Abstract：
:Development of new antimicrobial agents is required against the causative agent for listeriosis, Listeria monocytogenes, as the number of drug resistant strains continues to increase. A promising target is the β-ketoacyl-acyl carrier protein synthase FabF, which participates in the catalysis of fatty acid synthesis and elongation, and is required for the production of phospholipid membranes, lipoproteins, and lipopolysaccharides. In this study, we report the 1.35 Å crystal structure of FabF from L. monocytogenes, providing an excellent platform for the rational design of novel inhibitors. By comparing the structure of L. monocytogenes FabF with other published bacterial FabF structures in complex with known inhibitors and substrates, we highlight conformational changes within the active site, which will need to be accounted for during drug design and virtual screening studies. This high-resolution structure of FabF represents an important step in the development of new classes of antimicrobial agents targeting FabF for the treatment of listeriosis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Soares da Costa TP,Nanson JD,Forwood JKdoi
10.1038/srep39277subject
Has Abstractpub_date
2017-01-03 00:00:00pages
39277issn
2045-2322pii
srep39277journal_volume
7pub_type
杂志文章abstract：:Particles manipulation with optical forces is known as optical tweezing. While tweezing in free space with laser beams was established in the 1980s, integrating the optical tweezers on a chip is a challenging task. Recent experiments with plasmonic nanoantennas, microring resonators, and photonic crystal nanocavities ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02290
更新日期：2013-01-01 00:00:00
abstract：:From mid-May to August 2011, extreme runoff in the Columbia River ranged from 14,000 to over 17,000 m(3)/s, more than two standard deviations above the mean for this period. The extreme runoff was the direct result of both melting of anomalously high snowpack and rainfall associated with the 2010-2011 La Niña. The eff...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17145
更新日期：2015-11-26 00:00:00
abstract：:Medium chain fatty acids can activate the pro-inflammatory receptor GPR84 but so also can molecules related to 3,3'-diindolylmethane. 3,3'-Diindolylmethane and decanoic acid acted as strong positive allosteric modulators of the function of each other and analysis showed the affinity of 3,3'-diindolylmethane to be at l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18159-3
更新日期：2017-12-20 00:00:00
abstract：:Tolerance to Dutch elm disease (DED) has been linked to the rapid and/or high induction of disease-responsive genes after infection with the fungus Ophiostoma novo-ulmi. Although the fungal infection by O. novo-ulmi primarily takes places in xylem vessels, it is still unclear how xylem contributes to the defense again...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07779-4
更新日期：2017-08-02 00:00:00
abstract：:Predictive coding theory suggests that predictable responses are "explained away" (i.e., reduced) by feedback. Experimental evidence for feedback inhibition, however, is inconsistent: most neuroimaging studies show reduced activity by predictive feedback, while neurophysiology indicates that most inter-areal cortical ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22944
更新日期：2016-03-14 00:00:00
abstract：:Target-guided synthesis is an approach to drug discovery that allows the target to self-assemble its own binding agents. So far, target-guided synthesis and especially in situ click chemistry have attracted extensive attention and have led to the identification of highly potent inhibitors for proteins. In this study, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17202
更新日期：2015-11-25 00:00:00
abstract：:Spectra-structure interrelationship is still the weakness of NIR spectral assignment. In this paper, a comprehensive investigation from chemical structural property to natural chemical compounds was carried out for NIR spectral assignment. Surprisingly, we discovered that NIR absorption frequency of the skeleton struc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45945-y
更新日期：2019-07-01 00:00:00
abstract：:We propose a scheme of designing polygonal rotator with homogenous materials by using linear coordinate transformation. Our strategy is available for both time-harmonic electromagnetic field case and static field case. In particular, we found that only one anisotropic material is needed in static field case, and the d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51637-4
更新日期：2019-10-22 00:00:00
abstract：:There is a growing interest concerning the relevance of salivary cortisone levels in stress-related research. However, studies investigating morning patterns and day-to-day variability of cortisone versus cortisol levels are lacking. Cortisol and cortisone analysis by liquid chromatography-tandem mass spectroscopy (LC...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55571-3
更新日期：2019-12-17 00:00:00
abstract：:Corticosteroids have been proved to be ineffective for Guillain-Barré syndrome, but the mechanism remains unknown. In a rabbit model of axonal Guillain-Barré syndrome, treatment with corticosteroids significantly reduced macrophage infiltration in the spinal ventral roots and the survival rate as well as clinical impr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13931
更新日期：2015-09-10 00:00:00
abstract：:Junctophilin-2 (JPH2) is a part of the junctional membrane complex that facilitates calcium-handling in the cardiomyocyte. Previously, missense variants in JPH2 have been linked to hypertrophic cardiomyopathy; however, pathogenic "loss of function" (LOF) variants have not been described. Family-based genetic analysis ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44987-6
更新日期：2019-06-21 00:00:00
abstract：:The lateral movement of soil carbon has a profound effect on the carbon budget of terrestrial ecosystems; however, it has never been quantified in China, which is one of the strongest soil erosion areas in the world. In this study, we estimated that the overall soil erosion in China varies from 11.27 to 18.17 Pg yr(-1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07247
更新日期：2014-11-28 00:00:00
abstract：:The occurrence of biochemical alterations that last for a long period of time in diabetic individuals even after adequate handling of glycemia is an intriguing phenomenon named metabolic memory. In this study, we show that a kidney pathway is gradually altered during the course of diabetes and remains persistently cha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40544
更新日期：2017-01-12 00:00:00
abstract：:Crystal and electronic structures of ~380 nm BiFeO3 film grown on LaAlO3 substrate are comprehensively studied using advanced transmission electron microscopy (TEM) technique combined with first-principles theory. Cross-sectional TEM images reveal the BiFeO3 film consists of two zones with different crystal structures...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46498
更新日期：2017-04-19 00:00:00
abstract：:Coral reefs face a crisis due to local and global anthropogenic stressors. A large proportion of the ~50% coral loss on the Great Barrier Reef has been attributed to outbreaks of the crown-of-thorns-seastar (COTS). A widely assumed cause of primary COTS outbreaks is increased larval survivorship due to higher food ava...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08402
更新日期：2015-02-12 00:00:00
abstract：:Chlorella is a unicellular green microalga that has been used in fields such as bioenergy production and food supplementation. In this study, two promoters of N (nitrogen) deficiency-inducible Chlorella vulgaris N Deficiency Inducible (CvNDI) genes were isolated from Chlorella vulgaris UTEX 395. These promoters were u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69620-9
更新日期：2020-07-29 00:00:00
abstract：:The apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) family proteins bind RNA and single-stranded DNA, and create C-to-U base modifications through cytidine deaminase activity. APOBEC3G restricts human immunodeficiency virus 1 (HIV-1) infection by creating hypermutations in proviral DNA, while HIV-1-e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40783
更新日期：2017-01-18 00:00:00
abstract：:Nitric oxide (NO) signaling has been studied in the eye, including in the pathophysiology of some eye diseases. While NO production by nitric oxide synthase (NOS) enzymes in the eye has been characterized, the more recently described pathways of NO generation by nitrate (NO3-) and nitrite (NO2-) ions reduction has rec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69272-9
更新日期：2020-08-05 00:00:00
abstract：:The growth in energy devices and the role of supercapacitors are increasingly important in today's world. Designing an electrode material for supercapacitors using metals that have high performance, superior structure, are eco-friendly, inexpensive and highly abundant is essentially required for commercialization. In ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75879-9
更新日期：2020-11-05 00:00:00
abstract：:Rodents are important reservoir hosts of many important zoonotic viruses. The family Picornaviridae contains clinically important pathogens that infect humans and animals, and increasing numbers of rodent picornaviruses have recently been associated with zoonoses. We collected 574 pharyngeal and anal swab specimens fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34381
更新日期：2016-09-29 00:00:00
abstract：:Over the last 5-10 years, optical coherence tomography (OCT) and atomic force microscopy (AFM) have been individually applied to monitor the morphological and mechanical properties of various single-species biofilms respectively. This investigation looked to combine OCT and AFM as a multi-scale approach to understand ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23798-1
更新日期：2018-04-09 00:00:00
abstract：:Efficient hematopoietic reconstitution of wild type mice requires preconditioning. Established experimental protocols exist to transplant hematopoietic stem cells into lethally irradiated or chemically myeloablated adult mice or unirradiated immunodeficient mice. We sought to develop a protocol to reconstitute immuno-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30767-1
更新日期：2018-08-16 00:00:00
abstract：:Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase that mediates phosphoinositide-3-kinase (PI3K)/AKT signalling. This pathway is involved in a plethora of cellular functions including protein and lipid synthesis, cell migration, cell proliferation and apoptosis. In this study, we proposed to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53141-1
更新日期：2019-11-15 00:00:00
abstract：:Polymer derived ceramic (PDC) composite coatings were deposited on AISI 304 substrates using siloxane based preceramic polymer polymethlysilsquioxane (PMS) and ZrSi2 as active filler or Ag as passive filler. The tribological performance of the composite coatings was evaluated at room temperature and moderately high te...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33441-8
更新日期：2018-10-10 00:00:00
abstract：:The development of human induced pluripotent stem cell (iPSC) technology has revolutionized the regenerative medicine field. This technology provides a powerful tool for disease modeling and drug screening approaches. To circumvent the risk of random integration into the host genome caused by retroviruses, non-integra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08081
更新日期：2015-01-28 00:00:00
abstract：:CaWRKY40 is a positive regulator of pepper (Capsicum annum) response to Ralstonia solanacearum inoculation (RSI), but the underlying mechanism remains largely unknown. Here, we functionally characterize CaCDPK15 in the defense signaling mediated by CaWRKY40. Pathogen-responsive TGA, W, and ERE boxes were identified in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22439
更新日期：2016-03-01 00:00:00
abstract：:Humans interact with the oceans in diverse and profound ways. The scope, magnitude, footprint and ultimate cumulative impacts of human activities can threaten ocean ecosystems and have changed over time, resulting in new challenges and threats to marine ecosystems. A fundamental gap in understanding how humanity is af...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47201-9
更新日期：2019-08-12 00:00:00
abstract：:We report a multi-channel optical frequency synthesizer developed to generate extremely stable continuous-wave lasers directly out of the optical comb of an Er-doped fiber oscillator. Being stabilized to a high-finesse cavity with a fractional frequency stability of 3.8 × 10-15 at 0.1 s, the comb-rooted synthesizer pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44122-5
更新日期：2019-05-21 00:00:00
abstract：:Impaired wound healing is an immense medical challenge, and while autologous skin grafting remains the "gold-standard" therapeutic option for repairing wounds that cannot be closed by primary or secondary intention, it is limited by substantial donor site morbidity. We previously developed the alternative approach of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81179-7
更新日期：2021-01-18 00:00:00
abstract：:Using symbiotic farming methods [cover crops and chicken grazing (+ C)] in a winter fallow field, we found that the soil organic matter and total nitrogen of the + C treatment were 5.2% and 26.6% higher, respectively, than those of a treatment with cover crops and no chicken grazing (- C). The annual rice grain yield ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69407-y
更新日期：2020-07-28 00:00:00
