Abstract:
:The local flexibility of an enzyme's active center plays pivotal roles in catalysis, however, little is known about how the flexibility of these flexible residues affects stability. In this study, we proposed an active center stabilization (ACS) strategy to improve the kinetic thermostability of Candida rugosa lipase1. Based on the B-factor ranking at the region ~10 Å within the catalytic Ser209, 18 residues were selected for site-saturation mutagenesis. Based on three-tier high-throughput screening and ordered recombination mutagenesis, the mutant VarB3 (F344I/F434Y/F133Y/F121Y) was shown to be the most stable, with a 40-fold longer in half-life at 60 °C and a 12.7 °C higher Tm value than that of the wild type, without a decrease in catalytic activity. Further analysis of enzymes with different structural complexities revealed that focusing mutations on the flexible residues within around 10 Å of the catalytic residue might increase the success rate for enzyme stabilization. In summary, this study identifies a panel of flexible residues within the active center that affect enzyme stability. This finding not only provides clues regarding the molecular evolution of enzyme stability but also indicates that ACS is a general and efficient strategy for exploring the functional robustness of enzymes for industrial applications.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhang XF,Yang GY,Zhang Y,Xie Y,Withers SG,Feng Ydoi
10.1038/srep33797subject
Has Abstractpub_date
2016-09-26 00:00:00pages
33797issn
2045-2322pii
srep33797journal_volume
6pub_type
杂志文章abstract::Neutrophil elastase (NE) suppresses IL-8/CXCL8 in human airway smooth muscle cells (hASM) while stimulating its production in respiratory epithelial cells. This differential effect is mediated by the selective induction of NKRF and dysregulation in chronic inflammatory diseases. We hypothesized that the differential a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44930
更新日期:2017-03-21 00:00:00
abstract::Botulinum-toxin A (BoNT/A) is used for a wide range of conditions. Intramuscular administration of BoNT/A inhibits the release of acetylcholine at the neuromuscular junction from presynaptic motor neurons causing muscle-paralysis. The aim of the present study was to investigate the effect of high dose intramuscular Bo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14997-3
更新日期:2017-11-07 00:00:00
abstract::In contrast to mild infections of Group A Streptococcus (GAS) invasive infections of GAS still pose a serious health hazard: GAS disseminates from sterile sites into the blood stream or deep tissues and causes sepsis or necrotizing fasciitis. In this case antibiotics do not provide an effective cure as the bacteria ar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11276-z
更新日期:2017-09-18 00:00:00
abstract::Plant diseases are a major cause for yield losses and new strategies to control them without harming the environment are urgently needed. Plant-associated bacteria contribute to their host's health in diverse ways, among which the emission of disease-inhibiting volatile organic compounds (VOCs). We have previously rep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55218-3
更新日期:2019-12-30 00:00:00
abstract::Since the discovery of nuclear gamma-rays, its imaging has been limited to pseudo imaging, such as Compton Camera (CC) and coded mask. Pseudo imaging does not keep physical information (intensity, or brightness in Optics) along a ray, and thus is capable of no more than qualitative imaging of bright objects. To attain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41511
更新日期:2017-02-03 00:00:00
abstract::Activating transcription factor 4 (ATF4) is constitutively expressed in a variety of tissues, and regulates several pathological features associated with metabolic diseases such as non-alcoholic fatty liver diseases (NAFLD) and obesity. However, the role of ATF4 in animal model systems is poorly understood. To investi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16587-9
更新日期:2017-11-27 00:00:00
abstract::Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59988-z
更新日期:2020-02-20 00:00:00
abstract::The alpine meadow on the Tibetan Plateau is the highest and largest pasture in the world, and its formation and distribution are mainly controlled by Indian summer monsoon effects. However, little is known about how monsoon-related cues may trigger spring phenology of the vast alpine vegetation. Based on the 7-year ob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20985
更新日期:2016-02-09 00:00:00
abstract::Bovine TB (bTB) is endemic in Irish cattle and has eluded eradication despite considerable expenditure, amid debate over the relative roles of badgers and cattle in disease transmission. Using a comprehensive dataset from Northern Ireland (>10,000 km(2); 29,513 cattle herds), we investigated interactions between host ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13062
更新日期:2015-08-17 00:00:00
abstract::The design, fabrication, and characterization of ultra-high responsivity photodetectors based on mesoscopic multilayer MoS2 is presented, which is a less explored system compared to direct band gap monolayer MoS2 that has received increasing attention in recent years. The device architecture is comprised of a metal-se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19367-1
更新日期:2018-01-19 00:00:00
abstract::CSNK2B, which encodes the beta subunit of casein kinase II (CK2), plays an important role in neuron morphology and synaptic transmission. Variants in CSNK2B associated with epilepsy and/or intellectual disability (ID)/developmental delay (DD) have been reported in five cases only. Among the 816 probands suspected here...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53484-9
更新日期:2019-11-29 00:00:00
abstract::In the jejunum, PepT1 is particularly enriched in the well-differentiated absorptive epithelial cells in the villi. Studies of expression and function of PepT1 along the crypt-villus axis demonstrated that this protein is crucial to the process of di/tripeptide absorption. We recently exhibited that PepT1 plays an imp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27119
更新日期:2016-06-01 00:00:00
abstract::We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24622-6
更新日期:2018-04-27 00:00:00
abstract::Whipple's disease is a systemic infectious disease associated with the bacterium Tropheryma whipplei. Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23883
更新日期:2016-03-30 00:00:00
abstract::Studies reveal that there exists much interaction and communication between bacterial cells, with parts of these social behaviors depending on cell-cell contacts. The cell-cell contact has proved to be crucial for determining various biochemical processes. However, for cell culture with relatively low cell concentrati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01993
更新日期:2013-01-01 00:00:00
abstract::Familial breast cancer is estimated to account for 15-20% of all cases of breast cancer. Surveillance for familial breast cancer is well-established world-wide. However, this service does not exist in Jordan, due to the scarcity of information with regard to the genetic profiling of these patients, and therefore lack ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74250-2
更新日期:2020-10-16 00:00:00
abstract::Maternal obesity in pregnancy increases the risk of adverse long-term health outcomes in both mother and offspring. A population-based cohort of prospectively collected routine antenatal healthcare data collected between January 2003 and September 2017 at University Hospital Southampton, UK was utilised to investigate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45595-0
更新日期:2019-06-24 00:00:00
abstract::Plant cell cultures are an attractive platform for the production of recombinant proteins. A major drawback, hindering the establishment of plant cell suspensions as an industrial platform, is the low product yield obtained thus far. Histone acetylation is associated with increased transcription levels, therefore it i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17006-9
更新日期:2017-12-01 00:00:00
abstract::Animal societies exhibit remarkable variation in their breeding strategies. Individuals can maximize their fitness by either reproducing or by helping relatives. Social hymenopterans have been key taxa for the study of Hamilton's inclusive fitness theory because the haplodiploid sex-determination system results in asy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26635
更新日期:2016-05-23 00:00:00
abstract::We aimed to investigate the efficacy and safety of primary retropupillary iris claw intraocular lens (R-IOL) implantation in patients with complete intraocular lens (IOL) dislocation. In this single-center retrospective case series, we reviewed the medical records of patients who underwent R-IOL implantation surgery w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80292-3
更新日期:2021-01-12 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is an EBV-associated epithelial malignancy prevalent in southern China. Presence of treatment-resistant cancer stem cells (CSC) may associate with tumor relapse and metastasis in NPC. ICG-001 is a specific CBP/β-catenin antagonist that can block CBP/β-catenin-mediated transcription of st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09979
更新日期:2015-04-21 00:00:00
abstract::Head and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38797-z
更新日期:2019-02-19 00:00:00
abstract::Microtubule-associated protein 1B (MAP1B) is expressed predominantly during the early stages of development of the nervous system, where it regulates processes such as axonal guidance and elongation. Nevertheless, MAP1B expression in the brain persists in adult stages, where it participates in the regulation of the st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30069
更新日期:2016-07-18 00:00:00
abstract::Power induced wave collapse is one of the most fascinating phenomena in optics as it provides extremely high intensities, thus stimulating a range of nonlinear processes. For low power levels, propagation of beams in bulk media is dominated by diffraction, while above a certain threshold self-focusing is steadily enha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46060-8
更新日期:2019-07-02 00:00:00
abstract::The present study evaluates the potential of Sar gassum muticum (Sar) and Jan ia rubens (Jan) seaweeds for enhancing growth and mitigating soil-salinity in chickpea (Cicer arietinum L.). Under control conditions, Sar and Jan extracts improved chickpea growth which was attributed to their potential for increasing photo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07692-w
更新日期:2017-09-05 00:00:00
abstract::Plant resistance can provide effective, economical, and sustainable pest control. Tolerance to the soybean aphid has been identified and confirmed in the soybean KS4202. Although its resistance mechanisms are not fully understood, evidence suggests that enhanced detoxification of reactive oxygen species (ROS) is an ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33477-w
更新日期:2018-10-11 00:00:00
abstract::The villous cytotrophoblastic cells have the ability to fuse and differentiate, forming the syncytiotrophoblast (STB). The syncytialisation process is essential for placentation. Nevertheless, the mechanisms involved in cell fusion and differentiation are yet to be fully elucidated. It has been suggested that cell sur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66554-0
更新日期:2020-06-15 00:00:00
abstract::Skeletal muscle represents the largest pool of body zinc, however, little is known about muscle zinc homeostasis or muscle-specific zinc functions. Zip14 (Slc39a14) was the most highly expressed zinc transporter in skeletal muscle of mice in response to LPS-induced inflammation. We compared metabolic parameters of ske...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61059-2
更新日期:2020-03-04 00:00:00
abstract::Human behaviors exhibit ubiquitous correlations in many aspects, such as individual and collective levels, temporal and spatial dimensions, content, social and geographical layers. With rich Internet data of online behaviors becoming available, it attracts academic interests to explore human mobility similarity from t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12274-x
更新日期:2017-09-20 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-67554-w
更新日期:2020-06-25 00:00:00