Abstract:
BACKGROUND:EBOV outbreaks continue to threaten the world due to the absence of effective vaccines and therapeutics. Easy-to-use and rapid diagnostic tests for EBOV are highly desired for prevention and control of the EVD epidemic. METHODS:Escherichia coli expression system was used to express VP40 protein of Zaire Ebola virus (ZEBOV) as water-soluble protein upon optimization of temperature, time, and IPTG concentration. VP40 protein was purified through Ni-NTA affinity chromatography and applied to immunize rabbits for immunogenicity analysis. Rabbit polyclonal antibodies against VP40 protein was produced and antibody response was analyzed using Western blot, enzyme-linked immunosorbent assay (ELISA), and immunoperoxidase monolayer assay (IPMA). RESULTS:Recombinant full-length VP40 protein of ZEBOV was expressed in E. coli Rosetta (DE3) cells as water-soluble protein. Analysis of antibody responses showed that rabbit polyclonal antibodies against VP40 protein could react specifically with this E. coli-expressed protein in Western blot and ELISA, and antibody titers in ELISA reached 1:25600. Besides, the produced rabbit polyclonal antibodies bound to VP40 proteins eukaryotically expressed by transfecting pcDNA-eGFP-VP40 into BHK-21 cells in IPMA. CONCLUSION:These results show that the prokaryotically expressed VP40 protein has high immunogenicity and can be used as diagnostic antigen in ELISA and other immunoassays. The strategy used in this study might be a potential way for preparing diagnostic agents for prevention and control of exotic diseases.
journal_name
Arch Iran Medjournal_title
Archives of Iranian medicineauthors
Wang Y,Liu X,Tao L,Xu P,Gao X,Li H,Yang Z,Wu Wdoi
0172004/AIM.0011subject
Has Abstractpub_date
2017-04-01 00:00:00pages
246-250issue
4eissn
1029-2977issn
1735-3947pii
0011journal_volume
20pub_type
杂志文章abstract:BACKGROUND:We aimed to examine cases of brucellosis that presented with severe thrombocytopenia and hemorrhagic diathesis. METHODS:A total of 10 brucellosis cases with severe thrombocytopenia were included in this case-series study. Patients' files were reviewed for their clinical and laboratory findings, as well as c...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:012155/AIM.0011
更新日期:2012-05-01 00:00:00
abstract::Following the Islamic Revolution of 1979 in Iran, the trend of migration of physicians from the country continued. The total number of Iranian physicians migrated to the United States (US) increased from 1625 before revolution in 1974, to 5045 in 2010, thirty years post-revolution. The percentage of medical graduates ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0131610/AIM.008
更新日期:2013-10-01 00:00:00
abstract::Following the establishment of Gastroenterology and Hepatology Fellowship Programs in 1987, significant developments in research and health care delivery have been achieved. The number of published articles has increased significantly and now more than 10 approved research centers are involved in several longitudinal ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章,评审
doi:
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:Glutaric Aciduria type 1 (GA1) is a metabolic inborn error and is characterized by increasing excursion of glutaric acid and its derivates, presented in microcephaly and dystonia. The disease is resulted from mutational inactivation in the GCDH gene encoding the glutaryl-CoA dehydrogenase. The defective enzy...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0141709/AIM.009
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:Coronavirus disease 2019 (COVID-19) has been widespread since late December 2019, with several symptoms related to the upper and lower respiratory system. However, its cardiac manifestations are less frequently studied. We aimed to analyze the available COVID-19 data on acute cardiac injury, using troponin a...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章,meta分析
doi:10.34172/aim.2020.107
更新日期:2020-11-01 00:00:00
abstract::Hepatitis B virus is a hepatotropic virus that causes acute and chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma; it is responsible for more than one million deaths annually worldwide despite hepatitis B vaccination. Until now, there are eight known genotypes (A-H).Clinical course of chronic hepatitis ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:08114/AIM.0019
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:The childhood obesity epidemic is one of the most serious global health challenges, and many relevant policies have been designed and implemented. Regarding health equity policy-making, it is important to adopt proper interventional strategies, including childhood obesity policies. The purpose of this study ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Human rotavirus genotypes G1-G4, G9, P[4] and P[8] are major worldwide causes of acute gastroenteritis in children. Rotavirus genotype G1P[8] is predominant in many countries. In this study, the genotypic diversity of group A rotaviruses were detected in children <5 years of age who were treated for dehydrat...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:011141/AIM.009
更新日期:2011-01-01 00:00:00
abstract::The coronavirus infection is an evolving pandemic with high morbidity and mortality, especially in people with comorbidities. The case fatality rate (CFR) is 9.2% in the presence of diabetes, while it is 1.4% in those without any comorbidity. Diabetes is a prevalent disease globally; hence, healthcare professionals ar...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:10.34172/aim.2020.61
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Alzheimer and Parkinson diseases (AD and PD) are the two most important neurodegenerative disorders. This paper aims to determine the possible molecular linkage between these two common neurodegenerative diseases by a combination of computational investigations. METHODS:According to our aim, common sets of ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0161902/AIM.007
更新日期:2016-02-01 00:00:00
abstract::Markers of bone turnover are becoming an important tool for practitioners in the management of osteoporosis. Therefore, it is essential to establish a reference database of the markers before using them in various clinical settings. A total of 785 individuals (37% males, 63% females) without apparent or suggested abno...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章,多中心研究
doi:08112/AIM.0015
更新日期:2008-03-01 00:00:00
abstract::Vitamin D is a pro-hormone that plays an important role in body calcium regulation. Vitamin D toxicity occurs rarely due to its wide therapeutic index. A 38-year-old Iranian man was admitted to our hospital with a diagnosis of vitamin D toxicity. Laboratory test revealed hypercalcemia and elevated 25-hydroxyvitamin D ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0161908/AIM.0013
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Postmenopausal bleeding is a clinically important complaint in general gynecologic practice. The incidence of spontaneous postmenopausal bleeding in the general population is approximately 10 % immediately after menopause, and 5 % in all menopausal women. OBJECTIVES:The study was aimed to reveal the histopa...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0131611/AIM.0011
更新日期:2013-11-01 00:00:00
abstract::Many reported scientific misconducts included duplication, data fabrication and falsification; but recent fake peer reviews have become strange news for many researchers worldwide. Using gmail and other non-academic emails to correspond with journals should be highly prohibited. Indeed, the dark part of this story is ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:The standard treatment for decompensated liver cirrhosis is liver transplantation. However, it has several limitations. Recent animal studies suggest that bone marrow stem cell transplantation can lead to regression of liver fibrosis. The objective of this study was to determine the safety and feasibility of...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:07104/AIM.008
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND:Polycystic ovary syndrome is not uncommon in women of reproductive age. We conducted this study to determine the clinical, hormonal, and biochemical effects of metformin therapy on girls with polycystic ovary syndrome. METHODS:In a self-controlled clinical trial conducted at the Gynecology Center of Yahyane...
journal_title:Archives of Iranian medicine
pub_type: 临床试验,杂志文章
doi:07102/AIM.0010
更新日期:2007-04-01 00:00:00
abstract::Acute hemorrhagic edema of infancy is a distinctive, cutaneous small vessel leukocytoclastic vasculitis of young children with dramatic characteristic skin findings. It is characterized by low- grade fever, erythematous edema, and purpuric lesions mainly on the face and extremities. Visceral involvement is uncommon, a...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:08111/AIM.0019
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Non-invasive ventilation (NIV) has brought about a significant change in care and treatment of respiratory distress syndrome (RDS) in very low birth weight (VLBW) neonates. The present study was designed and conducted to evaluate different strategies of initial respiratory support (IRS) in VLBW neonates hosp...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0172003/AIM.008
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:The present study reported a six-year follow up of patients with chronic myeloid leukemia who were on imatinib therapy. METHODS:We performed a retrospective study on a total of 417 patients diagnosed with chronic-phase, Philadelphia-positive (Ph+) chronic myeloid leukemia within six months before study entr...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:011146/AIM.004
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to investigate the association between Trp719Arg (rs20455) and Coronary Heart Disease (CHD), and also Coronary Heart Disease reduction in individuals with this SNP during statin therapy in southern Iran. It has been shown that rs20455, which could affect the function of kinesin ...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0151810/AIM.008
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Wrong beliefs about risky actions such as sunbathing and tanning are common due to media advertisements or general lack of knowledge. This work has focused on the knowledge and attitude of a group of citizens in Tehran, Iran and the protective actions they take regarding undesirable effects of the sun. M...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:011142/AIM.0011
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Unprotected sexual activity is an important reason for the currently growing incidence of HIV infection in Iran. Recognizing barriers to safe sexual practice and affordance of behavioral changes can improve planning for condom promotion. The main objective of our study is to evaluate the opinions of HIV-posi...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0121512/AIM.0010
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Osteoporosis has been frequently reported in patients with inflammatory bowel diseases, especially Crohn's disease. METHODS:All consecutive Crohn's disease patients who attended the GI Clinics at Shariati Hospital, Tehran, Iran, from 2004 to 2007 were evaluated. A BMD-DEXA assessment was performed for all p...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Oxidative stress is involved in the pathophysiology of diabetes mellitus. METHODS:In the present study, 68 patients with type 2 diabetes mellitus and 31 clinically healthy individuals were evaluated. The patients were divided into two groups. Group 1 included 29 patients without diabetic complications and g...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:Abnormal expression of microRNAs (miRNAs) plays an important role in development of several cancer types, including bladder cancer (BCa). However, the relationship between the ratio of miR-181/miR-100 and the prognosis of BCa has not been studied yet. The aim of this study was to evaluate the expression of m...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0161909/AIM.008
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:The Consolidated Standards for Reporting of Trials (CONSORT) statement is a device to standardize reporting and improve the quality of controlled trials. The aim of this study is to determine the quality of controlled clinical trial reports by national peer-reviewed journals in Iran. METHODS:In a cross-sect...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:013161/AIM.007
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Studies on the association between legume intake and metabolic syndrome (MetS) are sparse. The objective of this study is to evaluate the association between legume intake, MetS, and its components. METHODS:This study was conducted on 80 subjects (48% female) with MetS as cases and 160 age and gender-matche...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:012159/AIM.005
更新日期:2012-09-01 00:00:00
abstract:UNLABELLED: BACKGROUND:Potato, a high- glycemic index (GI) food, is one of the most widely used starchy foods worldwide. Previous studies on the association of dietary intakes with stroke have mostly focused on the dietary GI and there is no information regarding the association between potato consumption and risk of...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:013163/AIM.009
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:HIV infection affects all body organs including kidney. Since the frequency of HIV-related renal disorders is unknown in Iran and the number of HIV-infected patients is increasing, this study was conducted for the first time in Iran to assess the frequency of electrolyte imbalance, renal failure, and protein...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:07103/AIM.0010
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Neuropsychological manifestations are present in 60% of patients with Systemic Lupus Erythematosus (SLE) among which cognitive dysfunction is the most common. This study aims to determine the prevalence of cognitive disorders in SLE patients, and the relationship between cognitive disorder domains and depres...
journal_title:Archives of Iranian medicine
pub_type: 杂志文章
doi:0161904/AIM.006
更新日期:2016-04-01 00:00:00