Abstract：
:Although re-sequencing of gene panels and mRNA expression profiling are now firmly established in clinical laboratories, in-depth proteome analysis has remained a niche technology, better suited for studying model systems rather than challenging materials such as clinical trial samples. To address this limitation, we have developed a novel and optimized platform called SP3-Clinical Tissue Proteomics (SP3-CTP) for in-depth proteome profiling of practical quantities of tumour tissues, including formalin fixed and paraffin embedded (FFPE). Using single 10 μm scrolls of clinical tumour blocks, we performed in-depth quantitative analyses of individual sections from ovarian tumours covering the high-grade serous, clear cell, and endometrioid histotypes. This examination enabled the generation of a novel high-resolution proteome map of ovarian cancer histotypes from clinical tissues. Comparison of the obtained proteome data with large-scale genome and transcriptome analyses validated the observed proteome biology for previously validated hallmarks of this disease, and also identified novel protein features. A tissue microarray analysis validated cystathionine gamma-lyase (CTH) as a novel clear cell carcinoma feature with potential clinical relevance. In addition to providing a milestone in the understanding of ovarian cancer biology, these results show that in-depth proteomic analysis of clinically annotated FFPE materials can be effectively used as a biomarker discovery tool and perhaps ultimately as a diagnostic approach.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hughes CS,McConechy MK,Cochrane DR,Nazeran T,Karnezis AN,Huntsman DG,Morin GBdoi
10.1038/srep34949subject
Has Abstractpub_date
2016-10-07 00:00:00pages
34949issn
2045-2322pii
srep34949journal_volume
6pub_type
杂志文章abstract：:Breast tissue heterogeneity is related to risk factors that lead to more aggressive tumour growth and worse prognosis, yet such heterogeneity has not been well characterized. The aim of this study is to reveal the heterogeneous signal patterns of the apparent diffusion coefficient (ADC) of a tumour and its surrounding...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03122-z
更新日期：2017-06-06 00:00:00
abstract：:Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water tha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52445-6
更新日期：2019-11-06 00:00:00
abstract：:Botulinum neurotoxins (BoNTs) are highly potent toxins that cleave neuronal SNARE proteins required for neurotransmission, causing flaccid paralysis and death by asphyxiation. Currently, there are no clinical treatments to delay or reverse BoNT-induced blockade of neuromuscular transmission. While aminopyridines have ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16064-3
更新日期：2017-11-20 00:00:00
abstract：:The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) to reach their full potential in the treatment of chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel was developed to deliver BMSCs, which improve the chronic inflammation microenvironments ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18104
更新日期：2015-12-08 00:00:00
abstract：:The rapid urbanization in China has been associated with a growing hunger for energy consumption and steadily-increasing CO2 emissions. In this paper, an integrated system dynamics model composed of four sub-models is developed to simulate the urbanization and energy consumption in China from 1998 to 2050. Three scena...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66125-3
更新日期：2020-06-19 00:00:00
abstract：:Traditionally, the practice of the tibial component placement in total knee arthroplasty has focused on achieving maximum coverage without malrotation. However, the concept of maximizing coverage has not been well defined or researched and yet biased results are often produced. This study aimed to evaluate the effect ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67613-2
更新日期：2020-06-29 00:00:00
abstract：:We design a soft infrared metamaterial absorber based on gold nanorods dispersed in liquid crystal (LC) placed on a gold film and theoretically investigate its total absorption character. Because the nanorods align with the LC molecule, the gold nanorods/LC hybrid exhibits different permittivity as a function of tilt ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16698
更新日期：2015-11-18 00:00:00
abstract：:Glucocorticoids (GC) display pleiotropic effects on the immune system. Macrophages are a major target for GC action. Here we show that dexamethasone (DEX), a synthetic GC, decreased viability of naïve bone marrow-derived macrophages (BMDM), involving an apoptotic mechanism. Administration of DEX together with lipopoly...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04323
更新日期：2014-03-10 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-44071-z
更新日期：2019-06-05 00:00:00
abstract：:The ratio of ω-6 to ω-3 polyunsaturated fatty acids (PUFAs) appears to be critical in the regulation of various pathophysiological processes and to maintain cellular homeostasis. While a high proportion of dietary intake of ω-6 PUFAs is associated with various inflammatory disorders, higher intake of ω-3 PUFAs is know...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31649
更新日期：2016-08-18 00:00:00
abstract：:Previous studies showed that better adherence to the Mediterranean diet (MD) is associated with lower risk of chronic diseases, but limited data are available on bone health. We investigated the association of the MD with bone mineral density (BMD) in Chinese adults. We included 2371 participants aged 40-75 years in t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25662
更新日期：2016-05-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10037-2
更新日期：2017-11-08 00:00:00
abstract：:A fast Endospore Germinability Assay (EGA) was validated with traditional plate counts to enumerate single endospore germination events for monitoring surface sterilization. The assay is based on a time-gated luminescence microscopy technique enabling visualization and enumeration of individual germinating endospores....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57175-3
更新日期：2020-01-16 00:00:00
abstract：:The specification and growth of organs is controlled simultaneously by networks of transcription factors. While the connection between these transcription factors with fate determinants is increasingly clear, how they establish the link with the cell cycle is far less understood. Here we investigate this link in the d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04936-7
更新日期：2017-07-07 00:00:00
abstract：:n-3 polyunsaturated fatty acids (PUFAs) and n-6 PUFAs are reported to have immunomodulatory effects, but few studies have examined these functions. Thus, we examined whether dietary n-3 and n-6 PUFAs are associated with the risk of gastric cancer and further investigated whether fatty acid desaturases 1 and 2 (FADS1 a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21960-3
更新日期：2018-02-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35405
更新日期：2016-10-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56947-1
更新日期：2020-01-15 00:00:00
abstract：:Here we describe a unique microbial biotechnology for simultaneous bioremediation and biomining of twelve ionic metals overcoming the obstacles of multimetal toxicity to microbes. After a thorough search of key microorganisms in microbiomes of many sulfidic springs in Bavaria (Germany) over an area of 200 km2, we foun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46560-7
更新日期：2019-07-16 00:00:00
abstract：:The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44320
更新日期：2017-03-13 00:00:00
abstract：:Alcohol use disorder (AUD) is a chronic and relapsing disease with a substantial genetic influence. Given the recent discovery of the association of PPM1G methylation with alcohol use disorder (AUD) from a genome-wide methylation study, we sought to verify and extend the previous work of AUD-related impulsivity in a K...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62504-y
更新日期：2020-03-26 00:00:00
abstract：:Up to now, most of stimulated-emission-depletion (STED) systems were lens-based bulky systems. Exchanging some spatial light paths with optical fiber components will make the systems more flexible and will benefit various fields. A big problem to achieve this goal is that the STED beam generated by the traditional met...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44905-w
更新日期：2019-06-11 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is a common and severe X-linked myopathy, characterized by muscle degeneration due to altered or absent dystrophin. DMD has no effective cure, and the underlying molecular mechanisms remain incompletely understood. The aim of this study is to investigate the metabolic changes in DMD u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81090-1
更新日期：2021-01-21 00:00:00
abstract：:In typical numerical simulations, the space-charge force is calculated by slicing a beam into many longitudinal segments and by solving the two-dimensional Poisson equation in each segment. This method neglects longitudinal leakage of the space-charge force to nearby segments owing to its longitudinal spread over 1/γ....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30960-2
更新日期：2018-08-24 00:00:00
abstract：:The triatomine Rhodnius prolixus, a vector of the etiological agent of Chagas disease, has long been used as model to understand important aspects of insect physiology. Despite this history, the impact of the nutritional state on regulatory pathways associated with reproductive performance in triatomines has never bee...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67932-4
更新日期：2020-07-10 00:00:00
abstract：:The association between perioperative blood transfusion (PBT) with adverse oncological outcomes have been previously reported in multiple malignancies including RCC. Nevertheless, the importance of transfusion timing is still unclear. The primary purpose of this study is to appraise whether the receipt of intraoperati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37691-4
更新日期：2019-02-04 00:00:00
abstract：:Oxidative stress due to hypobaric hypoxia at extreme altitudes causes severe neuronal damage and irreversible cognitive loss. Owing to contraindications of current drug therapies, the aim of the study was to investigate memory enhancing potential of aqueous extract of Ganoderma lucidum (GLAQ) and underlying neuroprote...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65812-5
更新日期：2020-06-02 00:00:00
abstract：:The prediction of infarction volume after stroke onset depends on the shape of the growth dynamics of the infarction. To understand growth patterns that predict lesion volume changes, we studied currently available models described in literature and compared the models with Adaptive Neuro-Fuzzy Inference System [ANFIS...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08044-4
更新日期：2017-08-08 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep16188. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/srep46909
更新日期：2017-12-22 00:00:00
abstract：:There is increasing interest in the development of noninvasive diagnostic methods for early cancer detection, to improve the survival rate and quality of life of cancer patients. Identification of volatile metabolic compounds may provide an approach for noninvasive early diagnosis of malignant diseases. Here we analyz...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05196
更新日期：2014-06-06 00:00:00
abstract：:Schistosomes, unlike malaria parasites, are in their diploid stage when targeted by the human immune system. Diploids can be either homozygous or heterozygous. The difference has profound significance for developing immunity and yet has not previously been addressed. We examined the implications of zygosity on immunit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00346
更新日期：2012-01-01 00:00:00
