Abstract:
:We have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 μm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C18-groups, for separating a wide range of molecules (from small metabolites to intact proteins). Compared to today's capillary/nanoLC approaches, our system provides significantly enhanced sensitivity (up to several orders) with matching or improved separation efficiency, and highly repeatable chromatographic performance. The chemical properties of the trap column and the analytical column were fine-tuned to obtain practical sample loading capacities (above 2 μg), an earlier bottleneck of OTLC. Using the OTLC system (combined with Orbitrap mass spectrometry), we could perform targeted metabolomics of sub-μg amounts of exosomes with 25 attogram detection limit of a breast cancer-related hydroxylated cholesterol. With the same set-up, sensitive bottom-up proteomics (targeted and untargeted) was possible, and high-resolving intact protein analysis. In contrast to state-of-the-art packed columns, our platform performs chromatography with very little dilution and is "fit-for-all", well suited for comprehensive analysis of limited samples, and has potential as a tool for challenges in diagnostics.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Vehus T,Roberg-Larsen H,Waaler J,Aslaksen S,Krauss S,Wilson SR,Lundanes Edoi
10.1038/srep37507subject
Has Abstractpub_date
2016-11-29 00:00:00pages
37507issn
2045-2322pii
srep37507journal_volume
6pub_type
杂志文章abstract::We report a modified two-dimensional Cherenkov radiation, which occurs on a high-index dielectric nano-film driven by uniformly moving electron-beam. It is essentially different from the ordinary Cherenkov radiation in that, in the nondispersive medium, it shows unique dispersion characteristics-the waves with higher ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06176-1
更新日期:2017-07-19 00:00:00
abstract::Mutations in the SNX14 gene cause spinocerebellar ataxia, autosomal recessive 20 (SCAR20) in both humans and dogs. Studies implicating the phenotypic consequences of SNX14 mutations to be consequences of subcellular disruption to autophagy and lipid metabolism have been limited to in vitro investigation of patient-der...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70797-2
更新日期:2020-08-13 00:00:00
abstract::Progression of age can influence gait characteristics. Previous research has investigated lower extremity joint mechanics between young and elderly people in locomotion, however little is known about whether differences exist between young and middle age people. Ten young healthy subjects (22.8 ± 5.3 years) and ten mi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41750-9
更新日期:2019-04-03 00:00:00
abstract::Hearing loss becomes increasingly common with age and affects quality of life. Recently, scientists have published articles about the relationship between metabolic disease and hearing loss. Metabolic disease was previously found to be highly related to an increase in alkaline phosphatase. Thus, there may be an indire...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/s41598-020-60979-3
更新日期:2020-03-04 00:00:00
abstract::The hyaluronan synthase 2 (HAS2)-hyaluronic acid (HA)-CD44-Caspase-3 pathway is involved in ovarian granulosa cell (GC) functions in mammals. HAS2 is a key enzyme required for HA synthesis and is the key factor in this pathway. However, the regulation of HAS2 and the HAS2-mediated pathway by microRNAs in GCs is poorly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21197
更新日期:2016-02-18 00:00:00
abstract::Particle shape plays a crucial role in determining packing characteristics. Real particles in nature usually have rounded corners. In this work, we systematically investigate the rounded corner effect on the dense packings of spherotetrahedral particles. The evolution of dense packing structure as the particle shape c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15640
更新日期:2015-10-22 00:00:00
abstract::Hetero-nanostructures based on magnetic contrast oxides have been prepared as highly dense nanoconsolidates. Cobalt ferrite-cobalt oxide core-shell type nanoparticles (NPs) were synthesized by seed mediated growth in polyol and subsequently consolidated by Spark Plasma Sintering (SPS) at 500 °C for a few minutes while...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55649-y
更新日期:2019-12-19 00:00:00
abstract::RNA-binding proteins are key regulators of post-transcriptional processes such as alternative splicing and mRNA stabilization. Rbm24 acts as a regulator of alternative splicing in heart and skeletal muscle, and is essential for sarcomere assembly. Homozygous inactivation of Rbm24 in mice disrupts cardiac development a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64667-0
更新日期:2020-05-06 00:00:00
abstract::Advanced glycation end products (AGEs) play a key role in the progression of heart failure. Whether treatments limiting AGEs formation would prevent adverse left ventricular remodeling after myocardial infarction (MI) remain unknown. We investigated whether pyridoxamine (PM) could limit adverse cardiac outcome in MI. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16255-y
更新日期:2017-11-22 00:00:00
abstract::Levels of neutral poly-/perfluoroalkyl substances (nPFASs) in air and snow collected from Ny-Ålesund were measured and their air-snow exchange was determined to investigate whether they could re-volatilize into the atmosphere driven by means of air-snow exchange. The total concentration of 12 neutral PFASs ranged from...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08912
更新日期:2015-03-09 00:00:00
abstract::Although N-acyl-L-homoserine lactone (AHL) based quorum sensing (QS) phenomenon has been observed in mature anaerobic granules, the biological role of AHL-based QS system in anaerobic granulation process remains unexplored. For the first time, a long-term anaerobic bioreactor was operated for 168 days to investigate t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34183-3
更新日期:2018-10-25 00:00:00
abstract::Mechanistic disease progression studies using animal models require objective and quantifiable assessment of tissue pathology. Currently quantification relies heavily on staining methods which can be expensive, labor/time-intensive, inconsistent across laboratories and batch, and produce uneven staining that is prone ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78061-3
更新日期:2020-12-01 00:00:00
abstract::Iron deposits are a phenotypic trait of tumor-associated macrophages (TAMs). Histological iron imaging and contrast-agent free magnetic resonance imaging (MRI) can detect these deposits, but their presence in human cancer, and correlation with immunotherapeutic response is largely untested. Here, primarily using thes...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37408-7
更新日期:2019-01-29 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-59358-9
更新日期:2020-02-05 00:00:00
abstract::Medication-related osteonecrosis of the jaw (MRONJ) represents a complication of bisphosphonate treatment that responds poorly to standard treatment. In a retrospective cohort study we investigated a possible role of Actinomyces spp. in the pathogenesis of MRONJ. Deep biopsies of necrotic bone were collected during su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31604
更新日期:2016-08-17 00:00:00
abstract::The effective differentiation between multiple primary lung tumors (MPs) and intrapulmonary metastases (IMs) in patients is imperative to discover the exact disease stage and to select the most appropriate treatment. In this study, the authors was to evaluate the efficacy and validity of large-scale targeted sequencin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75935-4
更新日期:2020-11-02 00:00:00
abstract::We developed an optimal noninvasive index comprising routine laboratory parameters for predicting cirrhosis in chronic hepatitis B (CHB) and chronic hepatitis C (CHC) patients. This study included 992 CHB patients and 1,284 CHC patients who received liver biopsy. We developed the new index, named modified Fibrosis-4 (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03589-w
更新日期:2017-06-12 00:00:00
abstract::Antimicrobial peptides, produced by innate immune system of hosts in response to invading pathogens, are capable of fighting against a spectrum of bacteria, viruses, fungi, parasites and cancer cells. Here, a recombinant silkworm AMP Bmattacin2 from heterologous expression is studied, indicating a broad spectrum of an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31149
更新日期:2016-08-09 00:00:00
abstract::Here, water flow inside large radii semi-infinite carbon nanotubes is investigated. Permeable wall taking into account the molecular interactions between water and a nanotube, and the slip boundary condition will be considered. Furthermore, interactions among molecules are approximated by the continuum approximation. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48614-2
更新日期:2019-08-20 00:00:00
abstract::A frequency reconfigurable dipole antenna based on a silicon radiator is presented. The silicon radiator is activated with the aid of highly dense solid-state plasma by injecting carriers into the intrinsic region of p-i-n diodes. The fabrication and design guideline of the reconfigurable dipole antenna with this plas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33278-1
更新日期:2018-10-09 00:00:00
abstract::Either hardness or toughness has been the core interest in scientific exploration and technological pursuit for a long time. However, it is still a big challenge to enhance the hardness and toughness at the same time, since the improvement of one side is always at the expense of the other one. Here, we have succeeded ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09807-9
更新日期:2017-08-24 00:00:00
abstract::The role of extractable pool of biochar in crop productivity and soil greenhouse gas (GHGs) emission is not yet clear. In this study, two biochars with and without extraction was added to a paddy before rice transplantation at 20 t·ha-1. Crop yield, plant traits and greenhouse gas emission monitored throughout a rice-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19331-z
更新日期:2018-01-16 00:00:00
abstract::Kidney regeneration from pluripotent stem cells is receiving a lot of attention because limited treatments are currently available for chronic kidney disease (CKD). It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells, such as endothelial progenitor and mesenchymal stem cells, affecting...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33256-7
更新日期:2018-10-08 00:00:00
abstract::Knowledge of the ecology of N2-fixing (diazotrophic) plankton is mainly limited to oligotrophic (sub)tropical oceans. However, diazotrophs are widely distributed and active throughout the global ocean. Likewise, relatively little is known about the temporal dynamics of diazotrophs in productive areas. Between February...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39586-4
更新日期:2019-03-06 00:00:00
abstract::Current preclinical screening methods do not adequately detect cardiotoxicity. Using human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs), more physiologically relevant preclinical or patient-specific screening to detect potential cardiotoxic effects of drug candidates may be possible. However, one of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11817
更新日期:2015-07-03 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-42195-w
更新日期:2019-04-10 00:00:00
abstract::Based on an inverted bulk band order, antimony thin films presumably could become topological insulators if quantum confinement effect opens up a gap in the bulk bands. Coupling between topological surface states (TSS) from opposite surfaces, however, tends to degrade or even destroy their novel characters. Here the e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02010
更新日期:2013-01-01 00:00:00
abstract::A cross-correlative precession electron diffraction - atom probe tomography investigation of Cr segregation in a Fe(Cr) nanocrystalline alloy was undertaken. Solute segregation was found to be dependent on grain boundary type. The results of which were compared to a hybrid Molecular Dynamics and Monte Carlo simulation...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34642
更新日期:2016-10-06 00:00:00
abstract::Crossmodal studies have reported not only facilitatory but also inhibitory perceptual interactions. For instance, tactile stimulation to the index finger of a hand leads to the degradation of visual discrimination performance (touch-induced visual suppression, TIVS). It has been suggested that the magnitude of TIVS de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35302-w
更新日期:2018-11-19 00:00:00
abstract::Cd3As2 has long been known as a high-mobility semiconductor. The recent finding of a topological semimetal state in this compound has demanded growth of epitaxial films with high crystallinity and controlled thickness. Here we report the structural characterisation of Cd3As2 films grown on SrTiO3 substrates by solid-p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20758-7
更新日期:2018-02-02 00:00:00