Abstract:
:Long noncoding RNAs (lncRNAs) are important for gene expression, but little is known about their structures. RepA is a 1.6-kb mouse lncRNA comprising the same sequence as the 5' region of Xist, including A and F repeats. It has been proposed to facilitate the initiation and spread of X-chromosome inactivation, although its exact role is poorly understood. To gain insight into the molecular mechanism of RepA and Xist, we determined a complete phylogenetically validated secondary-structural map of RepA through SHAPE and DMS chemical probing of a homogeneously folded RNA in vitro. We combined UV-cross-linking experiments with RNA modeling methods to produce a three-dimensional model of RepA functional domains demonstrating that tertiary architecture exists within lncRNA molecules and occurs within specific functional modules. This work provides a foundation for understanding of the evolution and functional properties of RepA and Xist and offers a framework for exploring architectural features of other lncRNAs.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Liu F,Somarowthu S,Pyle AMdoi
10.1038/nchembio.2272subject
Has Abstractpub_date
2017-03-01 00:00:00pages
282-289issue
3eissn
1552-4450issn
1552-4469pii
nchembio.2272journal_volume
13pub_type
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