Abstract：
:Clonal cell analysis defines the potential of single cells and the diversity they can produce. To achieve this, we have developed a novel adaptation of the genetic tracing strategy, UbC-StarTrack, which attributes a specific and unique color-code to single neural precursors, allowing all their progeny to be tracked. We used integrable fluorescent reporters driven by a ubiquitous promoter in PiggyBac-based vectors to achieve inheritable and stable clonal cell labeling. In addition, coupling this to an inducible Cre-LoxP system avoids the expression of non-integrated reporters. To assess the utility of this system, we first analyzed images of combinatorial expression of fluorescent reporters in transfected cells and their progeny. We also validated the efficiency of the UbC-StarTrack to trace cell lineages through in vivo, in vitro and ex vivo strategies. Finally, progenitors located in the lateral ventricles were targeted at embryonic or postnatal stages to determine the diversity of neurons and glia they produce, and their clonal relationships. In this way we demonstrate that UbC-StarTrack can be used to identify all the progeny of a single cell and that it can be employed in a wide range of contexts.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Figueres-Oñate M,García-Marqués J,López-Mascaraque Ldoi
10.1038/srep33896subject
Has Abstractpub_date
2016-09-22 00:00:00pages
33896issn
2045-2322pii
srep33896journal_volume
6pub_type
杂志文章abstract：:Sea ice is an important component of the global climate system and a key indicator of climate change. A decreasing trend in Arctic sea-ice concentration is evident in recent years, whereas Antarctic sea-ice concentration exhibits a generally increasing trend. Various studies have investigated the underlying causes of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45804
更新日期：2017-04-05 00:00:00
abstract：:Nitric oxide (NO), a vascular signaling molecule, is primarily produced by endothelial NO synthase. Recently, a functional endothelial NO synthase (eNOS) was described in red blood cells (RBC). The RBC-eNOS contributes to the intravascular NO pool and regulates physiological functions. However the regulatory mechanism...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26935
更新日期：2016-06-27 00:00:00
abstract：:The National Lung Screening Trial (NLST) demonstrated that screening with low-dose computed tomography (LDCT) is associated with a 20% reduction in lung cancer mortality. One potential limitation of LDCT screening is overdiagnosis of slow growing and indolent cancers. In this study, peritumoral and intratumoral radiom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67378-8
更新日期：2020-06-29 00:00:00
abstract：:The Alzheimer's disease pathology is associated with accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. The formation of initial nucleus triggers conformational changes in Tau and leads to its deposition. Hence, there is a need to eliminate these toxic proteins for proper functioni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60773-1
更新日期：2020-03-04 00:00:00
abstract：:The TCP transcription factors usually act as integrators of multiple growth regulatory and environmental stimuli. However, little is known about this gene family in the important tropical crop cassava (Manihot esculenta). In this study, 36 TCP genes were identified and renamed based on cassava whole-genome sequence an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09398-5
更新日期：2017-08-30 00:00:00
abstract：:Tumorigenesis is driven by genetic alterations that perturb the signaling networks regulating proliferation or cell death. In order to block tumor growth, one has to precisely know how these signaling pathways function and interplay. Here, we identified the transcription factor Ets21C as a pivotal regulator of tumor g...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34725
更新日期：2016-10-07 00:00:00
abstract：:This article puts forward a technique for extracting the density of trap states (DOST) distribution based on the transient photo-voltage (TPV) measurement result. We prove that when the TPV result is linear, the DOST distribution is exponential type and vice versa. Compared to the approach based on the space charge li...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69914-y
更新日期：2020-07-30 00:00:00
abstract：:Previous studies have evaluated gene expression in Alzheimer's disease (AD) brains to identify mechanistic processes, but have been limited by the size of the datasets studied. Here we have implemented a novel meta-analysis approach to identify differentially expressed genes (DEGs) in published datasets comprising 450...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep12393
更新日期：2015-07-23 00:00:00
abstract：:Millions of people are blind worldwide. Sensory substitution (SS) devices (e.g., vOICe) can assist the blind by encoding a video stream into a sound pattern, recruiting visual brain areas for auditory analysis via crossmodal interactions and plasticity. SS devices often require extensive training to attain limited fun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15628
更新日期：2015-10-22 00:00:00
abstract：:Angiogenesis is one of the hallmarks of cancer and, as such, one of the alternative general targets for anticancer therapy. Since angiogenesis is a complex process involving a high number of interconnected components, a network approach would be a convenient systemic way to analyse responses to directed drug attacks. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00061
更新日期：2011-01-01 00:00:00
abstract：:Outbreaks of multidrug resistant bacteria including vancomycin-resistant enterococci (VRE) in healthcare institutions are increasing in Norway, despite a low level of resistance compared to other European countries. In this study, we describe epidemiological relatedness of vancomycin-resistant Enterococcus faecium iso...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48436-2
更新日期：2019-08-15 00:00:00
abstract：:RBM20 is a major regulator of heart-specific alternative pre-mRNA splicing of TTN encoding a giant sarcomeric protein titin. Mutation in RBM20 is linked to autosomal-dominant familial dilated cardiomyopathy (DCM), yet most of the RBM20 missense mutations in familial and sporadic cases were mapped to an RSRSP stretch i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26624-w
更新日期：2018-06-12 00:00:00
abstract：:Novel-morphological Fe3O4 nanosheets with magnetochromatic property have been prepared by a modified solvothermal method. Such nanosheets could form one-dimension photonic crystal under an external magnetic field. The Fe3O4 nanosheets suspension could strongly diffract visible light and display varied colors with chan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09320
更新日期：2015-03-23 00:00:00
abstract：:Cetacean strandings (CS) have been reported in increasing numbers in coastal areas worldwide. Although the causes of these strandings are unknown, a number of anthropogenic and environmental factors have been suggested. This paper aims to characterize CS patterns and describe their fine-scale spatiotemporal dynamics. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66484-x
更新日期：2020-06-11 00:00:00
abstract：:The niacin receptor HCA2 is implicated in controlling inflammatory host responses with yet poorly understood mechanistic basis. We previously reported that HCA2 in A431 epithelial cells transduced Gβγ-protein kinase C- and Gβγ-metalloproteinase/EGFR-dependent MAPK/ERK signaling cascades. Here, we investigated the role...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42279
更新日期：2017-02-10 00:00:00
abstract：:This study was to investigate effects of progesterone vaginal sustained-release gel and intramuscular injection of progesterone on frozen-thawed embryos transfer in 3013 patients receiving vaginal progesterone sustained-release gel and progesterone injection in artificial cycle for frozen-thawed embryo transfer. All p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51717-5
更新日期：2019-10-24 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-61010-5
更新日期：2020-03-03 00:00:00
abstract：:In this work, a broadband and broad-angle polarization-independent random coding metasurface structure is proposed for radar cross section (RCS) reduction. An efficient genetic algorithm is utilized to obtain the optimal layout of the unit cells of the metasurface to get a uniform backscattering under normal incidence...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40782
更新日期：2017-01-20 00:00:00
abstract：:We report on the intriguing evolution of the dynamical spin correlations of the frustrated spinel ZnMn2O4. Inelastic neutron scattering and magnetization studies reveal that the dynamical correlations at high temperatures are 1D. At lower temperature, these dynamical correlations become 2D. Surprisingly, the dynamical...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17771
更新日期：2015-12-08 00:00:00
abstract：:Inorganic semiconductors such as III-V materials are very important in our everyday life as they are used for manufacturing optoelectronic and microelectronic components with important applications span from energy harvesting to telecommunications. In some applications, these components are required to operate in hars...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21864-2
更新日期：2018-02-23 00:00:00
abstract：:The identification of the modes of action of bioactive compounds is a major challenge in chemical systems biology of diseases. Genome-wide expression profiling of transcriptional responses to compound treatment for human cell lines is a promising unbiased approach for the mode-of-action analysis. Here we developed a n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40164
更新日期：2017-01-10 00:00:00
abstract：:Sensorimotor transformation is indispensable to the accurate motion of the human body in daily life. For instance, when we grasp an object, the distance from our hands to an object needs to be calculated by integrating multisensory inputs, and our motor system needs to appropriately activate the arm and hand muscles t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09648
更新日期：2015-04-29 00:00:00
abstract：:Although copy number variation (CNV) and presence-absence variation (PAV) have been discovered in selected gene families in most crop species, the global prevalence of these polymorphisms in most complex genomes is still unclear and their influence on quantitatively inherited agronomic traits is still largely unknown....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61228-3
更新日期：2020-03-05 00:00:00
abstract：:The tick receptor for outer surface protein A (TROSPA) is the only identified factor involved in tick gut colonization by various Borrelia species. TROSPA is localized in the gut epithelium and can recognize and bind the outer surface bacterial protein OspA via an unknown mechanism. Based on earlier reports and our la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25205
更新日期：2016-04-26 00:00:00
abstract：:Andrographolide is a traditional herb medicine, widely used in Asia for conditions involving inflammation. The andrographlide-lipoic acid conjugate, AL-1, has been found being able to alleviate inflammation in our previous reports. Although the anti-inflammatory activity of AL-1 contributes to its cytoprotective effec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29716
更新日期：2016-07-20 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-63944-2
更新日期：2020-04-22 00:00:00
abstract：:Previous studies demonstrated distinct neural correlates underpinning impaired self-regulation (dysregulation) between individuals with autism spectrum disorder (ASD) and typically developing controls (TDC). However, the impacts of dysregulation on white matter (WM) microstructural property in ASD and TDC remain uncle...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70836-y
更新日期：2020-08-14 00:00:00
abstract：:Rapid histopathological evaluation of fresh, unfixed human tissue using optical sectioning microscopy would have applications to intraoperative surgical margin assessment. Microscopy with ultraviolet surface excitation (MUSE) is a low-cost optical sectioning technique using ultraviolet illumination which limits fluore...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22264-2
更新日期：2018-03-14 00:00:00
abstract：:We present and demonstrate an approach for femtosecond laser processing by using patterned vector optical fields (PVOFs) composed of multiple individual vector optical fields. The PVOFs can be flexibly engineered due to the diversity of individual vector optical fields in spatial arrangement and distribution of states...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02281
更新日期：2013-01-01 00:00:00
abstract：:In the present work, the mechanisms involved in the recently reported antiviral activity of zebrafish C-reactive protein-like protein (CRP1-7) against the spring viraemia of carp rhabdovirus (SVCV) in fish are explored. The results neither indicate blocking of the attachment or the binding step of the viral replicatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57501-0
更新日期：2020-01-17 00:00:00
