Abstract:
:Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image segmentation of cells in S. aureus cell clusters, and explicit identification of their cell division planes. We use a new combination of several existing analytical tools of image analysis to detect cellular and subcellular morphological features relevant to cell division from millisecond time scale sampled images of live pathogens at a detection precision of single molecules. We demonstrate this approach using a fluorescent reporter GFP fused to the protein EzrA that localises to a mid-cell plane during division and is involved in regulation of cell size and division. This image analysis framework presents a valuable platform from which to study candidate new antimicrobials which target the cell division machinery, but may also have more general application in detecting morphologically complex structures of fluorescently labelled proteins present in clusters of other types of cells.
journal_name
Phys Bioljournal_title
Physical biologyauthors
Wollman AJ,Miller H,Foster S,Leake MCdoi
10.1088/1478-3975/13/5/055002subject
Has Abstractpub_date
2016-10-17 00:00:00pages
055002issue
5eissn
1478-3967issn
1478-3975journal_volume
13pub_type
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