Abstract:
:How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.
journal_name
Dev Celljournal_title
Developmental cellauthors
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla Adoi
10.1016/j.devcel.2017.01.015subject
Has Abstractpub_date
2017-02-27 00:00:00pages
331-341.e4issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(17)30042-4journal_volume
40pub_type
杂志文章abstract::Transient receptor potential melastatin-like 7 (TRPM7) is a channel protein that also contains a regulatory serine-threonine kinase domain. Here, we find that Trpm7-/- T cells are deficient in Fas-receptor-induced apoptosis and that TRPM7 channel activity participates in the apoptotic process and is regulated by caspa...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.04.006
更新日期:2012-06-12 00:00:00
abstract::The role of mitochondria in Drosophila programmed cell death remains unclear, although certain gene products that regulate cell death seem to be evolutionarily conserved. We find that developmental programmed cell death stimuli in vivo and multiple apoptotic stimuli ex vivo induce dramatic mitochondrial fragmentation ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.02.002
更新日期:2007-05-01 00:00:00
abstract::Smad4 is a central intracellular effector of TGF-beta signaling. Smad-independent TGF-beta pathways, such as those mediated by p38 MAPK, have been identified in cell culture systems, but their in vivo functional mechanisms remain unclear. In this study, we investigated the role of TGF-beta signaling in tooth and palat...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.06.004
更新日期:2008-08-01 00:00:00
abstract::By functioning as an enzyme cofactor, hemoglobin component, and gene regulator, heme is vital for life. One mode of heme-regulated transcription involves amplifying the activity of GATA-1, a key determinant of erythrocyte differentiation. To discover biological consequences of the metal cofactor-transcription factor m...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.07.022
更新日期:2018-09-10 00:00:00
abstract::Many promoters in eukaryotes have nucleosome-depleted regions (NDRs) containing transcription factor binding sites. However, the functional significance of NDRs is not well understood. Here, we examine NDR function in two cell cycle-regulated promoters, CLN2pr and HOpr, by varying nucleosomal coverage of the binding s...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2010.02.007
更新日期:2010-04-20 00:00:00
abstract::SAG/RBX2/ROC2 protein is an essential RING component of SCF E3 ubiquitin ligase. The role of SAG during embryogenesis remains unknown. We report a critical role for SAG in controlling vascular and neural development by modulating RAS activity via promoting degradation of neurofibromatosis type 1 (NF1). Mice mutant for...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.09.014
更新日期:2011-12-13 00:00:00
abstract::Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiati...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.02.012
更新日期:2020-04-20 00:00:00
abstract::Oncogenic alterations in epithelial tissues often trigger apoptosis, suggesting an evolutionary mechanism by which organisms eliminate aberrant cells from epithelia. In Drosophila imaginal epithelia, clones of cells mutant for tumor suppressors, such as scrib or dlg, lose their polarity and are eliminated by cell deat...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2009.01.002
更新日期:2009-03-01 00:00:00
abstract::The 6th European Meiosis Workshop, sponsored by EMBO and organized by Franz Klein of the University of Vienna, met in mid-September. More than 150 participants who have not discovered anything more interesting than sex gathered in a bucolic Alpine valley near the town of Obertraun, Austria, surrounded by granite massi...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00336-8
更新日期:2003-11-01 00:00:00
abstract::Tight junctions contribute to epithelial barrier function by selectively regulating the quantity and type of molecules that cross the paracellular barrier. Experimental approaches to evaluate the effectiveness of tight junctions are typically global, tissue-scale measures. Here, we introduce Zinc-based Ultrasensitive ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2019.01.016
更新日期:2019-02-25 00:00:00
abstract::Many human genes generate mRNAs with alternative 3' untranslated regions (3' UTRs), which can modulate protein function. In a recent Cell paper, Ma and Mayr (2018) unveil a major membraneless organelle, termed the TIS granule-endoplasmic reticulum (TIGER), which enables specific 3' UTRs to encipher protein localizatio...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.11.022
更新日期:2018-12-03 00:00:00
abstract::When two colonies of the tunicate Botryllus contact each other, they either fuse into a single colony or mount a destructive reaction, which keeps them apart. Which of the two reactions takes place is determined by a single, highly polymorphic fusion/histocompatibility (FuHC) locus. Recent communications report the cl...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.12.009
更新日期:2006-01-01 00:00:00
abstract::Migrating fibroblasts undergo contact inhibition of locomotion (CIL), a process that was discovered five decades ago and still is not fully understood at the molecular level. We identify the Slit2-Robo4-srGAP2 signaling network as a key regulator of CIL in fibroblasts. CIL involves highly dynamic contact protrusions w...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.09.002
更新日期:2015-10-12 00:00:00
abstract::Sea squirts are simple invertebrate chordates. In this issue of Developmental Cell, Takatori et al. show nuclear migration within ascidian mesendodermal cells enables polarized localization of Not mRNA, which encodes a homeobox protein that distinguishes mesoderm from endoderm fates. The link between nuclear migration...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2010.10.002
更新日期:2010-10-19 00:00:00
abstract::Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.02.010
更新日期:2005-04-01 00:00:00
abstract::Proteins are degraded from the ER by endoplasmic reticulum-associated degradation (ERAD). In a recent issue of Molecular Cell, Fleig et al. (2012) describe a role for a ubiquitin-binding rhomboid protease, RHBDL4, in degradation of select ERAD substrates. These findings and the significance of rhomboids and other intr...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2012.08.015
更新日期:2012-09-11 00:00:00
abstract::Reporting in Developmental Cell, Schlaitz et al. (2013) show that endoplasmic reticulum (ER) membrane exclusion from the mitotic spindle is an active process requiring REEP membrane proteins. REEP protein depletion results in ER membrane retention on the spindle and chromosomes, leading to defects in chromosome segreg...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2013.07.018
更新日期:2013-08-12 00:00:00
abstract::Border cells in the Drosophila ovary originate within an epithelium, detach from it, invade neighboring nurse cells, and migrate as a coherent cluster. This migration has served as a useful genetic model for understanding epithelial cell motility. The prevailing model of growth factor-mediated chemotaxis in general, a...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2007.03.021
更新日期:2007-06-01 00:00:00
abstract::Preventing inappropriate gene expression in time and space is as fundamental as triggering the activation of tissue- or cell-type-specific factors at the correct developmental stage and in the correct cells. Here, we study the impact of Polycomb repressive complex 2 (PRC2) function on HoxA gene regulation. We analyze ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2019.05.021
更新日期:2019-07-22 00:00:00
abstract::Single-gene knockout experiments can fail to reveal function in the context of redundancy, which is frequently observed among duplicated genes (paralogs) with overlapping functions. We discuss the complexity associated with studying paralogs and outline how recent advances in CRISPR will help address the "phenotype ga...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2017.09.020
更新日期:2017-10-09 00:00:00
abstract::Blood vessels form an important interface between the environment and the organism by carrying oxygen and nutrients to all cells and thus determining cellular metabolism. It is therefore not surprising that oxygen and metabolism influence the development of the vascular network. Here, we discuss recent insights regard...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2009.01.003
更新日期:2009-02-01 00:00:00
abstract::During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2013.09.029
更新日期:2013-12-09 00:00:00
abstract::COPII vesicles assemble at ER subdomains called transitional ER (tER) sites, but the mechanism that generates tER sites is unknown. To study tER biogenesis, we analyzed the transmembrane protein Sec12, which initiates COPII vesicle formation. Sec12 is concentrated at discrete tER sites in the budding yeast Pichia past...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(04)00129-7
更新日期:2004-05-01 00:00:00
abstract::Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.06.011
更新日期:2015-07-27 00:00:00
abstract::The actin remodeling factor N-WASP is best known as an Arp2/3 complex activator in processes like endocytosis, extracellular matrix degradation, and host-pathogen interaction. In this issue of Developmental Cell, Juin et al. establish a novel trafficking function for N-WASP in driving lysophosphatidic acid-dependent c...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2019.10.029
更新日期:2019-11-18 00:00:00
abstract::Small GTPase Cdc42 triggers polarity establishment in budding yeast. In this issue of Developmental Cell, Okada et al. (2013) combine in silico modeling and cell biology to show that Cdc42, septins, and the exocytosis pathway are integrated in a feedback system to define and insulate the site of polarity in the membra...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2013.07.004
更新日期:2013-07-29 00:00:00
abstract::The insulin-like peptide (ILP) family plays key biological roles in the control of body growth. Although the functions of ILPs are well understood, the mechanisms by which organisms sense their nutrient status and thereby control ILP production remain largely unknown. Here, we show that signaling relay and feedback me...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.10.003
更新日期:2015-11-09 00:00:00
abstract::How cells sense their physical microenvironment remains incompletely understood. In two recent Science articles, Lomakin et al. (2020) and Venturini et al. (2020) demonstrate that progressive nuclear deformation associated with cellular confinement triggers intracellular events that promote cell contractility and migr...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2021.01.002
更新日期:2021-01-25 00:00:00
abstract::TC10, a Rho family GTPase, has been shown to play an important role in the exocytosis of GLUT4 and other proteins, primarily by tethering the vesicles at the plasma membrane. Using a newly developed probe based on fluorescence resonance energy transfer, we found that TC10 activity at tethered vesicles dropped immediat...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2006.07.008
更新日期:2006-09-01 00:00:00
abstract::SnoN regulates multiple signaling pathways, including TGF-β/Smad and p53, and displays both pro-oncogenic and anti-oncogenic activities in human cancer. We have observed previously that both its intracellular localization and expression levels are sensitive to cell density, suggesting that it may crosstalk with Hippo ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.05.002
更新日期:2016-06-06 00:00:00