Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.

Abstract:

:How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

doi

10.1016/j.devcel.2017.01.015

subject

Has Abstract

pub_date

2017-02-27 00:00:00

pages

331-341.e4

issue

4

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(17)30042-4

journal_volume

40

pub_type

杂志文章
  • Cleavage of TRPM7 releases the kinase domain from the ion channel and regulates its participation in Fas-induced apoptosis.

    abstract::Transient receptor potential melastatin-like 7 (TRPM7) is a channel protein that also contains a regulatory serine-threonine kinase domain. Here, we find that Trpm7-/- T cells are deficient in Fas-receptor-induced apoptosis and that TRPM7 channel activity participates in the apoptotic process and is regulated by caspa...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.04.006

    authors: Desai BN,Krapivinsky G,Navarro B,Krapivinsky L,Carter BC,Febvay S,Delling M,Penumaka A,Ramsey IS,Manasian Y,Clapham DE

    更新日期:2012-06-12 00:00:00

  • Role of mitochondrial remodeling in programmed cell death in Drosophila melanogaster.

    abstract::The role of mitochondria in Drosophila programmed cell death remains unclear, although certain gene products that regulate cell death seem to be evolutionarily conserved. We find that developmental programmed cell death stimuli in vivo and multiple apoptotic stimuli ex vivo induce dramatic mitochondrial fragmentation ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2007.02.002

    authors: Goyal G,Fell B,Sarin A,Youle RJ,Sriram V

    更新日期:2007-05-01 00:00:00

  • Ectodermal Smad4 and p38 MAPK are functionally redundant in mediating TGF-beta/BMP signaling during tooth and palate development.

    abstract::Smad4 is a central intracellular effector of TGF-beta signaling. Smad-independent TGF-beta pathways, such as those mediated by p38 MAPK, have been identified in cell culture systems, but their in vivo functional mechanisms remain unclear. In this study, we investigated the role of TGF-beta signaling in tooth and palat...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2008.06.004

    authors: Xu X,Han J,Ito Y,Bringas P Jr,Deng C,Chai Y

    更新日期:2008-08-01 00:00:00

  • GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

    abstract::By functioning as an enzyme cofactor, hemoglobin component, and gene regulator, heme is vital for life. One mode of heme-regulated transcription involves amplifying the activity of GATA-1, a key determinant of erythrocyte differentiation. To discover biological consequences of the metal cofactor-transcription factor m...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2018.07.022

    authors: Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

    更新日期:2018-09-10 00:00:00

  • Nucleosome-depleted regions in cell-cycle-regulated promoters ensure reliable gene expression in every cell cycle.

    abstract::Many promoters in eukaryotes have nucleosome-depleted regions (NDRs) containing transcription factor binding sites. However, the functional significance of NDRs is not well understood. Here, we examine NDR function in two cell cycle-regulated promoters, CLN2pr and HOpr, by varying nucleosomal coverage of the binding s...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2010.02.007

    authors: Bai L,Charvin G,Siggia ED,Cross FR

    更新日期:2010-04-20 00:00:00

  • SAG/RBX2/ROC2 E3 ubiquitin ligase is essential for vascular and neural development by targeting NF1 for degradation.

    abstract::SAG/RBX2/ROC2 protein is an essential RING component of SCF E3 ubiquitin ligase. The role of SAG during embryogenesis remains unknown. We report a critical role for SAG in controlling vascular and neural development by modulating RAS activity via promoting degradation of neurofibromatosis type 1 (NF1). Mice mutant for...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.09.014

    authors: Tan M,Zhao Y,Kim SJ,Liu M,Jia L,Saunders TL,Zhu Y,Sun Y

    更新日期:2011-12-13 00:00:00

  • Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.

    abstract::Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiati...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.02.012

    authors: Eritano AS,Bromley CL,Bolea Albero A,Schütz L,Wen FL,Takeda M,Fukaya T,Sami MM,Shibata T,Lemke S,Wang YC

    更新日期:2020-04-20 00:00:00

  • Intrinsic tumor suppression and epithelial maintenance by endocytic activation of Eiger/TNF signaling in Drosophila.

    abstract::Oncogenic alterations in epithelial tissues often trigger apoptosis, suggesting an evolutionary mechanism by which organisms eliminate aberrant cells from epithelia. In Drosophila imaginal epithelia, clones of cells mutant for tumor suppressors, such as scrib or dlg, lose their polarity and are eliminated by cell deat...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2009.01.002

    authors: Igaki T,Pastor-Pareja JC,Aonuma H,Miura M,Xu T

    更新日期:2009-03-01 00:00:00

  • Meiosis researchers exchange information in the Alps.

    abstract::The 6th European Meiosis Workshop, sponsored by EMBO and organized by Franz Klein of the University of Vienna, met in mid-September. More than 150 participants who have not discovered anything more interesting than sex gathered in a bucolic Alpine valley near the town of Obertraun, Austria, surrounded by granite massi...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(03)00336-8

    authors: Dernburg AF

    更新日期:2003-11-01 00:00:00

  • Rho Flares Repair Local Tight Junction Leaks.

    abstract::Tight junctions contribute to epithelial barrier function by selectively regulating the quantity and type of molecules that cross the paracellular barrier. Experimental approaches to evaluate the effectiveness of tight junctions are typically global, tissue-scale measures. Here, we introduce Zinc-based Ultrasensitive ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2019.01.016

    authors: Stephenson RE,Higashi T,Erofeev IS,Arnold TR,Leda M,Goryachev AB,Miller AL

    更新日期:2019-02-25 00:00:00

  • 3' UTRs in the Eye of the TIGER.

    abstract::Many human genes generate mRNAs with alternative 3' untranslated regions (3' UTRs), which can modulate protein function. In a recent Cell paper, Ma and Mayr (2018) unveil a major membraneless organelle, termed the TIS granule-endoplasmic reticulum (TIGER), which enables specific 3' UTRs to encipher protein localizatio...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2018.11.022

    authors: Portz B,Shorter J

    更新日期:2018-12-03 00:00:00

  • The grapes of incompatibility.

    abstract::When two colonies of the tunicate Botryllus contact each other, they either fuse into a single colony or mount a destructive reaction, which keeps them apart. Which of the two reactions takes place is determined by a single, highly polymorphic fusion/histocompatibility (FuHC) locus. Recent communications report the cl...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.12.009

    authors: Klein J

    更新日期:2006-01-01 00:00:00

  • SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion.

    abstract::Migrating fibroblasts undergo contact inhibition of locomotion (CIL), a process that was discovered five decades ago and still is not fully understood at the molecular level. We identify the Slit2-Robo4-srGAP2 signaling network as a key regulator of CIL in fibroblasts. CIL involves highly dynamic contact protrusions w...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.09.002

    authors: Fritz RD,Menshykau D,Martin K,Reimann A,Pontelli V,Pertz O

    更新日期:2015-10-12 00:00:00

  • How the sea squirt nucleus tells mesoderm not to be endoderm.

    abstract::Sea squirts are simple invertebrate chordates. In this issue of Developmental Cell, Takatori et al. show nuclear migration within ascidian mesendodermal cells enables polarized localization of Not mRNA, which encodes a homeobox protein that distinguishes mesoderm from endoderm fates. The link between nuclear migration...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2010.10.002

    authors: Parton RM,Davis I

    更新日期:2010-10-19 00:00:00

  • Temporal and spatial regulation of phosphoinositide signaling mediates cytokinesis.

    abstract::Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.02.010

    authors: Janetopoulos C,Borleis J,Vazquez F,Iijima M,Devreotes P

    更新日期:2005-04-01 00:00:00

  • A ubiquitin-binding rhomboid protease aimed at ERADication.

    abstract::Proteins are degraded from the ER by endoplasmic reticulum-associated degradation (ERAD). In a recent issue of Molecular Cell, Fleig et al. (2012) describe a role for a ubiquitin-binding rhomboid protease, RHBDL4, in degradation of select ERAD substrates. These findings and the significance of rhomboids and other intr...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2012.08.015

    authors: Tsai YC,Weissman AM

    更新日期:2012-09-11 00:00:00

  • PREEParing for mitosis.

    abstract::Reporting in Developmental Cell, Schlaitz et al. (2013) show that endoplasmic reticulum (ER) membrane exclusion from the mitotic spindle is an active process requiring REEP membrane proteins. REEP protein depletion results in ER membrane retention on the spindle and chromosomes, leading to defects in chromosome segreg...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2013.07.018

    authors: Burke B

    更新日期:2013-08-12 00:00:00

  • Cellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell imaging.

    abstract::Border cells in the Drosophila ovary originate within an epithelium, detach from it, invade neighboring nurse cells, and migrate as a coherent cluster. This migration has served as a useful genetic model for understanding epithelial cell motility. The prevailing model of growth factor-mediated chemotaxis in general, a...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2007.03.021

    authors: Prasad M,Montell DJ

    更新日期:2007-06-01 00:00:00

  • PRC2-Associated Chromatin Contacts in the Developing Limb Reveal a Possible Mechanism for the Atypical Role of PRC2 in HoxA Gene Expression.

    abstract::Preventing inappropriate gene expression in time and space is as fundamental as triggering the activation of tissue- or cell-type-specific factors at the correct developmental stage and in the correct cells. Here, we study the impact of Polycomb repressive complex 2 (PRC2) function on HoxA gene regulation. We analyze ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2019.05.021

    authors: Gentile C,Berlivet S,Mayran A,Paquette D,Guerard-Millet F,Bajon E,Dostie J,Kmita M

    更新日期:2019-07-22 00:00:00

  • Accessing the Phenotype Gap: Enabling Systematic Investigation of Paralog Functional Complexity with CRISPR.

    abstract::Single-gene knockout experiments can fail to reveal function in the context of redundancy, which is frequently observed among duplicated genes (paralogs) with overlapping functions. We discuss the complexity associated with studying paralogs and outline how recent advances in CRISPR will help address the "phenotype ga...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2017.09.020

    authors: Ewen-Campen B,Mohr SE,Hu Y,Perrimon N

    更新日期:2017-10-09 00:00:00

  • Regulation of angiogenesis by oxygen and metabolism.

    abstract::Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2009.01.003

    authors: Fraisl P,Mazzone M,Schmidt T,Carmeliet P

    更新日期:2009-02-01 00:00:00

  • Spindle formation in the mouse embryo requires Plk4 in the absence of centrioles.

    abstract::During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2013.09.029

    authors: Coelho PA,Bury L,Sharif B,Riparbelli MG,Fu J,Callaini G,Glover DM,Zernicka-Goetz M

    更新日期:2013-12-09 00:00:00

  • The transitional ER localization mechanism of Pichia pastoris Sec12.

    abstract::COPII vesicles assemble at ER subdomains called transitional ER (tER) sites, but the mechanism that generates tER sites is unknown. To study tER biogenesis, we analyzed the transmembrane protein Sec12, which initiates COPII vesicle formation. Sec12 is concentrated at discrete tER sites in the budding yeast Pichia past...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(04)00129-7

    authors: Soderholm J,Bhattacharyya D,Strongin D,Markovitz V,Connerly PL,Reinke CA,Glick BS

    更新日期:2004-05-01 00:00:00

  • CNS myelin wrapping is driven by actin disassembly.

    abstract::Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.06.011

    authors: Zuchero JB,Fu MM,Sloan SA,Ibrahim A,Olson A,Zaremba A,Dugas JC,Wienbar S,Caprariello AV,Kantor C,Leonoudakis D,Lariosa-Willingham K,Kronenberg G,Gertz K,Soderling SH,Miller RH,Barres BA

    更新日期:2015-07-27 00:00:00

  • N-WASP Guides Cancer Cells toward LPA.

    abstract::The actin remodeling factor N-WASP is best known as an Arp2/3 complex activator in processes like endocytosis, extracellular matrix degradation, and host-pathogen interaction. In this issue of Developmental Cell, Juin et al. establish a novel trafficking function for N-WASP in driving lysophosphatidic acid-dependent c...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.10.029

    authors: Rottner K,Schaks M

    更新日期:2019-11-18 00:00:00

  • Bud building by septin patch hole punching.

    abstract::Small GTPase Cdc42 triggers polarity establishment in budding yeast. In this issue of Developmental Cell, Okada et al. (2013) combine in silico modeling and cell biology to show that Cdc42, septins, and the exocytosis pathway are integrated in a feedback system to define and insulate the site of polarity in the membra...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2013.07.004

    authors: Caudron F,Barral Y

    更新日期:2013-07-29 00:00:00

  • Signaling from Glia and Cholinergic Neurons Controls Nutrient-Dependent Production of an Insulin-like Peptide for Drosophila Body Growth.

    abstract::The insulin-like peptide (ILP) family plays key biological roles in the control of body growth. Although the functions of ILPs are well understood, the mechanisms by which organisms sense their nutrient status and thereby control ILP production remain largely unknown. Here, we show that signaling relay and feedback me...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2015.10.003

    authors: Okamoto N,Nishimura T

    更新日期:2015-11-09 00:00:00

  • Nuclear Deformation Lets Cells Gauge Their Physical Confinement.

    abstract::How cells sense their physical microenvironment remains incompletely understood. In two recent Science articles, Lomakin et al. (2020) and Venturini et al. (2020) demonstrate that progressive nuclear deformation associated with cellular confinement triggers intracellular events that promote cell contractility and migr...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2021.01.002

    authors: Long JT,Lammerding J

    更新日期:2021-01-25 00:00:00

  • GTP hydrolysis by the Rho family GTPase TC10 promotes exocytic vesicle fusion.

    abstract::TC10, a Rho family GTPase, has been shown to play an important role in the exocytosis of GLUT4 and other proteins, primarily by tethering the vesicles at the plasma membrane. Using a newly developed probe based on fluorescence resonance energy transfer, we found that TC10 activity at tethered vesicles dropped immediat...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2006.07.008

    authors: Kawase K,Nakamura T,Takaya A,Aoki K,Namikawa K,Kiyama H,Inagaki S,Takemoto H,Saltiel AR,Matsuda M

    更新日期:2006-09-01 00:00:00

  • SnoN Antagonizes the Hippo Kinase Complex to Promote TAZ Signaling during Breast Carcinogenesis.

    abstract::SnoN regulates multiple signaling pathways, including TGF-β/Smad and p53, and displays both pro-oncogenic and anti-oncogenic activities in human cancer. We have observed previously that both its intracellular localization and expression levels are sensitive to cell density, suggesting that it may crosstalk with Hippo ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2016.05.002

    authors: Zhu Q,Le Scolan E,Jahchan N,Ji X,Xu A,Luo K

    更新日期:2016-06-06 00:00:00