Precision toxicology based on single cell sequencing: an evolving trend in toxicological evaluations and mechanism exploration.

Abstract:

:In this review, we introduce a new concept, precision toxicology: the mode of action of chemical- or drug-induced toxicity can be sensitively and specifically investigated by isolating a small group of cells or even a single cell with typical phenotype of interest followed by a single cell sequencing-based analysis. Precision toxicology can contribute to the better detection of subtle intracellular changes in response to exogenous substrates, and thus help researchers find solutions to control or relieve the toxicological effects that are serious threats to human health. We give examples for single cell isolation and recommend laser capture microdissection for in vivo studies and flow cytometric sorting for in vitro studies. In addition, we introduce the procedures for single cell sequencing and describe the expected application of these techniques to toxicological evaluations and mechanism exploration, which we believe will become a trend in toxicology.

journal_name

Arch Toxicol

journal_title

Archives of toxicology

authors

Zhang B,Huang K,Zhu L,Luo Y,Xu W

doi

10.1007/s00204-017-1971-4

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

2539-2549

issue

7

eissn

0340-5761

issn

1432-0738

pii

10.1007/s00204-017-1971-4

journal_volume

91

pub_type

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