Evolution of the endomembrane systems of trypanosomatids - conservation and specialisation.

Abstract:

:Parasite surfaces support multiple functions required for survival within their hosts, and maintenance and functionality of the surface depends on membrane trafficking. To understand the evolutionary history of trypanosomatid trafficking, where multiple lifestyles and mechanisms of host interactions are known, we examined protein families central to defining intracellular compartments and mediating transport, namely Rabs, SNAREs and RabGAPs, across all available Euglenozoa genomes. Bodonids possess a large trafficking repertoire, which is mainly retained by the Trypanosoma cruzi group, with extensive losses in other lineages, particularly African trypanosomes and phytomonads. There are no large-scale expansions or contractions from an inferred ancestor, excluding direct associations between parasitism or host range. However, we observe stepwise secondary losses within Rab and SNARE cohorts (but not RabGAPs). Major changes are associated with endosomal and late exocytic pathways, consistent with the diversity in surface proteomes between trypanosomatids and mechanisms of interaction with the host. Along with the conserved core family proteins, several lineage-specific members of the Rab (but not SNARE) family were found. Significantly, testing predictions of SNARE complex composition by proteomics confirms generalised retention of function across eukaryotes.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Venkatesh D,Boehm C,Barlow LD,Nankissoor NN,O'Reilly A,Kelly S,Dacks JB,Field MC

doi

10.1242/jcs.197640

subject

Has Abstract

pub_date

2017-04-15 00:00:00

pages

1421-1434

issue

8

eissn

0021-9533

issn

1477-9137

pii

jcs.197640

journal_volume

130

pub_type

杂志文章
  • Regulatory subunits of cyclic AMP-dependent protein kinase: presence in granules and secretion by exocrine and endocrine cells.

    abstract::Cyclic AMP-dependent protein kinase (cAPK) is the intracellular mediator of signal transduction events involving the adenylate cyclase-cyclic AMP system. A monoclonal antibody (MAb BB1) to the type II regulatory subunit (RII) of cAPK was used in a post-embedding immunogold-labeling procedure to determine the ultrastru...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Hand AR,Mednieks MI

    更新日期:1989-08-01 00:00:00

  • X-ray microanalysis of HeLa S3 Cells. I. Instrumental calibration and analysis of randomly growing cultures.

    abstract::Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Stan...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Warley A,Stephen J,Hockaday A,Appleton TC

    更新日期:1983-03-01 00:00:00

  • DNA replication-dependent intranuclear relocation of double minute chromatin.

    abstract::Double minutes (DMs) seen in a substantial fraction of human tumors are the cytogenetic manifestation of gene amplification which renders the tumor cells advantageous for growth and survival. DMs are acentric and atelomeric chromatin composed of circular DNA. In this study, we found they showed a remarkable relocation...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Itoh N,Shimizu N

    更新日期:1998-11-01 00:00:00

  • Cofilin takes the lead.

    abstract::Cofilin has emerged as a key regulator of actin dynamics at the leading edge of motile cells. Through its actin-severing activity, it creates new actin barbed ends for polymerization and also depolymerizes old actin filaments. Its function is tightly regulated in the cell. Spatially, its activity is restricted by othe...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01631

    authors: DesMarais V,Ghosh M,Eddy R,Condeelis J

    更新日期:2005-01-01 00:00:00

  • Cholesterol depletion facilitates ubiquitylation of NPC1 and its association with SKD1/Vps4.

    abstract::Niemann-Pick disease type C (NPC) is an inherited lipid storage disorder caused by mutations in NPC1 or NPC2. NPC1 is a polytopic glycoprotein that contains a sterol-sensing domain, whereas NPC2 is a soluble protein that contains an MD-2-like lipid-recognition domain. In the current study, we addressed the hypothesis ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02993

    authors: Ohsaki Y,Sugimoto Y,Suzuki M,Hosokawa H,Yoshimori T,Davies JP,Ioannou YA,Vanier MT,Ohno K,Ninomiya H

    更新日期:2006-07-01 00:00:00

  • MAP-1B/TAU functional redundancy during laminin-enhanced axonal growth.

    abstract::When cultured cerebellar macroneurons develop attached to a laminin-containing substrate or after the acute addition of laminin to the tissue culture medium, there is an acceleration in the rate and extent of axonal elongation. Furthermore, laminin is capable of inducing axonal formation and microtubule stabilization ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: DiTella MC,Feiguin F,Carri N,Kosik KS,Cáceres A

    更新日期:1996-02-01 00:00:00

  • Assembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody.

    abstract::Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii, and found that it reacts with a single flagellar glycoprotein of about 230 kDa. Interestingly, immunofluorescence microscopy demonstra...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Nakamura S,Tanaka G,Maeda T,Kamiya R,Matsunaga T,Nikaido O

    更新日期:1996-01-01 00:00:00

  • Suppression of tubulin tyrosine ligase during tumor growth.

    abstract::The C terminus of the tubulin alpha-subunit of most eukaryotic cells undergoes a cycle of tyrosination and detyrosination using two specific enzymes, a tubulin tyrosine ligase (TTL) and a tubulin carboxypeptidase. Although this enzyme cycle is conserved in evolution and exhibits rapid turnover, the meaning of this mod...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Lafanechère L,Courtay-Cahen C,Kawakami T,Jacrot M,Rüdiger M,Wehland J,Job D,Margolis RL

    更新日期:1998-01-01 00:00:00

  • Centriolar cycle of fused cells.

    abstract::Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structu...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Manandhar G,Onishchenko GE

    更新日期:1995-02-01 00:00:00

  • PINCH1 regulates cell-matrix and cell-cell adhesions, cell polarity and cell survival during the peri-implantation stage.

    abstract::PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking beta1 integrin or Ilk, loss of P...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02422

    authors: Li S,Bordoy R,Stanchi F,Moser M,Braun A,Kudlacek O,Wewer UM,Yurchenco PD,Fässler R

    更新日期:2005-07-01 00:00:00

  • Circadian rhythmicity in the activities of adenylate cyclase and phosphodiesterase in synchronously dividing and stationary-phase cultures of the achlorophyllous ZC mutant of Euglena gracilis.

    abstract::Key factors in the adenosine 3',5'-cyclic monophosphate (cyclic AMP) metabolic pathway are two enzymes responsible for its generation and degradation, namely, adenylate cyclase (AC) and phosphodiesterase (PDE). In LD: 12,12 (12 h light, 12 h dark), these enzymes were found to undergo bimodal, circadian variation of ac...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Tong J,Carre IA,Edmunds LN Jr

    更新日期:1991-10-01 00:00:00

  • The growth of Drosophila bristles and laterals is not restricted to the tip or base.

    abstract::The highly elongated bristles of Drosophila have proven to be a valuable model system for studying cellular morphogenesis. Extending bristles contain a series of large bundles of actin filaments juxtaposed to the plasma membrane and centrally located microtubules. Models to explain the extension of the bristle have pr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00065

    authors: Fei X,He B,Adler PN

    更新日期:2002-10-01 00:00:00

  • Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity.

    abstract::Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.192781

    authors: Atherton P,Lausecker F,Harrison A,Ballestrem C

    更新日期:2017-07-15 00:00:00

  • Identification of PTPsigma as an autophagic phosphatase.

    abstract::Macroautophagy is a dynamic process whereby portions of the cytosol are encapsulated in double-membrane vesicles and delivered to the lysosome for degradation. Phosphatidylinositol-3-phosphate (PtdIns3P) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The produc...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.080341

    authors: Martin KR,Xu Y,Looyenga BD,Davis RJ,Wu CL,Tremblay ML,Xu HE,MacKeigan JP

    更新日期:2011-03-01 00:00:00

  • Dynamic and reversible restructuring of the ER induced by PDMP in cultured cells.

    abstract::In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03058

    authors: Sprocati T,Ronchi P,Raimondi A,Francolini M,Borgese N

    更新日期:2006-08-01 00:00:00

  • Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells.

    abstract::We have demonstrated the possibility of cross-talk between E-cadherin and alphav integrins in breast carcinoma cells. Using the function-blocking anti-alphav monoclonal antibody 17E6, applied to monolayer cultures of breast cancer lines, it was found that treatment of cells possessing detergent-insoluble (implying att...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: von Schlippe M,Marshall JF,Perry P,Stone M,Zhu AJ,Hart IR

    更新日期:2000-02-01 00:00:00

  • Innervation regulates synaptic ribbons in lateral line mechanosensory hair cells.

    abstract::Failure to form proper synapses in mechanosensory hair cells, the sensory cells responsible for hearing and balance, leads to deafness and balance disorders. Ribbons are electron-dense structures that tether synaptic vesicles to the presynaptic zone of mechanosensory hair cells where they are juxtaposed with the post-...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.182592

    authors: Suli A,Pujol R,Cunningham DE,Hailey DW,Prendergast A,Rubel EW,Raible DW

    更新日期:2016-06-01 00:00:00

  • UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.

    abstract::In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.248815

    authors: Habicht J,Mooneyham A,Hoshino A,Shetty M,Zhang X,Emmings E,Yang Q,Coombes C,Gardner MK,Bazzaro M

    更新日期:2021-01-08 00:00:00

  • p31comet-mediated extraction of Mad2 from the MCC promotes efficient mitotic exit.

    abstract::Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31(comet) is a checkpoint antagonist in that its inhibition delays mitotic exit, whereas its overexpression overrides the checkpoint. How exactly p31(com...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.093286

    authors: Westhorpe FG,Tighe A,Lara-Gonzalez P,Taylor SS

    更新日期:2011-11-15 00:00:00

  • Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes.

    abstract::During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remain...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.009977

    authors: Khetani RS,Bickel SE

    更新日期:2007-09-01 00:00:00

  • The association of Plk1 with the astrin-kinastrin complex promotes formation and maintenance of a metaphase plate.

    abstract::Errors in mitotic chromosome segregation can lead to DNA damage and aneuploidy, both hallmarks of cancer. To achieve synchronous error-free segregation, mitotic chromosomes must align at the metaphase plate with stable amphitelic attachments to microtubules emanating from opposing spindle poles. The astrin-kinastrin (...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.251025

    authors: Geraghty Z,Barnard C,Uluocak P,Gruneberg U

    更新日期:2021-01-08 00:00:00

  • Rho-family GTPases: it's not only Rac and Rho (and I like it).

    abstract::The Rho-family proteins make up a major branch of the Ras superfamily of small GTPases. To date, 22 human genes encoding at least 25 proteins have been described. The best known 'classical' members are RhoA, Rac1 and Cdc42. Highly related isoforms of these three proteins have not been studied as intensively, in part b...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.01118

    authors: Wennerberg K,Der CJ

    更新日期:2004-03-15 00:00:00

  • The mechanism of Fc-mediated interaction of eosinophils with immobilized immune complexes. II. Identification of two membrane proteins, modified by the interaction.

    abstract::Human peripheral blood eosinophils attach to and flatten down onto antibody-coated surfaces and subsequently degranulate. An antibody-coated surface was prepared by treating a layer of agar, containing tetanus toxoid antigen and eosinophil chemotactic factor (ECF), with human anti-tetanus immunoglobin. Changes in eosi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Thorne KJ,Free J,Franks D,Oliver RC

    更新日期:1982-08-01 00:00:00

  • The subapical compartment: a traffic center in membrane polarity development.

    abstract::Spatially separated apical and basolateral plasma membrane domains that have distinct functions and molecular compositions are a characteristic feature of epithelial cell polarity. The subapical compartment (SAC), also known as the common endosome (CE), where endocytic pathways from both surfaces merge, plays a crucia...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.01217

    authors: Hoekstra D,Tyteca D,van IJzendoorn SC

    更新日期:2004-05-01 00:00:00

  • Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration.

    abstract::Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) act as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of th...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.204065

    authors: Lozano-Elena F,Planas-Riverola A,Vilarrasa-Blasi J,Schwab R,Caño-Delgado AI

    更新日期:2018-01-29 00:00:00

  • Chondrons from articular cartilage: I. Immunolocalization of type VI collagen in the pericellular capsule of isolated canine tibial chondrons.

    abstract::A heterogenous population of intact chondrons extracted from low-speed homogenates of canine tibial cartilage were stained by indirect immunofluorescence methods with a polyclonal antibody to type VI collagen. In each of the four chondron groups examined, anti-(type VI collagen) anti-serum was concentrated in the caps...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Poole CA,Ayad S,Schofield JR

    更新日期:1988-08-01 00:00:00

  • New aspects of nuclear calcium signalling.

    abstract::Nuclear calcium signalling has been a controversial battlefield for many years and the question of how permeable the nuclear pore complexes (NPCs) are to Ca2+ has been the subject of a particularly hot dispute. Recent data from isolated nuclei suggest that the NPCs are open even after depletion of the Ca2+ store in th...

    journal_title:Journal of cell science

    pub_type: 评论,杂志文章,评审

    doi:10.1242/jcs.01295

    authors: Gerasimenko O,Gerasimenko J

    更新日期:2004-07-01 00:00:00

  • Ultrastructure of the dinoflagellate Polykrikos. I. Development of the nematocyst-taeniocyst complex and morphology of the site for extrusion.

    abstract::Development of the nematocyst-taeniocyst complex in the four-zooid stage of a dinoflagellate, Polykrikos kofoidi, was studied by electron microscopy. We observed the following stages: formation of large spherical bodies in islets of cytoplasm containing extensive rough endoplasmic reticulum and Golgi complexes; differ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Westfall JA,Bradbury PC,Townsend JW

    更新日期:1983-09-01 00:00:00

  • Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusion.

    abstract::The process of regulated exocytosis is defined by the Ca2+-triggered fusion of two apposed membranes, enabling the release of vesicular contents. This fusion step involves a number of energetically complex steps and requires both protein and lipid membrane components. The role of cholesterol has been investigated usin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02601

    authors: Churchward MA,Rogasevskaia T,Höfgen J,Bau J,Coorssen JR

    更新日期:2005-10-15 00:00:00

  • High molecular weight tau: preferential localization in the peripheral nervous system.

    abstract::Using epitope mapping we have demonstrated that a high molecular weight protein (Mr approximately 115 x 10(3)) present in brain and spinal cord is a member of the tau family of microtubule-associated proteins. Antibodies directed against the amino-terminal, middle and carboxyl-terminal portions of tau recognize this p...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Georgieff IS,Liem RK,Mellado W,Nunez J,Shelanski ML

    更新日期:1991-09-01 00:00:00