Abstract：
:R.PabI is a type II restriction enzyme that recognizes the 5'-GTAC-3' sequence and belongs to the HALFPIPE superfamily. Although most restriction enzymes cleave phosphodiester bonds at specific sites by hydrolysis, R.PabI flips the guanine and adenine bases of the recognition sequence out of the DNA helix and hydrolyzes the N-glycosidic bond of the flipped adenine in a similar manner to DNA glycosylases. In this study, we determined the structure of R.PabI in complex with double-stranded DNA without the R.PabI recognition sequence by X-ray crystallography. The 1.9 Å resolution structure of the complex showed that R.PabI forms a tetrameric structure to sandwich the double-stranded DNA and the tetrameric structure is stabilized by four salt bridges. DNA binding and DNA glycosylase assays of the R.PabI mutants showed that the residues that form the salt bridges (R70 and D71) are essential for R.PabI to find the recognition sequence from the sea of nonspecific sequences. R.PabI is predicted to utilize the tetrameric structure to bind nonspecific double-stranded DNA weakly and slide along it to find the recognition sequence.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wang D,Miyazono KI,Tanokura Mdoi
10.1038/srep35197subject
Has Abstractpub_date
2016-10-12 00:00:00pages
35197issn
2045-2322pii
srep35197journal_volume
6pub_type
杂志文章abstract：:Multi-junction (MJ) solar cells are one of the most promising technologies achieving high sunlight to electricity conversion efficiency. Resistive losses constitute one of the main underlying mechanisms limiting their efficiency under high illumination. In this paper, we study, by numerical modeling, the extent to whi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01854-6
更新日期：2017-05-11 00:00:00
abstract：:Laser heating of gold nanospheres (GNS) is increasingly prevalent in biomedical applications due to tunable optical properties that determine heating efficiency. Although many geometric parameters (i.e. size, morphology) can affect optical properties of individual GNS and their heating, no specific studies of how GNS ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79393-w
更新日期：2021-01-13 00:00:00
abstract：:The black carbon or elemental carbon (EC) content in ice and snow has been a concern in climate change studies, but time-series records have mostly been obtained from glacier ice-core samples in limited geographical locations, such as the Arctic or high mountains. This is the first study to present decade-long records...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61067-2
更新日期：2020-03-18 00:00:00
abstract：:Increased activity of brown adipose tissue (BAT) activity in adults is thought to prevent obesity. Therefore, regulators of BAT activity might serve as anti-obesity therapy in future, but are not investigated thoroughly up to now. In our study, we assessed retrospectively the association of BAT activity with several e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37924-6
更新日期：2019-02-13 00:00:00
abstract：:Urothelial carcinoma (UC) is a common disease causing significant morbidity and mortality as well as considerable costs for health systems. Extensive aberrant methylation of DNA is broadly documented in early UC, contributing to genetic instability, altered gene expression and tumor progression. However the triggers i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60796-8
更新日期：2020-03-02 00:00:00
abstract：:In the Ultimatum Game, two players are offered a chance to win a pie. The proposer suggests how to split the pie. The responder can either accept or reject the deal. If an agreement is not reached, neither player gets anything. Both game theory and evolutionary game theory predict the rational solution that the propos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04534
更新日期：2014-04-01 00:00:00
abstract：:Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111In-labelled DOTA-conjugated ZEGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expressing xenografts in mice. A...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05700-7
更新日期：2017-07-20 00:00:00
abstract：:The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in position 2 of the stem peptide plays a decisive role in the polymerization of cell wall building blocks. S. aureus mutants with a reduced degree of amidation are less viable and show increased susceptibility to methicil...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31098-x
更新日期：2018-08-28 00:00:00
abstract：:Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed germination are la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22073
更新日期：2016-02-23 00:00:00
abstract：:We study the dynamical Casimir-Polder force on a two-level atom with different initial states in the one-dimensional dielectric cavity with output coupling, and obtain the analytical expression of the expectation value of dynamical Casimir-Polder force. Results show that the expectation values of dynamical Casimir-Pol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68546-6
更新日期：2020-07-20 00:00:00
abstract：:Ricco's area (the largest area of visual space in which stimulus area and intensity are inversely proportional at threshold) has previously been hypothesised to be a result of centre/surround antagonism in retinal ganglion cell receptive fields, but recent evidence suggests a sizeable cortical contribution. Here, Ricc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21620-6
更新日期：2018-03-01 00:00:00
abstract：:Propolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC-MS), B. monnieri extracts (BcH, B...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78014-w
更新日期：2020-12-03 00:00:00
abstract：:Once assumed only to be a waste product of anaerobe glycolytic activity, lactate is now recognized as an energy source in skeletal muscles. While lactate metabolism has been extensively studied in vivo, underlying cellular processes are poorly described. This study aimed to examine lactate metabolism in cultured human...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28249-5
更新日期：2018-06-29 00:00:00
abstract：:Small oligomers formed early along human islet amyloid polypeptide (hIAPP) aggregation is responsible for the cell death in Type II diabetes. The epigallocatechin gallate (EGCG), a green tea extract, was found to inhibit hIAPP fibrillation. However, the inhibition mechanism and the conformational distribution of the s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33076
更新日期：2016-09-13 00:00:00
abstract：:Although acute psychosocial stress can impact autobiographical memory retrieval, the nature of this effect is not entirely clear. One reason for this ambiguity is because stress can have opposing effects on the different stages of autobiographical memory retrieval. We addressed this issue by testing how acute stress a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26890-8
更新日期：2018-06-06 00:00:00
abstract：:Transjugular intrahepatic portosystemic shunt (TIPS) can treat portal hypertensive complications and modifies hepatic hemodynamics. Modification of liver perfusion can alter contrast enhancement dynamics of liver nodules. This study investigated the diagnostic performance of contrast-enhanced ultrasound (CEUS) to diag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77801-9
更新日期：2020-11-26 00:00:00
abstract：:Changes in the stability, hydrogen diffusion, and mechanical properties of the NbH phases from Ni-doping was studied by using first-principles methods. The calculation results reveal that the single H atom adsorption is energetically favorable at the tetrahedral interstitial site (TIS) and octahedral interstitial site...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06658-2
更新日期：2017-07-26 00:00:00
abstract：:Bacterial cells have characteristic spatial and temporal scales. For instance, Escherichia coli, the typical rod-shaped bacteria, always maintains a relatively constant cell width and cell division time. However, whether the external physical perturbation of cell width has an impact on cell division time remains large...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74778-3
更新日期：2020-10-20 00:00:00
abstract：:The association between cancer and volatile organic metabolites in exhaled breaths has attracted increasing attention from researchers. The present study reports on a systematic study of gas profiles of metabolites in human exhaled breath by pattern recognition methods. Exhaled breath was collected from 85 patients wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05383
更新日期：2014-06-20 00:00:00
abstract：:The total spatial coverage of Marine Protected Areas (MPAs) within the Brazilian Economic Exclusive Zone (EEZ) has recently achieved the quantitative requirement of the Aichii Biodiversity Target 11. However, the distribution of MPAs in the Brazilian EEZ is still unbalanced regarding the proportion of protected ecosys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45042-0
更新日期：2019-06-14 00:00:00
abstract：:Oxaliplatin, a chemotherapy medication, causes severe peripheral neuropathy. Although oxaliplatin-induced peripheral neuropathy is a dose-limiting toxicity, a therapeutic strategy against its effects has not been established. We previously reported the involvement of N-methyl-D-aspartate receptors and their intracellu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08891-1
更新日期：2017-08-29 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-32119-5
更新日期：2018-09-11 00:00:00
abstract：:The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNAPyl are extensively used to add non-canonical amino acids (ncAAs) to the genetic code of bacterial and eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process to generate an appropriate PylRS/tRNAPyl pair. We here report a s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48357-0
更新日期：2019-08-19 00:00:00
abstract：:Printing techniques are becoming increasingly prevalent in modern manufacturing. However, its biggest drawback is the limit in printing resolution. In this paper, we present solvodynamic printing as a novel printing system which aims to improve print resolution by incorporating an additional immiscible carrier solvent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47105-8
更新日期：2019-07-24 00:00:00
abstract：:A culture system that can recapitulate myelination in vitro will not only help us better understand the mechanism of myelination and demyelination, but also find out possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible myelination culture system using mou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15122
更新日期：2015-10-12 00:00:00
abstract：:State of charge (SOC) is a crucial index used in the assessment of electric vehicle (EV) battery storage systems. Thus, SOC estimation of lithium-ion batteries has been widely investigated because of their fast charging, long-life cycle, and high energy density characteristics. However, precise SOC assessment of lithi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61464-7
更新日期：2020-03-13 00:00:00
abstract：:Cell-free enzymatic reaction cascades combine the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. The absence of a regulatory cell environment enables direct process control including methods for facile bottleneck identification and process optimization. Within this ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48151-y
更新日期：2019-08-13 00:00:00
abstract：:Holography--originally developed for correcting spherical aberration in transmission electron microscopes--is now used in a wide range of disciplines that involve the propagation of waves, including light optics, electron microscopy, acoustics and seismology. In electron microscopy, the two primary modes of holography...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07020
更新日期：2014-11-12 00:00:00
abstract：:The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07760
更新日期：2015-01-14 00:00:00
abstract：:For translocated animals, behavioral competence may be key to post-release survival. However, monitoring behavior is typically limited to tracking movements or inferring behavior at a gross scale via collar-mounted sensors. Animal-bourne acoustic monitoring may provide a unique opportunity to monitor behavior at a fin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41455-z
更新日期：2019-03-26 00:00:00
