Abstract:
:Backgroud/Aims: The aim of this study was to investigate the potential renoprotective effect of rapamycin on the autophagy of podocytes treated with the supernatant of mesangial cells cultured with aggregated IgA1 (aIgA1) from immunoglobulin A nephropathy (IgAN) patients. METHODS:Monomeric IgA1 (mIgA1) was isolated from the serum of IgAN patients or healthy volunteers, and then transformed to aIgA1 by heating. Subsequently, the aIgA1-mesangial cell supernatant was prepared by collecting the medium of mouse mesangial cells (MSC1097) cultured with aIgA1 (100 mg/L) from different IgAN patients or healthy volunteers for 48 h. Subsequently mouse podocytes (MPC5) were exposed to the supernatant of the aIgA1-mesangial cells for 24 h, using 100 mg/L aIgA1 from healthy volunteers as the control group or 100 mg/L aIgA1 from IgAN patients as the IgANs group, in RPMI 1640 medium. The MPC5 cells in the IgANs+Rap group were cultured with rapamycin (10 nmol/L) and the supernatant of MSC-1097 cells cultured with aIgA1 from IgAN patients in RPMI 1640 medium. Autophagy was assessed by western blot analysis (LC3, p62), electron microscopy, and immunofluorescence staining (LC3, p62, and CD63). The apoptosis of podocytes was evaluated by flow cytometry, and the expression of apoptosis-associated proteins cleaved-caspase-3 and caspase-3 were determined by western blot analysis. RESULTS:Deficient autophagy, which was evident by decreased LC3-II and CD63 levels, caused accumulation of p62, and fewer autophagosomes were observed in the MPC5 cells cultured with the IgAN supernatant, along with stronger expression of cleaved caspase-3 and a higher apoptosis rate. Inhibition of autophagy was alleviated in the IgANs+Rap group. The LC3-II/LC3-I ratio increased by almost 30%, the accumulated p62 amount was reduced by 50%, and the number of autophagosomes per podocyte increased to about 7 times that of the IgAN groups. These results were confirmed by immunofluorescence staining. In addition, the apoptosis rate of MPC5 cells decreased from 19.88% in the IgAN group to 16.78% in the IgANs+Rap group, which was accompanied by a weaker expression level of cleaved caspase-3. CONCLUSIONS:Rapamycin can reduce the apoptosis of podocytes by inducing autophagy in IgAN.
journal_name
Kidney Blood Press Resjournal_title
Kidney & blood pressure researchauthors
Liang S,Jin J,Lin B,Gong J,Li Y,He Qdoi
10.1159/000475484subject
Has Abstractpub_date
2017-01-01 00:00:00pages
177-187issue
1eissn
1420-4096issn
1423-0143pii
000475484journal_volume
42pub_type
杂志文章abstract:BACKGROUND/AIMS:Angiotensin converting enzyme 2 (ACE2) is highly expressed in the kidney and recognized to be renoprotective by degrading Angiotensin II to Angiotensin (1-7) in diabetic nephropathy. However, little is known about the role of urinary ACE2 (UACE2) in diabetes. The present study was performed to evaluate ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368486
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The biological properties of oxidized and non-oxidized PTH are substantially different. Oxidized PTH (oxPTH) loses its PTH receptor-stimulating properties, whereas non-oxidized PTH (n-oxPTH) is a full agonist of the receptor. This was described in more than 20 well published studies in the 1970(s) and 80(s)....
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350149
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:The aim of our study was to retrospectively analyse data of 520 Czech patients with IgA nephropathy (IgAN) and to specify the risk factors affecting renal survival of IgAN patients. METHODS:Cox proportional hazards regression model was used to evaluate the effects of different variables on renal surviv...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368467
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Studies have demonstrated that cholesterol variability is an independent predictor of cerebrovascular and cardiovascular events. OBJECTIVE:This study aimed to investigate the association of visit-to-visit variability in total cholesterol (TC) with kidney decline in a Chinese community-based population. MET...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000501367
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND/AIMS:Renalase (gene name RNLS), a recently discovered enzyme with monoamine oxidase activity, is implicated in the degradation of catecholamines. Recent studies indicate that common variations in the gene with RNLS are associated with hypertension. The aim of this study was to examine the association between...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000368460
更新日期:2014-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are short, single-stranded, noncoding RNAs that modulate many key biological processes by simultaneously suppressing multiple target genes. Among them, miR-26a, a conserved miRNA among vertebrates, is highly expressed in various tissues. Accumulating evidence demonstrates that miR-26a plays pivotal ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000499646
更新日期:2019-01-01 00:00:00
abstract:AIMS:To investigate whether the regulation of aquaporin (AQP) channels is altered by inhibition of type 2 11beta-hydroxysteroid dehydrogenase (11betaHSD2). METHODS:Male Sprague-Dawley rats were treated with glycyrrhizic acid (GA, 2 g/l drinking water) for 7 days. The expression of AQP2 and AQP3 was determined in the k...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000098435
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND/AIMS:To gain insight into the effect of arginine vasopressin (AVP) on renal hemodynamics in hypertensive rats, we investigated the vasoconstrictive response to AVP on total renal blood flow (RBF) and total and zonal glomerular filtration rate (GFR) in young and old spontaneously hypertensive rats (SHR). A hy...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000054225
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND/AIMS:The presence of protein-energy wasting (PEW) among dialysis patients is a crucial risk factor for outcomes. The complicated pathogenesis of PEW makes it difficult to assess and treat. This single-center retrospective study focuses on the association between nutritional markers and the outcomes of contin...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000485926
更新日期:2017-01-01 00:00:00
abstract::Cardiovascular (CV) morbidity and mortality is greatly enhanced in patients with chronic kidney disease, compared to the non-renal population. One key element of this high CV burden appears to be arterial stiffness, as an expression of premature vascular aging. Increased arterial stiffness in renal patients may be a c...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,评审
doi:10.1159/000100905
更新日期:2007-01-01 00:00:00
abstract:AIMS AND METHODS:Our aim was to evaluate the antihypertensive efficacy and tolerability of angiotensin II type 1 (AT1) receptor antagonists. Valsartan or candesartan cilexetil was administered to 11 dialysis patients with elevated blood pressure. The patients (6 male, 5 female; mean age +/- SD 61+/-11 years) were on re...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000054209
更新日期:2001-01-01 00:00:00
abstract::Currently, the pathogenesis of nondipper hypertension remains largely unclear in patients without any renal or endocrine pathology. It is well known that overt hypothyroidism is strongly associated with diastolic hypertension. However, no study has addressed the pathogenic role of TSH, free T3 (FT3), and free T4 (FT4)...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000110082
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND/AIMS:The main purpose of the present study was to determine the effect of peritoneal charge barrier dysfunction on hypoalbuminemia during CAPD. METHODS:We measured the association of dialysis dose, peritoneal equilibration test (PET) results (ratio of dialysate and plasma creatinine), and peritoneal charge ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000350150
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:We have reported that the long-term administration of angiotensin II receptor blockers (ARBs) induced unusual proliferative changes of renal afferent arteriolar smooth muscle cells (SMCs) in rats, associated with the overproduction of renin. In this study, we examined that a direct renin inhibitor (DRI:...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000443459
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:Angiotensin-converting enzyme inhibitors and angiotensin II receptor antagonists have beneficial effects on impaired fibrinolytic activity of hypertensive patients. The aim of the study was to evaluate the effect of antialdosterone treatment on impaired fibrinolysis of hypertensive patients. METHODS:Fo...
journal_title:Kidney & blood pressure research
pub_type: 临床试验,杂志文章
doi:10.1159/000066348
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND/AIMS:The metabolic syndrome (MetS), as assessed using dichotomous criteria, is associated with increased risk of future chronic kidney disease (CKD), though this relationship is unclear among African Americans, who have lower risk for MetS but higher risk for CKD. METHODS:We performed logistic regression us...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000488829
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Uremic toxins have been shown to promote glomerular hypertrophy. The present study was performed to elucidate the relation between uremic toxins and tubulointerstitial changes. METHODS:Sixty male Sprague-Dawley rats underwent 2/3 nephrectomy (Nx; n = 30) and 4/5 Nx (n = 30). Experiments were initiated 2 wee...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000080935
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Diabetic nephropathy constitutes a large proportion of end-stage kidney failure in diabetic patients. However, the underlying molecular mechanisms remain unclear. METHODS:Db/db diabetic mouse models and high glucose (HG)-induced human renal mesangial cells (HRMCs) were used as research models in vivo and in...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000508078
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND/AIMS:Either protein-to-creatinine ratio (PCR) or albumin-to-creatinine ratio (ACR) can be adopted for estimation of proteinuria in patients with chronic kidney disease (CKD). Estimated protein excretion rate (ePER) and estimated albumin excretion rate (eAER) may be superior to ACR and PCR. Reports show that ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000443429
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:Co-deposition of mannose-binding lectin (MBL) and IgG4 anti-phospholipase A2 receptor (anti-PLA2R) autoantibodies under subepithelial cells has been observed in patients with idiopathic membranous nephropathy (iMN), but the relationships of MBL deposition to iMN severity and progression remain unclear. METHO...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000508665
更新日期:2020-01-01 00:00:00
abstract::In the present study, the effects of hexachlorobenzene (HCB) on lipid peroxidation and heme metabolism in the different constitutive suborgans of the kidney were determined. For this purpose, conjugated diene and malondialdehyde levels, as lipid peroxidation parameters, and porphyrin accumulation, uroporphyrinogen dec...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000025950
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND AIM:Cinacalcet effectively reduces calcium in patients with persistent hyperparathyroidism (HPT) after kidney transplantation. We aimed to assess the association of cinacalcet with a decrease in renal function based on a meta-analysis of observational studies in kidney transplant patients with persistent...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,meta分析
doi:10.1159/000323902
更新日期:2011-01-01 00:00:00
abstract::Angiotensin-(1-7), (Ang-(1-7)), a metabolite of Ang II and /or Ang I, was infused into the renal artery (i.r.a) of anesthetized dogs in order to demonstrate its possible direct renal action. The dose administered, 15 Ig/kg BW/min in isotonic saline (0.072 ml/kg BW/min) throughout the experiment, did not influence the ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000025959
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND/AIMS:Subclinical hypovolemia may contribute to allograft dysfunction in long-term kidney transplant (KT) patients. In order to predict responsiveness to saline hydration, indices for tubular transport were investigated. METHODS:Fifty-four clinically euvolemic long-term KT patients with recently aggravated a...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000341124
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:We examined the factors associated with blood pressure variability in a multi-ethnicity prospective study in China to gain more evidence to guide the prevention and management of hypertension through risk factor intervention. METHODS:A total of 318 consecutive adult subjects aged 29-94 years with suspe...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000477424
更新日期:2017-01-01 00:00:00
abstract::The effects on glomerular hemodynamics of angiotensin-converting enzyme (ACE) inhibition with captopril and of angiotensin II receptor blockade with EXP 3174, an active metabolite of losartan, either alone or in combination, were compared in anesthetized dogs. Administration of EXP 3174 intrarenally led to an increase...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000174256
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Hypertension in the elderly is commonly characterized by an elevation of pulse pressure. With regard to advanced arteriosclerosis and limited physical fitness, doubt was casted whether elderly patients still achieve relevant cardiovascular benefits by physical exercise. The present work examines the impact o...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000104093
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Treatment with angiotensin-converting enzyme inhibitors (ACEIs) or angiotensin 2 receptor blockers (ARBs) is associated with an increased risk for acute kidney injury after cardiovascular interventions. However, for patients undergoing kidney biopsy, no data is available. METHODS:Four hundred and sixty-six ...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000339707
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIM:Our aim was to determine whether altered human β-defensin (HBD), pro-inflammatory cytokines including interleukin (IL)-6 and tumor necrotic factor (TNF)-α could increase the risk of developing and exacerbation of chronic kidney disease (CKD), especially for patients with diabetic nephropathy (DN). METHO...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000355740
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:Chronic renal allograft dysfunction (CRAD) is a leading cause of long-term renal allograft loss. Oxidative stress may account for the nonspecific interstitial fibrosis and tubular atrophy that occur in CRAD. An antioxidant intervention via Nrf2 signaling pathway activation might be a promising therapy f...
journal_title:Kidney & blood pressure research
pub_type: 杂志文章
doi:10.1159/000487501
更新日期:2018-01-01 00:00:00