The unique genomic landscape surrounding the EPSPS gene in glyphosate resistant Amaranthus palmeri: a repetitive path to resistance.

Abstract:

BACKGROUND:The expanding number and global distributions of herbicide resistant weedy species threaten food, fuel, fiber and bioproduct sustainability and agroecosystem longevity. Amongst the most competitive weeds, Amaranthus palmeri S. Wats has rapidly evolved resistance to glyphosate primarily through massive amplification and insertion of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene across the genome. Increased EPSPS gene copy numbers results in higher titers of the EPSPS enzyme, the target of glyphosate, and confers resistance to glyphosate treatment. To understand the genomic unit and mechanism of EPSPS gene copy number proliferation, we developed and used a bacterial artificial chromosome (BAC) library from a highly resistant biotype to sequence the local genomic landscape flanking the EPSPS gene. RESULTS:By sequencing overlapping BACs, a 297 kb sequence was generated, hereafter referred to as the "EPSPS cassette." This region included several putative genes, dense clusters of tandem and inverted repeats, putative helitron and autonomous replication sequences, and regulatory elements. Whole genome shotgun sequencing (WGS) of two biotypes exhibiting high and no resistance to glyphosate was performed to compare genomic representation across the EPSPS cassette. Mapping of sequences for both biotypes to the reference EPSPS cassette revealed significant differences in upstream and downstream sequences relative to EPSPS with regard to both repetitive units and coding content between these biotypes. The differences in sequence may have resulted from a compounded-building mechanism such as repetitive transpositional events. The association of putative helitron sequences with the cassette suggests a possible amplification and distribution mechanism. Flow cytometry revealed that the EPSPS cassette added measurable genomic content. CONCLUSIONS:The adoption of glyphosate resistant cropping systems in major crops such as corn, soybean, cotton and canola coupled with excessive use of glyphosate herbicide has led to evolved glyphosate resistance in several important weeds. In Amaranthus palmeri, the amplification of the EPSPS cassette, characterized by a complex array of repetitive elements and putative helitron sequences, suggests an adaptive structural genomic mechanism that drives amplification and distribution around the genome. The added genomic content not found in glyphosate sensitive plants may be driving evolution through genome expansion.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Molin WT,Wright AA,Lawton-Rauh A,Saski CA

doi

10.1186/s12864-016-3336-4

subject

Has Abstract

pub_date

2017-01-17 00:00:00

pages

91

issue

1

issn

1471-2164

pii

10.1186/s12864-016-3336-4

journal_volume

18

pub_type

杂志文章
  • Monophyly of clade III nematodes is not supported by phylogenetic analysis of complete mitochondrial genome sequences.

    abstract:BACKGROUND:The orders Ascaridida, Oxyurida, and Spirurida represent major components of zooparasitic nematode diversity, including many species of veterinary and medical importance. Phylum-wide nematode phylogenetic hypotheses have mainly been based on nuclear rDNA sequences, but more recently complete mitochondrial (m...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-392

    authors: Park JK,Sultana T,Lee SH,Kang S,Kim HK,Min GS,Eom KS,Nadler SA

    更新日期:2011-08-03 00:00:00

  • Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens.

    abstract:BACKGROUND:Lower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell formation is a highly complex process under precise regulation of genes and bio...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07177-7

    authors: Feng J,Zhang HJ,Wu SG,Qi GH,Wang J

    更新日期:2020-11-09 00:00:00

  • RNA profiles of rat olfactory epithelia: individual and age related variations.

    abstract:BACKGROUND:Mammalian genomes contain a large number (approximately 1000) of olfactory receptor (OR) genes, many of which (20 to 50%) are pseudogenes. OR gene transcription is not restricted to the olfactory epithelium, but is found in numerous tissues. Using microarray hybridization and RTqPCR, we analyzed the mRNA pro...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-572

    authors: Rimbault M,Robin S,Vaysse A,Galibert F

    更新日期:2009-12-02 00:00:00

  • Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis.

    abstract:BACKGROUND:Epidermal Growth Factor (EGF) is a key regulatory growth factor activating many processes relevant to normal development and disease, affecting cell proliferation and survival. Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-326

    authors: Llorens F,Hummel M,Pastor X,Ferrer A,Pluvinet R,Vivancos A,Castillo E,Iraola S,Mosquera AM,González E,Lozano J,Ingham M,Dohm JC,Noguera M,Kofler R,del Río JA,Bayés M,Himmelbauer H,Sumoy L

    更新日期:2011-06-23 00:00:00

  • Systematic sequencing of mRNA from the Antarctic krill (Euphausia superba) and first tissue specific transcriptional signature.

    abstract:BACKGROUND:Little is known about the genome sequences of Euphausiacea (krill) although these crustaceans are abundant components of the pelagic ecosystems in all oceans and used for aquaculture and pharmaceutical industry. This study reports the results of an expressed sequence tag (EST) sequencing project from differe...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-45

    authors: De Pittà C,Bertolucci C,Mazzotta GM,Bernante F,Rizzo G,De Nardi B,Pallavicini A,Lanfranchi G,Costa R

    更新日期:2008-01-28 00:00:00

  • Fast multiclonal clusterization of V(D)J recombinations from high-throughput sequencing.

    abstract:BACKGROUND:V(D)J recombinations in lymphocytes are essential for immunological diversity. They are also useful markers of pathologies. In leukemia, they are used to quantify the minimal residual disease during patient follow-up. However, the full breadth of lymphocyte diversity is not fully understood. RESULTS:We prop...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-409

    authors: Giraud M,Salson M,Duez M,Villenet C,Quief S,Caillault A,Grardel N,Roumier C,Preudhomme C,Figeac M

    更新日期:2014-05-28 00:00:00

  • The venom composition of the parasitic wasp Chelonus inanitus resolved by combined expressed sequence tags analysis and proteomic approach.

    abstract:BACKGROUND:Parasitic wasps constitute one of the largest group of venomous animals. Although some physiological effects of their venoms are well documented, relatively little is known at the molecular level on the protein composition of these secretions. To identify the majority of the venom proteins of the endoparasit...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-693

    authors: Vincent B,Kaeslin M,Roth T,Heller M,Poulain J,Cousserans F,Schaller J,Poirié M,Lanzrein B,Drezen JM,Moreau SJ

    更新日期:2010-12-07 00:00:00

  • Whole genome resequencing of the Iranian native dogs and wolves to unravel variome during dog domestication.

    abstract:BACKGROUND:Advances in genome technology have simplified a new comprehension of the genetic and historical processes crucial to rapid phenotypic evolution under domestication. To get new insight into the genetic basis of the dog domestication process, we conducted whole-genome sequence analysis of three wolves and thre...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6619-8

    authors: Amiri Ghanatsaman Z,Wang GD,Asadollahpour Nanaei H,Asadi Fozi M,Peng MS,Esmailizadeh A,Zhang YP

    更新日期:2020-03-04 00:00:00

  • Identification and evolutionary analysis of the nucleolar proteome of Giardia lamblia.

    abstract:BACKGROUND:The nucleoli, including their proteomes, of higher eukaryotes have been extensively studied, while few studies about the nucleoli of the lower eukaryotes - protists were reported. Giardia lamblia, a protist with the controversy of whether it is an extreme primitive eukaryote or just a highly evolved parasite...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-6679-9

    authors: Feng JM,Yang CL,Tian HF,Wang JX,Wen JF

    更新日期:2020-03-30 00:00:00

  • Five distinct biological processes and 14 differentially expressed genes characterize TEL/AML1-positive leukemia.

    abstract:BACKGROUND:The t(12;21)(p13;q22) translocation is found in 20 to 25% of cases of childhood B-lineage acute lymphoblastic leukemia (B-ALL). This rearrangement results in the fusion of ETV6 (TEL) and RUNX1 (AML1) genes and defines a relatively uniform category, although only some patients suffer very late relapse. TEL/AM...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-8-385

    authors: Gandemer V,Rio AG,de Tayrac M,Sibut V,Mottier S,Ly Sunnaram B,Henry C,Monnier A,Berthou C,Le Gall E,Le Treut A,Schmitt C,Le Gall JY,Mosser J,Galibert MD

    更新日期:2007-10-23 00:00:00

  • An advanced bioinformatics approach for analyzing RNA-seq data reveals sigma H-dependent regulation of competence genes in Listeria monocytogenes.

    abstract:BACKGROUND:Alternative σ factors are important transcriptional regulators in bacteria. While σ(B) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen Listeria monocytogenes, the function of σ(H) has not yet been well defined in Listeria, even though σ(H) c...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2432-9

    authors: Liu Y,Orsi RH,Boor KJ,Wiedmann M,Guariglia-Oropeza V

    更新日期:2016-02-16 00:00:00

  • Parallel selection on gene copy number variations through evolution of three-spined stickleback genomes.

    abstract:BACKGROUND:Understanding the genetic basis of adaptive evolution is one of the major goals in evolutionary biology. Recently, it has been revealed that gene copy number variations (GCNVs) constitute significant proportions of genomic diversities within natural populations. However, it has been unclear whether GCNVs are...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-735

    authors: Hirase S,Ozaki H,Iwasaki W

    更新日期:2014-08-29 00:00:00

  • The serine/threonine kinase 33 is present and expressed in palaeognath birds but has become a unitary pseudogene in neognaths about 100 million years ago.

    abstract:BACKGROUND:Serine/threonine kinase 33 (STK33) has been shown to be conserved across all major vertebrate classes including reptiles, mammals, amphibians and fish, suggesting its importance within vertebrates. It has been shown to phosphorylate vimentin and might play a role in spermatogenesis and organ ontogenesis. In ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-1769-9

    authors: Lautwein T,Lerch S,Schäfer D,Schmidt ER

    更新日期:2015-07-22 00:00:00

  • Comparative miRNAome analysis revealed different miRNA expression profiles in bovine sera and exosomes.

    abstract:BACKGROUND:Extra-cellular components, such as serum and exosome, have drawn great attention as a readily accessible source of biomarkers for mammalian health. However, the contribution of different blood components to the signature of respective microRNAs (miRNAs) remains unknown, especially in cattle. In this study we...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2962-1

    authors: Zhao K,Liang G,Sun X,Guan le L

    更新日期:2016-08-12 00:00:00

  • Computational approach to predict species-specific type III secretion system (T3SS) effectors using single and multiple genomes.

    abstract:BACKGROUND:Many gram-negative bacteria use type III secretion systems (T3SSs) to translocate effector proteins into host cells. T3SS effectors can give some bacteria a competitive edge over others within the same environment and can help bacteria to invade the host cells and allow them to multiply rapidly within the ho...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-3363-1

    authors: Hobbs CK,Porter VL,Stow ML,Siame BA,Tsang HH,Leung KY

    更新日期:2016-12-19 00:00:00

  • Transcriptome analysis of human brain tissue identifies reduced expression of complement complex C1Q Genes in Rett syndrome.

    abstract:BACKGROUND:MECP2, the gene mutated in the majority of Rett syndrome cases, is a transcriptional regulator that can activate or repress transcription. Although the transcription regulatory function of MECP2 has been known for over a decade, it remains unclear how transcriptional dysregulation leads to the neurodevelopme...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2746-7

    authors: Lin P,Nicholls L,Assareh H,Fang Z,Amos TG,Edwards RJ,Assareh AA,Voineagu I

    更新日期:2016-06-06 00:00:00

  • De novo transcriptome reconstruction and annotation of the Egyptian rousette bat.

    abstract:BACKGROUND:The Egyptian Rousette bat (Rousettus aegyptiacus), a common fruit bat species found throughout Africa and the Middle East, was recently identified as a natural reservoir host of Marburg virus. With Ebola virus, Marburg virus is a member of the family Filoviridae that causes severe hemorrhagic fever disease i...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-2124-x

    authors: Lee AK,Kulcsar KA,Elliott O,Khiabanian H,Nagle ER,Jones ME,Amman BR,Sanchez-Lockhart M,Towner JS,Palacios G,Rabadan R

    更新日期:2015-12-07 00:00:00

  • Differential gene expression and Hog1 interaction with osmoresponsive genes in the extremely halotolerant black yeast Hortaea werneckii.

    abstract:BACKGROUND:Fluctuations in external salinity force eukaryotic cells to respond by changes in the gene expression of proteins acting in protective biochemical processes, thus counteracting the changing osmotic pressure. The high-osmolarity glycerol (HOG) signaling pathway is essential for the efficient up-regulation of ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-8-280

    authors: Vaupotic T,Plemenitas A

    更新日期:2007-08-16 00:00:00

  • Systems genetics analysis of body weight and energy metabolism traits in Drosophila melanogaster.

    abstract:BACKGROUND:Obesity and phenotypic traits associated with this condition exhibit significant heritability in natural populations of most organisms. While a number of genes and genetic pathways have been implicated to play a role in obesity associated traits, the genetic architecture that underlies the natural variation ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-297

    authors: Jumbo-Lucioni P,Ayroles JF,Chambers MM,Jordan KW,Leips J,Mackay TF,De Luca M

    更新日期:2010-05-11 00:00:00

  • A UML profile for the OBO relation ontology.

    abstract:BACKGROUND:Ontologies have increasingly been used in the biomedical domain, which has prompted the emergence of different initiatives to facilitate their development and integration. The Open Biological and Biomedical Ontologies (OBO) Foundry consortium provides a repository of life-science ontologies, which are develo...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-S5-S3

    authors: Guardia GD,Vêncio RZ,de Farias CR

    更新日期:2012-01-01 00:00:00

  • Transcriptome analysis reveals differentially expressed genes associated with germ cell and gonad development in the Southern bluefin tuna (Thunnus maccoyii).

    abstract:BACKGROUND:Controlling and managing the breeding of bluefin tuna (Thunnus spp.) in captivity is an imperative step towards obtaining a sustainable supply of these fish in aquaculture production systems. Germ cell transplantation (GCT) is an innovative technology for the production of inter-species surrogates, by transp...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2397-8

    authors: Bar I,Cummins S,Elizur A

    更新日期:2016-03-10 00:00:00

  • Whole genome sequencing identified a 16 kilobase deletion on ECA13 associated with distichiasis in Friesian horses.

    abstract:BACKGROUND:Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of the Meibomian glands of the eyelid, has been reported in Friesian horses. These misplaced cilia can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to corneal fibrosis, ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07265-8

    authors: Hisey EA,Hermans H,Lounsberry ZT,Avila F,Grahn RA,Knickelbein KE,Duward-Akhurst SA,McCue ME,Kalbfleisch TS,Lassaline ME,Back W,Bellone RR

    更新日期:2020-11-30 00:00:00

  • A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants.

    abstract:BACKGROUND:Most published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongy...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4656-3

    authors: Pilkington SM,Crowhurst R,Hilario E,Nardozza S,Fraser L,Peng Y,Gunaseelan K,Simpson R,Tahir J,Deroles SC,Templeton K,Luo Z,Davy M,Cheng C,McNeilage M,Scaglione D,Liu Y,Zhang Q,Datson P,De Silva N,Gardiner SE,Bas

    更新日期:2018-04-16 00:00:00

  • Differentially expressed genes for aggressive pecking behaviour in laying hens.

    abstract:BACKGROUND:Aggressive behaviour is an important aspect in the daily lives of animals living in groups. Aggressive animals have advantages, such as better access to food or territories, and they produce more offspring than low ranking animals. The social hierarchy in chickens is measured using the 'pecking order' concep...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-544

    authors: Buitenhuis B,Hedegaard J,Janss L,Sørensen P

    更新日期:2009-11-19 00:00:00

  • Conditional entropy in variation-adjusted windows detects selection signatures associated with expression quantitative trait loci (eQTLs).

    abstract:BACKGROUND:Over the past 50,000 years, shifts in human-environmental or human-human interactions shaped genetic differences within and among human populations, including variants under positive selection. Shaped by environmental factors, such variants influence the genetics of modern health, disease, and treatment outc...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-16-S8-S8

    authors: Handelman SK,Seweryn M,Smith RM,Hartmann K,Wang D,Pietrzak M,Johnson AD,Kloczkowski A,Sadee W

    更新日期:2015-01-01 00:00:00

  • The host-pathogen interaction between wheat and yellow rust induces temporally coordinated waves of gene expression.

    abstract:BACKGROUND:Understanding how plants and pathogens modulate gene expression during the host-pathogen interaction is key to uncovering the molecular mechanisms that regulate disease progression. Recent advances in sequencing technologies have provided new opportunities to decode the complexity of such interactions. In th...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2684-4

    authors: Dobon A,Bunting DC,Cabrera-Quio LE,Uauy C,Saunders DG

    更新日期:2016-05-20 00:00:00

  • De novo sequencing and transcriptome analysis of the desert shrub, Ammopiptanthus mongolicus, during cold acclimation using Illumina/Solexa.

    abstract:BACKGROUND:Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f., an evergreen broadleaf legume shrub, is distributed in Mid-Asia where the temperature can be as low as -30°C during the winter. Although A. mongolicus is an ideal model to study the plant response to cold stress, insufficient genomic resources for this spe...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-14-488

    authors: Pang T,Ye CY,Xia X,Yin W

    更新日期:2013-07-18 00:00:00

  • Highly expressed captured genes and cross-kingdom domains present in Helitrons create novel diversity in Pleurotus ostreatus and other fungi.

    abstract:BACKGROUND:Helitrons are class-II eukaryotic transposons that transpose via a rolling circle mechanism. Due to their ability to capture and mobilize gene fragments, they play an important role in the evolution of their host genomes. We have used a bioinformatics approach for the identification of helitrons in two Pleur...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-15-1071

    authors: Castanera R,Pérez G,López L,Sancho R,Santoyo F,Alfaro M,Gabaldón T,Pisabarro AG,Oguiza JA,Ramírez L

    更新日期:2014-12-05 00:00:00

  • Sequence comparison of prefrontal cortical brain transcriptome from a tame and an aggressive silver fox (Vulpes vulpes).

    abstract:BACKGROUND:Two strains of the silver fox (Vulpes vulpes), with markedly different behavioral phenotypes, have been developed by long-term selection for behavior. Foxes from the tame strain exhibit friendly behavior towards humans, paralleling the sociability of canine puppies, whereas foxes from the aggressive strain a...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-482

    authors: Kukekova AV,Johnson JL,Teiling C,Li L,Oskina IN,Kharlamova AV,Gulevich RG,Padte R,Dubreuil MM,Vladimirova AV,Shepeleva DV,Shikhevich SG,Sun Q,Ponnala L,Temnykh SV,Trut LN,Acland GM

    更新日期:2011-10-03 00:00:00

  • The arrhythmogenic cardiomyopathy-specific coding and non-coding transcriptome in human cardiac stromal cells.

    abstract:BACKGROUND:Arrhythmogenic cardiomyopathy (ACM) is a genetic autosomal disease characterized by abnormal cell-cell adhesion, cardiomyocyte death, progressive fibro-adipose replacement of the myocardium, arrhythmias and sudden death. Several different cell types contribute to the pathogenesis of ACM, including, as recent...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4876-6

    authors: Rainer J,Meraviglia V,Blankenburg H,Piubelli C,Pramstaller PP,Paolin A,Cogliati E,Pompilio G,Sommariva E,Domingues FS,Rossini A

    更新日期:2018-06-25 00:00:00