Abstract:
:The pathogenicity of Mycobacterium ulcerans (Buruli ulcer) is closely associated with the secretion of exotoxin mycolactone. The cytotoxicity of mycolactone has been linked to its apoptogenic activity. We explored if low mycolactone concentrations, which are not able to induce apoptosis, can influence other essential activities on two primary human keratinocyte populations, keratinocyte stem cells (KSC) and transit amplifying cells (TAC), and on a human keratinocyte line, HaCaT. We demonstrated that 0.01 and 0.1 ng/ml mycolactone A/B are not able to induce apoptosis in primary human keratinocytes, but interfere with KSC wound repair. Moreover, the same toxin concentrations reduce cell proliferation of KSC and TAC and their ability to adhere to type IV collagen. HaCaT cells are more resistant to the toxin; nevertheless, they show a delayed woud repair when treated with 1 and 10 ng/ml mycolactone A/B. Moreover, these sub-apoptotic concentrations affect their ability to proliferate and adhere to collagen IV. Wound healing is a complex mechanism, which occurs "in vivo" as the outcome of many co-ordinated events. Sub-apoptotic mycolactone concentrations can affect essential mechanisms, which are required to achieve wound repair, such as adhesion, migration and proliferation of human keratinocytes.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Graziola F,Colombo E,Tiberio R,Leigheb G,Bozzo Cdoi
10.1007/s00403-017-1719-2subject
Has Abstractpub_date
2017-04-01 00:00:00pages
179-189issue
3eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-017-1719-2journal_volume
309pub_type
杂志文章abstract::The adenylate cyclase system of an established human trichilemmoma cell line was investigated. Stimulators of human epidermal adenylate cyclase system, epinephrine, histamine, adenosine, and prostaglandin E increased cyclic AMP levels of the trichilemmoma cells. The effects of epinephrine, histamine, and adenosine wer...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00413285
更新日期:1987-01-01 00:00:00
abstract::Currently, there are no accurate and simple methods available to measure this risk of atrophy in patients treated with topical glucocorticosteroids. In the present clinical trial, we validated a new score (Dermaphot(®) score) to assess the atrophogenic potential of glucocorticosteroids. 36 healthy adult volunteers wer...
journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/s00403-012-1297-2
更新日期:2013-04-01 00:00:00
abstract::Epstein-Barr virus (EBV) can infect epithelial cells as well as B lymphocytes. Infection of the male and female genital tracts has recently been demonstrated, and it has been suggested that the virus may be sexually transmissible. In our study we investigated whether EBV can be found in the anal region of sexually act...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00374084
更新日期:1995-01-01 00:00:00
abstract::Recent evidence indicates that the biologically active metabolite of vitamin D3, 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], has an effect on the regulation of the immune response. We investigated whether topical treatment of mice with 1 alpha,25(OH)2D3 influences the contact hypersensitivity (CHS) response to...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412982
更新日期:1989-01-01 00:00:00
abstract::No previous studies have convincingly linked sweating disturbance with the subsequent development of lichen planus (LP). Therefore, we investigated whether sweating disturbance could be specifically detected in LP lesions and how it could trigger lesion development. We utilized the impression mold technique (IMT), whi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-018-1882-0
更新日期:2019-01-01 00:00:00
abstract::Using a sensitive and modified solid-phase radioimmunoassay for detecting anti-cardiolipin antibodies, sera of 45 patients with lepromatous leprosy were examined. Nine of the 45 (20%) showed positive levels of anti-cardiolipin antibodies. Inhibition tests revealed that these antibodies significantly cross-reacted with...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00407745
更新日期:1986-01-01 00:00:00
abstract::Primary hyperhidrosis is a condition characterized by excessive sweating. The estimated prevalence is between 0.6 and 4.4%, and it can have economic, psychological, and social consequences for affected individuals. Family and genetic studies have suggested a genetic component in the inheritance of the disease. In this...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-019-01966-1
更新日期:2019-12-01 00:00:00
abstract::The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050308
更新日期:1998-06-01 00:00:00
abstract::The history of 188 caucasian patients with atopic dermatitis (AD) and of 2,151 family members has been analyzed. Of the AD patients 48% suffered from respiratory atopy (36% rhinitis, 28% asthma, and 15% both). AD showed by far the earliest onset of all atopic diseases: 50% of our patients had skin lesions before the a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00493466
更新日期:1990-01-01 00:00:00
abstract::Antimicrobial peptides and proteins (AMPs) play important roles in skin immune defense due to their capacity to inhibit growth of microbes. During intrauterine life, the skin immune system has to acquire the prerequisites to protect the newborn from infection in the hostile environment after birth, which includes the ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1340-y
更新日期:2013-08-01 00:00:00
abstract::Androgenetic alopecia (AGA) is a dermatological disorder of scalp hair loss characterized by a progressive miniaturization of hair follicles with shortened anagen phase leading to a decreased number of hairs on the scalp. It is a complex polygenic trait prevailing around two-thirds of the male population. Elevated exp...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-018-1826-8
更新日期:2018-07-01 00:00:00
abstract::The demonstration of cytokine mRNA expression in epidermal cells by the polymerase chain reaction technique preceded by reverse transcription (RT-PCR) requires linear test conditions (i.e. that the product obtained after amplification reflects the relative amounts of starting material) and high reproducibility, specif...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00370722
更新日期:1994-01-01 00:00:00
abstract::Interleukin-6-like (IL-6-like) immunoreactivity was sought in inflamed and normal human skin using the same immunohistochemical technique as for detection of neuropeptides. Such immunoreactivity was found in dermal and in a few intraepidermal nerve-like fibres in biopsy specimens from inflamed skin from patients with ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF02505230
更新日期:1996-07-01 00:00:00
abstract::Linoleic acid is required for the formation and maintenance of the epidermal barrier, but most of the current in vitro keratinocyte culture systems are linoleic acid-deficient. The aim of the present study was to examine the efficiency of linoleic acid uptake in human keratinocyte cultures grown under submerged and ai...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050430
更新日期:1999-07-01 00:00:00
abstract::Medical innovation is crucial to advancing our field, and physicians have the potential to play a leading role due to their daily patient care experiences. The objective of this study was to evaluate the interest in, and barriers to participating in innovation. Two surveys were conducted; the first cross-sectional sur...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-020-02118-6
更新日期:2020-08-09 00:00:00
abstract::Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF01105793
更新日期:1995-01-01 00:00:00
abstract::This study concerns 57 patients who fulfilled histological criteria for the diagnosis allergic vasculitis. For 37 of these patients, biopsy specimen were available from lesional and adjacent non-lesional skin. Histological signs of vasculitis were found at both sites, but in clinically normal skin the perivascular inf...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00406538
更新日期:1980-01-01 00:00:00
abstract::Solar radiation in the UVB range is absorbed primarily by the epidermal DNA where characteristic photodamage results in altered immune responses and mutagenic lesions. UVB exposure of the skin results in a profound upregulation of the anti-inflammatory cytokine, IL-10 and suppression of contact hypersensitivity (CHS)....
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0634-0
更新日期:2006-03-01 00:00:00
abstract::The roles of androgen hypersecretion, in situ enzyme activity, and androgen receptors in androgenetic alopecia in women are still a matter of debate. We studied 187 women with alopecia, which we graded I, II, or III, according to Ludwig's classification, and 21 healthy control women. All participants were subjected to...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030000184
更新日期:2000-12-01 00:00:00
abstract::Psoriasis was initially considered to represent a disease of abnormal epidermal keratinocyte proliferation. Proliferation of keratinocytes is restricted by apoptotic cell death to maintain a constant thickness of epidermis. Nucleosomes are mainly released by apoptotic cells. Tumor necrosis factor-α (TNF-α) is an impor...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1347-4
更新日期:2013-08-01 00:00:00
abstract::The expression of IL-8 in psoriasis has been clearly shown with the use of immunocytochemical, RT-PCR and in situ hybridization methods. The presence of its ligand, the IL-8 receptor, has been demonstrated by the RT-PCR technique. We report here a study of the expression of both IL-8 type A and B receptors by immunohi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050218
更新日期:1997-07-01 00:00:00
abstract::The study aimed at detecting differentially expressed proteins in the stratum corneum of dandruff versus non-dandruff scalps to better understand dandruff aetiology. iTRAQ-based quantitative proteomic analysis revealed a total of 68 differentially expressed biomarkers. A detailed analysis of their known physiological ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-016-1681-4
更新日期:2016-11-01 00:00:00
abstract::In the study described here we investigated the possibility of an association between the aggressiveness of melanoma and multidrug resistance phenotype by analyzing the expression and activity of P-glycoprotein (Pgp) in two genetically related transplantable hamster melanomas--a melanotic (Ma) and an amelanotic (Ab) f...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030000146
更新日期:2000-07-01 00:00:00
abstract::One of the primary events in the pathogenesis of acne vulgaris is abnormal follicular keratinization. Since oral isotretinoin therapy reduces follicular hyperkeratinization in acne, our study has been designed to determine whether epidermal lipid composition of the epithelium of sebaceous follicles is affected by isot...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417712
更新日期:1988-01-01 00:00:00
abstract::Glycerol, widely used as humectant, is known to protect against irritants and to accelerate recovery of irritated skin. However, most studies were done with topical formulations (i.e. emulsions) containing glycerol in relatively high amounts, preventing drawing conclusions from direct effects. In this study, acute che...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-1021-z
更新日期:2010-08-01 00:00:00
abstract::We investigated the effects of 1-4% ointments of metronidazole and tinidazole (derivatives of nitroimidazole) on models of inflammatory dermatitis evoked by antigen, hapten and monoclonal anti-dinitrophenol (DNP) IgE antibody in mice. Metronidazole and tinidazole ointments (1) suppressed the late-phase reaction (LPR) ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-002-0381-4
更新日期:2003-03-01 00:00:00
abstract::Whereas the inflammatory infiltrates of malignant melanoma have been widely investigated, little is known about the infiltrates accompanying benign melanocytic naevi. Using monoclonal antibodies directed against HLA-DR antigens, the CD1 antigen, the transferrin receptor and functionally divergent macrophage subpopulat...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372703
更新日期:1992-01-01 00:00:00
abstract::The glycolytic enzyme α-enolase is a plasminogen-binding protein that is generally found in the cytosolic compartment. However, α-enolase can also be expressed on cell surfaces following an inflammatory stimulus via an unknown mechanism. We investigated the effects of Streptococcus sanguinis (S. sanguinis) and the ser...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1298-1
更新日期:2013-04-01 00:00:00
abstract::Forty-one male guinea pigs were treated orally with high doses of aromatic retinoid (Ro 10-9359) 25 mg/kg/day in oleum arachidis for 6 weeks. Twenty-three animals were investigated at the end of this treatment. The remaining 18 animals were investigated after an additional retinoid-free interval of 6 weeks. A control ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00569101
更新日期:1980-01-01 00:00:00
abstract::Hidradenitis suppurativa (HS) is a chronic inflammatory disease associated with insulin resistance (IR), metabolic syndrome and increased cardiovascular risk. Adipokines are biologically active, pleotropic molecules which have been involved in the development of IR and in the pathogenesis of several chronic inflammato...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-019-02018-4
更新日期:2020-10-01 00:00:00