Abstract：
:Natural Bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. However, overproduction of surfactin in Bacillus amyloliquefaciens MT45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. To systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of B. amyloliquefaciens type strain DSM7T. MT45 possesses a smaller genome while containing more unique transporters and resistance-associated genes. Comparative transcriptome analysis revealed notable enrichment of the surfactin synthesis pathway in MT45, including central carbon metabolism and fatty acid biosynthesis to provide sufficient quantities of building precursors. Most importantly, the modular surfactin synthase overexpressed (9 to 49-fold) in MT45 compared to DSM7T suggested efficient surfactin assembly and resulted in the overproduction of surfactin. Furthermore, based on the expression trends observed in the transcriptome, there are multiple potential regulatory genes mediating the expression of surfactin synthase. Thus, the results of the present study provide new insights regarding the synthesis and regulation of surfactin in high-producing strain and enrich the genomic and transcriptomic resources available for B. amyloliquefaciens.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhi Y,Wu Q,Xu Ydoi
10.1038/srep40976subject
Has Abstractpub_date
2017-01-23 00:00:00pages
40976issn
2045-2322pii
srep40976journal_volume
7pub_type
杂志文章abstract：:Thermoelectric power production in the United States primarily relies on wet-cooled plants, which in turn require water below prescribed design temperatures, both for cooling and operational efficiency. Thus, power production in US remains particularly vulnerable to water scarcity and rising stream temperatures under ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12133-9
更新日期：2017-09-20 00:00:00
abstract：:Maculinea (=Phengaris) are endangered butterflies that are characterized by a very complex biological cycle. Maculinea larvae behave as obligate parasites whose survival is strictly dependent on both particular food plants and species-specific Myrmica ants. In this interaction, Maculinea caterpillars induce Myrmica wo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44514-7
更新日期：2019-05-29 00:00:00
abstract：:microRNAs regulate the expression of over 60% of protein coding genes by targeting their mRNAs to AGO2-containing complexes in the cytoplasm and promoting their translational inhibition and/or degradation. There is little evidence so far for microRNA-mediated regulation of other classes of non-coding RNAs. Here we rep...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02535
更新日期：2013-01-01 00:00:00
abstract：:Online gambling sites offer many different gambling games. In this work we analyse the gambling logs of numerous solely probability-based gambling games and extract the wager and odds distributions. We find that the log-normal distribution describes the wager distribution at the aggregate level. Viewing the gamblers' ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50168-2
更新日期：2019-10-11 00:00:00
abstract：:Scrophularia striata, commonly known as figwort, is one of the most important medicinal plants that mainly grows in cold regions of the Zagros Mountains (West of Iran). Although the chemical composition of this plant species has not yet been explored, people living in Ilam province (W Iran) have used it for many years...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52605-8
更新日期：2019-11-05 00:00:00
abstract：:This study assessed the efficacy and tolerability of intravenous ibuprofen in the improvement of post-operative pain control and the reduction of opioid usage. Patients were randomly divided into placebo, ibuprofen 400 mg and ibuprofen 800 mg groups. All patients received patient-controlled intravenous morphine analge...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-018-28428-4
更新日期：2018-07-05 00:00:00
abstract：:The placenta is an essential organ that is formed during pregnancy and its proper development is critical for embryonic survival. While several animal models have been shown to exhibit some of the pathological effects present in human preeclampsia, these models often do not represent the physiological aspects that hav...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39426-5
更新日期：2019-02-26 00:00:00
abstract：:Parasites and pathogens of the honey bee (Apis mellifera) are key factors underlying colony losses, which are threatening the beekeeping industry and agriculture as a whole. To control the spread and development of pathogen infections within the colony, honey bees use plant resins with antibiotic activity, but little ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06488-2
更新日期：2017-07-24 00:00:00
abstract：:Electroencephalographic hyperscanning was used to investigate interbrain synchronization patterns in dyads of participants interacting through speech. Results show that brain oscillations are synchronized between listener and speaker during oral narratives. This interpersonal synchronization is mediated in part by a l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04464-4
更新日期：2017-06-23 00:00:00
abstract：:This study aimed to investigate whether interleukin-34 (IL-34) mRNA expression is aberrant and modulated by tumor necrosis factor-alpha (TNF-α) in knee osteoarthritis (OA) fibroblast-like synoviocytes (FLS) and determine associations of IL-34 mRNA and protein in the systemic and local joint environments with severity ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64932-2
更新日期：2020-05-14 00:00:00
abstract：:Hair loss is a common medical problem. In this study, we investigated the proliferation, migration, and growth factor expression of human dermal papilla (DP) cells in the presence or absence of treatment with mesenchymal stem cell extracellular vesicles (MSC-EVs). In addition, we tested the efficacy of MSC-EV treatmen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15505-3
更新日期：2017-11-14 00:00:00
abstract：:We identify graphene layer on a disordered substrate as a system where localization of phonons can be observed. Generally, observation of localization for scattering waves is not simple, because the Rayleigh scattering is inversely proportional to a high power of wavelength. The situation is radically different for th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34426-3
更新日期：2018-11-02 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have various functions, making a significant contribution to tissue repair. On the other hand, the viability and function of MSCs are not lasting after an in vivo transplant, and the therapeutic effects of MSCs are limited. Although various chemical modification methods have been applied ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17166-8
更新日期：2017-12-05 00:00:00
abstract：:When humans fail to make optimal decisions in strategic games and economic gambles, researchers typically try to explain why that behaviour is biased. To this end, they search for mechanisms that cause human behaviour to deviate from what seems to be the rational optimum. But perhaps human behaviour is not biased; per...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34102
更新日期：2016-09-28 00:00:00
abstract：:Circadian disruption resulting from exposure to irregular light-dark patterns and sleep deprivation has been associated with beta amyloid peptide (Aβ) aggregation, which is a major event in Alzheimer's disease (AD) pathology. We exposed 5XFAD mice and littermate controls to dim-light vs. bright-light photophases to in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68199-5
更新日期：2020-07-10 00:00:00
abstract：:This study measured the effects of vitamin D (VD) supplementation on the underlying molecular pathways involved in renal and testicular damage induced by lead (Pb) toxicity. Thirty two adult male Wistar rats were divided equally into four groups that were treated individually or simultaneously, except the negative con...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23258-w
更新日期：2018-03-19 00:00:00
abstract：:The relationship between the cuprate pseudogap (Δ(p)) and superconducting gap (Δ(s)) remains an unsolved mystery. Here, we present a temperature- and doping-dependent tunneling study of submicron Bi(2)Sr(2)CaCu(2)O(8+δ) intrinsic Josephson junctions, which provides a clear evidence that Δ(s) closes at a temperature T(...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00248
更新日期：2012-01-01 00:00:00
abstract：:The gene PFKFB3 encodes for inducible 6-phosphofructo-2-kinase, a glycolysis-regulatory enzyme that protects against diet-induced intestine inflammation. However, it is unclear how nutrient overload regulates PFKFB3 expression and inflammatory responses in intestinal epithelial cells (IECs). In the present study, prim...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28963
更新日期：2016-07-08 00:00:00
abstract：:A bubble merged from two parent bubbles with different size tends to be placed closer to the larger parent. This phenomenon is known as the coalescence preference. Here we demonstrate that the coalescence preference can be blocked inside a densely packed cluster of bubbles. We utilized high-speed high-resolution X-ray...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07739
更新日期：2015-01-13 00:00:00
abstract：:The increasing antibiotic resistance evolved by Helicobacter pylori has alarmingly reduced the eradication rates of first-line therapies. To overcome the current circulating resistome, we selected a novel potential therapeutic target in order to identify new candidate drugs for treating H. pylori infection. We screene...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47746-9
更新日期：2019-08-05 00:00:00
abstract：:Diet composition impacts metabolic and cardiovascular health with high caloric diets contributing to obesity related disorders. Dietary interventions such as caloric restriction exert beneficial effects in the cardiovascular system, but alteration of which specific nutrient is responsible is less clear. This study inv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51654-3
更新日期：2019-10-22 00:00:00
abstract：:Pioneering epidemiological work has established strong association of sedentary lifestyle and obesity with the risk of colorectal cancer, while the detailed underlying mechanism remains unknown. Here we show that Hotair (HOX transcript antisense RNA) is a pro-adipogenic long non-coding RNA highly expressed in gluteal-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45648
更新日期：2017-03-31 00:00:00
abstract：:Polymer-derived silicon oxycarbide (SiCO) presents excellent performance for high temperature and lithium-ion battery applications. Current experiments have provided some information on nano-structure of SiCO, while it is very challenging for experiments to take further insight into the molecular structure and its rel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42705
更新日期：2017-02-14 00:00:00
abstract：:The binding of metal ions at the interface of protein complexes presents a unique and poorly understood mechanism of molecular assembly. A remarkable example is the Rad50 zinc hook domain, which is highly conserved and facilitates the Zn2+-mediated homodimerization of Rad50 proteins. Here, we present a detailed analys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36346
更新日期：2016-11-03 00:00:00
abstract：:Poor oral health is not only associated with diabetes and cardiovascular disease but adverse pregnancy outcomes. However the influence of dental caries on pregnancy is unknown. The aim of this study was to evaluate the association between dental caries and adverse pregnancy outcomes and the effect of treatment for den...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62306-2
更新日期：2020-03-24 00:00:00
abstract：:The endo-levanase BT1760 of a human gut commensal Bacteroides thetaiotaomicron randomly cuts a β-2,6-linked fructan, levan, into fructo-oligosaccharides providing a prebiotic substrate for gut microbiota. Here we introduce the crystal structure of BT1760 at resolution of 1.65 Å. The fold of the enzyme is typical for G...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44785-0
更新日期：2019-06-11 00:00:00
abstract：:We report here the design and multiple functions of a new hierarchical nanotheronostic platform consisting of an upconversion nanoparticle (UCNP) core: shell with an additional mesoporous silica (mSiO2) matrix load shell containing sealed, high concentration of ICG molecules. We demonstrate that this UCNP@mSiO2-ICG na...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16016-x
更新日期：2017-11-16 00:00:00
abstract：:We summarized published data on the associations of apolipoprotein E (APOE) gene ε2/ε3/ε4 polymorphism with both cancer risk and circulating lipid profiles, aiming to examine the causal relevance between lipids and cancer risk. Article identification and data abstraction were conducted in duplicate and independently b...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep09495
更新日期：2015-03-30 00:00:00
abstract：:Using dedicated contrast agents high-quality X-ray imaging of soft tissue structures with isotropic micrometre resolution has become feasible. This technique is frequently titled as virtual histology as it allows production of slices of tissue without destroying the sample. The use of contrast agents is, however, ofte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30385
更新日期：2016-07-27 00:00:00
abstract：:The rapid increase in use of electronic-cigarettes (e-cigarettes), especially among youth, raises the urgency for regulating bodies to make informed decisions, guidance, and policy on these products. This study evaluated cardiac function in an experimental model following exposure to e-cigarettes. We subjected C57BL/6...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40847-5
更新日期：2019-03-11 00:00:00
