Abstract：
:A millisecond micro-RNA separation of a mixture of total RNA and genomic DNA, extracted from cultured HeLa cells, was successfully achieved using a hybrid structure of nanopillars and nanoslits contained inside a microchannel. The nanopillars, 250-nm in diameter and 100-nm in height, were fabricated with a 750-nm space inside the nanoslits, which were 100-nm in height and 25-μm in width; the nanopillars were then applied as a new sieve matrix. This ultra-fast technique for the separation of miRNA can be an effective pretreatment for semiconductor nanopore DNA sequencing, which has an optimum reading speed of 1 base/ms to obtain effective signal-to-noise ratio and discriminate each base by ion or tunneling current during the passage of nucleic acids.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu Q,Kaji N,Yasui T,Rahong S,Yanagida T,Kanai M,Nagashima K,Tokeshi M,Kawai T,Baba Ydoi
10.1038/srep43877subject
Has Abstractpub_date
2017-03-08 00:00:00pages
43877issn
2045-2322pii
srep43877journal_volume
7pub_type
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