Abstract:
:During puberty, the mouse mammary gland develops into a highly branched epithelial network. Owing to the absence of exclusive stem cell markers, the location, multiplicity, dynamics and fate of mammary stem cells (MaSCs), which drive branching morphogenesis, are unknown. Here we show that morphogenesis is driven by proliferative terminal end buds that terminate or bifurcate with near equal probability, in a stochastic and time-invariant manner, leading to a heterogeneous epithelial network. We show that the majority of terminal end bud cells function as highly proliferative, lineage-committed MaSCs that are heterogeneous in their expression profile and short-term contribution to ductal extension. Yet, through cell rearrangements during terminal end bud bifurcation, each MaSC is able to contribute actively to long-term growth. Our study shows that the behaviour of MaSCs is not directly linked to a single expression profile. Instead, morphogenesis relies upon lineage-restricted heterogeneous MaSC populations that function as single equipotent pools in the long term.
journal_name
Naturejournal_title
Natureauthors
Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen Jdoi
10.1038/nature21046subject
Has Abstractpub_date
2017-02-16 00:00:00pages
313-317issue
7641eissn
0028-0836issn
1476-4687pii
nature21046journal_volume
542pub_type
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