Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.


:A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern--that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.


J Clin Pathol


Pulford KA,Erber WN,Crick JA,Olsson I,Micklem KJ,Gatter KC,Mason DY




Has Abstract


1988-08-01 00:00:00












  • Cathepsin D detected by automated and quantitative immunohistochemistry in breast carcinomas: correlation with overall and disease free survival.

    abstract:AIM:To determine the prognostic significance and clinical relevance of cathepsin D detected by immunocytochemical assays (ICAs) in breast carcinomas. METHODS:151 patients presenting with palpable or impalpable breast carcinomas and who had not received any kind of adjuvant chemotherapy or endocrine therapy who were op...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Charpin C,Garcia S,Bouvier C,Martini F,Lavaut M,Allasia C,Bonnier P,Andrac L

    更新日期:1997-07-01 00:00:00

  • Cyclin dependent kinase inhibitor p27Kip1 expression in normal and neoplastic cervical epithelium.

    abstract:AIM:To investigate whether there is loss of the p27Kip1 protein in developing cervical cancer and whether p27Kip1 immunoreactivity has any relation to the proliferative indicator Ki-67. METHODS:The expression of p27Kip1 and Ki-67 was assessed by immunohistochemistry in serial sections from normal epithelium (13), low ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Troncone G,Vetrani A,de Rosa G,Gerbasio D,Palombini L

    更新日期:1999-12-01 00:00:00

  • Laboratory diagnosis of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.

    abstract::A simple laboratory method for culture of continuous ambulatory peritoneal dialysis (CAPD) fluids is described. Guidelines for antimicrobial therapy are discussed based on results from 18 patients studied over an 11-week period. Cephalosporins appeared to be a rational choice for therapy while awaiting laboratory resu...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Fenton P

    更新日期:1982-11-01 00:00:00

  • Biochemical assessment of acute myocardial ischaemia.

    abstract:AIMS:To evaluate the efficacy of biochemical parameters in different fluids in the diagnosis of myocardial infarction of different causes, analysed after death. METHODS:The myoglobin concentration and total creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) activities were measured in serum, pericardial flu...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Perez-Cárceles MD,Osuna E,Vieira DN,Martínez A,Luna A

    更新日期:1995-02-01 00:00:00

  • Reduced concentrations of selenium in mild Crohn's disease.

    abstract::The concentrations of selenium, zinc, and copper were determined in whole blood, plasma, and leucocytes in 20 patients with relatively mild Crohn's disease, nine of whom were being treated with steroids, and compared with those of a control group. There was a significant decrease in the concentration of selenium in th...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Hinks LJ,Inwards KD,Lloyd B,Clayton B

    更新日期:1988-02-01 00:00:00

  • Enzyme histochemical analysis on cryostat sections of human bone marrow.

    abstract::A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or d...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Chilosi M,Pizzolo G,Janossy G,Bofill M,Fiore-Donati L

    更新日期:1982-11-01 00:00:00

  • Radioimmunoassay that measures serum vitamin B12.

    abstract:AIMS:To develop a specific radioimmunoassay for the routine determination of serum vitamin B12. METHODS:Antisera were raised in rabbits by immunisation with the monocarboxylic acid derivative of cyanocobalamin coupled to human serum albumin. Antibody titres and affinities were determined and the antiserum giving the h...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: O'Sullivan JJ,Leeming RJ,Lynch SS,Pollock A

    更新日期:1992-04-01 00:00:00

  • Monoclonal antibody to cytokeratin for use in routine histopathology.

    abstract::CAM 5.2 is a murine monoclonal antibody, raised against the colon carcinoma cell line HT29, which recognises lower molecular weight intracellular cytokeratin proteins within secretory epithelia. Extensive indirect immunohistochemical studies have confirmed that this antibody stains formalin fixed (and freshly frozen) ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Makin CA,Bobrow LG,Bodmer WF

    更新日期:1984-09-01 00:00:00

  • HER2 testing in the UK: further update to recommendations.

    abstract::These guidelines update the previous UK HER2 testing guidelines and have been formulated to give advice on methodology, interpretation and quality assurance to ensure that HER2 testing results are accurate, reliable and timely with the expansion of testing to all patients with breast cancer at the time of primary diag...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审


    authors: Walker RA,Bartlett JM,Dowsett M,Ellis IO,Hanby AM,Jasani B,Miller K,Pinder SE

    更新日期:2008-07-01 00:00:00

  • Low grade marginal zone B cell lymphoma of the breast associated with localised amyloidosis and corpora amylacea in a woman with long standing primary Sjögren's syndrome.

    abstract::Primary low grade marginal zone B cell lymphoma (MZL) of the breast and localised mammary amyloidosis are exceedingly rare entities. This report describes the case of a woman with long standing Sjögren's syndrome presenting with asymptomatic MZL of the breast showing plasmacytic differentiation, associated with local ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Kambouchner M,Godmer P,Guillevin L,Raphaël M,Droz D,Martin A

    更新日期:2003-01-01 00:00:00

  • A serodiagnostic test for tuberculosis.

    abstract::This study describes a simple whole cell agglutination test for tuberculosis in which phenol-killed Mycobacterium tuberculosis H37Ra is used as the antigen. The test gave positive results in 59 of 63 culture-positive cases of tuberculosis due to M. tuberculosis, and in 4 of 11 culture-positive cases due to other mycob...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Nicholls AC

    更新日期:1975-11-01 00:00:00

  • Adult alpha1-antitrypsin deficiency.

    abstract::Three adults with alpha 1-antitrypsin deficiency are described. In two of the cases the deficiency was genetically determined (cases 1 and 2), and each demonstrated unusual features of the disease. The liver in case 1 (homozygous) showed cholangiolar hyperplasia which has been recorded only once before. Case 2 (hetero...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Sweeney EC

    更新日期:1975-08-01 00:00:00

  • Comparative genomic hybridisation in malignant deciduoid mesothelioma.

    abstract:BACKGROUND:Malignant deciduoid mesothelioma is a rare variant of epithelioid mesothelioma. This tumour generally has poor prognosis, and can be asbestos related. AIM:To identify peculiar genetic changes responsible for critical phases in pathogenesis of malignant deciduoid mesothelioma and their prognostic relevance. ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Scattone A,Pennella A,Gentile M,Musti M,Nazzaro P,Buonadonna AL,Marzullo A,Cavone D,Pollice L,Serio G

    更新日期:2006-07-01 00:00:00

  • Deletion and methylation of the tumour suppressor gene p16/CDKN2 in primary head and neck squamous cell carcinoma.

    abstract:AIMS:To study the homozygous deletion and methylation status of the 5' CpG island of the p16 and p15 genes (9p21) in a set of primary advanced head and neck squamous cell carcinomas (SCC) and to test whether inactivation of these genes by these mechanisms contributes to head and neck SCC development. METHODS:DNA was e...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: González MV,Pello MF,López-Larrea C,Suárez C,Menéndez MJ,Coto E

    更新日期:1997-06-01 00:00:00

  • Audit of an emergency biochemistry service.

    abstract:AIM:To examine a model for the evaluation of appropriateness of testing in an emergency biochemistry laboratory. METHODS:A model was devised in which incoming emergency test requests were categorised as appropriate or inappropriate. Explicit criteria were used to define eight minor categories, which were chosen to ref...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Smellie WS,Murphy MJ,Galloway PJ,Hinnie J,McIlroy J,Dryburgh FJ

    更新日期:1995-12-01 00:00:00

  • Value of ezrin, maspin and nm23-H1 protein expressions in predicting outcome of patients with head and neck squamous-cell carcinoma treated with radical radiotherapy.

    abstract:BACKGROUND:Prognostic factors in predicting outcomes in patients with head and neck squamous-cell carcinoma (HNSCC) are limited to the clinical-pathological parameters, including lymph node metastasis, location, grade and stage of the disease. AIM:To determine whether the expression of these proteins has a value in pr...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Mhawech-Fauceglia P,Dulguerov P,Beck A,Bonet M,Allal AS

    更新日期:2007-02-01 00:00:00

  • Quality of teaching in chemical pathology: ability of interns to order and interpret laboratory tests.

    abstract:BACKGROUND AND OBJECTIVE:There has been a steady decline in the overt teaching of many basic and pathology sciences in the medical curriculum worldwide. As interns are the doctors most likely to request and act on tests, an assessment of their confidence in dealing with laboratory investigations was undertaken. METHOD...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,多中心研究


    authors: Stanfliet JC,Macauley J,Pillay TS

    更新日期:2009-07-01 00:00:00

  • Use of benzidine for histological demonstration of haemoglobin in human bite marks.

    abstract::Macroscopic evidence of bruising from human bite marks may be inconclusive and routine histochemical methods of showing extravasated erythrocytes can be unreliable. Leuco patent blue staining, for the presence of peroxidase, Amido black B, a tinctorial staining method for haemoglobin, Perls's reaction for ferric iron ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Allison RT,Whittaker DK

    更新日期:1990-07-01 00:00:00

  • Search for retrovirus in the chronic fatigue syndrome.

    abstract:AIM:To examine peripheral blood and skeletal muscle from patients with chronic fatigue syndrome for exogenous retrovirus. METHODS:Blood samples from 30 patients and muscle biopsy specimens of 15 patients were examined for retroviral sequences by DNA extraction, polymerase chain reaction (PCR), and Southern blotting hy...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Gow JW,Simpson K,Schliephake A,Behan WM,Morrison LJ,Cavanagh H,Rethwilm A,Behan PO

    更新日期:1992-12-01 00:00:00

  • Frequency distribution and 'reference values' of plasma alkaline phosphatase (EC activity in the adult population of a Scottish new town.

    abstract::As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Gardner MD,Scott R

    更新日期:1978-12-01 00:00:00

  • Prevalence and intraoral distribution of Candida albicans in Sjögren's syndrome.

    abstract::An imprint culture technique has been employed to study the prevalence and intraoral distribution of Candida albicans in 16 patients with Sjögren's syndrome and in 16 healthy controls matched for age, sex, and dental status. The prevalence and intraoral density of C. albicans was found to be significantly higher at al...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Tapper-Jones L,Aldred M,Walker DM

    更新日期:1980-03-01 00:00:00

  • Alpha-hydroxybutyrate dehydrogenase activity in sex-linked muscular dystrophy.

    abstract::In two families with severe sex-linked muscular dystrophy, high levels of alpha-hydroxybutyrate dehydrogenase (HBD), lactate dehydrogenase (LD), aspartate transaminase (AspT), aldolase, and creatine phosphokinase (CPK) were found in the sera of three young affected males. In both families the mother had a raised level...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Johnston HA,Wilkinson JH,Withycombe WA,Raymond S

    更新日期:1966-05-01 00:00:00

  • Rapid identification of Candida species in oral rinse solutions by PCR.

    abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Liguori G,Lucariello A,Colella G,De Luca A,Marinelli P

    更新日期:2007-09-01 00:00:00

  • Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.

    abstract::Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Grange JM

    更新日期:1978-04-01 00:00:00

  • Morphological effects of chemotherapy on ovarian carcinoma.

    abstract:AIMS:Traditionally, advanced stage ovarian carcinoma is treated by debulking surgery followed by chemotherapy. However, in some circumstances preoperative chemotherapy may be given before optimal surgical debulking. This study aims to describe the morphological features found in ovarian carcinoma after chemotherapy bec...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: McCluggage WG,Lyness RW,Atkinson RJ,Dobbs SP,Harley I,McClelland HR,Price JH

    更新日期:2002-01-01 00:00:00

  • Spherical connective tissue inclusions in epithelial hyperplasia of the breast ("collagenous spherulosis").

    abstract::Partial myoepithelial differentiation is common in simple epithelial hyperplasia (epitheliosis) of the breast but functional myoepithelial differentiation with basement membrane production is exceedingly rare. A peculiar change of hyaline globules within benign epithelial hyperplasia has been recognised before as "col...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Wells CA,Wells CW,Yeomans P,Viña M,Jordan S,d'Ardenne AJ

    更新日期:1990-11-01 00:00:00

  • Use of the 'Analmatic clinical system' in the microbiological assay of vitamin B 12 and folic acid in serum.

    abstract::The routine estimation of large numbers of serum folic acid and vitamin B(12) levels using the Baird and Tatlock(1) Analmatic clinical system is described and the method evaluated.Precision, accuracy, and carryover are shown to be at acceptable levels, and the advantages and disadvantages of the use of the system are ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Colvin G,Gibson GL,Neill DW

    更新日期:1971-02-01 00:00:00

  • Detection of campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea.

    abstract::Rabbit antiserum, elicited by the intravenous injection of a strain of Campylobacter jejuni heated to 100 degrees C, cross reacted strongly with all other thermophilic campylobacters tested as well as with "C pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. Antisera to formali...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Price AB,Dolby JM,Dunscombe PR,Stirling J

    更新日期:1984-09-01 00:00:00

  • Bilateral gonadoblastoma/dysgerminoma in a 46 XY individual: case report with hormonal studies.

    abstract::A case of bilateral gonadoblastoma/dysgerminoma in a 46 XY phenotypical female is presented. Increased circulating beta human chorionic gonadotropin (beta-HCG) and testosterone together with a decreased concentration of follicle stimulating hormone (FSH) reflected the activities of these tumours. The patient is alive ...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章


    authors: Fisher RA,Salm R,Spencer RW

    更新日期:1982-04-01 00:00:00

  • The spectrum of histopathological changes encountered in pancreatectomy specimens after neoadjuvant chemoradiation, including subtle and less-well-recognised changes.

    abstract::Preoperative (neoadjuvant) chemoradiation therapy/treatment (NCRT) is emerging as an important treatment modality in borderline resectable pancreatic ductal adenocarcinoma (PDAC). The constellation of histopathological changes secondary to chemoradiation is diverse and has been well documented, particularly in other g...

    journal_title:Journal of clinical pathology

    pub_type: 杂志文章,评审


    authors: Kalimuthu SN,Serra S,Dhani N,Chetty R

    更新日期:2016-06-01 00:00:00