Abstract:
BACKGROUND:Post-translational modification (PTM) of proteins is central to many cellular processes across all domains of life, but despite decades of study and a wealth of genomic and proteomic data the biological function of many PTMs remains unknown. This is especially true for prokaryotic PTM systems, many of which have only recently been recognized and studied in depth. It is increasingly apparent that a deep sampling of abundance across a wide range of environmental stresses, growth conditions, and PTM types, rather than simply cataloging targets for a handful of modifications, is critical to understanding the complex pathways that govern PTM deposition and downstream effects. RESULTS:We utilized a deeply-sampled dataset of MS/MS proteomic analysis covering 9 timepoints spanning the Escherichia coli growth cycle and an unbiased PTM search strategy to construct a temporal map of abundance for all PTMs within a 400 Da window of mass shifts. Using this map, we are able to identify novel targets and temporal patterns for N-terminal N α acetylation, C-terminal glutamylation, and asparagine deamidation. Furthermore, we identify a possible relationship between N-terminal N α acetylation and regulation of protein degradation in stationary phase, pointing to a previously unrecognized biological function for this poorly-understood PTM. CONCLUSIONS:Unbiased detection of PTM in MS/MS proteomics data facilitates the discovery of novel modification types and previously unobserved dynamic changes in modification across growth timepoints.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Brown CW,Sridhara V,Boutz DR,Person MD,Marcotte EM,Barrick JE,Wilke COdoi
10.1186/s12864-017-3676-8subject
Has Abstractpub_date
2017-04-17 00:00:00pages
301issue
1issn
1471-2164pii
10.1186/s12864-017-3676-8journal_volume
18pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:The large amount of data produced by high-throughput sequencing poses new computational challenges. In the last decade, several tools have been developed for the identification of transcription and splicing factor binding sites. RESULTS:Here, we introduce the SeAMotE (Sequence Analysis of Motifs Enrichment)...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-925
更新日期:2014-10-23 00:00:00
abstract:BACKGROUND:Theileria parva is an intracellular parasite that causes a lymphoproliferative disease in cattle. It does so by inducing cancer-like phenotypes in the host cells it infects, although the molecular and regulatory mechanisms involved remain poorly understood. RNAseq data, and the resulting updated genome annot...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2444-5
更新日期:2016-02-20 00:00:00
abstract:BACKGROUND:Mango fruits contain a broad spectrum of phenolic compounds which impart potential health benefits; their biosynthesis is catalysed by enzymes in the phenylpropanoid-flavonoid (PF) pathway. The aim of this study was to reveal the variability in genes involved in the PF pathway in three different mango variet...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1784-x
更新日期:2015-07-30 00:00:00
abstract::The draft genome sequence of cattle (Bos taurus) has now been analyzed by the Bovine Genome Sequencing and Analysis Consortium and the Bovine HapMap Consortium, which together represent an extensive collaboration involving more than 300 scientists from 25 different countries. ...
journal_title:BMC genomics
pub_type: 社论
doi:10.1186/1471-2164-10-193
更新日期:2009-04-24 00:00:00
abstract:BACKGROUND:Analysis of single nucleotide polymorphisms (SNPs) derived from whole-genome studies allows for rapid evaluation of genome-wide diversity, and genomic epidemiology studies of Plasmodium falciparum provide insights into parasite population structure, gene flow, drug resistance and vaccine development. In area...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-719
更新日期:2014-08-26 00:00:00
abstract:BACKGROUND:The human zinc finger protein 191 (ZNF191) is a member of the SCAN domain family of Krüppel-like zinc finger transcription factors. ZNF191 shows 94% identity to its mouse homologue zinc finger protein 191(Zfp191), which is the most highly conserved among the human-mouse SCAN family member orthologues pairs. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-241
更新日期:2009-05-22 00:00:00
abstract:BACKGROUND:Reads assignment to taxonomic units is a key step in microbiome analysis pipelines. To date, accurate taxonomy annotation of 16S reads, particularly at species rank, is still challenging due to the short size of read sequences and differently curated classification databases. The close phylogenetic relations...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5914-8
更新日期:2019-07-08 00:00:00
abstract:BACKGROUND:The laying hen model of spontaneous epithelial ovarian cancer (EOC) is unique in that it is the only model that enables observations of early events in disease progression and is therefore also uniquely suited for chemoprevention trials. Previous studies on the effect of dietary flaxseed in laying hens have ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-709
更新日期:2014-08-24 00:00:00
abstract:BACKGROUND:Free asparagine is the precursor for acrylamide formation during cooking and processing of grains, tubers, beans and other crop products. In wheat grain, free asparagine, free glutamine and total free amino acids accumulate to high levels in response to sulphur deficiency. In this study, RNA-seq data were ac...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5991-8
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:To develop evolutionary models for the free living bacterium Alteromonas the genome sequences of isolates of the genus have been extensively analyzed. However, the main genetic exchange drivers in these microbes, conjugative elements (CEs), have not been considered in detail thus far. In this work, CEs have ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3461-0
更新日期:2017-01-05 00:00:00
abstract:BACKGROUND:Aphid (Macrosiphoniella sanbourni) stress drastically influences the yield and quality of chrysanthemum, and grafting has been widely used to improve tolerance to biotic and abiotic stresses. However, the effect of grafting on the resistance of chrysanthemum to aphids remains unclear. Therefore, we used the ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6158-3
更新日期:2019-10-25 00:00:00
abstract:BACKGROUND:Serine/threonine kinase 33 (STK33) has been shown to be conserved across all major vertebrate classes including reptiles, mammals, amphibians and fish, suggesting its importance within vertebrates. It has been shown to phosphorylate vimentin and might play a role in spermatogenesis and organ ontogenesis. In ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1769-9
更新日期:2015-07-22 00:00:00
abstract:BACKGROUND:The purpose of this research was to develop a novel information theoretic method and an efficient algorithm for analyzing the gene-gene (GGI) and gene-environmental interactions (GEI) associated with quantitative traits (QT). The method is built on two information-theoretic metrics, the k-way interaction inf...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-509
更新日期:2009-11-04 00:00:00
abstract:BACKGROUND:The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an attractive goal. However, large sample numbers are a pre-requisite for the identificati...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-16
更新日期:2012-01-11 00:00:00
abstract:BACKGROUND:Prostaglandin E2 (PGE2) is involved in several chronic inflammatory diseases including periodontitis, which causes loss of the gingival tissue and alveolar bone supporting the teeth. We have previously shown that tumor necrosis factor alpha (TNFalpha) induces PGE2 synthesis in gingival fibroblasts. In this s...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-241
更新日期:2010-04-15 00:00:00
abstract:BACKGROUND:Dimension reduction is a critical issue in the analysis of microarray data, because the high dimensionality of gene expression microarray data set hurts generalization performance of classifiers. It consists of two types of methods, i.e. feature selection and feature extraction. Principle component analysis ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-S2-S24
更新日期:2008-09-16 00:00:00
abstract:BACKGROUND:Uranium (U) is a naturally occurring radionuclide that has been found in the aquatic environment due to anthropogenic activities. Exposure to U may pose risk to aquatic organisms due to its radiological and chemical toxicity. The present study aimed to characterize the chemical toxicity of U in Atlantic salm...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-694
更新日期:2014-08-20 00:00:00
abstract:BACKGROUND:Limited nutrient availability during development is associated with metabolic diseases in adulthood. The molecular cause for these defects is unclear. Here, we investigate if transcriptional changes caused by developmental malnutrition reveal an early response that can be linked to metabolism and metabolic d...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1654-6
更新日期:2015-06-04 00:00:00
abstract:BACKGROUND:The increasing resistance of Plasmodium, the malaria parasites, to multiple commonly used drugs has underscored the urgent need to develop effective antimalarial drugs and vaccines. The new direction of genomics-driven target discovery has become possible with the completion of parasite genome sequencing, wh...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-S3-S13
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Understanding the evolution of the vertebrate pancreas is key to understanding its functions. The chondrichthyes (cartilaginous fish such as sharks and rays) have often been suggested to possess the most ancient example of a distinct pancreas with both hormonal (endocrine) and digestive (exocrine) roles. The...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-1074
更新日期:2014-12-06 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa is an important opportunistic pathogen responsible for many infections in hospitalized and immunocompromised patients. Previous reports estimated that approximately 10% of its 6.6 Mbp genome varies from strain to strain and is therefore referred to as "accessory genome". Elements withi...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-737
更新日期:2014-08-29 00:00:00
abstract:BACKGROUND:The σ(54) subunit controls a unique class of promoters in bacteria. Such promoters, without exception, require enhancer binding proteins (EBPs) for transcription initiation. Desulfovibrio vulgaris Hildenborough, a model bacterium for sulfate reduction studies, has a high number of EBPs, more than most sequen...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-2176-y
更新日期:2015-11-10 00:00:00
abstract:BACKGROUND:Genetic diversity within a species reflects population evolution, ecology, and ability to adapt. Genome-wide population surveys of both natural and introduced populations provide insights into genetic diversity, the evolutionary processes and the genetic basis underlying local adaptation. Grass carp is the m...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5872-1
更新日期:2019-06-07 00:00:00
abstract:BACKGROUND:The cotton (Gossypium spp.) fiber cell is an important unicellular model for studying cell differentiation. There is evidence suggesting that phosphorylation is a critical post-translational modification involved in regulation of a wide range of cell activities. Nevertheless, the sites of phosphorylation in ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-466
更新日期:2014-06-12 00:00:00
abstract::Following the publication of the original article [1], the authors reported an error in Fig. 2 of the PDF version of their article. ...
journal_title:BMC genomics
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12864-019-6419-1
更新日期:2019-12-31 00:00:00
abstract:BACKGROUND:Babesia bovis is an apicomplexan parasite that causes babesiosis in infected cattle. Genomes of pathogens contain promising information that can facilitate the development of methods for controlling infections. Although the genome of B. bovis is publically available, annotated gene models are not highly reli...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-678
更新日期:2014-08-13 00:00:00
abstract:BACKGROUND:About 6 million Americans suffer from heart failure and 70% of heart failure cases are caused by myocardial infarction (MI). Following myocardial infarction, increased cytokines induce two major types of macrophages: classically activated macrophages which contribute to extracellular matrix destruction and a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S6-S21
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Despite substantial progress in mosquito genomic and genetic research, few cis-regulatory elements (CREs), DNA sequences that control gene expression, have been identified in mosquitoes or other non-model insects. Formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, FAIRE-seq, i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2468-x
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:Low iron bioavailability is a common feature of ocean surface water and therefore micro-algae developed original strategies to optimize iron uptake and metabolism. The marine picoeukaryotic green alga Ostreococcus tauri is a very good model for studying physiological and genetic aspects of the adaptation of ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2666-6
更新日期:2016-05-03 00:00:00
abstract:BACKGROUND:Pekin duck products have become popular in Asia over recent decades and account for an increasing market share. However, the genetic mechanisms affecting carcass growth in Pekin ducks remain unknown. This study aimed to identify quantitative trait loci affecting body size and carcass yields in Pekin ducks. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5379-1
更新日期:2019-01-03 00:00:00