Inhibition of PDGFR signaling prevents muscular fatty infiltration after rotator cuff tear in mice.

Abstract:

:Fatty infiltration in muscle is often observed in patients with sizable rotator cuff tear (RCT) and is thought to be an irreversible event that significantly compromises muscle plasticity and contraction strength. These changes in the mechanical properties of the affected muscle render surgical repair of RCT highly formidable. Therefore, it is important to learn more about the pathology of fatty infiltration to prevent this undesired condition. In the present study, we aimed to generate a mouse model that can reliably recapitulate some of the important characteristics of muscular fatty infiltration after RCT in humans. We found that fatty infiltration can be efficiently induced by a combination of the following procedures: denervation of the suprascapular nerve, transection of the rotator cuff tendon, and resection of the humeral head. Using this model, we found that platelet-derived growth factor receptor-α (PDGFRα)-positive mesenchymal stem cells are induced after this intervention and that inhibition of PDGFR signaling by imatinib treatment can significantly suppress fatty infiltration. Taken together, the present study presents a reliable fatty infiltration mouse model and suggests a key role for PDGFRα-positive mesenchymal stem cells in the process of fatty infiltration after RCT in humans.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Shirasawa H,Matsumura N,Shimoda M,Oki S,Yoda M,Tohmonda T,Kanai Y,Matsumoto M,Nakamura M,Horiuchi K

doi

10.1038/srep41552

subject

Has Abstract

pub_date

2017-01-31 00:00:00

pages

41552

issn

2045-2322

pii

srep41552

journal_volume

7

pub_type

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