Production of antibodies against murine class II antigens using unconjugated synthetic peptides of the beta chain region 63-78.


:synthetic peptides corresponding to residues 63-78 of the first domain of the beta chain of murine I-A/I-E class II antigens were used in the unconjugated rather than the traditional protein-conjugated form to immunize (129J X B6)F1 mice. The sequences made represented the four haplotypes; Ak beta, Ad beta, Abm-12 beta and Ed beta. These sequences were selected on the basis of computer algorithms used to predict surface accessibility and main-chain flexibility profiles, and by reported hypervariability and site-directed mutagenesis experiments of these regions. Factors such as the use of complete Freund's adjuvant, a continuous immunization regime, and the sex of the mice used were found to influence the amount of anti-peptide antibody produced when unconjugated peptide was used as the immunogen. Antibodies produced were shown by FACS analysis to react with I-A/I-E class II antigens expressed on the lymphocyte surface. These findings indicate that the use of unconjugated synthetic peptides representing sequences of proteins which are in close proximity to disulfide bonds may be an advantage over conventional methods of peptide conjugation to proteins.


J Immunol Methods


McCormick DJ,Hillman K,David CS




Has Abstract


1988-10-26 00:00:00














  • A rapid method for isolation of antigenically active human cell surface antigens associated with beta 2-microglobulin using a monoclonal antibody.

    abstract::Serologically and biochemically pure HLA antigens and other beta 2-microglobulin-associated proteins were isolated using a rapid immunoprecipitation technique with a monoclonal anti-beta 2-microglobulin reagent A88. Elution of the immunoprecipitate with beta 2-microglobulin isolated from kidney patients proved to be e...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: LeClair K,Van Agthoven A,Terhorst C

    更新日期:1981-01-01 00:00:00

  • Analysis of lymphocyte aggregation using digital image analysis.

    abstract::We present the development and testing of a novel assay of lymphocyte adhesion based on time-resolved morphological measurements of intercellular aggregation. Homotypic lymphocyte aggregation is induced according to various protocols and monitored for several hours using video microscopy and time-lapse recording. Digi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Munn LL,Glacken MW,McIntyre BW,Zygourakis K

    更新日期:1993-11-05 00:00:00

  • Rapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays.

    abstract::An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange s...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Luk JM,Lindberg AA

    更新日期:1991-03-01 00:00:00

  • Generation of peptide-specific CD8+ T cells by phytohemagglutinin-stimulated antigen-mRNA-transduced CD4+ T cells.

    abstract::Functional analysis of antigen-specific CD8(+) T cells is important for understanding the immune response in various immunological disorders. To analyze CD8(+) T cell responses to a variety of antigens with no readily defined peptides available, we developed a system using CD4(+) phytohemagglutinin (PHA) blasts transd...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Naota H,Miyahara Y,Okumura S,Kuzushima K,Akatsuka Y,Hiasa A,Kitano S,Takahashi T,Yuta A,Majima Y,Shiku H

    更新日期:2006-07-31 00:00:00

  • Scanning tunnelling microscopy and dynamic contact angle studies of the effects of partial denaturation on immunoassay solid phase antibody.

    abstract::A range of partial denaturation antibody pre-treatments that affect immunoassay performance have been evaluated. Monoclonal anti-ferritin antibody was either partially denatured by heat, urea or pH pre-treatment or left untreated and then passively adsorbed to polystyrene microtiter wells. The adsorption characteristi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Davies J,Dawkes AC,Haymes AG,Sunderland RF,Edwards JC

    更新日期:1995-10-12 00:00:00

  • The use of T bag synthesis with paper discs as the solid phase in epitope mapping studies.

    abstract::Epitope mapping with synthetic peptides bound to derivatized paper discs has been investigated using the discs both as a solid-phase matrix for peptide synthesis as well as the solid phase in immunologic testing procedures without detachment of the peptides from the paper discs. Using the T bag (tea bag) method the si...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: van 't Hof W,van den Berg M,Aalberse RC

    更新日期:1993-05-26 00:00:00

  • A new compact and cell dense continuous culture system.

    abstract::A new compact and cell dense continuous culture system was developed. The system consisted of a culture bag, which had two compartments separated by a semipermeable membrane, and an external rotator. Cells were cultured in the inner compartment with medium containing serum or the necessary growth factors. The outer co...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Nakamura Y,Watanabe K,Noto T,Tajima T,Yamamura M

    更新日期:1989-03-10 00:00:00

  • Detection of AMP-activated protein kinase in human sera by immuno-isoelectric focusing.

    abstract::AMPKalpha is a subunit of AMP-activated protein kinase (AMPK), a heterotrimeric enzyme that works as a fuel sensor activated in response to the depletion of cellular ATP. AMPKalpha is considered as a master switch in regulating glucose and lipid metabolism. Determining its presence in patient sera may help in diagnosi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Malvoisin E,Livrozet JM,El Hajji-Ridah I,Makloufi D,Vincent N

    更新日期:2009-12-31 00:00:00

  • Determination of a glucose-containing tetrasaccharide in urine by radioimmunoassay.

    abstract::A radioimmunoassay is described that allows rapid determination of a urinary oligosaccharide -- Glc alpha 1-6Glc alpha 1-4Glc alpha 1-4Glc [(Glc)4] -- at concentrations greater than 2 pmol/microliter. Antibodies produced in rabbits immunized with the phenethylamine derivative of (Glc)4 coupled to keyhole limpet hemocy...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Zopf DA,Levinson RE,Lundblad A

    更新日期:1982-01-01 00:00:00

  • Affinity panning of peptide libraries using anti-streptokinase monoclonal antibodies: selection of an inhibitor of plasmin(ogen) active site.

    abstract::To select sequences complementary to their binding sites, two anti-streptokinase (SK) monoclonal antibodies (mAbs), A4.5 and A5.5, were used in biopanning of 15-mer and hexamer phage-displayed peptide libraries, respectively. mAb A4.5 inhibits the catalytic activity of streptokinase-plasminogen activator complex (SKPA...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Parhami-Seren B,Krudysz J,Tsantili P

    更新日期:2002-09-15 00:00:00

  • Multiplex serology of paraneoplastic antineuronal antibodies.

    abstract::Paraneoplastic neurological syndromes (PNS) are devastating neurological disorders secondary to cancer, associated with onconeural autoantibodies. Such antibodies are directed against neuronal antigens aberrantly expressed by the tumor. The detection of onconeural antibodies in a patient is extremely important in diag...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Maat P,Brouwer E,Hulsenboom E,VanDuijn M,Schreurs MW,Hooijkaas H,Smitt PA

    更新日期:2013-05-31 00:00:00

  • Rapid screening of monoclonal antibodies: new 'microstick' radioimmunoassay.

    abstract::A new system for assaying monoclonal antibodies consisting of an 8 x 12 array of sticks which fits into a 96-well microtiter plate is described. Tests using virus specific monoclonal antibodies and virus proteins demonstrated sensitivity equivalent to the conventional microtiter plate assay. Antibody production, antig...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Scheinberg DA,Pan XQ,Wilsnack R,Strand M

    更新日期:1983-03-25 00:00:00

  • A simplified immunoenzyme antigen binding technique as an approach for immunodiagnosis.

    abstract::A simple immunoenzyme technique is described in which the enzyme beta-galactosidase of E. coli (Z) is used as a marker. The principle of the test is based on the ability of the cells to bind the antigen. Conditions of the assay using Z both as the antigen and marker are described. As a screening technique, conditions ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bahr G,Modabber FZ

    更新日期:1980-01-01 00:00:00

  • Determination of mixed chimerism by a simple flow cytometry method.

    abstract::A simple, sensitive and accurate method was developed to determine the level of lymphoid chimerism in bone marrow-transplanted rodents. The method is based on flow cytometry using polyclonal alloantisera and labeled second step anti-IgG antibodies. Using mixtures of spleen cells from different mouse strains, it was de...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Leenarets PL,Vandeputte M,Waer M

    更新日期:1990-07-03 00:00:00

  • Isoelectric focusing in agarose.

    abstract::We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused. ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Saravis CA,Zamcheck N

    更新日期:1979-01-01 00:00:00

  • Simple and highly sensitive assay system for TNFR2-mediated soluble- and transmembrane-TNF activity.

    abstract::Drugs that target tumor necrosis factor-alpha (TNF) are particularly important in the treatment of severe inflammatory progression in rheumatoid arthritis, Crohn's disease and psoriasis. Despite the central role of the TNF/TNF receptor (TNFR) in various disease states, there is a paucity of information concerning TNFR...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Abe Y,Yoshikawa T,Kamada H,Shibata H,Nomura T,Minowa K,Kayamuro H,Katayama K,Miyoshi H,Mukai Y,Yoshioka Y,Nakagawa S,Tsunoda S,Tsutsumi Y

    更新日期:2008-06-01 00:00:00

  • Visualization of minute centers of viral infection in unfixed cell cultures by an enzyme-linked antibody assay.

    abstract::Enzyme-linked antibody was used to treat unfixed herpesvirus-infected human fetal lung cell cultures in a mode which permitted the visualizing of local sites of infection. Foci containing as few as 20 herpesvirus-infected cells produced sufficient viral mass to be easily detectable by this method. 'Clouds' or 'plumes'...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Smith KO,Kennell WL,Lamm DL

    更新日期:1981-01-01 00:00:00

  • A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen(s) and antibodies in tissues and fluids.

    abstract::A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein previously found to have a strong H-D a...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Mukuria JC,Naiki M,Hashimoto M,Nishiura K,Okabe M,Kato S

    更新日期:1985-06-12 00:00:00

  • Purification of a 92 kDa human immunostimulating glycoprotein obtained from the Tamm-Horsfall glycoprotein.

    abstract::A purification method for a human urinary glycoprotein (HGP92) dissociated from Tamm Horsfall protein (THP) is described. Tamm-Horsfall protein, obtained by salt precipitations, was again precipitated in presence of monovalent ions. In these conditions, Tamm-Horsfall protein displayed a tendency to form a gel. After u...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Fontan E,Jusforgues-Saklani H,Briend E,Fauve RM

    更新日期:1995-11-16 00:00:00

  • An improved semi-quantitative enzyme immunostaining procedure for glycosphingolipid antigens on high performance thin layer chromatograms.

    abstract::An immunoassay is described which allows the detection of glycosphingolipid (GSL) antigens on high performance thin layer chromatograms (HPTLC). The method involves: (1) the separation of GSL on HPTLCs; (2) incubation with specific antibodies against carbohydrate structures of GSL, and (3) the detection of specificall...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bethke U,Müthing J,Schauder B,Conradt P,Mühlradt PF

    更新日期:1986-05-01 00:00:00

  • Dialysis-based bioreactor systems for the production of monoclonal antibodies--alternatives to ascites production in mice.

    abstract::Two commercially available bioreactor systems, CELLine and miniPERM, were evaluated for their ability to support the production of monoclonal antibody (mAb) from a variety of murine hybridoma cell lines. Production and purity of mAbs were compared between the two systems and with mouse ascites tumour fluid generation....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Bruce MP,Boyd V,Duch C,White JR

    更新日期:2002-06-01 00:00:00

  • Rapid and simple measurement of human C5a-des-Arg level in plasma or serum using monoclonal antibodies.

    abstract::A new sandwich immunoassay method for measuring human C5a-des-Arg was developed using monoclonal antibody specifically reactive with C5a-des-Arg. Monoclonal antibodies were obtained from a panel of hybridomas produced by fusion of mouse myeloma cells, P3 X 63-AG8,653, with spleen cells from a CBF1(C57BL/6 X BALB/c) mo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Takeda J,Kinoshita T,Takata Y,Kozono H,Tanaka E,Hong K,Inoue K

    更新日期:1987-08-03 00:00:00

  • Partial purification of lymphokine activities by iso-electric focusing.

    abstract::A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing....

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Trudgett A

    更新日期:1976-01-01 00:00:00

  • A cassette vector for high-level reporter expression driven by a hybrid invariant chain promoter in transgenic mice.

    abstract::A plasmid cassette vector was designed to generate transgenic mice expressing reporter cDNAs at high levels in antigen-presenting cells under the control of the murine invariant chain (Ii) promoter. Analysis of several transgenic mice harboring a chimeric Ii cDNA placed in this vector showed that it can drive expressi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: van Santen H,Benoist C,Mathis D

    更新日期:2000-11-01 00:00:00

  • A novel method for the rapid bleeding of rats from the tail vein.

    abstract::A method for bleeding rats from the tail vein was developed. Sufficient vasodilation of the tail was accomplished by the use of a specially designed warming device. With the help of a physical restrainer, up to 5 ml of blood can be collected from the tail vein and no anaesthesia is required. This bleeding procedure is...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Shek PN,Howe SA

    更新日期:1982-09-17 00:00:00

  • Use of GapmeRs for gene expression knockdowns in human primary resting CD4+ T cells.

    abstract::Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not cause cell activat...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Abewe H,Deshmukh S,Mukim A,Beliakova-Bethell N

    更新日期:2020-01-01 00:00:00

  • Detection of guinea pig T mu and T gamma cells by a double rosette assay.

    abstract::By a rosette assay with ox red blood cells (ORBC) sensitised with rabbit IgM and IgG antibodies (EA mu and EA gamma), receptors for IgM (Fc mu-R) and IgG (Fc gamma-R) on the surface of guinea pig lymphocytes in the different lymphoid organs were demonstrated. A double rosette assay with rabbit erythrocytes (RRBC) and ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Kataoka S,Itoh K,Kurane I,Kumagai K

    更新日期:1982-01-01 00:00:00

  • Immunoperoxidase procedures to detect monoclonal antibodies against cell surface antigens. Quantitation of binding and staining of individual cells.

    abstract::An immunoperoxidase method has been developed which allows accurate and sensitive quantitation of the binding of monoclonal antibodies to cell surface antigens. Monolayers of fixed cells were prepared in wells of Terasaki micro-test plates and monoclonal antibodies bound to cell surface antigens were identified by the...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Lansdorp PM,Astaldi GC,Oosterhof F,Janssen MC,Zeijlemaker WP

    更新日期:1980-01-01 00:00:00

  • An assay to quantitate the binding of Leishmania amastigotes to macrophages.

    abstract::A leishmania amastigote radiobinding assay has been developed using organisms labeled with tritiated uracil. These labeled amastigotes resemble freshly isolated unlabeled amastigotes in metabolic activity, buoyant density, morphology, viability and their ability to transform into promastigotes. Organisms routinely inc...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Mosser DM

    更新日期:1990-07-03 00:00:00

  • Epitope mapping and affinity purification of monospecific antibodies by Escherichia coli cell surface display of gene-derived random peptide libraries.

    abstract::We report a method for the precise mapping of linear epitopes by presenting a peptide library on the surface of Escherichia coli cells. A random library of gene fragments derived from the classical swine fever virus (CSFV) envelope protein E(rns) was generated by DNAse I cleavage and cloned into a specially designed b...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章


    authors: Christmann A,Wentzel A,Meyer C,Meyers G,Kolmar H

    更新日期:2001-11-01 00:00:00