Abstract：
:Growth characteristics and the expression of trophoblast-associated markers by six cell lines generated from midgestation chorioallantoic placentas of outbred (Holtzman) and inbred (Lewis, PVG.RT Ir8) rats were evaluated. The cells comprising all cell lines were epithelioid (contained cytokeratin-type intermediate filaments), had normal (2n, 4n) DNA content, and synthesized the extracellular matrix glycoprotein laminin. Variability was observed among the lines in all other characteristics: median cell size, rate of growth, serum dependency, responses to transferrin and dibutyryl cyclic adenosine-3',5'-monophosphate, synthesis of some major proteins, alkaline phosphatase activity, and the expression of immunoreactive placental lactogen-II. In general, cell lines with smaller mean cell sizes grew rapidly and required little serum for maintenance in vitro; cell lines with larger mean sizes grew more slowly and preferred higher concentrations of serum. Some associations between mean cell size/rate of growth and other characteristics were observed. No major differences were apparent between cell lines generated from outbred and inbred rat placentas. Trophoblast cell lines expressing distinct phenotypes provide a valuable new approach for studying a wide range of trophoblast cell activities.
journal_name
Placentajournal_title
Placentaauthors
Hunt JS,Deb S,Faria TN,Wheaton D,Soares MJdoi
10.1016/0143-4004(89)90038-6subject
Has Abstractpub_date
1989-03-01 00:00:00pages
161-77issue
2eissn
0143-4004issn
1532-3102pii
0143-4004(89)90038-6journal_volume
10pub_type
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