Abstract:
:A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern--that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Pulford KA,Erber WN,Crick JA,Olsson I,Micklem KJ,Gatter KC,Mason DYdoi
10.1136/jcp.41.8.853subject
Has Abstractpub_date
1988-08-01 00:00:00pages
853-60issue
8eissn
0021-9746issn
1472-4146journal_volume
41pub_type
杂志文章abstract::Severe osteomalacia (confirmed by the examination of thin undecalcified bone biopsy sections) associated with hypophosphataemia developed in a 60 year old woman. A skeletal x-ray survey showed a lytic lesion in the right proximal femur, and this was curetted, showing a vascular tumour. The patient's symptoms improved ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.4.446
更新日期:1987-04-01 00:00:00
abstract::Levels of the serum complement components, C3 and C4, in patients with Crohn's disease, ulcerative colitis, and miscellaneous gastrointestinal disorders were compared with those of normal blood donors. Significant increases of both components were found in all three patient groups, the highest being in patients with C...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.8.798
更新日期:1979-08-01 00:00:00
abstract::A total of 104 clinical isolates of Pr. mirabilis from three hospitals were screened for their sensitivity to chlorhexidine. The minimum inhibitory concentrations of the antiseptic for these strains ranged from 10 to 800 mug/ml. Two strains sensitive to 20 mug of chlorhexidine/ml were adapted to resistance by growth i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.4.284
更新日期:1974-04-01 00:00:00
abstract::The PAS and acid phosphatase reactions showed a different pattern of positivity in the cells of lymphoproliferative disorders according to their B or T cell nature. In B-cell leukaemias (chronic lymphocytic and prolymphocytic) a low proportion of lymphocytes gave a positive result with the acid phosphatase reaction, w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.10.767
更新日期:1974-10-01 00:00:00
abstract::A case is described in which marked hepatic fibrosis probably resulted from the prolonged treatment of acute leukaemia with methotrexate. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.1.81
更新日期:1966-01-01 00:00:00
abstract::Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and high-throughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TM...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2012-201091
更新日期:2013-03-01 00:00:00
abstract::An enzyme linked immunosorbent assay (ELISA) based on the antibody class capture method for the detection of specific IgM against Toxoplasma gondii, using the microtitre plate format, was developed. Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase. Prior mixing of th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.3.276
更新日期:1987-03-01 00:00:00
abstract::The immunofluorescent examination of nasopharyngeal secretions has been compared with conventional cell culture for the diagnosis of respiratory syncytial (RS) virus infection in children under the age of 2 years in Newcastle upon Tyne and Manchester. Two hundred and sixty-eight identifications of RS virus were made b...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.4.308
更新日期:1971-05-01 00:00:00
abstract::The potencies of six commercially manufactured heparins have been measured by the British Pharmacopoeial (BP) assay and activated partial thromboplastin time (APTT), protamine sulphate, and anti-Xa assays. The APTT/BP potency ratios were found to vary with the preparation but this was not dependent on the tissue sourc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.6.562
更新日期:1980-06-01 00:00:00
abstract::A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classificatio...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.9.831
更新日期:1971-12-01 00:00:00
abstract:AIMS:To establish the number of histological levels necessary for the evaluation of breast needle core biopsy (NCB) specimens taken from areas of mammographic calcification in patients presenting via the UK National Health Service Breast Screening Programme. METHODS:Retrospective review of a series of breast NCB speci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038190
更新日期:2007-06-01 00:00:00
abstract::Patients with bacterial infection may show altered membrane permeability of the primary azurophilic lysosomes of blood neutrophils. A new enzyme cytochemical technique, sensitive to increased membrane permeability caused by contact of neutrophils with acetone, saponin, low pH, Streptolysin O, bacteria, and nylon wool,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.7.648
更新日期:1978-07-01 00:00:00
abstract::During the last decade, a dramatic rise in the development and application of artificial intelligence (AI) tools for use in pathology services has occurred. This trend is often expected to continue and reshape the field of pathology in the coming years. The deployment of computational pathology and applications of AI ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2020-206908
更新日期:2020-08-06 00:00:00
abstract::In two out of three cases of idiopathic fibrosis of the mediastinum there was also retroperitoneal fibrosis, and in one of them extensive fibrosis of the lungs. One of these, a fit middle-aged man, died suddenly with severe coronary artery stenosis due to fibrosis. The third case was that of a young man with a solitar...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.1.78
更新日期:1978-01-01 00:00:00
abstract::The indirect immunofluorescence technique was used to study the expression of the blood group A and H antigens in fresh-frozen and formalin-fixed paraffin-embedded sections of the bladder mucosa of 21 patients with non-neoplastic diseases of the bladder. Reliable assessment of these antigens could only be made using c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.8.873
更新日期:1983-08-01 00:00:00
abstract:AIM:To determine the reliability of international normalised ratio (INR) measurement in primary care by practice nurses using near patient testing (NPT), in comparison with results obtained within hospital laboratories by varied methods. METHODS:As part of an MRC funded study into primary care oral anticoagulation man...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.7.494
更新日期:1999-07-01 00:00:00
abstract:AIMS:To identify the number and geographical distribution of post mortem examinations performed on HIV infected cadavers; to identify the areas that perform post mortem examinations so that, by invitation and discussion, a national post mortem network could be initiated. METHODS:A nationwide survey of Royal College of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.12.1076
更新日期:1993-12-01 00:00:00
abstract::Meningitis due to Gemella haemolysans developed in a 73 year old woman after thermolysis of the Gasserian ganglion for trigeminal neuralgia. The taxonomy of this organism is discussed, and previous cases of infection are reviewed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.5.558
更新日期:1985-05-01 00:00:00
abstract::The biochemical classification of the Klebsiella proposed by Cowan, Steel, Shaw, and Duguid (1960) has been applied to 50 strains isolated as the pure or predominant growth from sputum, and Klebsiella species have been correlated with the severity of the chest infection in the patient. A modification in nomenclature i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.5.478
更新日期:1962-09-01 00:00:00
abstract:OBJECTIVE:To study the expression of nuclear beta-catenin and Ki-67 in patients with normal gestation products (NGP), complete hydatidiform moles (CHM), and choriocarcinoma to elucidate their roles in carcinogenesis and their interrelations. METHODS:Expression of nuclear beta-catenin and Ki-67 was studied by immunohis...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026666
更新日期:2006-04-01 00:00:00
abstract:AIMS:To evaluate the IRIS flow microscope for the examination of urine specimens and to compare its performance with that of manual methods; in addition, to assess its usefulness as a screening system for the detection of significant bacteriuria in a routine clinical laboratory. METHODS:The IRIS system was compared wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.11.844
更新日期:2003-11-01 00:00:00
abstract::A case of idiopathic acquired sideroblastic anaemia transforming to acute myelofibrosis is reported. The appearance of atypical megakaryocytic proliferation in idiopathic acquired sideroblastic anaemia may presage the development of an acute myelofibrotic phase of this usually chronic disease. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.9.1032
更新日期:1984-09-01 00:00:00
abstract::A case of cryptococcal meningo-encephalitis is described. Treatment with the fungicidal agent amphotericin B resulted in temporary improvement, but the patient relapsed and died 17 months after diagnosis. The pathology and treatment of the disease are discussed. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.3.296
更新日期:1965-05-01 00:00:00
abstract::Two cases of peritoneal granulomatous reactions to food starch are described. They followed bowel perforation and clinically mimicked tuberculous and glove-powder starch peritonitis. Their histological differences from corn-starch peritonitis warrant attention in the absence of previous documentation of starch as a co...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.4.435
更新日期:1983-04-01 00:00:00
abstract:BACKGROUND:The "classical" antineutrophil cytoplasmic antibody (C-ANCA) pattern seen on indirect immunofluorescence (IIF) is characterised by granular cytoplasmic staining showing central or interlobular accentuation, and is strongly associated with antiproteinase-3 antibodies (PR3-ANCA) and Wegener's granulomatosis. H...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.52.2.124
更新日期:1999-02-01 00:00:00
abstract:AIMS:To study the pathogenicity and virulence characteristics of Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus sapro-phyticus. METHODS:BALB/c mice were challenged intraperitoneally with graded doses of three strains belonging to each species. LD50s were measured for each strain. Haemolysi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.8.743
更新日期:1994-08-01 00:00:00
abstract:AIMS:To investigate the histopathological features of the synovial lining of the knee following implantation of an artificial cruciate ligament. METHODS:Eighty two patients underwent anterior cruciate ligament reconstruction for chronic, symptomatic instability of the knee. The cruciate ligament was reconstructed with...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.6.556
更新日期:1995-06-01 00:00:00
abstract::One hundred and two bone marrow samples were analysed by histological and immunohistochemical methods for neurone specific enolase (NSE). The biopsies were performed to determine the extent of bone marrow disease in 84 neuroblastomas, nine embryonal rhabdomyosarcomas, five Ewing's sarcomas, two cases of Hodgkin's dise...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.4.309
更新日期:1991-04-01 00:00:00
abstract:INTRODUCTION/METHODS:After the recognition of contaminating algae on histopathological sections stained by periodic acid Schiff (PAS) and Grocott methods, a detailed audit was undertaken to assess the extent of contamination and its possible source. RESULTS:The contaminating organism was a member of the staurastrum ge...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026187
更新日期:2005-10-01 00:00:00
abstract::Gastrointestinal cell proliferation plays an important role in the maintenance of the integrity of the gastrointestinal system. The study of gastrointestinal proliferation kinetics allows a better understanding of the complexity of the system, and also has important implications for the study of gastrointestinal carci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.52.5.321
更新日期:1999-05-01 00:00:00