Automated Protein Biomarker Analysis: on-line extraction of clinical samples by Molecularly Imprinted Polymers.

Abstract:

:Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Rossetti C,Świtnicka-Plak MA,Grønhaug Halvorsen T,Cormack PA,Sellergren B,Reubsaet L

doi

10.1038/srep44298

subject

Has Abstract

pub_date

2017-03-17 00:00:00

pages

44298

issn

2045-2322

pii

srep44298

journal_volume

7

pub_type

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