Abstract:
BACKGROUND:Helicobacter pylori infection is associated with risk for chronic gastritis (CG), gastric ulcer (GU), duodenal ulcer (DU), and gastric cancer (GC). The H. pylori Cag type IV secretion system (TFSS) translocates the virulence factor cytotoxin-associated gene A protein into host cells and plays an important role in initiating gastric carcinogenesis. The CagL and CagI proteins are components of the TFSS. The Arg-Gly-Asp (RGD) motif of CagL, and the six most distal C-terminal amino acids (Ser-Lys-Ile-Ile-Val-Lys, and Ser-Lys-Val-Ile-Val-Lys) of CagL and CagI are essential for TFSS adhesion to host cells. Additionally, the CagL variant Tyr58Glu59 was previously shown to be associated with GC patients. RESULTS:We isolated 43 H. pylori isolates from 17 CG, 8 GU, 8 DU, and 10 GC patients in Southeast Asia. Total DNAs were extracted and sequenced with MiSeq. H. pylori strain ATCC 26695, which was isolated from CG patients, was used as a reference. We examined the full sequences of H. pylori cagL and cagI using whole-genome sequencing (WGS), and analyzed whether single nucleotide variants and amino acid changes (AACs) correlated with adverse clinical outcomes. Three isolates were excluded from the analysis due to cagPAI rearrangements. CagL RGD motifs were conserved in 39 isolates (97.5%). CagL-Glu59 and Ile234 in the C-terminal motif were more common in 10 H. pylori isolates from GC patients (p < 0.001 and p < 0.05, respectively). When 5 Vietnamese isolates from GC patients were excluded, CagL-Glu59 still remains significant (p < 0.05), but not Ile234. CagL-Tyr58 was seen in only one isolate. The CagI C-terminal motif was completely conserved across all 40 isolates, and there were no significant AACs in CagI. CONCLUSIONS:Using WGS, we analyzed genetic variants in clinical H. pylori isolates and identified putative novel and candidate variants in uncharacterized CagL and CagI sequences that are related to gastric carcinogenesis. In particular, CagL-Glu59 has the possible association with GC.
journal_name
Gut Pathogjournal_title
Gut pathogensauthors
Ogawa H,Iwamoto A,Tanahashi T,Okada R,Yamamoto K,Nishiumi S,Yoshida M,Azuma Tdoi
10.1186/s13099-017-0165-1subject
Has Abstractpub_date
2017-04-21 00:00:00pages
21issn
1757-4749pii
165journal_volume
9pub_type
杂志文章相关文献
Gut Pathogens文献大全abstract:BACKGROUND:Coeliac disease is an autoimmune disorder resulting from an interaction between diet, genome and immunity. The treatment of CoeD is lifelong adherence to a gluten free diet, which is associated with clinical and histological improvements. However, a substantive number of individuals report only partial sympt...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0173-1
更新日期:2017-05-05 00:00:00
abstract:BACKGROUND:Intestinal microorganisms affect host physiology, including ageing. Given the difficulty in controlling for human studies of the gut microbiome, mouse models provide an alternative avenue to study such relationships. In this study, we report on the complete genome of "Faecalibaculum rodentium" ALO17, a bacte...
journal_title:Gut pathogens
pub_type:
doi:10.1186/s13099-016-0087-3
更新日期:2016-02-12 00:00:00
abstract:BACKGROUND:Ruminants, in particular bovines, are the primary reservoir of Shiga toxin-producing E. coli (STEC), but whole genome analyses of the current German ESBL-producing O104:H4 outbreak strain of sequence type (ST) 678 showed this strain to be highly similar to enteroaggregative E. coli (EAEC). Strains of the EAE...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-3-17
更新日期:2011-11-03 00:00:00
abstract:Background:Campylobacter jejuni is considered as a chicken commensal. The gut microbiota and the immune status of the host may affect its colonization. Infectious bursal disease virus (IBDV) is an immunosuppressive virus of chickens, which allows secondary pathogens to invade or exacerbates their pathogenesis. To inves...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-018-0241-1
更新日期:2018-03-30 00:00:00
abstract:BACKGROUND:Shigellosis is an acute form of gastroenteritis caused by the bacteria belonging to the genus Shigella. It is the most common cause of morbidity and mortality in children. Shigella belongs to the family Enterobactericeae, which is a Gram-negative and rod shaped bacterium. In the present study, we report the ...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-6-28
更新日期:2014-07-11 00:00:00
abstract:BACKGROUND:Contamination from fecal bacteria in recreational waters is a major health concern since bacteria capable of causing human disease can be found in animal feces. The Dog Beach area of Ocean Beach in San Diego, California is a beach prone to closures due to high levels of fecal indicator bacteria (FIB). A pote...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-3-10
更新日期:2011-07-07 00:00:00
abstract:BACKGROUND:Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative ...
journal_title:Gut pathogens
pub_type:
doi:10.1186/s13099-017-0175-z
更新日期:2017-05-15 00:00:00
abstract:BACKGROUND:Campylobacter enteritis represents a risk factor for the development of inflammatory bowel disease (IBD) via unknown mechanisms. As IBD patients exhibit inflammatory responses to their commensal intestinal microflora, factors that induce translocation of commensal bacteria across the intestinal epithelium ma...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-1-2
更新日期:2009-02-03 00:00:00
abstract:BACKGROUND:Toll-like receptors (TLRs) are the major pattern recognition receptors that mediate sensing of a wide range of microorganisms. TLR2 forms heterodimers with either TLR1 or TLR6, broadening its ligand diversity against pathogens. TLR1, TLR2 and TLR6 have been implicated in the recognition of Candida albicans, ...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0158-0
更新日期:2017-02-15 00:00:00
abstract:BACKGROUND:Acid treatment is commonly used for controlling or killing pathogenic microorganisms on medical devices and environments; however, inadequate acid treatment may cause acid tolerance response (ATR) and offer cross-protection against environmental stresses, including antimicrobials. This study aimed to charact...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-020-00390-5
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:We investigated the interaction of Mycobacterium avium subspecies paratuberculosis, M. bovis and M. tuberculosis and different glial cells (enteric glial and microglial cells) in order to evaluate the infecting ability of these microorganisms and the effects produced on these cells, such as the evaluation of...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-3-19
更新日期:2011-12-09 00:00:00
abstract::In the present study we demonstrate that the initial attachment of Listeria monocytogenes cells to plastic surfaces was significantly increased by growth in the presence of bile. Improved biofilm formation was confirmed by crystal violet staining, microscopy and bioluminescence detection of a luciferase-tagged strain....
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-1-11
更新日期:2009-05-28 00:00:00
abstract:Background:Enterobacter hormaechei is an important emerging pathogen and a key member of the highly diverse Enterobacter cloacae complex. E. hormaechei strains can persist and spread in nosocomial environments, and often exhibit resistance to multiple clinically important antibiotics. However, the genomic regions that ...
journal_title:Gut pathogens
pub_type:
doi:10.1186/s13099-019-0288-7
更新日期:2019-02-13 00:00:00
abstract:Background:Shigella has evolved as a result of acquiring extragenetic material through horizontal gene exchange. These aid in the rapid emergence of bacterial inter-strain diversity in virulence factors and serotype variants through O-antigenic switching. Plasmid incompatibility typing of isolates is insightful in unde...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-019-0314-9
更新日期:2019-06-14 00:00:00
abstract:BACKGROUND:The development of efficacious alternatives to antimicrobial growth promoters (AGP) in livestock production is an urgent issue, but is hampered by a lack of knowledge regarding the mode of action of AGP. The belief that AGP modulate the intestinal microbiota has become prominent in the literature; however, t...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-3-14
更新日期:2011-09-25 00:00:00
abstract:Background:The human gut microbiome has an important role in health and disease. There is extensive geographical variation in the composition of the gut microbiome, however, little is known about the gut microbiome composition of people from the Arabian Peninsula. In this study, we describe the gut microbiome of Arab K...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-020-00351-y
更新日期:2020-02-26 00:00:00
abstract:BACKGROUND:Shigella is a frequent cause of bacterial dysentery in the developing world. Treatment with effective antibiotics is recommended for shigellosis, but options become limited due to globally emerging resistance. One of the mechanisms for the development of resistance utilizes integrons. This study described th...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-2-18
更新日期:2010-12-09 00:00:00
abstract::The two-stage neuroinflammatory process, containment and progression, proposed to underlie neurodegeneration may predicate on systemic inflammation arising from the gastrointestinal tract. Helicobacter infection has been described as one switch in the pathogenic-circuitry of idiopathic parkinsonism (IP): eradication m...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-1-20
更新日期:2009-11-26 00:00:00
abstract:BACKGROUND:The human and animal intestinal tract harbors a complex community of microbes which enables bacteria to inherit antibiotic resistance genes. The aims of this study were to investigate clonality, antimicrobial resistance, prevalence and gene cassette arrays of class I and II integrons among commensal Escheric...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-016-0123-3
更新日期:2016-08-30 00:00:00
abstract:BACKGROUND:Typhoid fever has caused severe epidemics in many Asian and African countries. The early detection of outbreaks and their sources may promote the prevention and control of typhoid fever, for which effective and timely molecular subtyping techniques are required. Pulsed field gel electrophoresis (PFGE) is rou...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-016-0094-4
更新日期:2016-04-04 00:00:00
abstract:BACKGROUND:Recently, we found that the probiotic strain Bacillus coagulans GBI-30, 6086 (GanedenBC30) improved indices of Clostridium difficile (C. difficile)-induced colitis in mice (Fitzpatrick et al., Gut Pathogens, 2011). Our goal was to determine if BC30 could also prevent the recurrence of C. difficile-induced co...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-4-13
更新日期:2012-10-22 00:00:00
abstract:BACKGROUND:There are multiple etiologies responsible for infectious gastroenteritis causing acute diarrhea which are often under diagnosed. Also acute diarrhea is one of the major causes of morbidity and mortality among children less than 5 years of age. METHODS:In our study, fecal samples (n = 130) were collected fro...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0166-0
更新日期:2017-04-11 00:00:00
abstract:BACKGROUND:One of the working mechanisms of probiotic bacteria is their ability to compete with pathogens. To define the probiotic properties of seven Lactic Acid Bacteria (LAB) strains, we tested them for survival in simulated gastro-intestinal conditions, antimicrobial activities, co-aggregative abilities, and interf...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0162-4
更新日期:2017-03-06 00:00:00
abstract::Of 19 environmental Escherichia coli (n = 12) and Klebsiella pneumoniae (n = 7) tested for quinolone resistance-related genes qnrA, qnrB, qnrC, qnrS and qepA, four each of E. coli and K. pneumoniae possessed qnrS, and another E. coli isolate possessed a new variant of qepA. This is the first detection of qepA in envir...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0207-8
更新日期:2017-10-23 00:00:00
abstract:BACKGROUND:Physiological stressors may alter susceptibility of the host intestinal epithelium to infection by enteric pathogens. In the current study, cytotoxic effect, adhesion and invasion of Salmonella enterica serovar Typhimurium (S. Typhimurium) to Caco-2 cells exposed to thermal stress (41 degrees C, 1 h) was inv...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-1-14
更新日期:2009-07-09 00:00:00
abstract:Background:After an environmental disaster, the affected community is at increased risk for persistent abdominal pain but mechanisms are unclear. Therefore, our study aimed to determine association between abdominal pain and poor water, sanitation and hygiene (WaSH) practices, and if small intestinal bacterial overgrow...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0224-7
更新日期:2017-12-14 00:00:00
abstract:BACKGROUND:The putative H. pylori pathogenicity-associated factor dupA has been associated with IL-8 induction in vitro, and duodenal ulcer (DU) and gastric cancer (GC) development in certain populations, but this association is inconsistent between studies. We aimed to investigate dupA prevalence in clinical isolates ...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/1757-4749-1-5
更新日期:2009-03-11 00:00:00
abstract:Background:The root extract of Rhodiola rosea has historically been used in Europe and Asia as an adaptogen, and similar to ginseng and Shisandra, shown to display numerous health benefits in humans, such as decreasing fatigue and anxiety while improving mood, memory, and stamina. A similar extract in the Rhodiola fami...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-018-0239-8
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:Shiga toxin (Stx) producing Escherichia coli (E. coli) (STEC) is the most frequent cause of diarrhoea-positive haemolytic uraemic syndrome (D + HUS) in humans. In 2011, a huge outbreak with an STEC O104:H4 strain in Germany highlighted the limited possibilities for causative treatment of this syndrome. The r...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-017-0179-8
更新日期:2017-05-25 00:00:00
abstract:BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) is an important pathogen that causes childhood and travelers' diarrhea. Here, we present the draft genomes of four ETEC isolates recovered from stool specimens of patients with diarrhea in Beijing, China and Dhaka, Bangladesh, respectively. RESULTS:We obtained the dra...
journal_title:Gut pathogens
pub_type: 杂志文章
doi:10.1186/s13099-015-0059-z
更新日期:2015-04-08 00:00:00