Abstract:
:The kinetoplast (k), the uniquely packaged mitochondrial DNA of trypanosomatid protists is formed by a catenated network of minicircles and maxicircles that divide and segregate once each cell cycle. Although many proteins involved in kDNA replication and segregation are now known, several key steps in the replication mechanism remain uncharacterized at the molecular level, one of which is the nabelschnur or umbilicus, a prominent structure which in the mammalian parasite Trypanosoma brucei connects the daughter kDNA networks prior to their segregation. Here we characterize an M17 family leucyl aminopeptidase metalloprotease, termed TbLAP1, which specifically localizes to the kDNA disk and the nabelschur and represents the first described protein found in this structure. We show that TbLAP1 is required for correct segregation of kDNA, with knockdown resulting in delayed cytokinesis and ectopic expression leading to kDNA loss and decreased cell proliferation. We propose that TbLAP1 is required for efficient kDNA division and specifically participates in the separation of daughter kDNA networks.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
Peña-Diaz P,Vancová M,Resl C,Field MC,Lukeš Jdoi
10.1371/journal.ppat.1006310subject
Has Abstractpub_date
2017-04-07 00:00:00pages
e1006310issue
4eissn
1553-7366issn
1553-7374pii
PPATHOGENS-D-16-01055journal_volume
13pub_type
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