Abstract:
AIM:To evaluate molecular mechanisms of tissue-protective effects of antioxidants selenomethionine (SeMet) and D-pantethine (D-Pt) applied in combination with doxorubicin (Dx) in B16 melanoma-bearing-mice. METHODS:Impact of the chemotherapy scheme on a survival of tumor-bearing animals, general nephro- and hepatotoxicity, blood cell profile in vivo, and ROS content in B16 melanoma cells in vitro was compared with the action of Dx applied alone. Nephrotoxicity of the drugs was evaluated by measuring creatinine indicator assay, hepatotoxicity was studied by measuring the activity of ALT/AST enzymes, and myelotoxicity was assessed by light microscopic analysis of blood smears. Changes in ROS content in B16 melanoma cells under Dx, SeMet, and D-Pt action in vitro were measured by incubation with fluorescent dyes dihydrodichlorofluoresceindiacetate (DCFDA, H2O2-specific) and dihydroethidium (DHE, O2--specific), and further analysis at FL1 (DCFDA) or FL2 channels (DHE) of FACScan flow cytometer. The impact of aforementioned compounds on functional status of mitochondria was measured by Rhodamine 123 assay and further analysis at FL1 channel of FACScan flow cytometer. RESULTS:Selenomethionine (1200 µg/kg) and D-pantethine (500 mg/kg) in combination with Dx (10 mg/kg) significantly reduced tumor-induced neutrophilia, lymphocytopenia, and leukocytosis in comparison to Dx treatment alone. Moreover, SeMet and D-Pt decreased several side effects of Dx, namely an elevated creatinine level in blood and monocytosis, thus normalizing health conditions of B16 melanoma-bearing animals. CONCLUSIONS:Our results showed that antioxidants selenomethionine and D-pantethine possess significant nephroprotective and myeloprotective activity toward Dx action on murine B16 melanoma in vivo, but fail to boost a survival of B16 melanoma-bearing animals. The observed cytoprotective effects of studied antioxidants are not directly connected with their ROS scavenging.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Panchuk RR,Skorokhyd NR,Kozak YS,Lehka LV,Moiseenok AG,Stoika RSdoi
10.3325/cmj.2017.58.171subject
Has Abstractpub_date
2017-04-14 00:00:00pages
171-184issue
2eissn
0353-9504issn
1332-8166journal_volume
58pub_type
杂志文章abstract:AIM:To detect polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci, including 6 new loci, in a reference database of male population of Bosnia and Herzegovina, as well as to assess the importance of increasing the number of Y-STR loci utilized in forensic DNA analysis. METHODS:The reference sample consiste...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2013.54.286
更新日期:2013-06-01 00:00:00
abstract:AIM:To evaluate the results of current reforms in Macedonian health sector. METHOD:Description and situation analysis, covering the period 1991-1997, are focused on demographic and vital indicators, morbidity and mortality data, elements of health care system, legislation, health insurance, health care financing, and ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:AIM:To investigate whether socioeconomic inequalities at a micro-scale, through their effect on major health risk factors and other health indicators, contribute to health status in an isolated island population with demonstrated reduced genetic and environmental variability. METHODS:This cross-sectional study was per...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract:AIM:To determine factors influencing voluntary counseling and premarital testing on human immunodeficiency virus (HIV) in Malawi. METHOD:We analyzed the data collected by the Malawi Demographic and Health Survey (MDHS) 2000 to determine the likelihood of Malawi population to accept HIV testing. The MDHS was a nationwi...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract:AIM:To determine the clinical profile, burden of risk factors, and quality of care among patients hospitalized for an acute myocardial infarction (AMI) with special focus on gender differences. METHODS:The study included 256 AMI patients admitted to the Coronary Care Unit of "Mother Teresa" hospital in Tirana during 2...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2015.56.542
更新日期:2015-12-01 00:00:00
abstract::We present a paternity testing case in which a double incompatibility was found for two short tandem repeat (STR) markers, human fibrinogen alpha (FGA) and Penta E. Analysis of the trio (mother, father, and daughter) included the amplification with a battery of 15 autosomal short tandem repeats (STR) by using a commer...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract:AIM:To determine overall cardiac effects of flavonoid catechin on ischemia-reperfusion injury in isolated rat hearts in vitro and in vivo. METHODS:After perfusing hearts at the pressure of 70 mm Hg, coronary perfusion was interrupted for 30 minutes and then re-established. There were four experimental groups, with 10 ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract:AIM:To test antinociceptive properties of botulinum toxin type A (BTX-A) in rats with carrageenan- and capsaicin-induced pain and inflammation. METHODS:Pain was provoked with carrageenan (1%) or capsaicin (0.1%) injection into the plantar surface of the rat paw-pad. The effect of BTX-A 5 U/kg on carrageenan- and capsa...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract:AIM:To determine types and frequency of CAPN3 mutations in 29 unrelated Croatian families, analyzed during 6-year prospective and ongoing genetic and epidemiological study of muscular dystrophies in Croatia. METHODS:Mutation analysis included allele-specific polymerase chain reaction (PCR) or combination of PCR and re...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::Academic medicine is usually considered to be a tripartite composition of teaching, research, and health care services delivery or patient care. The meaning of each component varies with interpretation. Developing countries such as Malawi face particular challenges in the field of academic medicine, including research...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract:AIM:To investigate missed opportunities to reveal existing but not formerly diagnosed coronary heart disease cases and related risk factors in primary health care. METHODS:The study comprised 850 people aged over 30 years with no known history of coronary heart disease, receiving health services from a primary care ce...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::We analyzed forensic DNA samples from four cases of sexual assault, using the Y-chromosome-specific human DNA markers and a panel of autosomal short tandem repeats (STRs). The presence of male contribution was evaluated by the analysis of the Amelogenin locus. A panel of tetrameric Y-STR (DYS19, DYS390, DYS391, DYS392...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract:AIM:To examine why the demand for substitutive voluntary health insurance in Germany is low. METHOD:A comparison of the benefits and costs of statutory and voluntary health insurance in Germany, based on a review of literature published in academic journals and books as well as gray literature. RESULTS:Employees in G...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract:AIM:To present the results obtained in the identification of human remains from World War II found in two mass graves in Ljubuški, Bosnia and Herzegovina. METHODS:Samples from 10 skeletal remains were collected. Teeth and femoral fragments were collected from 9 skeletons and only a femoral fragment from 1 skeleton. DN...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2015.56.257
更新日期:2015-06-01 00:00:00
abstract::A continuous rise in life expectancy has led to an increase in the number of senior citizens, now amounting to a fifth of the global population, and to a dramatic increase in the prevalence of diseases of the elderly. This review discusses the threat of dementia, a disease that imposes enormous financial burden on hea...
journal_title:Croatian medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2020-04-30 00:00:00
abstract:AIM:Oncostatin-M, a member of the gp 130 family of cytokines, has been associated with inflammation, connective tissue production, and extracellular matrix turnover. Since the reperfused heart is associated with an intense inflammatory reaction followed by scar formation, we tested the hypothesis that oncostatin-M is u...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract:AIM:To compare morbidity and mortality of patients with severe intra-abdominal infections after two types of surgical treatment, on-demand ("wait and see") relaparotomy and modified planned relaparotomy. METHODS:We prospectively analyzed the outcomes of 65 patients with severe peritonitis surgically treated in two Cro...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Hereditary factor XI (FXI) deficiency is a mild bleeding disorder, rare in the general population but relatively common among Ashkenazi Jews. The human F11 gene comprises 15 exons, spanning over 23 kb of the long arm of chromosome 4 (4q35). Homozygotes or compound heterozygotes typically show severe FXI deficiency, wh...
journal_title:Croatian medical journal
pub_type:
doi:
更新日期:2020-02-29 00:00:00
abstract::Health and health care provision are among the most important and politically sensitive public service areas. Politicians carefully incorporate health care program changes in their political agendas to gain votes. However, knowing health care priorities of the electoral body is not useful only to politicians, but also...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract:AIM:To develop an automated, high throughput extraction protocol in order to produce database eligible profiles from fingerprints and other low copy number (LCN) DNA sources. METHODS:Extraction of either purified control DNA or buccal cells, for example, with commercial kits was compared to extraction with a simple di...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract:AIM:To assess genetic diversity and genetic distances among isolated populations from Dagestan. METHODS:A cross-population genetic epidemiology design was applied in ethnically and demographically diverse isolates from Dagestan, some with more than 200 and some with less than 100 generations of demographical history s...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
abstract::The Revised European-American Classification of Lymphoid Neoplasms (REAL) classification has been validated by a multi-institutional study, and project data showed that it is both reproducible and clinically relevant. The new World Health Organization (WHO) Classification of Neoplastic Diseases of Hematopoietic and Ly...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract:AIM:To evaluate the accuracy of eye color prediction based on six IrisPlex single nucleotide polymorphisms (SNP) in a Slovenian population sample. METHODS:Six IrisPlex predictor SNPs (HERC2 - rs12913832, OCA2 - rs1800407, SLC45A2 - rs16891982 and TYR - rs1393350, SLC24A4 - rs12896399, and IRF4 - rs12203592) of 105 ind...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2013.54.381
更新日期:2013-08-01 00:00:00
abstract:AIM:Several amplification and detection formats for the analysis of short tandem repeat loci are readily available to the forensic laboratory. Careful consideration must be given to the throughput, sensitivity, concordance, data interpretation, facility requirements, and costs of operation. The Pennsylvania State Polic...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract:AIM:To assess the intrahepatic and extrahepatic bile duct diameter dilatation after laparoscopic cholecystectomy with magnetic resonance cholangiopancreatography. METHODS:Forty-eight patients (35 women, mean age 54.58±11.83 years) underwent laparoscopic cholecystectomy because of gallstones. The intrahepatic and extra...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2020-07-05 00:00:00
abstract:AIM:To evaluate long-term cognitive consequences of subarachnoid hemorrhage with good outcome and the opinion of patients and their relatives about these consequences. METHODS:The study included 10 patients surgically treated for subarachnoid hemorrhage due to the rupture of aneurysm of the anterior communicating arte...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract:AIM:To assess the quality of diabetes care provided by family medicine teams in primary health centers in Bosnia and Herzegovina (BH) through a medical audit, addressing the extent to which clinical practice complied with pre-determined explicit criteria of long-term management. METHOD:Retrospective analysis included ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2008.49.757
更新日期:2008-12-01 00:00:00
abstract:AIM:To assess the relationship between cognitive functions, severity of depressive symptoms, and expression of interleukin 1 (IL)-1 in patients treated with systemic anticancer therapy. METHODS:This prospective study, conducted in 2017-2018, involved 55 patients (56% men) subjected to systemic anticancer therapy. Fort...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:
更新日期:2019-04-30 00:00:00
abstract::We report on the 10-year follow-up and clinical, cytogenetic, and molecular investigation of a girl admitted for evaluation because of speech delay, learning difficulties, aggressive behavior, and dysmorphic facial features that included high forehead, round face, epicanthic folds, low-set dysplastic ears, flat nasal ...
journal_title:Croatian medical journal
pub_type: 杂志文章
doi:10.3325/cmj.2011.52.415
更新日期:2011-06-01 00:00:00
abstract::The generally accepted structure of a scientific paper is four sections, an introduction, a methods section, the results, and a discussion. This so-called IMRaD format is, with a few small variations, found in most research articles in biomedical journals. However, as a guide for someone writing up research data for t...
journal_title:Croatian medical journal
pub_type: 社论
doi:
更新日期:2002-06-01 00:00:00