Abstract：
OBJECTIVE:MicroRNA-1254 (miR-1254) has not been studied in colorectal cancer (CRC) to date. This study aimed to investigate the inhibitory mechanism of miR-1254 in CRC tumorigenesis. METHODS:MiR-1254 expression was examined using real-time polymerase chain reaction in CRC and adjacent non-tumorous tissues. The correlation between miR-1254 expressions and proliferation and migration of cancer cells was determined using the CCK-8 and transwell assays. RNA sequencing was used to identify differentially expressed genes downstream from miR-1254. A luciferase reporter assay was used to confirm the direct interaction between miR-1254 and its predicted target gene, PSMD10. Moreover, PSMD10 was either overexpressed or silenced in colon carcinoma cells overexpressing miR-1254 to determine whether their interaction contributed to CRC migration and epithelial-mesenchymal transition (EMT). RESULTS:Significantly lower miR-1254 expressions were observed in CRC tissues than in adjacent non-tumorous tissues. Exogenous miR-1254 expression suppressed the migration of colon carcinoma cell lines SW1116 and HCT116. RNA sequencing and luciferase assays revealed that miR-1254 directly binded to the 3'-untranslated region of PSMD10, an important regulator of EMT and cell migration. PSMD10 knockdown inhibited EMT and colon cancer cell migration, whereas PSMD10 overexpression reversed the inhibition of EMT and cell migration caused by miR-1254. CONCLUSION:MiR-1254 may act as a tumor suppressor in CRC and may inhibit CRC migration by directly targeting PSMD10 to suppress the EMT process.
journal_name
J Dig Disjournal_title
Journal of digestive diseasesauthors
Chu YM,Peng HX,Xu Y,Yang DM,Zhou FL,Li J,Kuai R,Lin Ydoi
10.1111/1751-2980.12463subject
Has Abstractpub_date
2017-03-01 00:00:00pages
169-178issue
3eissn
1751-2972issn
1751-2980journal_volume
18pub_type
杂志文章abstract：OBJECTIVES:Preoperative biliary drainage (PBD) can relieve symptoms of cholestasis, but carries risk of procedural complications. Metal stents have wider lumens and longer patency, although plastic stents (PS) remain in use. We reviewed the outcomes after PBD in patients with cholestasis. METHODS:Patients with symptom...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12455
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:We aimed to identify the differences in symptoms, high-resolution manometry (HRM) characteristics, and the efficacy of peroral endoscopic myotomy (POEM) regarding patients' sex in achalasia. METHODS:All patients diagnosed with achalasia by HRM who underwent POEM and were followed up for more than 6 months we...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12922
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:It is known that the vacuolating cytotoxin (VacA) could induce apoptosis. However, the mechanism remained to be elucidated. The aim of this study is to investigate the role of Bcl family of proteins (Bcl-2 and Bax) and the mitochondrial voltage-dependent anion channel (VDAC) in VacA-induced apoptosis of AGS c...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2009.00412.x
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVES:To assess the prevalence and potential risk factors of latent tuberculosis infection (LTBI) in Chinese patients with inflammatory bowel disease (IBD) and to evaluate the role of chest computed tomography (CT) in the screening of LTBI. METHODS:A single-center retrospective study was conducted and all IBD pat...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12437
更新日期：2017-01-01 00:00:00
abstract：:The incidence of early esophageal adenocarcinoma has been increasing significantly in recent decades. Prognosis depends greatly on the choice of treatment. Early cancers can be treated by endoscopic resection, whereas advanced carcinomas have to be sent for surgery. Esophageal resection is associated with high periope...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12264
更新日期：2015-07-01 00:00:00
abstract：:Fibroblast infiltration and collagen deposition result in structural changes in the bowel wall, and lead to strictures in intestinal inflammatory disease. While strictures can also occur in other contexts, such as malignancy, this review focuses on the surgical treatment of stricture secondary to inflammatory bowel di...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12880
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:Systematic reports on acute upper gastrointestinal bleeding in children/adolescents are scanty. The aim of this study is to analyze its presentation, pathology and outcome in Hong Kong. METHODS:A retrospective review of the hospital database for admissions up to the age of 18 years with signs of acute upper ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2008.00346.x
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:This study aimed to investigate the efficacy of fluorescence spectroscopy of gastric juice for early gastric cancer (EGC) screening. METHODS:Gastric juice was collected from 101 participants who underwent endoscopy in the Outpatient Endoscopy Center of Peking University Third Hospital. The participants were ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12040
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To investigate the role of ghrelin in the gastric cancer cell line AGS and its probable mechanism. METHODS:Cell proliferation was detected by MTT assay after treated with ghrelin or des-acyl ghrelin. The expression of growth hormone secretagogue receptor 1a (GHS-R1a) and 1b (GHS-R1b) mRNA was detected using ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2012.00616.x
更新日期：2012-09-01 00:00:00
abstract：:Acute non-variceal upper gastrointestinal bleeding (ANVUGIB) is one of the most common medical emergencies in China and worldwide. In 2009, we published the "Guidelines for the diagnosis and treatment of acute non-variceal upper gastrointestinal bleeding" for the patients in China; however, during the past years numer...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,实务指引
doi：10.1111/1751-2980.12319
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:To investigate the expression of peroxisome proliferator-activated receptors (PPAR)gamma and the effects of PPARgamma ligands on cells growth in colorectal cancer (CRC) cell line HT-29, and to explore whether the activation of PPARgamma by its selective ligand can induce apoptosis and the arrest of the cell c...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1443-9573.2007.00290.x
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:We conducted a randomized trial aiming at improving patients' informed consent for undergoing endoscopic retrograde cholangiopancreatography (ERCP) in clinical care by comparing the efficacy of an additional educational video to written informed consent with that of written informed consent alone. METHODS:Th...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,随机对照试验
doi：10.1111/1751-2980.12824
更新日期：2019-12-01 00:00:00
abstract：:The development of alcoholic liver disease (ALD) is a complex process involving both the parenchymal and non-parenchymal cells in the liver. The impact of ethanol on hepatocytes can be characterized as a condition of organelle stress with multifactorial changes in hepatocellular function accumulating during ethanol ex...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2010.00468.x
更新日期：2011-02-01 00:00:00
abstract：:Helicobacter pylori (H. pylori) infection causes peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphomas and gastric adenocarcinomas, for which the pathogenesis of chronic gastric inflammation prevails and provides the pathogenic basis. Since the role of H. pylori infection is promoting carcinogenesis...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2008.00334.x
更新日期：2008-08-01 00:00:00
abstract：OBJECTIVE:Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, the natural course of which has not been well documented. This study aimed to perform a prospective cohort study to investigate NAFLD in a Chinese population. METHODS:Using our previous epidemiological survey, 3543 patients were foll...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2011.00571.x
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:Contrast-enhanced harmonic endoscopic ultrasonography (CH-EUS) is a valuable device to diagnose and determine the malignant potential of gastrointestinal stromal tumors (GIST) as early as possible when making clinical therapeutic decisions. This study aimed to estimate the ability of CH-EUS to discriminate be...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,meta分析
doi：10.1111/1751-2980.12710
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of the non-cytotoxic drug (cyclooxygenase-2 (COX-2) inhibitor and octreotide) on growth of the multidrug-resistant human gastric carcinoma cell line SGC-7901/ADR. METHODS:The effects of non-cytotoxic drug on the growth of SGC-7901 and SGC-7901/ADR cells were evaluated by (3)H-thymi...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2009.00370.x
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:The incidence of nonsteroidal anti-inflammatory drugs (NSAIDs)-induced enteropathy is currently increasing. However, the predictors of small bowel bleeding (SBB) associated with NSAIDs are unknown. This study aimed to assess the risk factors of SBB in chronic NSAIDs users. METHODS:We retrospectively compared...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12269
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the role of insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) in the development of hepatic fibrogenesis in experimental disease models and human liver samples. METHODS:Cellular distribution patterns of IGFBP-rP1 were assessed by immunohistochem...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12126
更新日期：2014-04-01 00:00:00
abstract：:Lgr5 marks stem cells in digestive epithelial tissues by lineage tracing, and in vitro ever-expansion of Lgr5 stem cells form organoids, which can be directed to differentiate into functional somatic cells. Organoids derived from gastrointestinal epithelium even recapitulate the morphologic features of their in vivo c...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12305
更新日期：2016-01-01 00:00:00
abstract：:Non-alcoholic fatty liver disease (NAFLD) is now the most common etiology of chronic liver disease threatening global public health. However, the name "NAFLD" is no longer appropriate with the change of time. Recently, a new term, "metabolic dysfunction-associated fatty liver disease" has been proposed by an internati...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12944
更新日期：2020-11-01 00:00:00
abstract：OBJECTIVE:To evaluate whether patients with malignant biliary obstruction (MBO) benefit from balloon dilation before the placement of a self-expanding metal stent (SEMS) for palliative biliary drainage. METHODS:Consecutive patients who underwent endoscopic retrograde cholangiopancreatography with SEMS placement for pa...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12864
更新日期：2020-05-01 00:00:00
abstract：:Barrett's esophagus (BE)-related esophageal adenocarcinoma (EAC) has shown the fastest rise in incidence in Western countries; however, research data on BE-related diseases from China are inconclusive. We aimed to review and analyze the published results on these diseases in China. We searched PubMed and Chinese medic...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2011.00535.x
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the role of endoscopic drainage and endoscopic necrosectomy in the management of symptomatic pancreatic fluid collection. METHODS:The clinical data of patients with symptomatic pancreatic fluid collection referred for endoscopic drainage were captured prospectively and analyzed. Pancreatic duct d...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2009.00388.x
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:Currently the screening and diagnosis of gastric cancer and atrophic gastritis are mainly made by endoscopy and biopsy. The aim of this study was to evaluate the use of serum tests: serum pepsinogen I (PGI pepsinogen I/II ratio (PGR), gastrin-17 (G-17) and H. pylori-immunoglobulin G (IgG) antibodies to screen...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1443-9573.2007.00271.x
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVE:Early diagnosis of colitis-associated cancer and dysplasia through surveillance endoscopy is vital for patients with ulcerative colitis (UC). This study aimed to evaluate the efficacy of autofluorescence endoscopy (AFE) using 5-aminolevulinic acid (ALA) and to investigate the fluorescence signal localization ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12923
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:We aimed to investigate the relationship between the number of involved organs or regions and serum immunoglobulin G (IgG) and immunoglobulin G4 (IgG4) levels. METHODS:The number of pancreatic and/or extrapancreatic lesions and serum IgG and IgG4 levels were examined by groups in 46 patients with IgG4-relate...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2012.00583.x
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:This study aimed to compare the diagnostic value and image quality of high-definition magnifying white light endoscopy (M-WLE) and high-definition magnifying endoscopy with i-scan (M-i-scan) for Helicobacter pylori (H.pylori) infection. METHODS:Patients complaining of dyspepsia who underwent esophagogastrodu...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12086
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:To investigate whether sending a family history questionnaire to patients prior to undergoing colonoscopy results in an increased availability of family history and better genetic counseling. METHODS:A questionnaire was mailed to patients before they underwent outpatient colonoscopy at a university hospital...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12491
更新日期：2017-06-01 00:00:00
abstract：:Drug-induced liver injury (DILI), which is caused by drugs and herbal or dietary supplements, remains a serious concern for drug developers, regulators, and clinicians; however, serum biomarkers utilized to detect and monitor DILI have not changed in decades and have limitations. Data-driven mathematical modeling that...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12684
更新日期：2019-01-01 00:00:00
