Abstract:
:Protein degradation in chloroplasts is carried out by a set of proteases that eliminate misfolded, damaged, or superfluous proteins. The ATP-dependent caseinolytic protease (Clp) is the most complex protease in plastids and has been implicated mainly in stromal protein degradation. In contrast, FtsH, a thylakoid membrane-associated metalloprotease, is believed to participate mainly in the degradation of thylakoidal proteins. To determine the role of specific Clp and FtsH subunits in plant growth and development, RNAi lines targeting at least one subunit of each Clp ring and FtsH were generated in tobacco. In addition, mutation of the translation initiation codon was employed to down-regulate expression of the plastid-encoded ClpP1 subunit. These protease lines cover a broad range of reductions at the transcript and protein levels of the targeted genes. A wide spectrum of phenotypes was obtained, including pigment deficiency, alterations in leaf development, leaf variegations, and impaired photosynthesis. When knock-down lines for the different protease subunits were compared, both common and specific phenotypes were observed, suggesting distinct functions of at least some subunits. Our work provides a well-characterized collection of knock-down lines for plastid proteases in tobacco and reveals the importance of the Clp protease in physiology and plant development.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Moreno JC,Tiller N,Diez M,Karcher D,Tillich M,Schöttler MA,Bock Rdoi
10.1093/jxb/erx066subject
Has Abstractpub_date
2017-04-01 00:00:00pages
2199-2218issue
9eissn
0022-0957issn
1460-2431pii
3091865journal_volume
68pub_type
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journal_title:Journal of experimental botany
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abstract::The aleurone layer of cereal grains is important biologically as well as nutritionally and economically. Here, current knowledge on the regulation of aleurone development is reviewed. Recent reports suggest that the control of aleurone development is more complex than earlier models portrayed. Multiple levels of genet...
journal_title:Journal of experimental botany
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pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00
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journal_title:Journal of experimental botany
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更新日期:2016-03-01 00:00:00
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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doi:
更新日期:2002-06-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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