Abstract：
INTRODUCTION:MicroRNAs (miRs) are a family of noncoding RNAs that regulate gene expression. They are ubiquitous among multicellular eukaryotes and are also encoded by some viruses. Upon infection, viral miRs (vmiRs) can potentially target gene expression in the host and alter the immune response. Although prior studies have reported viral infections in human pulp, the role of vmiRs in pulpal disease is yet to be explored. The purpose of this study was to examine the expression of vmiRs in normal and diseased pulps and to identify potential target genes. METHODS:Total RNA was extracted and quantified from normal and inflamed human pulps (N = 28). Expression profiles of vmiRs were then interrogated using miRNA microarrays (V3) and the miRNA Complete Labeling and Hyb Kit (Agilent Technologies, Santa Clara, CA). To identify vmiRs that were differentially expressed, we applied a permutation test. RESULTS:Of the 12 vmiRs detected in the pulp, 4 vmiRs (including those from herpesvirus and human cytomegalovirus) were differentially expressed in inflamed pulp compared with normal pulp (P < .05). Using bioinformatics, we identified potential target genes for the differentially expressed vmiRs. They included key mediators involved in the detection of microbial ligands, chemotaxis, proteolysis, cytokines, and signal transduction molecules. CONCLUSIONS:These data suggest that miRs may play a role in interspecies regulation of pulpal health and disease. Further research is needed to elucidate the mechanisms by which vmiRs can potentially modulate the host response in pulpal disease.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Zhong S,Naqvi A,Bair E,Nares S,Khan AAdoi
10.1016/j.joen.2016.10.006subject
Has Abstractpub_date
2017-01-01 00:00:00pages
84-89issue
1eissn
0099-2399issn
1878-3554pii
S0099-2399(16)30733-6journal_volume
43pub_type
杂志文章abstract：INTRODUCTION:The purpose of this study was to compare the accuracy in vivo of 2 electronic apex locators (EALs), the Raypex 5 and the Mini Apex Locator. METHODS:The working length (WL) was determined electronically for 40 human root canals by using a K-file and 1 of the 2 EALs. The files were fixed at the WL, and the ...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.06.021
更新日期：2011-10-01 00:00:00
abstract：:Ninety-six human molars were accessed. After flattening of the occlusal surface and removal of the apical two-thirds of the roots, the remaining canal space was instrumented by conventional methods. The teeth were divided into six groups of 15 teeth with three positive and three negative controls. The six experimental...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80078-6
更新日期：1994-12-01 00:00:00
abstract：INTRODUCTION:Nonequilibrium plasma has been reported to effectively kill Enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (CHX). The purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with CHX against E. faecalis and multispecie...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.06.027
更新日期：2013-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate and compare the centering ability and canal transportation of the ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland) and Self-adjusting File (SAF; ReDent-Nova, Ra'anana, Israel) systems in long oval root canals using cone-beam computed tomography imag...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.07.007
更新日期：2019-10-01 00:00:00
abstract：INTRODUCTION:Many studies have investigated the morphology of the mandibular molar, but the prevalence of the middle mesial (MM) canal in the mesial root of the mandibular molar is still the subject of controversy. In addition, in previous literature, a true MM canal has not been clearly distinguished from an isthmus b...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.02.008
更新日期：2017-07-01 00:00:00
abstract：:The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, e...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200212000-00006
更新日期：2002-12-01 00:00:00
abstract：INTRODUCTION:The purpose of this retrospective study was to determine the success of the inferior alveolar nerve (IAN) block using either 3.6 mL or 1.8 mL 2% lidocaine with 1:100,000 epinephrine in patients presenting with symptomatic irreversible pulpitis. METHODS:As part of 7 previously published studies, 319 emerge...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2013.04.007
更新日期：2013-08-01 00:00:00
abstract：:This study compared the quality of obturation of high- and low-temperature thermoplasticized injectable gutta-percha techniques and standard lateral condensation. A new model system was developed to more closely simulate the clinical environment. All obturations were performed in this same model which allowed direct c...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)81863-7
更新日期：1991-06-01 00:00:00
abstract：INTRODUCTION:The purpose of the study was to evaluate the effects of intracanal, intraoral, and extraoral cryotherapy on postoperative pain in molar teeth with symptomatic apical periodontitis. METHODS:A total of 100 patients were randomly distributed into 4 groups: control (without cryotherapy application), intracana...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2017.11.002
更新日期：2018-03-01 00:00:00
abstract：:Twenty volunteers with first molars free of restorations and caries were recruited. One molar from each arch was selected, and rubber dam was applied. Seven sites on each crown were electric pulp tested 4 times with an Elements Diagnostic Unit, and lowest threshold responses were recorded. Data were analyzed with one-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.08.006
更新日期：2007-11-01 00:00:00
abstract：:A descriptive, cross sectional survey was developed to determine the preferences of endodontists when prescribing analgesics. Eleven clinical scenarios describing common endodontic diagnoses or procedures with specified severity of pain were provided. A survey was sent to 310 AAE members and 63 responded, providing a ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2006.07.015
更新日期：2006-12-01 00:00:00
abstract：:The role of T-cells in the development of periapical lesions was investigated immunohistochemically using 16 normal (BALB/c) mice and 16 nude (BALB/c nu/nu) mice (congenitally T-cell-deficient mice). The pulp chambers of maxillar first molars of all mice were opened, and the infiltrated immunocytes (anti-Thy1.2, -Lyt-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(06)80565-0
更新日期：1995-04-01 00:00:00
abstract：:This study is a quantitative assessment of the luminous energy transmitted through different translucent fiber posts. After embedding the posts in black resin, the blocks were submitted to sequential cuts in a precision machine, and depths of 16 mm, 12 mm, 8 mm, and 4 mm were assessed for light transmission with a dig...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2007.12.007
更新日期：2008-03-01 00:00:00
abstract：INTRODUCTION:During apicoectomy and retrograde cavity preparation, a smear layer, which contains microorganisms and necrotic pulpal tissues, is formed on the dentinal surfaces cut by the instruments. Bacteria can survive and proliferate inside or below the smear layer. The purpose of this study was to evaluate in vitro...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2011.03.001
更新日期：2011-06-01 00:00:00
abstract：:The state of contamination by methicillin-resistant Staphylococci, including methicillin-resistant Staphylococcus aureus (MRSA) at the conservative dentistry section of the School of Dentistry, Aichi-Gakuin University, was investigated. Methicillin-resistant coagulase-negative Staphylococci (MRCNS) were detected in 8 ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(06)80552-2
更新日期：1995-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the number of canals and isthmuses obturated after Cr, Er: YSGG laser treatment. Fifty-two canals were instrumented to a size 40 Profile 0.06 file combined with RC-Prep and sodium hypochlorite, or sterile water. The teeth were divided into 3 groups: (I) canals were flooded wit...
journal_title：Journal of endodontics
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joen.2007.05.012
更新日期：2007-09-01 00:00:00
abstract：INTRODUCTION:Mineral trioxide aggregate (MTA) satisfies most of the ideal properties of a surgical root-end filling and perforation repair material. It has been found to be nontoxic, noncarcinogenic, nongenotoxic, biocompatible, insoluble in tissue fluids, and dimensionally stable and promotes cementogenesis. The major...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：INTRODUCTION:Calcium hydroxide has been used as a traditional pulpotomy agent for a long time but has some disadvantages. iRoot BP Plus (Innovative Bioceramix Inc, Vancouver, Canada) is a newly developed, ready-to-use calcium silicate-based bioactive ceramic with excellent bioactivity and sealing ability. However, whet...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2019.12.010
更新日期：2020-03-01 00:00:00
abstract：INTRODUCTION:Vertical root fractures (VRFs) are among the most frequent causes of tooth loss, mainly of endodontically treated teeth. However, very few data is available about the occurrence of VRFs following apical surgery. METHODS:Patient charts from 864 patients with 1058 teeth treated with apical surgery (Septembe...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2020.10.012
更新日期：2021-02-01 00:00:00
abstract：:Dentinal chips and different biocompatible materials have been used in the past as apical plugs to enhance healing and simplify obturation. The purpose of this study was to evaluate bone reactions to implants of hydroxyapatite, collagen, and calcium hydroxide, alone or in different combinations. The materials were imp...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/s0099-2399(07)80175-0
更新日期：1990-10-01 00:00:00
abstract：INTRODUCTION:The purpose of this study was to evaluate the accuracy of cone-beam computed tomographic (CBCT) to measure dentin thickness and its potential of predicting the remaining dentin thickness after the removal of fractured instrument fragments. METHODS:Twenty-three human mandibular molars were selected, and 4-...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.03.041
更新日期：2017-09-01 00:00:00
abstract：:Twenty-one teeth with clinically asymptomatic periapical pathosis (class 3) were extracted and the isolation, identification, and localization of bacteria in the root apex were examined. Mixtures involving several bacteria were isolated from more than 60% of the cases. Scanning electron microscopy revealed bacterial m...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(07)80216-0
更新日期：1990-11-01 00:00:00
abstract：:The polymerase chain reaction (PCR) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. The purpose of this study was to use PCR to detect the presence of specific species of bacteria in samples collected from two geographical locat...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1097/00004770-200403000-00004
更新日期：2004-03-01 00:00:00
abstract：INTRODUCTION:This study evaluated the cyclic fatigue resistance (CFR) of Reciproc (R25 and R40; VDW, Munich, Germany) and Reciproc Blue (R25 and R40, VDW) instruments used in an artificial S-shaped canal. METHODS:A total of 80 files were tested in an S-shaped canal (n = 20 for each file, Reciproc R25 and R40 and Recip...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2017.04.009
更新日期：2017-10-01 00:00:00
abstract：:The antimicrobial effect of RC-Prep and its components--10% urea peroxide, 15% EDTA, and glycol--were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent fo...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/S0099-2399(99)80296-9
更新日期：1999-12-01 00:00:00
abstract：INTRODUCTION:The efficacy of retreatment techniques for BC Sealer (BCS) (Brasseler USA, Savannah, GA) removal has not yet been assessed. The purpose of this study was to evaluate the efficacy of solvent and rotary instrumentation in the removal of BCS when used in combination with gutta-percha (GP) as compared with AH ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：INTRODUCTION:Fusion is a rare occurrence, and its definitive diagnosis is of prime importance for successful root canal treatment. This case report discusses the endodontic management of a supernumerary tooth fused with a right maxillary first molar by using cone-beam computed tomography (CBCT). METHODS:Nonsurgical en...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2010.08.026
更新日期：2010-11-01 00:00:00
abstract：INTRODUCTION:The purpose of this in vitro study was to compare the cytotoxicity of white mineral trioxide aggregate cement (AMTA, MTA-Angelus), Brasseler Endosequence Root Repair Putty (ERRM), Dycal, and Ultra-blend Plus (UBP) by using human dermal fibroblasts and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.11.012
更新日期：2012-03-01 00:00:00
abstract：INTRODUCTION:The study compared Twisted File (TF) with 3 traditionally manufactured systems to determine whether changes in the manufacturing process improved the cyclic fatigue resistance. METHODS:Four rotary file systems, (1) ProFile (PF), (2) Mtwo, (3) K3, and (4) TF, were tested in artificial canals with 45° and 9...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2011.12.034
更新日期：2012-04-01 00:00:00
abstract：INTRODUCTION:Different single-file systems are available for endodontic treatment; however, comparative studies are scarce. Thus, the present study evaluated bacterial reduction promoted by 2 single-file systems: Wave One (Dentsply Maillefer, Ballaigues, Switzerland) and One Shape (Micromega, Besancon, France). METHOD...
journal_title：Journal of endodontics
pub_type： 杂志文章
doi：10.1016/j.joen.2014.07.024
更新日期：2014-12-01 00:00:00
