Abstract:
:In vivo, bone morphogenetic protein 2 (BMP-2) exists both in solution and bound to the extracellular matrix (ECM). While these two modes of presentation are known to influence cell behavior distinctly, their role in the niche microenvironment and their functional relevance in the genesis of a biological response has sparsely been investigated at a cellular level. Here we used the natural affinity of BMP-2 for fibronectin (FN) to engineer cell-sized micropatterns of BMP-2. This technique allowed the simultaneous control of the spatial presentation of fibronectin-bound BMP-2 and cell spreading. These micropatterns induced a specific actin and adhesion organization around the nucleus, and triggered the phosphorylation and nuclear translocation of SMAD1/5/8 in C2C12 myoblasts and mesenchymal stem cells, an early indicator of their osteoblastic trans-differentiation. We found that cell spreading itself potentiated a BMP-2-dependent phosphorylation of SMAD1/5/8. Finally, we demonstrated that FN/BMP-2-mediated early SMAD signaling depended on LIM kinase 2 and ROCK, rather than myosin II activation. Altogether, our results show that FN/BMP-2 micropatterns are a useful tool to study the mechanisms underlying BMP-2-mediated mechanotransduction. More broadly, our approach could be adapted to other combinations of ECM proteins and growth factors, opening an exciting avenue to recreate tissue-specific niches in vitro.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Fitzpatrick V,Fourel L,Destaing O,Gilde F,Albigès-Rizo C,Picart C,Boudou Tdoi
10.1038/srep41479subject
Has Abstractpub_date
2017-01-30 00:00:00pages
41479issn
2045-2322pii
srep41479journal_volume
7pub_type
杂志文章abstract::Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and trans...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20758
更新日期:2016-02-11 00:00:00
abstract::The endocannabinoid 2-arachidonoylglycerol (2-AG) functions as a retrograde signaling molecule mediating synaptic transmission and plasticity at both inhibitory and excitatory synapses. However, little is known about whether 2-AG signaling is involved in homeostatic regulation of miniature synaptic events at excitator...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16257
更新日期:2015-11-06 00:00:00
abstract::A wide network of interconnected areas was previously found in neuroimaging studies involving normal as well as pathological subjects; however literature seems to suffer from a lack of investigation in glucose metabolism behaviour under olfactory condition. Thus, the present work describe for the first time a pure olf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05146
更新日期:2014-06-03 00:00:00
abstract::In this work, we analysed processive sliding and breakage of actin filaments at various heavy meromyosin (HMM) densities and ATP concentrations in IVMA. We observed that with addition of ATP solution, the actin filaments fragmented stochastically; we then determined mean length and velocity of surviving actin filament...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32043
更新日期:2016-08-24 00:00:00
abstract::We report on the fabrication of spherical Au spheres by pulsed laser treatment using a KrF excimer laser (248 nm, 25 ns) under ambient conditions as a fast and high throughput fabrication technique. The presented experiments were realized using initial Au layers of 100 nm thickness deposited on optically transparent a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29661-7
更新日期:2018-07-26 00:00:00
abstract::Mucociliary clearance is a biomechanical mechanism of airway protection. It consists of the active transport along the bronchial tree of the mucus, a fluid propelled by the coordinated beating of a myriad of cilia on the epithelial surface of the respiratory tract. The physics of mucus transport is poorly understood b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20882-4
更新日期:2018-02-05 00:00:00
abstract::In photodynamic therapy (PDT), cells are impregnated with a photosensitizing agent that is activated by light irradiation, thereby photochemically generating reactive oxygen species (ROS). The amounts of ROS produced depends on the PDT dose and the nature of the photosensitizer. Although high levels of ROS are cytotox...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13114
更新日期:2015-08-17 00:00:00
abstract::When estimating national PM2.5 concentrations, the results of traditional interpolation algorithms are unreliable due to a lack of monitoring sites and heterogeneous spatial distributions. PM2.5 spatial distribution is strongly correlated to elevation, and the information diffusion algorithm has been shown to be highl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14197-z
更新日期:2017-11-14 00:00:00
abstract::Pentameric ligand-gated ion channels (pLGICs) are targets of general anesthetics, but molecular mechanisms underlying anesthetic action remain debatable. We found that ELIC, a pLGIC from Erwinia chrysanthemi, can be functionally inhibited by isoflurane and other anesthetics. Structures of ELIC co-crystallized with iso...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13833
更新日期:2015-09-08 00:00:00
abstract::Ferroelectric tunneling junctions (FTJs) with tunable tunneling electroresistance (TER) are promising for many emerging applications, including non-volatile memories and neurosynaptic computing. One of the key challenges in FTJs is the balance between the polarization value and the tunneling current. In order to achie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56816-x
更新日期:2019-12-31 00:00:00
abstract::Textile forms of solar cells possess special advantages over other types of solar cells, including their light weight, high flexibility, and mechanical robustness. Recent demand for wearable devices has promoted interest in the development of high-efficiency textile-based solar cells for energy suppliers. However, the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05322
更新日期:2014-06-24 00:00:00
abstract::This work demonstrates that the rf-sputtering technique, combined with appropriate heat treatments, is potentially effective to develop new materials and devices based on oxide-interface and strain engineering. We report a study of the structural-physical properties relationship of high crystalline quality, highly ori...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17767-3
更新日期:2017-12-18 00:00:00
abstract::R.PabI is a type II restriction enzyme that recognizes the 5'-GTAC-3' sequence and belongs to the HALFPIPE superfamily. Although most restriction enzymes cleave phosphodiester bonds at specific sites by hydrolysis, R.PabI flips the guanine and adenine bases of the recognition sequence out of the DNA helix and hydrolyz...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35197
更新日期:2016-10-12 00:00:00
abstract::Predation has driven the evolution of diverse adaptations for defence among prey, and one striking example is the deimatic display. While such displays can resemble, or indeed co-occur with, aposematic 'warning' signals, theory suggests deimatic displays may function independently of predator learning. The survival va...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36995-9
更新日期:2019-01-24 00:00:00
abstract:BACKGROUND:Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency leads to impaired cortisol biosynthesis. Treatment includes glucocorticoid supplementation. We studied the specific metabolomics signatures in CAH patients using two different algorithms. METHODS:In a case-control study of CAH patients ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65897-y
更新日期:2020-06-01 00:00:00
abstract::Chronic consumption of excess ethanol increases the risk of colorectal cancer. The pathogenesis of ethanol-related colorectal cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bacteria in the colon and rectum convert ethanol to acetaldehyde (AcH), which is carcinogenic. However, the effec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27923
更新日期:2016-06-13 00:00:00
abstract::A Disintegrin and Metalloproteinase-15 (ADAM15) is a transmembrane protein involved in protein ectodomain shedding, cell adhesion and signalling. We previously cloned and characterised alternatively spliced variants of ADAM15 that differ in their intracellular domains and demonstrated correlation of the expression of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49021-3
更新日期:2019-08-29 00:00:00
abstract::The sulfur-selenium doped carbon quantum dots (S,Se-CQDs) were synthesized by one-step through hydrothermal method in this study, which have high fluorescence quantum yield (43%) and advanced ability to scavenge reactive oxygen species (ROS). They were characterized by transmission electron microscope (TEM), nuclear m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55996-w
更新日期:2019-12-23 00:00:00
abstract::Insulin resistance is associated with aging in mice and humans. We have previously shown that administration of recombinant GDF11 (rGDF11) to aged mice alters aging phenotypes in the brain, skeletal muscle, and heart. While the closely related protein GDF8 has a role in metabolism, limited data are available on the po...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61443-y
更新日期:2020-03-12 00:00:00
abstract::Upwelling off Vietnam in the South China Sea (SCS) and the Sumatra-Java upwelling in the Indian Ocean significantly modulate regional variation in climate. Although located in different hemispheres, these upwellings nearly concur during the boreal summer; both are the result of wind-induced Ekman divergence. Beyond se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40246-w
更新日期:2019-03-06 00:00:00
abstract::Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological and behavioral traits. Motor traits, however, have not been explored in zebrafish strains. We here applied a treadmill for fish (swimmill) an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52592-w
更新日期:2019-11-08 00:00:00
abstract::An early bottleneck in the rapid isolation of new antibody fragment binders using in vitro library approaches is the inertia encountered in acquiring and preparing soluble antigen fragments. In this report, we describe a simple, yet powerful strategy that exploits the properties of the SpyCatcher/SpyTag (SpyC/SpyT) co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49233-7
更新日期:2019-09-06 00:00:00
abstract::The increase in antibiotic resistant bacteria has raised global concern regarding the future effectiveness of antibiotics. Human activities that influence microbial communities and environmental resistomes can generate additional risks to human health. In this work, we characterized aquatic microbial communities and t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48200-6
更新日期:2019-08-13 00:00:00
abstract::Impurities in superconductors and their induced bound states are important both for engineering novel states such as Majorana zero-energy modes and for probing bulk properties of the superconducting state. The high-temperature cuprates offer a clear advantage in a much larger superconducting order parameter, but the n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44107
更新日期:2017-03-10 00:00:00
abstract::Quantum walks often provide telling insights about the structure of the system on which they are performed. In [Formula: see text]-symmetric and lossy dimer lattices, the topological properties of the band structure manifest themselves in the quantization of the mean displacement of such a walker. We investigate the f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30344-6
更新日期:2018-08-13 00:00:00
abstract::Succeeding in breeding super hybrid rice has been considered as a great progress in rice production in China. This on-farm study was conducted with Minirhizotron techniques to identify dynamic root morphological traits and distribution (0-30 cm) under different nitrogen treatments. Five elite super hybrid rice cultiva...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18576-4
更新日期:2018-01-09 00:00:00
abstract::Current approaches to predicting a cardiovascular disease (CVD) event rely on conventional risk factors and cross-sectional data. In this study, we applied machine learning and deep learning models to 10-year CVD event prediction by using longitudinal electronic health record (EHR) and genetic data. Our study cohort i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36745-x
更新日期:2019-01-24 00:00:00
abstract::In arid and semiarid regions, low precipitation rates lead to soil salinity problems, which may limit plant establishment, growth, and survival. Herein, we investigated the NaCl stress effect on chlorophyll fluorescence, photosynthetic-pigments, movement and chloroplasts ultrastructure in chlorenchyma cells of Opuntia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08744-x
更新日期:2017-08-17 00:00:00
abstract::Sirtuins are NAD(+) dependent lysine deacylases involved in many regulatory processes such as control of metabolic pathways, DNA repair and stress response. Modulators of sirtuin activity are required as tools for uncovering the biological function of these enzymes and as potential therapeutic agents. Systematic disco...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22643
更新日期:2016-03-04 00:00:00
abstract::To determine the effects of the estrogen-related receptor γ (ESRRG) rs1890552 A > G polymorphism on dietary advice-mediated changes in fasting glucose and arterial stiffness, 374 subjects with normal fasting glucose (NFG; control group, no treatment) and 142 subjects with impaired fasting glucose (IFG group, dietary a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10192-6
更新日期:2017-08-29 00:00:00