Effect of alpha-receptor stimulation on Cl transport by rat submandibular acini.


:Dispersed salivary acini isolated from the rat submandibular gland by enzymatic digestion were used to study the effects of alpha-receptor stimulation on transmembrane transport of 36Cl. In the absence of secretagogue, the tracer accumulated in the cells in a time-dependent manner until a steady-state content of 6.8 +/- 0.1 nmol/mg protein was attained after 3-5 min of incubation. Epinephrine (1 mumol/L) alone did not modify 36Cl accumulation but in the presence of the beta-receptor blocker propranolol (1 mumol/L) caused a significant (21%) reduction in the isotope content of the cells to 5.2 +/- 0.1 nmol/mg protein. In acini pre-loaded with 36Cl for 12 min, 1 mumol/L epinephrine caused a rapid but transient net efflux of tracer, but the isotope content subsequently increased to pre-stimulation levels. In the presence of propranolol, however, the efflux of 36Cl induced by epinephrine was larger and more sustained and was partially inhibited by the K-channel blocker quinidine (1 mmol/L) and significantly by the absence of Ca2+ in the incubation medium. The alpha-agonist phenylephrine (10 mumol/L) also significantly reduced the steady-state 36Cl content of tracer-pre-loaded cells. By contrast, exposure of the acini to epinephrine in the presence of the alpha-receptor blocker phentolamine, or the beta-agonist isoproterenol, increased the tracer content of the cells, whether the drugs were added at time zero or to tracer-pre-loaded cells.(ABSTRACT TRUNCATED AT 250 WORDS)


J Dent Res


Martinez JR,Reed P




Has Abstract


1988-03-01 00:00:00












  • Fibroblastic subpopulations in uninjured and wounded rabbit oral mucosa.

    abstract::Fibroblast cultures derived from uninjured and reparative rabbit buccal mucosa were compared in terms of extracellular glycosaminoglycan (GAG) content and cellular response to interleukin-1 (IL-1). Under identical growth conditions, proliferation of both cell lines was the same. Both lines incorporated [3H]-glucosamin...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Bronson RE,Treat JA,Bertolami CN

    更新日期:1989-01-01 00:00:00

  • COMT Diplotype Amplifies Effect of Stress on Risk of Temporomandibular Pain.

    abstract::When measured once, psychological stress predicts development of painful temporomandibular disorder (TMD). However, a single measurement fails to characterize the dynamic nature of stress over time. Moreover, effects of stress on pain likely vary according to biological susceptibility. We hypothesized that temporal es...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Slade GD,Sanders AE,Ohrbach R,Bair E,Maixner W,Greenspan JD,Fillingim RB,Smith S,Diatchenko L

    更新日期:2015-09-01 00:00:00

  • Bioengineered teeth from cultured rat tooth bud cells.

    abstract::The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for to...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Duailibi MT,Duailibi SE,Young CS,Bartlett JD,Vacanti JP,Yelick PC

    更新日期:2004-07-01 00:00:00

  • Glass-ionomer cement formulations. II. The synthesis of novel polycarobxylic acids.

    abstract::The synthesis of many polycarboxylic acids is reported. An account is given of their stability in aqueous solution and the properties of cements formed by their reaction with ion-leachable glasses. A copolymer of acrylic and itaconic acids was found to combine several favorable characteristics. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Crisp S,Kent BE,Lewis BG,Ferner AJ,Wilson AD

    更新日期:1980-06-01 00:00:00

  • Epithelial integrins with special reference to oral epithelia.

    abstract::Adhesion of epithelium to the extracellular matrix is crucial for the maintenance of systemic and oral health. In the oral cavity, teeth or artificial dental implants penetrate the soft tissue of the gingiva. In this interface, gingival soft tissue needs to be well attached via the epithelial seal to the tooth or impl...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Larjava H,Koivisto L,Häkkinen L,Heino J

    更新日期:2011-12-01 00:00:00

  • Eugenol activates Ca(2+)-permeable currents in rat dorsal root ganglion cells.

    abstract::Although little is known about the mechanism of action of eugenol, some related compounds have been compared with capsaicin derivatives in terms of the relationship between their chemical structure and activity. To elucidate whether eugenol and capsaicin act via a common mechanism at the neuronal membrane, we investig...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Ohkubo T,Kitamura K

    更新日期:1997-11-01 00:00:00

  • Role of the activation of the nuclear enzyme poly(ADP-ribose) polymerase in the pathogenesis of periodontitis.

    abstract::We have investigated the role of the activation of nuclear poly(ADP-ribose) polymerase (PARP) enzyme, a mediator of downstream nitric oxide toxicity, using a combined approach of pharmacological inhibition and genetic disruption in a ligature-induced-periodontitis model in rats and mice. Immunohistochemical analysis r...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Lohinai Z,Mabley JG,Fehér E,Marton A,Komjáti K,Szabó C

    更新日期:2003-12-01 00:00:00

  • Differential Expression and Roles of Secreted Frizzled-Related Protein 5 and the Wingless Homolog Wnt5a in Periodontitis.

    abstract::The Wingless/integrase-1 (Wnt) family of protein ligands and their functional antagonists, secreted frizzled-related proteins (sFRPs), regulate various biological processes ranging from embryonic development to immunity and inflammation. Wnt5a and sFRP5 comprise a typical ligand/antagonist pair, and the former molecul...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Maekawa T,Kulwattanaporn P,Hosur K,Domon H,Oda M,Terao Y,Maeda T,Hajishengallis G

    更新日期:2017-05-01 00:00:00

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    abstract::Modulation of the phosphoinositide signal transduction pathway by arachidonic acid (AA) in collagenase-dispersed rat submandibular acinar cells was investigated. The muscarinic agonist, carbachol, stimulated PIP2 hydrolysis and the generation of IP3 to five-fold the control levels. This response was inhibited by 75% o...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Chung HC,Fleming N

    更新日期:1992-08-01 00:00:00

  • Stem Cell-Soluble Signals Enhance Multilumen Formation in SMG Cell Clusters.

    abstract::Saliva plays a major role in maintaining oral health. Patients with salivary hypofunction exhibit difficulty in chewing and swallowing foods, tooth decay, periodontal disease, and microbial infections. At this time, treatments for hyposalivation are limited to medications (e.g., muscarinic receptor agonists: pilocarpi...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Maruyama CL,Leigh NJ,Nelson JW,McCall AD,Mellas RE,Lei P,Andreadis ST,Baker OJ

    更新日期:2015-11-01 00:00:00

  • Analysis of tempering stresses in metal-ceramic disks.

    abstract::Previous studies showed that residual compressive stresses induced by thermal tempering retarded the growth of surface cracks in bilayered porcelain disks. The objectives of the present study were: (1) to determine whether thermal tempering by air blasting reduces the length of cracks induced by microhardness indentat...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: DeHoff PH,Anusavice KJ,Vontivillu SB

    更新日期:1996-02-01 00:00:00

  • Transcriptome Reveals Cathepsin K in Periodontal Ligament Differentiation.

    abstract::Periodontal ligaments (PDLs) play an important role in remodeling the alveolar bond and cementum. Characterization of the periodontal tissue transcriptome remains incomplete, and an improved understanding of PDL features could aid in developing new regenerative therapies. Here, we aimed to generate and analyze a large...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Yamada S,Ozaki N,Tsushima K,Yamaba S,Fujihara C,Awata T,Sakashita H,Kajikawa T,Kitagaki J,Yamashita M,Yanagita M,Murakami S

    更新日期:2016-08-01 00:00:00

  • Establishment of an improved implantation technique for hamster mucous membrane irritation testing.

    abstract::Biological evaluation procedures for the acceptance of new dental materials include testing for mucous membrane irritation and inflammation, with the hamster cheek pouch used as a model system. Previous reports have indicated both a low 14-day implant retention rate and poor differentiation between positive and negati...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Roy M,Wishe HI

    更新日期:1986-11-01 00:00:00

  • Effect of hydrogen sulfide and methyl mercaptan on the permeability of oral mucosa.

    abstract::Hydrogen sulfide (H2S) and methyl mercaptan (CH3SH) are the volatile sulfur compounds (VSC) that were investigated for a possible role in the etiology of periodontal disease. The results show that the permeability of porcine non-keratinized sublingual mucosa is increased by up to 75% or 103% following exposure to H2S ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Ng W,Tonzetich J

    更新日期:1984-07-01 00:00:00

  • Localization of palatal area in human somatosensory cortex.

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    journal_title:Journal of dental research

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    authors: Bessho H,Shibukawa Y,Shintani M,Yajima Y,Suzuki T,Shibahara T

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  • Headache and co-morbid pains associated with TMD pain in adolescents.

    abstract::This case-control study evaluated the association of headache and other co-morbid pain with temporomandibular disorder (TMD) pain in adolescents and explored the temporal co-variance of headache and TMD pain. In a population-based sample of 12- to 19-year-olds, 350 patients with self-reported TMD pain and 350 healthy ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Nilsson IM,List T,Drangsholt M

    更新日期:2013-09-01 00:00:00

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    abstract::IL-18 is a pro-inflammatory cytokine that is important in the regulation of T-cells and is elevated in inflammatory disorders such as periodontal disease. Vasoactive intestinal peptide (VIP) modulates immune responses to the periodontal pathogen Porphyromonas gingivalis (Pg). Our objective was to investigate the effec...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Foster N,Andreadou K,Jamieson L,Preshaw PM,Taylor JJ

    更新日期:2007-09-01 00:00:00

  • The chemical composition of tooth enamel in recessive dystrophic epidermolysis bullosa: significance with respect to dental caries.

    abstract::Previous reports have linked the prevalence of tooth abnormalities with high caries experience in the different types of epidermolysis bullosa (EB). However, it is not known to what extent the apparent susceptibility to enamel caries is due to disease-related altered enamel chemistry in these cases. The aim of this st...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Kirkham J,Robinson C,Strafford SM,Shore RC,Bonass WA,Brookes SJ,Wright JT

    更新日期:1996-09-01 00:00:00

  • Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells.

    abstract::In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability to reconstruct dentin-pulp complexes and can result in pulpectomy because of their low potentials to accelerate dental pulp cell activities, such as migration, proliferation, and dif...

    journal_title:Journal of dental research

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    authors: Yoshida S,Wada N,Hasegawa D,Miyaji H,Mitarai H,Tomokiyo A,Hamano S,Maeda H

    更新日期:2016-10-01 00:00:00

  • Human and macaque mastication: a quantitative study.

    abstract::Significant differences exist between human and Macaca fascicularis patterns of mandibular movement during mastication. Macaque patterns display less asymmetry, more uniformity, and limited lateral excursions when compared to humans for mastication of the same food. Different anatomical structures between the two spec...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Byrd KE,Milberg DJ,Luschei ES

    更新日期:1978-07-01 00:00:00

  • Biological mechanisms of fluorosis and level and timing of systemic exposure to fluoride with respect to fluorosis.

    abstract::Enamel fluorosis can occur following either an acute or chronic exposure to fluoride during tooth formation. Fluorosed enamel is characterized by a retention of amelogenins in the early-maturation stage, and by the formation of a more porous enamel with a subsurface hypomineralization. The mechanisms by which fluoride...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: DenBesten PK,Thariani H

    更新日期:1992-05-01 00:00:00

  • Molecular and cellular mechanisms that lead to Candida biofilm formation.

    abstract::Fungal infections in the oral cavity are mainly caused by C. albicans, but other Candida species are also frequently identified. They are increasing in prevalence, especially in denture-wearers and aging people, and may lead to invasive infections, which have a high mortality rate. Attachment to mucosal tissues and to...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: ten Cate JM,Klis FM,Pereira-Cenci T,Crielaard W,de Groot PW

    更新日期:2009-02-01 00:00:00

  • Functional characteristics of atrophic parotid acinar cells from rats after liquid feeding.

    abstract::Rat parotid atrophy, induced by liquid feeding over 10 days, was manifested as gland weight loss (40%) and histologically as acinar shrinkage. Acinar secretory function was investigated in the same glands using enzymatically dispersed cell preparations and superfused gland slices. Results were normalized for the acina...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Scott J,Gunn DL

    更新日期:1994-06-01 00:00:00

  • A new ion-coating surface treatment of alloys for dental adhesive resins.

    abstract::4-META and new phosphate-methacrylate resins adhere strongly to dental alloys. However, for strengthening the water durability of the adhesive interface, the oxidation of the alloy surface is indispensable. A new oxidation method using ion-sputtering was developed, and the effectiveness of this surface treatment on tw...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Tanaka T,Hirano M,Kawahara M,Matsumura H,Atsuta M

    更新日期:1988-11-01 00:00:00

  • Antibodies against mycobacterial antigens in the synovial fluid of patients with temporomandibular disorders.

    abstract::In the absence of active pulmonary disease, mycobacterial infection frequently causes arthritis and can be considered to initiate autoimmune diseases such as rheumatoid arthritis. Temporomandibular disorder (TMD) is a disease in which pain and impaired mandibular movement appear to arise directly from degenerative or ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Adachi N,Matsumoto S,Tokuhisa M,Kobayashi K,Yamada T

    更新日期:2000-10-01 00:00:00

  • In vivo and in vitro study of 90Sr in developing rat molar enamel.

    abstract::The uptake patterns of 90Sr in developing rat molar enamel were studied in vivo and in vitro. Autoradiographic methods were used that preclude loss or translocation of tracers associated with water-soluble compounds in the sections. In eight-day-old rats injected with the tracer, 90Sr uptake in the enamel was signific...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: White BA,Deaton TG,Bawden JW

    更新日期:1980-12-01 00:00:00

  • Short-chain carboxylic acid concentration in human gingival crevicular fluid.

    abstract::Short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. It is of no small consequence, therefore, that 1- to 5-mM concentrations of these acids exhibit significant biological activity, including the a...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Niederman R,Buyle-Bodin Y,Lu BY,Robinson P,Naleway C

    更新日期:1997-01-01 00:00:00

  • Serum antibody to Porphyromonas gingivalis in chronic kidney disease.

    abstract::Potentially significant associations between periodontal disease and chronic kidney disease (CKD) have been reported in recent studies. The aim of this cross-sectional study was to investigate the association between serum antibody to the periodontal pathogen Porphyromonas gingivalis (P. gingivalis) and CKD in 215 Jap...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Iwasaki M,Taylor GW,Manz MC,Kaneko N,Imai S,Yoshihara A,Miyazaki H

    更新日期:2012-09-01 00:00:00

  • The influence of tonic muscle activation on human jaw displacement tremor.

    abstract::Jaw displacement tremor was investigated. Both tremor amplitude and tremor frequency were found to increase with increasing muscle activation. Co-contraction of jaw elevator and jaw depressor muscles was employed in order to vary muscle activation levels without the teeth being in occlusion. Reproducibility of tremor ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: McCarroll RS,De Vries JH

    更新日期:1988-08-01 00:00:00

  • Adherence of Streptococcus sanguis to salivary mucin bound to glass.

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    pub_type: 杂志文章


    authors: Stinson MW,Levine MJ,Cavese JM,Prakobphol A,Murray PA,Tabak LA,Reddy MS

    更新日期:1982-12-01 00:00:00