Precision toxicology based on single cell sequencing: an evolving trend in toxicological evaluations and mechanism exploration.


:In this review, we introduce a new concept, precision toxicology: the mode of action of chemical- or drug-induced toxicity can be sensitively and specifically investigated by isolating a small group of cells or even a single cell with typical phenotype of interest followed by a single cell sequencing-based analysis. Precision toxicology can contribute to the better detection of subtle intracellular changes in response to exogenous substrates, and thus help researchers find solutions to control or relieve the toxicological effects that are serious threats to human health. We give examples for single cell isolation and recommend laser capture microdissection for in vivo studies and flow cytometric sorting for in vitro studies. In addition, we introduce the procedures for single cell sequencing and describe the expected application of these techniques to toxicological evaluations and mechanism exploration, which we believe will become a trend in toxicology.


Arch Toxicol


Archives of toxicology


Zhang B,Huang K,Zhu L,Luo Y,Xu W




Has Abstract


2017-07-01 00:00:00














  • Transforming growth factor-beta1 is not involved in TCDD-dependent release from contact inhibition in WB-F344 cells.

    abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most potent tumor promoter ever tested in rodents. Although it is known that most of the effects of TCDD are mediated by binding to the aryl hydrocarbon receptor (AhR), the mechanisms leading to tumor promotion still remain to be elucidated. Loss of contact inhibition ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Hoelper P,Faust D,Oesch F,Dietrich C

    更新日期:2005-01-01 00:00:00

  • Use of M-mode and Doppler echocardiography to investigate the cardiotoxicity of minoxidil in beagle dogs.

    abstract::Doppler and M-mode echocardiography (EC) were used to investigate the effects of minoxidil on the cardiac function of the dog and potentially to clarify the pathogenesis of cardiac lesions, in particular the necrotic lesion in the left ventricle and the haemorrhagic lesion in the right atrium. Groups of three dogs wer...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Hanton G,Gautier M,Bonnet P

    更新日期:2004-01-01 00:00:00

  • Toxicology of microcystins with reference to cases of human intoxications and epidemiological investigations of exposures to cyanobacteria and cyanotoxins.

    abstract::Blooms of cyanobacteria have been documented throughout history, all over the world. Mass populations of these organisms typically present hazards to human health and are known for the production of a wide range of highly toxic metabolites-cyanotoxins, of which among the most common and most investigated are the micro...

    journal_title:Archives of toxicology

    pub_type: 杂志文章,评审


    authors: Svirčev Z,Drobac D,Tokodi N,Mijović B,Codd GA,Meriluoto J

    更新日期:2017-02-01 00:00:00

  • Mutagenicity test systems for the detection of chromosome aberrations in vivo.

    abstract::In mutagenicity testing of pharmaceuticals it is advisable, whenever possible, to use in vivo mammalian systems where the pharmacokinetic properties of a drug would be more or less similar to those in man. However, although there are a number of different genetic endpoints which are important in mutagen testing, suffi...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Matter BE,Tsuchimoto T

    更新日期:1980-11-01 00:00:00

  • Deficit in learning and memory of rats with chronic fluorosis correlates with the decreased expressions of M1 and M3 muscarinic acetylcholine receptors.

    abstract::To reveal the molecular mechanism of deficit in learning and memory induced by chronic fluorosis, the expression of muscarinic acetylcholine receptors (mAChRs) and oxidative stress were investigated. Sixty Sprague-Dawley (SD) rats were divided randomly into two groups (30 cases in each), i.e., the control group (<0.5 ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Dong YT,Wang Y,Wei N,Zhang QF,Guan ZZ

    更新日期:2015-11-01 00:00:00

  • Hematological toxicity of tetrachloroethylene in mice.

    abstract::Female C57/BL/6 x DBA/2 hybrid mice were exposed to two concentrations (270 and 135 ppm) of tetrachloroethylene (PER) in inhalation chambers for 6 h/day, 5 days per week, up to 11.5 weeks (270 ppm) and 7.5 weeks (135 ppm), respectively, followed by a 3-week exposure-free period. In the peripheral blood a reduction of ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Seidel HJ,Weber L,Barthel E

    更新日期:1992-01-01 00:00:00

  • Effect of osmotic diuresis on gentamicin-induced nephrotoxicity in rats.

    abstract::The effect of isosorbide diuresis on gentamicin excretion and tissue accumulation as well as gentamicin-induced histopathologic changes in renal tissue were examine in rats. Gentamicin (30, 60, 120, 160 mg/kg) was administered s.c. for 10 consecutive days with or without isosorbide pretreatment. Osmotic diuresis with ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Newman RA,Weinstock LB,Gump DW,Hacker MP,Yates JW

    更新日期:1980-09-01 00:00:00

  • Sexual dimorphism of cadmium-induced toxicity in rats: involvement of sex hormones.

    abstract::The toxic effect of cadmium varies with sex in experimental animals. Previous studies have demonstrated that pretreatment of male Fischer 344 (F344) rats with the female sex hormone progesterone markedly enhances the susceptibility to cadmium, suggesting a role for progesterone in the sexual dimorphism of cadmium toxi...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Shimada H,Hashiguchi T,Yasutake A,Waalkes MP,Imamura Y

    更新日期:2012-09-01 00:00:00

  • Cadmium chloride inhibits lactate gluconeogenesis in isolated human renal proximal tubules: a cellular metabolomic approach with 13C-NMR.

    abstract::As part of a study on cadmium nephrotoxicity, we studied the effect of cadmium chloride (CdCl2) in isolated human renal proximal tubules metabolizing the physiological substrate lactate. Dose-effect experiments showed that 10-500 μM CdCl2 reduced lactate removal, glucose production and the cellular levels of ATP, coen...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Faiz H,Conjard-Duplany A,Boghossian M,Martin G,Baverel G,Ferrier B

    更新日期:2011-09-01 00:00:00

  • Investigation on the metabolic degradation of the side chain of furazolidone.

    abstract::The investigation was aimed at providing insight into the side chain metabolism of furazolidone in mice. The agents used in the experiments were furazolidone, 3-amino-2-oxazolidinone, oxazolidinone, nitrofurantoin, 3-aminohydantoin and hydantoin, administered intraperitoneally at five equimolar doses ranging from 0.17...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


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    更新日期:1987-12-01 00:00:00

  • Alterations in neurofilaments content and calpains activity of sciatic nerve of carbon disulfide-treated rats.

    abstract::Chronic exposure to carbon disulfide (CS2) can induce polyneuropathy in occupational worker and experimental animals, but underlying mechanism for CS2 neurotoxicity is currently unknown. In the present study, male Wistar rats were randomly divided into two experimental groups and one control group. The rats in two exp...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Song F,Zhang C,Wang Q,Zeng T,Xie K

    更新日期:2009-06-01 00:00:00

  • Toxicology data of graphene-family nanomaterials: an update.

    abstract::Due to its unique physical structure and chemical properties, graphene family nanomaterials (GFNs) and derived commodities have been widely used in commercial products, particularly biomedical applications, which has significantly increased the risk of human exposure. There exists significant evidence that GFNs are ac...

    journal_title:Archives of toxicology

    pub_type: 杂志文章,评审


    authors: Xiaoli F,Qiyue C,Weihong G,Yaqing Z,Chen H,Junrong W,Longquan S

    更新日期:2020-06-01 00:00:00

  • Exposures to the environmental toxicants pentachlorophenol (PCP) and dichlorodiphenyltrichloroethane (DDT) modify secretion of interleukin 1-beta (IL-1β) from human immune cells.

    abstract::Pentachlorophenol (PCP) and Dichlorodiphenyltrichloroethane (DDT) are environmental contaminants found in human blood. Previous studies have shown that PCP and DDT inhibit the lytic function of highly purified human natural killer (NK) lymphocytes and decrease the expression of several surface proteins on NK cells. In...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Martin TJ,Whalen MM

    更新日期:2017-04-01 00:00:00

  • Bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol by sulfotransferases from human, mouse and rat: a comparative study.

    abstract::5-Hydroxymethylfurfural (HMF) and furfuryl alcohol (FFA) are moderately potent rodent carcinogens that are present in thermally processed foodstuffs. The carcinogenic effects were hypothesized to originate from sulfotransferase (SULT)-mediated bioactivation yielding DNA-reactive and mutagenic sulfate esters, a confirm...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Sachse B,Meinl W,Sommer Y,Glatt H,Seidel A,Monien BH

    更新日期:2016-01-01 00:00:00

  • Effects of fluoroquinolones and magnesium deficiency in murine limb bud cultures.

    abstract::Quinolone-induced arthropathy is probably caused by a lack of functionally available magnesium in immature joint cartilage. We used an in vitro assay to study the effects of fluoroquinolones on cartilage formation in mouse limb buds from 12-day-old mouse embryos in regular and in magnesium-deficient medium. Omission o...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Förster C,Rücker M,Shakibaei M,Baumann-Wilschke I,Vormann J,Stahlmann R

    更新日期:1998-06-01 00:00:00

  • Glutaminases regulate glutathione and oxidative stress in cancer.

    abstract::Targeted therapies against cancer have improved both survival and quality of life of patients. However, metabolic rewiring evokes cellular mechanisms that reduce therapeutic mightiness. Resistant cells generate more glutathione, elicit nuclear factor erythroid 2-related factor 2 (NRF2) activation, and overexpress many...

    journal_title:Archives of toxicology

    pub_type: 杂志文章,评审


    authors: Matés JM,Campos-Sandoval JA,de Los Santos-Jiménez J,Márquez J

    更新日期:2020-08-01 00:00:00

  • Gestational bisphenol S impairs placental endocrine function and the fusogenic trophoblast signaling pathway.

    abstract::Exposure to bisphenolic chemicals during pregnancy occurs in > 90% of pregnancies. Bisphenolic compounds can cross the placental barrier reaching fetal circulation. However, the effects of emerging bisphenolic compounds, such as bisphenol S (BPS), on placental function remain untested. The aim was to determine if bisp...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Gingrich J,Pu Y,Roberts J,Karthikraj R,Kannan K,Ehrhardt R,Veiga-Lopez A

    更新日期:2018-05-01 00:00:00

  • Inhibition, reactivation and aging kinetics of cyclohexylmethylphosphonofluoridate-inhibited human cholinesterases.

    abstract::Cyclohexylmethylphosphonofluoridate (cyclosarin) is a highly toxic organophosphate, which was shown to be rather resistant to conventional oxime therapy. To give more insight into the inhibition, reactivation and aging kinetics, human acetyl-(AChE) and butyrylcholinesterase (BChE) were inhibited by cyclosarin (k2 of 7...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Worek F,Eyer P,Szinicz L

    更新日期:1998-09-01 00:00:00

  • Mutagenicity and cytotoxicity of 2-chlorobenzylidene malonitrile (CS) and metabolites in V79 Chinese hamster cells.

    abstract::The genotoxicity of the sensory irritant 2-chlorobenzylidene malonitrile (CS) to V79 Chinese hamster cells was investigated using the induction of gene mutations, micronuclei and DNA repair synthesis as biological endpoints. CS efficiently induced micronuclei and mutants resistant to 6-thioguanine in these cells, but ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Ziegler-Skylakakis K,Summer KH,Andrae U

    更新日期:1989-01-01 00:00:00

  • Cardiotoxicity of chlorodibromomethane and trichloromethane in rats and isolated rat cardiac myocytes.

    abstract::The cardiovascular effects were investigated after acute and subacute treatment with chlorodibromomethane (CDBM; 0.4 to 3.2 mmol/kg p.o.), trichloromethane (TCM; 0.31 and 1.25 mmol/kg p.o.) and mixtures of CDBM and TCM (acute, 0.8 mmol CDBM/kg + 1.25 mmol TCM/kg p.o.; subacute, 0.4 mmol CDBM/kg + 0.31 mmol TCM/kg p.o....

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Müller SP,Wolna P,Wünscher U,Pankow D

    更新日期:1997-01-01 00:00:00

  • Selectivity of plasma membrane calcium ATPase (PMCA)-mediated extrusion of toxic divalent cations in vitro and in cultured cells.

    abstract::In the recent years, the toxicity of certain divalent cations has been associated with the alteration of intracellular Ca2+ homeostasis. Among other mechanisms, these cations may affect the functionality of certain Ca2+-binding proteins and/or Ca2+ pumps. The plasma membrane calcium pump (PMCA) maintains Ca2+ homeosta...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Ferreira-Gomes MS,Mangialavori IC,Ontiveros MQ,Rinaldi DE,Martiarena J,Verstraeten SV,Rossi JPFC

    更新日期:2018-01-01 00:00:00

  • The closed chamber technique--uptake, endogenous production, excretion, steady-state kinetics and rates of metabolism of gases and vapors.

    abstract::The "closed chamber technique" (CCT) is presented. It allows investigation of pharmacokinetics of volatile substances in vivo in animals and in man and in vitro using tissue fractions. During the exposure period only the atmospheric concentrations of the substance are measured. The concentration-time data obtained are...

    journal_title:Archives of toxicology

    pub_type: 杂志文章,评审


    authors: Filser JG

    更新日期:1992-01-01 00:00:00

  • Fasting for 24 h reveals liver microsteatosis after continuous i.v. infusion of milacemide in the rat.

    abstract::Milacemide (2-n-pentylaminoacetamide) hydrochloride was administered by continuous i.v. infusion for up to 7 days, at 300 and 600 mg/kg per day to male Sprague-Dawley rats. This was intended to provide high and sustained exposure to evaluate the effect of a preterminal 24-h fast on liver lipid content. Liver lipid con...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Rakotoamboa JL,Masson M,Palate B,Carleer J,Roba J

    更新日期:1994-01-01 00:00:00

  • Metabolomics reveals trichloroacetate as a major contributor to trichloroethylene-induced metabolic alterations in mouse urine and serum.

    abstract::Trichloroethylene (TCE)-induced liver toxicity and carcinogenesis is believed to be mediated in part by activation of the peroxisome proliferator-activated receptor α (PPARα). However, the contribution of the two TCE metabolites, dichloroacetate (DCA) and trichloroacetate (TCA) to the toxicity of TCE, remains unclear....

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Fang ZZ,Krausz KW,Tanaka N,Li F,Qu A,Idle JR,Gonzalez FJ

    更新日期:2013-11-01 00:00:00

  • Birth weights of newborns and pregnancy outcomes of environmentally boron-exposed females in Turkey.

    abstract::Boric acid and sodium borates are currently classified as being toxic to reproduction under "Category 1B" with the hazard statement of "H360 FD" in the European CLP regulation. This has prompted studies on boron-mediated reprotoxic effects in male workers in boron mining areas and boric acid production plants. By cont...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Duydu Y,Başaran N,Üstündağ A,Aydın S,Yalçın CÖ,Anlar HG,Bacanlı M,Aydos K,Atabekoğlu CS,Golka K,Ickstadt K,Schwerdtle T,Werner M,Meyer S,Bolt HM

    更新日期:2018-08-01 00:00:00

  • Determination of urinary thymidine glycol using affinity chromatography, HPLC and post-column reaction detection: a biomarker of oxidative DNA damage upon kidney transplantation.

    abstract::Reactive oxygen species are generated during ischaemia-reperfusion of tissue. Oxidation of thymidine by hydroxyl radicals (HO) leads to the formation of 5,6-dihydroxy-5,6-dihydrothymidine (thymidine glycol). Thymidine glycol is excreted in urine and can be used as biomarker of oxidative DNA damage. Time dependent chan...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Thier R,Brüning T,Kocher K,Blaszkewicz M,Makropoulos V,Sundberg A,Bolt HM

    更新日期:1999-11-01 00:00:00

  • Effects of bisphenol A on breast cancer and its risk factors.

    abstract::The incidence of breast cancer in Korea has been increasing for the last two decades (1983-2005), and now, breast cancer is ranked the leading cause of cancer in Korean women. Along with other endocrine disrupting chemicals (EDCs), bisphenol A (BPA) has been suspected as a potential risk factor for breast cancer. We s...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Yang M,Ryu JH,Jeon R,Kang D,Yoo KY

    更新日期:2009-03-01 00:00:00

  • Inhibition of poly(ADP-ribose) polymerase (PARP) influences the mode of sulfur mustard (SM)-induced cell death in HaCaT cells.

    abstract::Sulfur mustard (SM) is a bifunctional alkylating agent. Its primary toxic consequence is severe skin damage with blisters, occurring after skin contact. These vesicant properties of SM have been linked to cell death of proliferating keratinocytes in the basal layer of the skin. Catalytic activation of the nuclear enzy...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Kehe K,Raithel K,Kreppel H,Jochum M,Worek F,Thiermann H

    更新日期:2008-07-01 00:00:00

  • [Delayed implantation and early developmental defects in the mouse caused by PCB: 2,2'-dichlorobiphenyl (author's transl)].

    abstract::Pregnant mice administered per os during the days 1--3 (plug day = day 0) post conception (p.c.) with 375 mg 2,2'-dichlorobiphenyl/kilograms/day revealed on day 18 p.c. increased number of resorptions and doses up from 500 milligrams/kilograms/day led to a substantial retardation of the prenatal development. Based on ...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Török P

    更新日期:1978-08-09 00:00:00

  • Structure-toxicity relationship of ethylene glycol ethers.

    abstract::The ultimate purpose of the present study was to evaluate correlations between acute in vivo and in vitro toxicity and log P (P is n-octanol-water partition coefficient). The in vitro toxicity to cloned cells (neuroblastoma N18TG-2 and glioma C6) in culture (ED50) and the in vivo toxicity to mice (LD50) of ethylene gl...

    journal_title:Archives of toxicology

    pub_type: 杂志文章


    authors: Tanii H,Saito S,Hashimoto K

    更新日期:1992-01-01 00:00:00