Abstract：
:To study directly the functions of the cells that line the bile ducts inside the liver, we developed a new technique for isolating intrahepatic bile duct epithelial cells (IBDECs) from normal rat liver. Parenchymal and nonparenchymal cells were separated from whole liver by enzymatic digestion and mechanical disruption; subpopulations of individual nonparenchymal cells then were isolated by serial counter-flow elutriation, isopycnic centrifugation, and immunoaffinity separation with a specific monoclonal antibody against an antigen on the plasma membrane of IBDECs. Using this approach, we isolated 1.2 +/- 0.2 x 10(6) (mean +/- SE) viable (greater than 95% trypan blue exclusion) cells, greater than 95% of which were identified as IBDECs by morphologic appearance and specific cytochemical markers. The IBDECs averaged 7.4 +/- 0.16 microM in diameter and retained their in situ appearance, including morphologic polarity. They appeared as single cells or as cell doublets attached by tight junctions that excluded ruthenium red. Microvilli were abundant and were restricted to the apical (i.e., luminal) domain of the plasma membrane. Coated pits were observed on both apical and basolateral cell surfaces. Internally, IBDECs contained a well-developed system of organelles, including mitochondria, Golgi, and discrete types of vesicles, such as coated vesicles, multivesicular bodies, and lysosomes. These results indicate that a highly purified suspension of viable, morphologically intact, and polar IBDECs can be prepared from normal rat liver using a novel approach that separates liver cells on the basis of size, density, and specific membrane components. The availability of such a model will allow experimental studies to be performed directly on IBDECs, an approach that has not previously been possible.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Ishii M,Vroman B,LaRusso NFdoi
10.1016/0016-5085(89)91695-8subject
Has Abstractpub_date
1989-11-01 00:00:00pages
1236-47issue
5eissn
0016-5085issn
1528-0012pii
0016-5085(89)91695-8journal_volume
97pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：BACKGROUND & AIMS:Agents designed to block or alter cytokinesis can kill or stop proliferation of cancer cells. We aimed to identify cytokinesis-related proteins that are overexpressed in hepatocellular carcinoma (HCC) cells and might be targeted to slow liver tumor growth. METHODS:Using the Oncomine database, we comp...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2018.07.010
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND & AIMS:Elevated hepatic iron concentration may affect the response to antiviral therapy in chronic hepatitis C. This study explored the contribution of genetic hemochromatosis to iron accumulation in chronic hepatitis C. METHODS:HFE mutations (C282Y and H63D) were assessed in 184 patients with chronic hepat...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(99)70236-2
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND & AIMS:Despite inducing an inflammatory response, Helicobacter pylori can persist in the gastric mucosa for decades. H pylori expression of cholesterol-α-glucosyltransferase (encoded by cgt) is required for gastric colonization and T-cell activation. We investigated how cgt affects gastric epithelial cells a...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2017.12.008
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatic osmoreceptors are sensitive to changes in portal blood osmolality and cause variations in plasma antidiuretic hormone and water diuresis, which prevent major systemic osmotic changes. Insensitivity of hepatic osmoreceptors might contribute to maintaining increased plasma levels of antidiuretic h...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(94)90702-1
更新日期：1994-03-01 00:00:00
abstract：:Upper gut transit and motility among 10 symptomatic and 9 asymptomatic patients with Roux gastrectomy were compared with those among 10 healthy, unoperated controls. Gastric emptying of solids and Roux limb and small intestinal transit of liquids were assessed scintigraphically. Motor patterns in the Roux limb or heal...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(92)91496-q
更新日期：1992-10-01 00:00:00
abstract：BACKGROUND/AIMS:Ursodeoxycholic acid (UDCA) and colchicine have beneficial effects in primary biliary cirrhosis (PBC). The efficacy of colchicine and UDCA in PBC was compared in a 2-year placebo-controlled study (n = 90). METHODS:Clinical events, laboratory test results, and liver histology were recorded at the beginn...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/0016-5085(95)90696-7
更新日期：1995-05-01 00:00:00
abstract：BACKGROUND & AIMS:Simvastatin improves liver generation of nitric oxide and hepatic endothelial dysfunction in patients with cirrhosis, so it could be an effective therapy for portal hypertension. This randomized controlled trial evaluated the effects of continuous simvastatin administration on the hepatic venous press...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1053/j.gastro.2009.01.043
更新日期：2009-05-01 00:00:00
abstract：:Previous studies suggested that the food-containing canine stomach retains large, nondigestible spheres until all food has emptied; but it is not known whether there is a threshold size or a gradation of sizes that will empty along with food. Further, nothing is known of the effects of such parameters as density, shap...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(85)90576-1
更新日期：1985-10-01 00:00:00
abstract：:We have previously demonstrated circulating and tissue-fixed immune complexes containing the small cytoplasmic ribonucleoprotein antigen Ro and immunoglobulin G (IgG) in a case of sicca syndrome associated with primary biliary cirrhosis. To establish the overall prevalence of such complexes in liver disease, we perfor...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(86)91129-7
更新日期：1986-03-01 00:00:00
abstract：BACKGROUND & AIMS:During severe pancreatitis, interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha are produced in large quantities. The aim of this study was to determine whether either one plays a more dominant role and if their detrimental effects are additive. METHODS:Necrotizing pancreatitis was induced i...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.1997.v113.pm9352880
更新日期：1997-11-01 00:00:00
abstract：BACKGROUND & AIMS:Accurate biomarkers of disease activity and therapeutic response can be valuable for clinical trials. We performed a post hoc analysis of data from a phase 2 trial to assess the relationship between the concentration of fecal calprotectin (FCP) and clinical and endoscopic outcomes of patients with mod...
journal_title：Gastroenterology
pub_type： 杂志文章,随机对照试验
doi：10.1053/j.gastro.2015.09.001
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND & AIMS:Esophageal adenocarcinoma (EA) and its premalignant lesion, Barrett's esophagus (BE), are characterized by a strong and yet unexplained male predominance (with a male-to-female ratio in EA incidence of up to 6:1). Genome-wide association studies (GWAS) have identified more than 20 susceptibility loci ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2020.08.052
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND & AIMS:The goal of the study was to facilitate fat balance measurements with an appropriate intestinal marker for the transit of dietary fat and thereby eliminate the need for complete diet and fecal collections. METHODS:Dietary fat containing 5% sucrose polybehenate was fed in a semisynthetic diet to rats ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2004.04.007
更新日期：2004-07-01 00:00:00
abstract：BACKGROUND & AIMS:The effects of short-term antigenic activation of mast cells on the gastrointestinal tract have been well characterized, but little is known about the effects of long-term exposure to antigen on mucosal mast cell reactivity. The aim of this study was to determine the effects of long-term antigen expos...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(96)70013-6
更新日期：1996-12-01 00:00:00
abstract：BACKGROUND & AIMS:In patients with chronic Helicobacter pylori (H pylori) infection, parietal and chief cell atrophy in the gastric corpus, a process known as spasmolytic polypeptide-expressing metaplasia (SPEM), increases the risk for progression to cancer. The relation between H pylori and these metaplastic changes i...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2018.09.050
更新日期：2019-01-01 00:00:00
abstract：:Our objectives in this study were (a) to determine the role of antroduodenal resistance in the control of fasting duodenogastric bile reflux in the dog and (b) to elucidate the contribution of the pylorus both to resistance and to reflux. Thus, we measured simultaneously throughout the interdigestive motor cycle (a) a...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(87)90566-x
更新日期：1987-11-01 00:00:00
abstract：:In dogs provided with Heidenhain pouch and gastric fistula, we studied the effect of near-total excision of the fundic mucosa of the main stomach on acid secretion and gastrin release. Fundic mucosal excision abolished the mean response of the gastric fistula and reduced its maximal response to pentagastrin and to his...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：BACKGROUND & AIMS:Little is known about substitutions that mediate resistance of hepatitis C virus (HCV) to direct-acting antivirals (DAAs), due to the small number of patients with treatment failure in approval studies. It is important to identify resistance patterns to select effective salvage treatments. METHODS:We...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究
doi：10.1053/j.gastro.2017.11.007
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND & AIMS:During hepatic fibrogenesis, the hepatic extracellular matrix changes to fibrillar collagens types I and III, and cirrhosis is believed to produce an irreversible scar. In this study, we investigated whether gene delivery of human matrix metalloproteinase-1, which degrades collagens types I and type I...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2003.50063
更新日期：2003-02-01 00:00:00
abstract：BACKGROUND & AIMS:Cure of Helicobacter pylori infection may lead to complete remission of associated low-grade mucosa-associated lymphoid tissue (MALT) lymphoma in stage EI. This study investigated whether Helicobacter heilmannii infection-associated primary gastric MALT lymphoma will regress after cure of the infectio...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章
doi：10.1016/s0016-5085(00)70167-3
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND & AIMS:Oral linaclotide, a novel agonist of guanylate cylase-C, stimulates intestinal fluid secretion and transit, and decreases visceral hypersensitivity in animal studies. In healthy volunteers, linaclotide was safe, well tolerated, increased stool frequency, and decreased stool consistency and time to fir...
journal_title：Gastroenterology
pub_type： 杂志文章,随机对照试验
doi：10.1053/j.gastro.2007.06.067
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND & AIMS:Two percent to 4% of all cases of colorectal cancer (CRC) are associated with Lynch syndrome. Dominant clustering of CRC (non-Lynch syndrome) accounts for 1%-3% of the cases. Because carcinogenesis is accelerated in Lynch syndrome, an intensive colonoscopic surveillance program has been recommended si...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2010.02.053
更新日期：2010-06-01 00:00:00
abstract：:In neurogenic fecal incontinence there is denervation of the external anal sphincter and pelvic floor muscles but the role of the internal anal sphincter is incompletely understood. We have evaluated the internal anal sphincter in 6 patients with neurogenic incontinence undergoing postanal repair and in 7 control subj...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(88)90175-8
更新日期：1988-10-01 00:00:00
abstract：BACKGROUND & AIMS:The optimal strategy for the detection of hereditary nonpolyposis colorectal cancer (HNPCC) gene carriers remains uncertain. We evaluated whether microsatellite instability (MSI) analysis or MSH2 and MLH1 protein immunostaining of tumors will screen individuals efficiently for germline MSH2 and MLH1 t...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2001.20874
更新日期：2001-01-01 00:00:00
abstract：:Several prostaglandins are cytoprotective for the stomach; they prevent mucosal necrosis and hemorrhages produced by noxious agents, such as absolute ethanol. One possible mechanism of cytoprotection would be that the prostaglandin may prevent penetration of the necrotizing agent into the gastric mucosa. To test this ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(85)80187-6
更新日期：1985-01-01 00:00:00
abstract：:Gastrinomas were localized by concurrent blood sampling in the hepatic vein and portal vein tributaries in 10 of 12 Zollinger-Ellison patients. In one patient, the portal vein could not be catheterized; in the other, a gastrin gradient could not be picked up in either of two examinations. Six of the patients were subs...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：BACKGROUND & AIMS:Activating transcription factor 4 (ATF4) regulates genes involved in the inflammatory response, amino acid metabolism, autophagy, and endoplasmic reticulum stress. We investigated whether its activity is altered in patients with inflammatory bowel diseases (IBDs) and mice with enterocolitis. METHODS:...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2018.11.033
更新日期：2019-03-01 00:00:00
abstract：:A 60-year-old white male presenting with a clinical picture of obstructive jaundice was subsequently found to have erythropoietic protoporphyria. The diagnosis was suspected because of a history of life-long photosensitivity and was confirmed by finding high levels of erythrocyte protoporphyrin. Liver biopsy revealed ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:With a worldwide prevalence of 15%, chronic constipation is one of the most frequent gastrointestinal diagnoses made in ambulatory medicine clinics, and is a common source cause for referrals to gastroenterologists and colorectal surgeons in the United States. Symptoms vary among patients; straining, incomplete evacua...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1053/j.gastro.2019.12.034
更新日期：2020-04-01 00:00:00
abstract：:The prevalence of gastroesophageal varices and gastrointestinal hemorrhage was determined in 124 patients with severe chronic active liver disease (CALD) receiving prednisone and followed regularly for up to 10 yr. Varices were demonstrated by contrast radiography in only 19 patients (15%). Ten had varices before ther...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
