当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9.

Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9.

Abstract：

:The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for gene editing in a plethora of organisms. Here, utilizing this system we developed an efficient protocol for the generation of heritable germline mutations in the parasitoid jewel wasp, Nasonia vitripennis, a rising insect model organism for the study of evolution, development of axis pattern formation, venom production, haplo-diploid sex determination, and host-symbiont interactions. To establish CRISPR-directed gene editing in N. vitripennis, we targeted a conserved eye pigmentation gene cinnabar, generating several independent heritable germline mutations in this gene. Briefly, to generate these mutants, we developed a protocol to efficiently collect N. vitripennis eggs from a parasitized flesh fly pupa, Sarcophaga bullata, inject these eggs with Cas9/guide RNA mixtures, and transfer injected eggs back into the host to continue development. We also describe a flow for screening mutants and establishing stable mutant strains through genetic crosses. Overall, our results demonstrate that the CRISPR/Cas9 system is a powerful tool for genome manipulation in N. vitripennis, with strong potential for expansion to target critical genes, thus allowing for the investigation of several important biological phenomena in this organism.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Li M,Au LYC,Douglah D,Chong A,White BJ,Ferree PM,Akbari OS

doi

10.1038/s41598-017-00990-3

subject

Has Abstract

pub_date

2017-04-19 00:00:00

pages

901

issue

1

issn

2045-2322

pii

10.1038/s41598-017-00990-3

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • The importance of common and the irrelevance of rare species for partition the variation of community matrix: implications for sampling and conservation.

    abstract：:In community ecology, it is important to understand the distribution of communities along environmental and spatial gradients. However, it is common for the residuals of models investigating those relationships to be very high (> 50%). It is believed that species' intrinsic characteristics such as rarity can contribut...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-76833-5

    authors： Brasil LS,Vieira TB,Andrade AFA,Bastos RC,Montag LFA,Juen L

    更新日期：2020-11-13 00:00:00

  • Vibrio vulnificus VvhA induces autophagy-related cell death through the lipid raft-dependent c-Src/NOX signaling pathway.

    abstract：:VvhA, a virulent factor of Vibrio (V.) vulnificus, induces acute cell death in a destructive manner. Autophagy plays an important role in cell death, but the functional role of VvhA in autophagy-related cell death has not been elucidated yet. We found that rVvhA significantly increased LC3 puncta formation and autopha...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep27080

    authors： Song EJ,Lee SJ,Lim HS,Kim JS,Jang KK,Choi SH,Han HJ

    更新日期：2016-06-02 00:00:00

  • OpenEP: an open-source simulator for electroporation-based tumor treatments.

    abstract：:Electroporation (EP), the increase of cell membrane permeability due to the application of electric pulses, is a universal phenomenon with a broad range of applications. In medicine, some of the foremost EP-based tumor treatments are electrochemotherapy (ECT), irreversible electroporation, and gene electrotransfer (GE...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79858-y

    authors： Marino M,Luján E,Mocskos E,Marshall G

    更新日期：2021-01-14 00:00:00

  • Comparative computational analysis of pluripotency in human and mouse stem cells.

    abstract：:Pluripotent cells can be subdivided into two distinct states, the naïve and the primed state, the latter being further advanced on the path of differentiation. There are substantial differences in the regulation of pluripotency between human and mouse, and in humans only stem cells that resemble the primed state in mo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07927

    authors： Ernst M,Abu Dawud R,Kurtz A,Schotta G,Taher L,Fuellen G

    更新日期：2015-01-21 00:00:00

  • Engineering preferentially-aligned nitrogen-vacancy centre ensembles in CVD grown diamond.

    abstract：:Here we report a method for improving the magnetic field sensitivity of an ensemble of Nitrogen-Vacancy (NV) centres in 12C-enriched diamond aligned along the [111] crystal axis. The preferentially-aligned NV centres are fabricated by a Plasma Enhanced Chemical Vapour Deposition (PECVD) process and their concentration...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-42314-7

    authors： Osterkamp C,Mangold M,Lang J,Balasubramanian P,Teraji T,Naydenov B,Jelezko F

    更新日期：2019-04-08 00:00:00

  • A Guide to Design Functional Molecular Liquids with Tailorable Properties using Pyrene-Fluorescence as a Probe.

    abstract：:Solvent-free, nonvolatile, room-temperature alkylated-π functional molecular liquids (FMLs) are rapidly emerging as a new generation of fluid matter. However, precision design to tune their physicochemical properties remains a serious challenge because the properties are governed by subtle π-π interactions among funct...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03584-1

    authors： Lu F,Takaya T,Iwata K,Kawamura I,Saeki A,Ishii M,Nagura K,Nakanishi T

    更新日期：2017-06-13 00:00:00

  • Multivariate Granger causality unveils directed parietal to prefrontal cortex connectivity during task-free MRI.

    abstract：:While a large body of research has focused on the study of functional brain "connectivity", few investigators have focused on directionality of brain-brain interactions which, in spite of the mostly bidirectional anatomical substrates, cannot be assumed to be symmetrical. We employ a multivariate Granger Causality-bas...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-23996-x

    authors： Duggento A,Passamonti L,Valenza G,Barbieri R,Guerrisi M,Toschi N

    更新日期：2018-04-03 00:00:00

  • Curcumin as therapeutics for the treatment of head and neck squamous cell carcinoma by activating SIRT1.

    abstract：:SIRT1 is one of seven mammalian homologs of Sir2 that catalyzes NAD(+)-dependent protein deacetylation. The aim of the present study is to explore the effect of SIRT1 small molecule activator on the anticancer activity and the underlying mechanism. We examined the anticancer activity of a novel oral agent, curcumin, w...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13429

    authors： Hu A,Huang JJ,Li RL,Lu ZY,Duan JL,Xu WH,Chen XP,Fan JP

    更新日期：2015-08-24 00:00:00

  • Association of hyperuricemia and gamma glutamyl transferase as a marker of metabolic risk in alcohol use disorder.

    abstract：:Excessive alcohol consumption leads to overproduction of urates and renal function plays a critical role in serum uric acid levels. We aimed to assess associations of hyperuricemia in patients with alcohol use disorder (AUD) and comparable Glomerular Filtration Rate (GFR). A total of 686 patients undergoing treatment ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-77013-1

    authors： Hernández-Rubio A,Sanvisens A,Bolao F,Pérez-Mañá C,García-Marchena N,Fernández-Prendes C,Muñoz A,Muga R

    更新日期：2020-11-18 00:00:00

  • CO oxidation and organic dyes degradation over graphene-Cu and graphene-CuNi catalysts obtained by solution combustion synthesis.

    abstract：:Graphene and its analogs in combination with metal nanopowders are among the most promising catalysts for various industry valuable processes. The newly obtained solution combustion synthesized graphene-Cu and graphene-CuNi nanocomposites were examined in heterogeneous catalysis of thermal activated CO oxidation and p...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72872-0

    authors： Khort A,Romanovski V,Leybo D,Moskovskikh D

    更新日期：2020-09-30 00:00:00

  • Laser- synthesized TiN nanoparticles as promising plasmonic alternative for biomedical applications.

    abstract：:Exhibiting a red-shifted absorption/scattering feature compared to conventional plasmonic metals, titanium nitride nanoparticles (TiN NPs) look as very promising candidates for biomedical applications, but these applications are still underexplored despite the presence of extensive data for conventional plasmonic coun...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37519-1

    authors： Popov AA,Tselikov G,Dumas N,Berard C,Metwally K,Jones N,Al-Kattan A,Larrat B,Braguer D,Mensah S,Da Silva A,Estève MA,Kabashin AV

    更新日期：2019-02-04 00:00:00

  • Mapping the local viscosity of non-Newtonian fluids flowing through disordered porous structures.

    abstract：:Flow of non-Newtonian fluids through topologically complex structures is ubiquitous in most biological, industrial and environmental settings. The interplay between local hydrodynamics and the fluid's constitutive law determines the distribution of flow paths. Consequently the spatial heterogeneity of the viscous resi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-68545-7

    authors： Eberhard U,Seybold HJ,Secchi E,Jiménez-Martínez J,Rühs PA,Ofner A,Andrade JS Jr,Holzner M

    更新日期：2020-07-16 00:00:00

  • Multivariate simulation framework reveals performance of multi-trait GWAS methods.

    abstract：:Burgeoning availability of genome-wide association study (GWAS) results and national biobank data has led to growing interest in performing multi-trait genetic analyses. Numerous multi-trait GWAS methods that exploit either summary statistics or individual-level data have been developed, but their relative performance...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38837

    authors： Porter HF,O'Reilly PF

    更新日期：2017-03-13 00:00:00

  • Clinical Implementation of Integrated Genomic Profiling in Patients with Advanced Cancers.

    abstract：:DNA focused panel sequencing has been rapidly adopted to assess therapeutic targets in advanced/refractory cancer. Integrated Genomic Profiling (IGP) utilising DNA/RNA with tumour/normal comparisons in a Clinical Laboratory Improvement Amendments (CLIA) compliant setting enables a single assay to provide: therapeutic ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-016-0021-4

    authors： Borad MJ,Egan JB,Condjella RM,Liang WS,Fonseca R,Ritacca NR,McCullough AE,Barrett MT,Hunt KS,Champion MD,Patel MD,Young SW,Silva AC,Ho TH,Halfdanarson TR,McWilliams RR,Lazaridis KN,Ramanathan RK,Baker A,Aldrich J,

    更新日期：2016-12-23 00:00:00

  • Phycosynthesis and Enhanced Photocatalytic Activity of Zinc Oxide Nanoparticles Toward Organosulfur Pollutants.

    abstract：:A novel eco-friendly procedure was developed to produce safer, stable and highly pure zinc oxide nanoparticles (ZnO NPs) using microalgae Chlorella extract. The ZnO NPs were synthesized simply using zinc nitrate and microalgae Chlorella extract which conducted at ambient conditions. In this recipe, microalgae Chlorell...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43368-3

    authors： Khalafi T,Buazar F,Ghanemi K

    更新日期：2019-05-03 00:00:00

  • Improvements in structural and optical properties of wafer-scale hexagonal boron nitride film by post-growth annealing.

    abstract：:Remarkable improvements in both structural and optical properties of wafer-scale hexagonal boron nitride (h-BN) films grown by metal-organic chemical vapor deposition (MOCVD) enabled by high-temperature post-growth annealing is presented. The enhanced crystallinity and homogeneity of the MOCVD-grown h-BN films grown a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-47093-9

    authors： Lee SH,Jeong H,Okello OFN,Xiao S,Moon S,Kim DY,Kim GY,Lo JI,Peng YC,Cheng BM,Miyake H,Choi SY,Kim JK

    更新日期：2019-07-22 00:00:00

  • The First 250 ms of Auditory Processing: No Evidence of Early Processing Negativity in the Go/NoGo Task.

    abstract：:Past evidence of an early Processing Negativity in auditory Go/NoGo event-related potential (ERP) data suggests that young adults proactively process sensory information in two-choice tasks. This study aimed to clarify the occurrence of Go/NoGo Processing Negativity and investigate the ERP component series related to ...

    journal_title：Scientific reports

    pub_type： 临床试验,杂志文章

    doi：10.1038/s41598-020-61060-9

    authors： Fogarty JS,Barry RJ,Steiner GZ

    更新日期：2020-03-04 00:00:00

  • Breast cancer cells exhibits specific dielectric signature in vitro using the open-ended coaxial probe technique from 200 MHz to 13.6 GHz.

    abstract：:Here we investigated the feasibility of using microwave spectroscopy for characterization of normal and breast cancer cell lines cultured in vitro. Healthy non-tumorigenic, MCF-10A and breast cancer, MDA-MB-231, Hs578T, T47D and MCF-7 cell lines were electrically characterized using the open-ended coaxial probe techni...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41124-1

    authors： Hussein M,Awwad F,Jithin D,El Hasasna H,Athamneh K,Iratni R

    更新日期：2019-03-18 00:00:00

  • A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response.

    abstract：:Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species of kiwifruit are susceptible to Pseudomonas syringa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03704-x

    authors： Jayaraman J,Choi S,Prokchorchik M,Choi DS,Spiandore A,Rikkerink EH,Templeton MD,Segonzac C,Sohn KH

    更新日期：2017-06-15 00:00:00

  • Automated single cell isolation from suspension with computer vision.

    abstract：:Current robots can manipulate only surface-attached cells seriously limiting the fields of their application for single cell handling. We developed a computer vision-based robot applying a motorized microscope and micropipette to recognize and gently isolate intact individual cells for subsequent analysis, e.g., DNA/R...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20375

    authors： Ungai-Salánki R,Gerecsei T,Fürjes P,Orgovan N,Sándor N,Holczer E,Horvath R,Szabó B

    更新日期：2016-02-09 00:00:00

  • Microglia retard dengue virus-induced acute viral encephalitis.

    abstract：:Patients with dengue virus (DENV) infection may also present acute viral encephalitis through an unknown mechanism. Here, we report that encephalitic DENV-infected mice exhibited progressive hunchback posture, limbic seizures, limbic weakness, paralysis, and lethality 7 days post-infection. These symptoms were accompa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep27670

    authors： Tsai TT,Chen CL,Lin YS,Chang CP,Tsai CC,Cheng YL,Huang CC,Ho CJ,Lee YC,Lin LT,Jhan MK,Lin CF

    更新日期：2016-06-09 00:00:00

  • Single-cell, single-mRNA analysis of Ccnb1 promoter regulation.

    abstract：:Promoter activation drives gene transcriptional output. Here we report generating site-specifically integrated single-copy promoter transgenes and measuring their expression to indicate promoter activities at single-mRNA level. mRNA counts, Pol II density and Pol II firing rates of the Ccnb1 promoter transgene resembl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02240-y

    authors： Vishnoi N,Yao J

    更新日期：2017-05-18 00:00:00

  • Enhancing participation to health screening campaigns by group interactions.

    abstract：:Improving the prevention efficacy of health screening campaigns by increasing their attendance rate represents a challenge that calls for new strategies. This paper analyzes the response to a Pap test screening campaign of 155,000 women over the last decade. Using a mathematical model of statistical physics origins we...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep09904

    authors： Burioni R,Contucci P,Fedele M,Vernia C,Vezzani A

    更新日期：2015-04-23 00:00:00

  • Study of Structural stability and formation mechanisms in DSPC and DPSM liposomes: A coarse-grained molecular dynamics simulation.

    abstract：:Liposomes or biological vesicles can be created from cholesterol, phospholipid, and water. Their stability is affected by their phospholipid composition which can influence disease treatment and drug delivery efficacy. In this study, the effect of phospholipid type on the formation and stability of liposomes using coa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-58730-z

    authors： Hashemzadeh H,Javadi H,Darvishi MH

    更新日期：2020-02-04 00:00:00

  • A p-i-n junction diode based on locally doped carbon nanotube network.

    abstract：:A p-i-n junction diode constructed by the locally doped network of single-walled carbon nanotubes (SWNTs) was investigated. In this diode, the two opposite ends of the SWNT-network channel were selectively doped by triethyloxonium hexachloroantimonate (OA) and polyethylenimine (PEI) to obtain the air-stable p- and n-t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep23319

    authors： Liu X,Chen C,Wei L,Hu N,Song C,Liao C,He R,Dong X,Wang Y,Liu Q,Zhang Y

    更新日期：2016-03-21 00:00:00

  • Characterization of the Escherichia coli σ(S) core regulon by Chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis.

    abstract：:In bacteria, selective promoter recognition by RNA polymerase is achieved by its association with σ factors, accessory subunits able to direct RNA polymerase "core enzyme" (E) to different promoter sequences. Using Chromatin Immunoprecipitation-sequencing (ChIP-seq), we searched for promoters bound by the σ(S)-associa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep10469

    authors： Peano C,Wolf J,Demol J,Rossi E,Petiti L,De Bellis G,Geiselmann J,Egli T,Lacour S,Landini P

    更新日期：2015-05-28 00:00:00

  • Author Correction: Summertime Primary and Secondary Contributions to Southern Ocean Cloud Condensation Nuclei.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-019-46788-3

    authors： Fossum KN,Ovadnevaite J,Ceburnis D,Dall'Osto M,Marullo S,Bellacicco M,Simó R,Liu D,Flynn M,Zuend A,O'Dowd C

    更新日期：2019-07-18 00:00:00

  • Three tandem promoters, together with IHF, regulate growth phase dependent expression of the Escherichia coli kps capsule gene cluster.

    abstract：:In this study we characterise three tandem promoters (PR1-1, PR1-2 and PR1-3) within the PR1 regulatory region of the Escherichia coli kps capsule gene cluster. Transcription from promoter PR1-2 was dependent on the activity of the upstream promoter PR1-1, which activated PR1-2 via transcription coupled DNA supercoili...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17891-0

    authors： Jia J,King JE,Goldrick MC,Aldawood E,Roberts IS

    更新日期：2017-12-20 00:00:00

  • Automatic graphene transfer system for improved material quality and efficiency.

    abstract：:In most applications based on chemical vapor deposition (CVD) graphene, the transfer from the growth to the target substrate is a critical step for the final device performance. Manual procedures are time consuming and depend on handling skills, whereas existing automatic roll-to-roll methods work well for flexible su...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep21676

    authors： Boscá A,Pedrós J,Martínez J,Palacios T,Calle F

    更新日期：2016-02-10 00:00:00

  • Histone deacetylase-mediated regulation of the antimicrobial peptide hBD2 differs in intestinal cell lines and cultured tissue.

    abstract：:Histone deacetylase inhibition (HDACi) has been suggested as a promising approach to bolster TLR-mediated induction of antimicrobial peptides such as human β-defensin 2 (hBD2). In inflammatory bowel disease (IBD), Crohn's disease (CD) patients display an attenuated expression of hBD2 as compared to ulcerative colitis ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-31125-x

    authors： Stebe-Frick S,Ostaff MJ,Stange EF,Malek NP,Wehkamp J

    更新日期：2018-08-27 00:00:00