Abstract：
:Virus pseudotyping is a useful and safe technique for studying entry of emerging strains of influenza virus. However, few studies have compared different reassortant combinations in pseudoparticle systems, or compared entry kinetics of native viruses and their pseudotyped analogs. Here, vesicular stomatitis virus (VSV)-based pseudovirions displaying distinct influenza virus envelope proteins were tested for fusion activity. We produced VSV pseudotypes containing the prototypical X-31 (H3) HA, either alone or with strain-matched or mismatched N2 NAs. We performed single-particle fusion assays using total internal reflection fluorescence microscopy to compare hemifusion kinetics among these pairings. Results illustrate that matching pseudoparticles behaved very similarly to native virus. Pseudoparticles harboring mismatched HA-NA pairings fuse at significantly slower rates than native virus, and NA-lacking pseudoparticles exhibiting the slowest fusion rates. Relative viral membrane HA density of matching pseudoparticles was higher than in mismatching or NA-lacking pseudoparticles. An equivalent trend of HA expression level on cell membranes of HA/NA co-transfected cells was observed and intracellular trafficking of HA was affected by NA co-expression. Overall, we show that specific influenza HA-NA combinations can profoundly affect the critical role played by HA during entry, which may factor into viral fitness and the emergence of new pandemic influenza viruses.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hsu HL,Millet JK,Costello DA,Whittaker GR,Daniel Sdoi
10.1038/srep35537subject
Has Abstractpub_date
2016-10-18 00:00:00pages
35537issn
2045-2322pii
srep35537journal_volume
6pub_type
杂志文章abstract：:Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major rice disease. In Taiwan, the tropical indica type of Oryza sativa originally grown in this area is mix-cultivated with the temperate japonica type of O. sativa, and this might have led to adaptive changes of both rice host and Xoo isolates....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36575-x
更新日期：2019-01-22 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41598-018-19257-6
更新日期：2018-01-22 00:00:00
abstract：:The aim of this systematic review was taken to investigate the efficacy and safety of docetaxel plus thalidomide vs. docetaxel alone for treating androgen-independent prostate cancer (AIPC). Data were collected from different databases independently by three researchers according to the pre-defined inclusion and exclu...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep04818
更新日期：2014-04-28 00:00:00
abstract：:Understanding the factors that enable mutualisms to evolve and to subsequently remain stable over time, is essential to fully understand patterns of global biodiversity and for evidence based conservation policy. Theoretically, spatial heterogeneity of mutualists, through increased likelihood of fidelity between coope...
journal_title：Scientific reports
pub_type： 杂志文章,评审
doi：10.1038/srep14826
更新日期：2015-10-05 00:00:00
abstract：:Globally, there has been an increase in the use of herbal remedies including traditional Chinese medicine (TCM). There is a perception that products are natural, safe and effectively regulated, however, regulatory agencies are hampered by a lack of a toolkit to audit ingredient lists, adulterants and constituent activ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17475
更新日期：2015-12-10 00:00:00
abstract：:We aimed to explore the effects of type-2 diabetes mellitus (T2DM) and hypoglycemic therapy on the salivary microbiome in periodontitis patients and identify the potential salivary micro-biomarker for the early warning of T2DM. Saliva samples were collected from healthy individuals (Health), periodontitis patients (P)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72035-1
更新日期：2020-09-21 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-60629-8
更新日期：2020-02-25 00:00:00
abstract：:Two objects can be distinguished if they have different measurable properties. Thus, distinguishability depends on the Physics of the objects. In considering graphs, we revisit the Ising model as a framework to define physically meaningful spectral invariants. In this context, we introduce a family of refinements of t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05703
更新日期：2014-07-16 00:00:00
abstract：:When complementary resources are required for an optimal life cycle, most animals need to move between different habitats. However, the level of connectivity between resources can vary and, hence, influence individuals' behaviour. We show that landscape composition and configuration affect the connectivity between bre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21321-0
更新日期：2018-02-14 00:00:00
abstract：:Infantile hemangiomas are the most common infantile benign vascular tumor. While most infantile hemangiomas proliferate then involute, some may persist and require treatment for reasons including risk of disfigurement or functional impairment. Treatments currently include observation, pharmacological therapy, laser, c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21670
更新日期：2016-02-15 00:00:00
abstract：:Bragg coherent X-ray diffraction imaging (BCDI) has emerged as a powerful technique to image the local displacement field and strain in nanocrystals, in three dimensions with nanometric spatial resolution. However, BCDI relies on both dataset collection and phase retrieval algorithms that can induce artefacts in the r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53774-2
更新日期：2019-11-22 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-32881-6
更新日期：2018-10-12 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-28390-1
更新日期：2018-07-03 00:00:00
abstract：:Understanding the transcriptional regulatory elements that influence the progression of liver disease in the presence of hepatitis C virus (HCV) infection is critical for the development of diagnostic and therapeutic approaches. Systems biology provides a roadmap by which these elements may be integrated. In this stud...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32464-5
更新日期：2018-09-24 00:00:00
abstract：:Bismuth has long been a prototypical system for investigating phase transformations and melting at high pressure. Despite decades of experimental study, however, the lattice-level response of Bi to rapid (shock) compression and the relationship between structures occurring dynamically and those observed during slow (s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35260-3
更新日期：2018-11-16 00:00:00
abstract：:For historic individuals, the outward appearance and other phenotypic characteristics remain often non-resolved. Unfortunately, images or detailed written sources are only scarcely available in many cases. Attempts to study historic individuals with genetic data so far focused on hypervariable regions of mitochondrial...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20180-z
更新日期：2018-02-01 00:00:00
abstract：:The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon - consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli foll...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03053
更新日期：2013-10-25 00:00:00
abstract：:Functions of septin cytoskeletal polymers in tumorigenesis are still poorly defined. Their role in the regulation of cytokinesis and cell migration were proposed to contribute to cancer associated aneuploidy and metastasis. Overexpression of Septin 9 (Sept9) promotes migration of cancer cell lines. SEPT9 mRNA and prot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44976
更新日期：2017-03-24 00:00:00
abstract：:For lung and many other cancers, prognosis is essentially important, and extensive modeling has been carried out. Cancer is a genetic disease. In the past 2 decades, diverse molecular data (such as gene expressions and DNA mutations) have been analyzed in prognosis modeling. More recently, histopathological imaging da...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72201-5
更新日期：2020-09-14 00:00:00
abstract：:Overexposure to intense noise can destroy the synapses between auditory nerve fibers and their hair cell targets without destroying the hair cells themselves. In adult mice, this synaptopathy is immediate and largely irreversible, whereas, in guinea pigs, counts of immunostained synaptic puncta can recover with increa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76553-w
更新日期：2020-11-17 00:00:00
abstract：:The overall objective of this study was to test the hypothesis that +Gz (hypergravity/positive acceleration) and microgravity can both aggravate intervertebral disc degeneration (IVDD). Due to +Gz and microgravity, many pilots develop IVDD. However, the lack of animal models of IVDD under conditions of simulated +Gz a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53246-7
更新日期：2019-11-12 00:00:00
abstract：:Signaling by members of the transforming growth factor-β (TGF-β) superfamily, such as TGF-β3 and BMP7, and oxygen tension play a pivotal role in chondrocyte biology. The objective of this research was to investigate the endogenous BMP7 expression in human osteoarthritis (OA) cartilage and the effect of oxygen tension ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27602-y
更新日期：2018-07-06 00:00:00
abstract：:Fundamental cell signaling mechanisms that regulate dynamic remodeling of the extracellular matrix (ECM) in mechanically loaded tissues are not yet clearly understood. Trabecular meshwork (TM) tissue in the eye is under constant mechanical stress and continuous remodeling of ECM is crucial to maintain normal aqueous h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00845-x
更新日期：2017-04-11 00:00:00
abstract：:The expansion of agriculture is shrinking pristine forest areas worldwide, jeopardizing the persistence of their wild inhabitants. The Udzungwa red colobus monkey (Procolobus gordonorum) is among the most threatened primate species in Africa. Primarily arboreal and highly sensitive to hunting and habitat destruction, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14862
更新日期：2015-10-07 00:00:00
abstract：:This study was aimed at elucidating the changes of mandible symmetry in a rabbit model with low estrogen levels induced by ovariectomy (OVX) combined with temporomandibular joint (TMJ) anterior disc displacement without reduction (ADDWoR). 32 growing rabbits were randomly allocated into 4 groups; OVX group, ADDWoR gro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34023-4
更新日期：2018-10-23 00:00:00
abstract：:Identifying vital nodes in complex networks is a critical problem in the field of network theory. To this end, the Collective. Influence (CI) algorithm has been introduced and shows high efficiency and scalability in searching for the influential nodes in. the optimal percolation model. However, the crucial part of th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32874-5
更新日期：2018-09-28 00:00:00
abstract：:There is a growing interest in developing microphysiological systems that can be used to model both normal and pathological human organs in vitro. This "organs-on-chips" approach aims to capture key structural and physiological characteristics of the target tissue. Here we describe in vitro vascularized microtumors (V...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31589
更新日期：2016-08-23 00:00:00
abstract：:Glomerular mesangial cell (GMC) proliferation and matrix expansion are pathological hallmarks of a wide range of kidney diseases, including diabetic nephropathy. Although the circulating level of peptide hormone urotensin II (UII) and kidney tissue expression of UII and UII receptors (UTR) are increased in diabetic ne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18143-x
更新日期：2017-12-22 00:00:00
abstract：:Colaughter-simultaneous laughter between two or more individuals-allows listeners across different cultures and languages to quickly evaluate affiliation within a social group. We examined whether infants are sensitive to acoustic information in colaughter that indicates affiliation, specifically whether they can diff...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38954-4
更新日期：2019-03-11 00:00:00
abstract：:Continuous solidification experiments were carried out with Cu-Bi-Sn alloys under the effects of Electric Current Pulses (ECPs). A model describing the microstructure evolution was developed. The formation of the microstructure in the continuously solidified alloys was calculated. The calculations demonstrated that EC...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12680
更新日期：2015-07-31 00:00:00
