Abstract:
:Ununited peripheral nerves represent attractive site for connectivity with neuroprostheses because their predictable internal topography allows precise sorting of motor and sensory signals. Also transplantation of bone marrow mesenchymal stem cells (BMSCs) is increasingly recognized as an effective method of restore the peripheral nervous system injury due to its neuron-directed differentiation potential. This study was to evaluate the in vivo performance of BMSCs-packed Poly(3,4-ethylenedioxythiophene) (PEDOT) scaffolds across a critical nerve conduction gap and examine the potential mechanism by which BMSCs-packed PEDOT scaffolds mediate peripheral nerve regeneration in rat model of recurrent laryngeal nerve (RLN) deletion. In this study, rat models of 8 mm-RLN deletion were randomly divided into three groups and respectively received transplantation of autologous nerve or PEDOT scaffolds or BMSCspacked PEDOT scaffolds. After transplantation, neurological function was assessed by laryngeal electromyography. BMSCs-directed neuron differentiation was induced and confirmed by the upregulation of 6 nerve cell markers. Expression level of miRs, Notch signals and 6 nerve cell markers in nerve grafts or cells were determined by quantitative real-time PCR or western blot analysis. The results showed that BMSCs-packed PEDOT scaffolds transplantation significantly improved neurological function compared with PEDOT scaffolds alone. Regenerative nerve of BMSCs-packed PEDOT scaffolds showed higher expression level of miR-21 and Notch signals (Hes-1 and Notch intracellular domain (NICD)) than PEDOT alone. We found that the expression levels of miR-21, Hes-1 and NICD are increased with timecourse of neuron-directed differentiation stimulating of BMSCs. Meanwhile, both miR-21 overexpression and Notch pathway activation promote the expression of 6 nerve cell markers in BMSCs-directed neuron, whereas the inactivation of Notch pathway abrogates miR-21-inudced upregulation of 6 nerve cell markers. Moreover, knock-down of miR-21 suppresses the pro-neural restoration action of BMSCs-packed PEDOT scaffolds. In summary, our data suggested that BMSCs-packed PEDOT effectively repairs recurrent laryngeal nerve injury and the potential mechanism is miR-21- mediated Notch signal activation.
journal_name
Curr Neurovasc Resjournal_title
Current neurovascular researchauthors
Wu W,Zhang S,Chen Y,Liu Hdoi
10.2174/1567202614666161123112832subject
Has Abstractpub_date
2017-01-01 00:00:00pages
19-25issue
1eissn
1567-2026issn
1875-5739pii
CNR-EPUB-79898journal_volume
14pub_type
杂志文章abstract::Selective serotonin reuptake inhibitors (SSRIs) improve cognition in patients with stroke and increase the expression of brain-derived neurotrophic factor (BDNF) in the rat hippocampus. However, the effects of SSRIs on cognition and BDNF level in vascular dementia (VaD) patients are largely unknown. We performed an op...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:
更新日期:2013-08-19 00:00:00
abstract::Alzheimer's disease (AD) is characterized by extracellular beta-amyloid plaques and intracellular tau tangles. AD-related pathology is often accompanied by vascular changes. The predominant vascular lesions in AD are cerebral amyloid angiopathy (CAA) and arteriosclerosis. Platelets circulate along the vessel wall resp...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202612666150102124703
更新日期:2015-01-01 00:00:00
abstract::Activated platelets shed microparticles, which contain a variety of growth factors central to angiogenesis and neurogenesis. The aim of this study was to explore whether platelet derived microparticles (PMP) can boost endogenous neural stem cells dependent repair mechanisms following stroke in a rat model. To examine ...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720212801619018
更新日期:2012-08-01 00:00:00
abstract::Elevated alkaline phosphatase (ALP) levels are associated with cerebral small vascular diseases, such as silent brain infarction and cerebral white matter hyperintensity (cWMH), but few prospective data are available for cerebral microbleeds (CMBs). The aim of the study was to investigate associations between serum AL...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202613666160817095623
更新日期:2016-01-01 00:00:00
abstract::Oxaliplatin is a widely used chemotherapeutic agent that induces both acute and chronic peripheral neuropathy. Based on previous research indicating that estrogen replacement may attenuate some forms of pain in ovariectomized animals, we examined the effects of 17β-estradiol in OXAIPN. We discovered that local cold ex...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202613666160601144254
更新日期:2016-01-01 00:00:00
abstract::This review demonstrated that ischemic brain injury induces chronic changes in blood-brain barrier in the gray and white matter. This insufficiency of blood-brain barrier may allow entry of uncellular blood components such as different fragments of amyloid precursor protein and cellular blood components like leukocyte...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/156720207780637207
更新日期:2007-05-01 00:00:00
abstract::Stroke has been shown to lead to depressive disorders, anxiety disorders and other emotional consequences. Although the cause of these disorders is a subject of debate, stroke has clearly been shown to lead to the production of pro-inflammatory cytokines, which we hypothesized to play a role in the production of post-...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720209788970036
更新日期:2009-08-01 00:00:00
abstract::Serotonin (5-hydroxytryptamine, 5-HT) is an important neurotransmitter that modulates N-methyl-D-aspartate (NMDA) receptor activity by binding to several different 5-HT receptor subtypes. In the present study, we used whole-cell patch-clamp recordings in transverse slice preparations to test the role of 5-HT receptors...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202613666160823164136
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Iron homeostasis disorder and neuroinflammation are the most commonly known factors that promote the occurrence and development of cognitive impairment in people. Dexmedetomidine has an anti-inflammatory effect, and it reduces the incidence of postoperative cognitive dysfunction. Therefore, the aim of this s...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202617666200217105109
更新日期:2020-01-01 00:00:00
abstract::The use of cell-based therapy may be a valid therapeutic approach to ischemic brain injury. Stem cells have been proposed as a new form of cell based therapy in a variety of disorders, including acute and degenerative brain diseases. Up to date most efforts have concentrated on transplantation of embryonic stem cells ...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/1567202043361938
更新日期:2004-12-01 00:00:00
abstract::No longer considered exclusive for the function of the hematopoietic system, erythropoietin (EPO) is now considered as a viable agent to address central nervous system injury in a variety of cellular systems that involve neuronal, vascular, and inflammatory cells. Yet, it remains unclear whether the protective capacit...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720205774962683
更新日期:2005-12-01 00:00:00
abstract::Intracranial aneurysms (IAs) are acquired lesions in the brain and can pose potential risk of rupture leading to subarachnoid hemorrhage. Endoglin plays a pivotal role in the vascular development and disease. Variations of endoglin gene have been shown to be risk factors for IAs in different racial population. In the ...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202611666140912114450
更新日期:2014-01-01 00:00:00
abstract::Hematopoietic stem cells (HSCs) have long been defined as a cell with the capacity to repopulate the hematopoietic system of a lethally irradiated host. In clinical medicine, this property has been employed to reconstitute an individual's diseased hematopoietic system following ablation with a healthy, normal-function...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/1567202043362324
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Gait impairment after stroke is considered as a loss of cerebral function but is also the result of dysfunctional cerebral signals travelling to the spinal motor centres. A therapeutic option to restore disturbed cerebral network activity is deep brain stimulation (DBS). METHODS:A promising target for neuro...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202617666201201141046
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Endothelial Progenitor Cells (EPCs) have been suggested to be a therapeutic option in Acute Ischemic Stroke (AIS). Statins modulate endothelial function and preserve blood flow to tissue exposed to an ischemic insult. We tested the hypothesis that statins therapy might augment circulating EPCs in patients wi...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202615666180611120546
更新日期:2018-01-01 00:00:00
abstract::Cerebral aneurysm (CA) causes a life-threatening subarachnoid hemorrhage. However, no effective medical treatment to prevent the growth of CA is available. Nifedipine, a widely used calcium antagonist, was shown to improve endothelial function in various cardiovascular diseases. We examined whether nifedipine has a pr...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720208783565663
更新日期:2008-02-01 00:00:00
abstract::N-methyl-D-aspartate receptors (NMDA-Rs) activation has been implicated in various forms of synaptic plasticity depending on the receptor subtypes involved. However, the contribution of NR2A and NR2B subunits in glycine-induced long-term potentiation (LTP) of miniature excitatory postsynaptic currents (mEPSCs) in laye...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720213804805927
更新日期:2013-02-01 00:00:00
abstract::Increased homocysteine (Hcy) levels contribute to a variety of cardiovascular and cerebrovascular diseases including stroke and Alzheimer's disease. Recent data has shown that elevated levels of Hcy can lead to the blood-brain barrier (BBB) dysfunction and activation. However, the mechanism for Hcy-mediated dysfunctio...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720210792231787
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Lacunes and white matter hyperintensities (WMH) are two common findings seen on neuroimaging in patients with cerebral small vessel disease (cSVD). Clinically we observed that some patients with cSVD have aphasia through the language assessment scale. Our study aimed to explore the underlying risk factors fo...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202616666190227202638
更新日期:2019-01-01 00:00:00
abstract::This study was aimed to evaluate and compare the effect of chronic hypoxia and aging in the oxidative status of brain vessels and synaptosomes. For this purpose we isolated brain vessels and synaptosomes from 3- and 12-month-old rats subjected to chronic hypoxia (10% O₂ for 7 days) or normoxia (21% O₂). Several parame...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/156720210793180765
更新日期:2010-11-01 00:00:00
abstract::In multicellular organisms, apoptosis, also known as programmed cell death, is an important physiological response to eliminate unnecessary, excess, or harmful cells. Apoptosis occurs during embryonic development and is important in maintaining homeostasis during adulthood. Apoptosis also plays critical roles in angio...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/1567202043362072
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVE:Recently, a few studies have shown that non-traditional lipid parameters are associated with the hemorrhagic transformation (HT) and the clinical outcome of ischemic stroke. However, the role of non-traditional lipid parameters in ischemic stroke patients treated with intravenous thrombolysis remains unclear....
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202617999210101223129
更新日期:2021-01-01 00:00:00
abstract::Diabetes is associated with deficits in memory and cognitive functions and sustained inflammation. Recently, involvement of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) has been postulated in many cognitive functions, immune system and inflammation. Despite of role of NF-κB in inflammation, a...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202612666151030104810
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Dynamin-related protein 1 (DRP1) is a GTPase involved in mitochondrial fission, mitochondrial protein import, and drug sensitivity, suggesting an association with cancer progression. This study was conducted to evaluate the prognostic significance of DRP1 in glioblastoma multiforme (GBM). METHODS:DRP1 expre...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202617666200623123638
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:The most valid model for vascular cognitive impairment (VCI) is the mouse bilateral common carotid artery stenosis (BCAS), whose behavioral outcomes are supposedly affected by the remote ischemic conditioning (RIC) through the induction of autophgy. We hope to determine whether RIC contributes to the neuropr...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202614666170619081419
更新日期:2017-01-01 00:00:00
abstract::Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS). The acquired immune system plays a central role in the pathogenesis of MS although target antigens and effector mechanisms are still poorly defined. Studies in animal models of infectious or autoimmune encephalomyelitis suggest that the ...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/1567202043480152
更新日期:2004-04-01 00:00:00
abstract::Pericytes are perivascular cells and have heterogenous roles in the brain, such as controlling blood flow and entry of immune cells or regulating the blood-brain barrier. Platelet-derived growth factor (PDGF) receptor-beta (PDGFRβ) is highly expressed in pericytes, representing the most selective biomarker. The aim of...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202613666160219120411
更新日期:2016-01-01 00:00:00
abstract::Dendritic cells (DC) play a key role in determining the appropriate immune response to invading pathogens and tolerance to self antigens. Here I review the evidence that dendritic cell functions may be tuned by the sympathetic nervous system via the local release of norepinephrine. In the presence of antigens or micro...
journal_title:Current neurovascular research
pub_type: 杂志文章,评审
doi:10.2174/1567202053586776
更新日期:2005-04-01 00:00:00
abstract::Angiogenesis is associated with improved neurologic recovery after cerebral ischemia. Human bone marrow mesenchymal stem cells (hMSCs) have been successfully used to treat ischemic stroke and were shown to induce the expression of a number of neurotrophic factors including VEGF, epidermal growth factor (EGF) and basic...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/1567202611310020003
更新日期:2013-05-01 00:00:00
abstract::The blood-brain barrier (BBB) consists in part of a highly specialized set of cells which separates the brain from the vascular system. The BBB controls the entry and exit of substances from the brain tissue through tight junctions (TJs) between endothelial cells. It is known that the hormone prolactin (PRL) is able t...
journal_title:Current neurovascular research
pub_type: 杂志文章
doi:10.2174/15672026113109990025
更新日期:2013-11-01 00:00:00