Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy.

Abstract:

:Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.

journal_name

Nat Microbiol

journal_title

Nature microbiology

authors

Wakisaka Y,Suzuki Y,Iwata O,Nakashima A,Ito T,Hirose M,Domon R,Sugawara M,Tsumura N,Watarai H,Shimobaba T,Suzuki K,Goda K,Ozeki Y

doi

10.1038/nmicrobiol.2016.124

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

16124

issue

10

issn

2058-5276

pii

nmicrobiol2016124

journal_volume

1

pub_type

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