. The pCDPI exhibits smal...


    pub_type: 杂志文章


    authors: Wang S,Li C,Qian M,Jiang H,Shi W,Chen J,Lächelt U,Wagner E,Lu W,Wang Y,Huang R

    更新日期:2017-10-01 00:00:00

  • Synergy between IL-6 and soluble IL-6 receptor enhances bone morphogenetic protein-2/absorbable collagen sponge-induced bone regeneration via regulation of BMPRIA distribution and degradation.

    abstract::Bone morphogenetic protein-2/absorbable collagen sponge (BMP-2/ACS) implants have been approved for clinical use to induce bone regeneration. We previously showed that exaggerated inflammation characterized by elevated level of inflammatory cytokines including TNF-α, IL-1β, and IL-6 has been shown to inhibit BMP-2/ACS...


    pub_type: 杂志文章


    authors: Huang RL,Chen G,Wang W,Herller T,Xie Y,Gu B,Li Q

    更新日期:2015-10-01 00:00:00

  • Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment.

    abstract::Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer ste...


    pub_type: 杂志文章


    authors: Landberg G,Fitzpatrick P,Isakson P,Jonasson E,Karlsson J,Larsson E,Svanström A,Rafnsdottir S,Persson E,Gustafsson A,Andersson D,Rosendahl J,Petronis S,Ranji P,Gregersson P,Magnusson Y,Håkansson J,Ståhlberg A

    更新日期:2020-03-01 00:00:00

  • Characterization of a hyaluronic acid-Arg-Gly-Asp peptide cell attachment matrix.

    abstract::We have developed a method to modify cross-linked hyaluronic acid with peptides containing the Arg-Gly-Asp sequence. The material created by this process is a three-dimensional porous matrix capable of supporting integrin receptor-mediated cell attachment. Peptide density can be controlled by varying the reaction cond...


    pub_type: 杂志文章


    authors: Glass JR,Dickerson KT,Stecker K,Polarek JW

    更新日期:1996-06-01 00:00:00

  • Growth of hollow cell spheroids in microbead templated chambers.

    abstract::Cells form hollow, spheroidal structures during the development of many tissues, including the ocular lens, inner ear, and many glands. Therefore, techniques for in vitro formation of hollow spheroids are valued for studying developmental and disease processes. Current in vitro methods require cells to self-organize i...


    pub_type: 杂志文章


    authors: Wang E,Wang D,Geng A,Seo R,Gong X

    更新日期:2017-10-01 00:00:00

  • In vivo and in vitro degradation of monofilament absorbable sutures, PDS and Maxon.

    abstract::Two new absorbable monofilament suture materials polydioxanone and Maxon are being employed increasingly in abdominal surgery because of increased strength retention and decreased tissue reactivity compared with previously available materials. As part of our investigation of the behaviour of suture materials, 3-0 sutu...


    pub_type: 杂志文章


    authors: Metz SA,Chegini N,Masterson BJ

    更新日期:1990-01-01 00:00:00

  • Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions.

    abstract::Silver nanoparticles (AgNPs) have anti-cancer effect. However, whether and how these particles could inhibit the growth of acute myeloid leukemia (AML) cells is unclear. In the present study, we prepared AgNPs with various sizes and investigated their cytotoxic effect on AML cells. We found that AgNPs could inhibit th...


    pub_type: 杂志文章


    authors: Guo D,Zhu L,Huang Z,Zhou H,Ge Y,Ma W,Wu J,Zhang X,Zhou X,Zhang Y,Zhao Y,Gu N

    更新日期:2013-10-01 00:00:00

  • Adhesion and proliferation of human endothelial cells on photochemically modified polytetrafluoroethylene.

    abstract::We studied the adhesion and proliferation of human endothelial cells on photochemically modified polytetrafluoroethylene samples. The polymer surfaces were modified by exposure to the ultraviolet light of a Xe(2)(*)-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere. Treatment times were between 10 and 20...


    pub_type: 杂志文章


    authors: Gumpenberger T,Heitz J,Bäuerle D,Kahr H,Graz I,Romanin C,Svorcik V,Leisch F

    更新日期:2003-12-01 00:00:00

  • Control of the formation of vascular networks in 3D tissue engineered constructs.

    abstract::Construction of bio-mimetic well-organized three-dimensional (3D) tissue with various cells in vitro is one of the ultimate goals of tissue engineering. In particular, fabrication of vasculature in 3D tissue is one of the most important tasks in tissue engineering, because a vascular network is indispensable for almos...


    pub_type: 杂志文章


    authors: Muraoka M,Shimizu T,Itoga K,Takahashi H,Okano T

    更新日期:2013-01-01 00:00:00

  • Surfactant functionalization induces robust, differential adhesion of tumor cells and blood cells to charged nanotube-coated biomaterials under flow.

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    pub_type: 杂志文章


    authors: Mitchell MJ,Castellanos CA,King MR

    更新日期:2015-07-01 00:00:00

  • Tissue engineered bone mimetics to study bone disorders ex vivo: Role of bioinspired materials.

    abstract::Recent advances in materials development and tissue engineering has resulted in a substantial number of bioinspired materials that recapitulate cardinal features of bone extracellular matrix (ECM) such as dynamic inorganic and organic environment(s), hierarchical organization, and topographical features. Bone mimickin...


    pub_type: 杂志文章,评审


    authors: Shih YV,Varghese S

    更新日期:2019-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Chou MT,Chang SN,Ke C,Chang HI,Sung ML,Kuo HC,Chen CN

    更新日期:2010-05-01 00:00:00

  • Enhanced Bax upregulating in mitochondria for deep tumor therapy based on SO2 prodrug loaded Au-Ag hollow nanotriangles.

    abstract::Nowadays, limited deep tumor penetration and lower therapeutic effect are still the major obstacle in nanomedicines for cancer therapy. Here, we developed high-efficiency nanocomposites, SO2 prodrug (BTS) loaded Au-Ag hollow nanotriangles (Au-Ag-BTS HTNs), which could not only synergistically upregulate Bax expression...


    pub_type: 杂志文章


    authors: Xu M,Lu Q,Song Y,Yang L,Li J,Li N

    更新日期:2020-08-01 00:00:00

  • Cell adhesion on phase-separated surface of block copolymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) and poly(dimethylsiloxane).

    abstract::We investigated the morphological effect of phase-separated block copolymer surfaces composed of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and poly(dimethylsiloxane) (PDMS) on protein adsorption and cell adhesion behavior. We observed three different types of phase-separated surface morphologies by T...


    pub_type: 杂志文章


    authors: Seo JH,Matsuno R,Takai M,Ishihara K

    更新日期:2009-10-01 00:00:00

  • Meniscal repair by fibrocartilage in the dog: characterization of the repair tissue and the role of vascularity.

    abstract::Lesions in the avascular part of 20 canine menisci were repaired by implantation of a porous polyurethane. Seven menisci were not repaired and served as controls. The repair tissue was characterized by biochemical and immunological analysis. The role of vascularity in healing was studied by perfusion of menisci with I...


    pub_type: 杂志文章


    authors: Klompmaker J,Veth RP,Jansen HW,Nielsen HK,de Groot JH,Pennings AJ,Kuijer R

    更新日期:1996-09-01 00:00:00

  • Three reversible and controllable discrete steps of channel gating of a viral DNA packaging motor.

    abstract::The channel of the viral DNA packaging motor allows dsDNA to enter the protein procapsid shell during maturation and to exit during infection. We recently showed that the bacteriophage phi29 DNA packaging motor exercises a one-way traffic property using a channel as a valve for dsDNA translocation. This raises a quest...


    pub_type: 杂志文章


    authors: Geng J,Fang H,Haque F,Zhang L,Guo P

    更新日期:2011-11-01 00:00:00

  • The effect of exposure conditions upon the release of soluble copper and tin from dental amalgams.

    abstract::The concentrations of soluble copper and tin which were generated by the corrosion of a number of different amalgams in both an artificial saliva and Ringer's solution are reported. Besides variations in solution composition, the effects of solution agitation and abrasive particle wear upon the release of soluble spec...


    pub_type: 杂志文章


    authors: Mueller HJ,Edahl A

    更新日期:1984-07-01 00:00:00

  • The significance of pore microarchitecture in a multi-layered elastomeric scaffold for contractile cardiac muscle constructs.

    abstract::Multi-layered poly(glycerol-sebacate) (PGS) scaffolds with controlled pore microarchitectures were fabricated, combined with heart cells, and cultured with perfusion to engineer contractile cardiac muscle constructs. First, one-layered (1L) scaffolds with accordion-like honeycomb shaped pores and elastomeric mechanica...


    pub_type: 杂志文章


    authors: Park H,Larson BL,Guillemette MD,Jain SR,Hua C,Engelmayr GC Jr,Freed LE

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  • Surface characteristics and the interaction of colloidal particles with mouse peritoneal macrophages.

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    pub_type: 杂志文章


    authors: Illum L,Jacobsen LO,Müller RH,Mak E,Davis SS

    更新日期:1987-03-01 00:00:00

  • Hyaluronic acid-shelled acid-activatable paclitaxel prodrug micelles effectively target and treat CD44-overexpressing human breast tumor xenografts in vivo.

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    pub_type: 杂志文章


    authors: Zhong Y,Goltsche K,Cheng L,Xie F,Meng F,Deng C,Zhong Z,Haag R

    更新日期:2016-04-01 00:00:00

  • Antibody and cytokine-associated immune responses to S. equi antigens entrapped in PLA nanospheres.

    abstract::Strangles is an infectious disease caused by Streptococcus equi subspecies equi that affects the upper respiratory tract of the Equidae. The control of this disease seems to be dependent on its earlier detection and prevention, but prolonged animal protection without development of strong and severe side effects has n...


    pub_type: 杂志文章


    authors: Florindo HF,Pandit S,Gonçalves LM,Videira M,Alpar O,Almeida AJ

    更新日期:2009-10-01 00:00:00

  • Drug release characteristics of phase separation pHEMA sponge materials.

    abstract::A number of phase separation pHEMA sponge hydrogels have been prepared based on variations in monomer contents, concentration of cross-linking agent, solvent mixture and temperature of polymerization. The loading levels and release profiles of the anti-inflammatory drug prednisolone were examined for each of the pHEMA...


    pub_type: 杂志文章


    authors: Lou X,Munro S,Wang S

    更新日期:2004-09-01 00:00:00

  • Degradation of polydispersed poly(L-lactic acid) to modulate lactic acid release.

    abstract::Polydispersed poly(L-lactic acid) (PLLA) membranes comprised of blends of monodispersed PLLA of weight average molecular weight of 82,500 and 7600 were fabricated to investigate the effect of polydispersity on degradation characteristics. The PLLA blends exhibited large spherulites of high molecular weight chains embe...


    pub_type: 杂志文章


    authors: von Recum HA,Cleek RL,Eskin SG,Mikos AG

    更新日期:1995-04-01 00:00:00

  • Effects of caspase-1 knockout on chronic neural recording quality and longevity: insight into cellular and molecular mechanisms of the reactive tissue response.

    abstract::Chronic implantation of microelectrodes into the cortex has been shown to lead to inflammatory gliosis and neuronal loss in the microenvironment immediately surrounding the probe, a hypothesized cause of neural recording failure. Caspase-1 (aka Interleukin 1β converting enzyme) is known to play a key role in both infl...


    pub_type: 杂志文章


    authors: Kozai TD,Li X,Bodily LM,Caparosa EM,Zenonos GA,Carlisle DL,Friedlander RM,Cui XT

    更新日期:2014-12-01 00:00:00

  • Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis.

    abstract::The effective role of exosome delivering neurogenic microRNA (miRNA) enables to induce efficient differentiation process during neurogenesis. The microfludic system capable of visualizing the exosomal behavior such as secretion, migration, and uptake of individual exosomes can be used as a robust technique to understa...


    pub_type: 杂志文章


    authors: Oh HJ,Shin Y,Chung S,Hwang DW,Lee DS

    更新日期:2017-01-01 00:00:00

  • PEGylated TNF-related apoptosis-inducing ligand (TRAIL) for effective tumor combination therapy.

    abstract::Although PEGylated TNF-related apoptosis-inducing ligand (PEG-TRAIL) has good tumor cell specificity and stability, its therapeutic potential is restricted by the development of tumor cell resistance. The purpose of this study was to develop an effective combination therapy with sustained biological activity based on ...


    pub_type: 杂志文章


    authors: Jiang HH,Kim TH,Lee S,Chen X,Youn YS,Lee KC

    更新日期:2011-11-01 00:00:00