Abstract:
BACKGROUND:Molecular techniques are relevant to modern cytopathology, but their implementation is difficult without molecular expertise and infrastructure. The assessment of KRAS mutational status on cytological preparations may be useful either to refine uncertain diagnoses on pancreatic aspirates or to yield predictive information to plan targeted treatment of metastatic colorectal cancer (mCRC). The novel test Idylla™ enables fully automated KRAS genotyping in approximately 2 h, even in less experienced hands. MATERIALS AND METHODS:This study aims to validate this methodology to detect KRAS mutations on archival cytological preparations of pancreatic cancer (n = 9) and mCRC (n = 9) by comparing the Idylla™ performance to that of standard real-time polymerase chain reaction. RESULTS:The same 11 mutations (n = 4: p.G12D; n = 2: p.G12V; n = 2: p.A59E/G/T; n = 1: p.G12R; n = 1: p.G13D; n = 1: p.Q61H) were detected by both techniques. CONCLUSION:Even in less experienced laboratories, a cytopathologist may easily integrate morphological diagnostic report with accurate KRAS mutation detection, which is relevant for diagnostic and treatment decisions.
journal_name
Cytojournaljournal_title
CytoJournalauthors
De Luca C,Vigliar E,d'Anna M,Pisapia P,Bellevicine C,Malapelle U,Troncone Gdoi
10.4103/1742-6413.200936subject
Has Abstractpub_date
2017-02-24 00:00:00pages
5eissn
0974-5963issn
1742-6413pii
CJ-14-5journal_volume
14pub_type
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