Abstract:
:Tripterygium wilfordii Hook. f. is the traditional medicinal plants in China. Triptolide, wilforgine, and wilforine are the bioactive compounds in T. wilfordii. In this study, the contents of three metabolites and transcription levels of 21 genes involved in three metabolites biosynthesis in T. wilfordii were examined using high-performance liquid chromatography and reverse transcription PCR after application of methyl jasmonate (MeJA) on hairy roots in time course experiment (3-24 h). The results indicated that application of MeJA inhibited triptolide accumulation and promoted wilforgine and wilforine metabolites biosynthesis. In hairy roots, wilforgine content reached 693.36 μg/g at 6 h after adding MeJA, which was 2.23-fold higher than control. The accumulation of triptolide and wilforine in hairy roots increased the maximum at 9 h, which was 1.3- and 1.6-folds more than the control. Most of the triptolide secretes into the medium, but wilforgine and wilforine cannot secrete into the medium. The expression levels of unigenes which involved terpenoid backbone biosynthesis exist the correlation with marker metabolites (triptolide, wilforgine and wilforine) after induction by MeJA, and can be then used to infer flux bottlenecks in T. wilfordii secondary metabolites accumulation. These results showed that these genes may have potential applications in the metabolic engineering of T. wilfordii metabolites production.
journal_name
J Asian Nat Prod Resjournal_title
Journal of Asian natural products researchauthors
Zhang J,Huo YB,Liu Y,Feng JT,Ma ZQ,Zhu CS,Zhang Xdoi
10.1080/10286020.2016.1232713subject
Has Abstractpub_date
2017-08-01 00:00:00pages
823-832issue
8eissn
1028-6020issn
1477-2213journal_volume
19pub_type
杂志文章abstract::Four new compounds, uvamalols A-C and uvarimacrophin A, have been isolated from the roots of Uvaria macrophylla. Their structures have been elucidated by spectroscopic methods. The relative configurations of uvamalols A-C have been established by NOE experiments, and the relative stereochemistry of uvarimacrophin A in...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020412331286524
更新日期:2005-10-01 00:00:00
abstract::Epoxyeicosatrienoic acids (EETs) and their regulating enzyme soluble epoxide hydrolase (sEH) have been associated with ischemic stroke. Salvianolic acid A (SAA) is proved to display potent cerebroprotection. However, little information is available about the link between them. This study aimed to investigate whether S...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2012.723200
更新日期:2012-01-01 00:00:00
abstract::A new bioactive flavone glycoside 1 [mp 252-254 degrees C, C28H32O16, [M]+ 624 (EIMS)] was isolated from the acetone soluble fraction of the defatted seeds of Cassia fistula (Linn.). It was characterized as a new bioactive flavone glycoside 5,3',4'-tri-hydroxy-6-methoxy-7-O-alpha-L-rhamnopyranosyl-(1 --> 2)-O-beta-D-g...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/1028602031000080478
更新日期:2003-03-01 00:00:00
abstract::Two new sesquiterpenoids, 4,5-epoxy-13-methoxy-1(10)-germacren-12,6-olide and 4,5-epoxy-13-acetoxy-1(10)-germacren-12,6-olide, were isolated from the leaves of Magnolia grandiflora, together with six known compounds, 2alpha-hydroxy-dihydroparthenolide, parthenolide, costunolide, syringaresinol, (+) medioresinol and 6,...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020108041376
更新日期:2001-01-01 00:00:00
abstract::A new oleanane type triterpene characterized as 3α, 24-diacetoxy-12-oleanene (1) together with known compounds 2-10, were isolated from Annona mucosa leaf extracts. Their structural elucidation was accomplished using physical and spectroscopic methods. The compounds showed weak to moderate insecticidal activities agai...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2020.1818726
更新日期:2020-09-15 00:00:00
abstract::Three new sesquiterpenoids (1-3), along with four known compounds (4-7), were isolated from cultures of the fungus Conocybe siliginea. The structures of new compounds were elucidated using spectroscopic methods. The known compounds were identified by comparing their spectroscopic data with those reported in the litera...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2014.939072
更新日期:2015-01-01 00:00:00
abstract::A new chiro-inositol ester, 4-hydroxyphenylacetyl-3-d-chiro-inositol ester (1), was isolated from the whole plants of Prenanthes macrophylla Franch., along with 10 known compounds, 4-hydroxyphenylacetic acid (2), trans-ethyl caffeate (3), cis-ethyl caffeate (4), protocatechualdehyde (5), luteolin (6), luteolin-7-O-β-d...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2011.635142
更新日期:2012-01-01 00:00:00
abstract::From the EtOH extract of the flowers of Camellia nitidissima Chi, a new acylated flavonoid glycoside, quercetin 7-O-(6"-O-E-caffeoyl)-β-D-glucopyranoside (1), has been isolated, together with three known flavonoids: quercetin (2), quercetin 3-O-β-D-glucopyranoside (3), and quercetin 7-O-β-D-glucopyranoside (4). Their ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2012.691475
更新日期:2012-01-01 00:00:00
abstract::3,7-Dimethyl-7-hydroxy-2-octen-1,6-olide and 3,7-dimethyl-2,6-octadien-1,6-olide, the natural bioactive compounds isolated from the fruit of Litsea cubeba and the liverwort Plagiochila rutilans, were totally synthesized using easily available cis-geraniol as raw material in short, convenient, and low-cost, five-step r...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2013.803474
更新日期:2013-01-01 00:00:00
abstract::A new furanocoumarin named lucidafuranocoumarin A (7) together with 13 known coumarins (1-6, 8-14) and four known alkaloids (15-18) was isolated from the roots of Feroniella lucida. Their structures were elucidated on the basis of spectroscopic analysis. Some of the isolates were evaluated for their biological activit...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2011.572878
更新日期:2011-06-01 00:00:00
abstract::By various chromatographic methods, one new phenylpropanoid, acanthopanic acid (1), and three known compounds, 1,2-O-dicaffeoylcyclopenta-3-ol (2), (4S)-α-terpineol 8-O-β-D-glucopyranoside (3), and rutin (4), were isolated from the methanol extract of the Acanthopanax koreanum leaves. Their structures were elucidated ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2010.525743
更新日期:2011-01-01 00:00:00
abstract::Phytochemical study on the leaves of Rhodomyrtus tomentosa resulted in the isolation of fourteen compounds including a new acylphloroglucinol, named tomentosone C (1), and a new flavonol glycoside, namely myricetin-3,7,3'-trimethyl ether-5'-O-β-glucopyranoside (2). Their structures were characterized by spectral data ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2015.1121997
更新日期:2016-06-01 00:00:00
abstract::A polysaccharide Astragalus polysaccharide (APS) was obtained from the boiling water extract of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao. The results of gas chromatography (GC) indicated that APS consisted of l-rhamnose (l-Rha), d-xylose (d-Xyl), d-glucose (d-Glc), and d-galactose (d-Gal) in...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2013.778832
更新日期:2013-01-01 00:00:00
abstract::Phytochemical analysis of the MeOH extract of the fruiting bodies of Stropharia aeruginosa resulted in the isolation of three lanostane triterpenoids, aeruginosols A, B and C. The structures of the new compounds were determined by spectroscopic analysis. The biological activities of aeruginosols A, B and C were examin...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020600882254
更新日期:2007-09-01 00:00:00
abstract::Vernonia travancorica Hook. f. is a taxon, endemic to the evergreen forests of Western Ghats (above 1000 m), India. From the aqueous EtOH extract of the air-dried corymbose inflorescence a novel secondary metabolite, 3-O-beta-[beta-(6'''-acetyl)-D-glucopyranosyl(1-->2)]-D-glucopyranosyl kaempferol (1), has been isolat...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/1028602031000147401
更新日期:2004-12-01 00:00:00
abstract::Dehydrozingerone analogs and related compounds were screened as potential antitumor promoters by using the in vitro short-term 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus early antigen activation assay. Among the 40 synthesized compounds, the prenylated analogs 16 and 34-36 showed the most si...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286021003591617
更新日期:2010-03-01 00:00:00
abstract::Camellia oleifera is expected to provide alternative aglycone to synthesize some saponins similar to that from Schima superba with inhibitory activity against Magnaporthe oryzae. Eight theasapogenol galactosides were synthesized via protection of adjacent hydroxyl groups by a benzylidene for regioselective glycosylati...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2017.1325877
更新日期:2018-02-01 00:00:00
abstract::Two new abietane diterpene glycosides, wilfordosides A (1) and B (2), were isolated from the roots of Tripterygium wilfordii. The structures of compounds 1 and 2 were established using spectroscopic methods including extensive 1D and 2D NMR analysis, in combination with chemical reactions. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2014.1001379
更新日期:2015-01-01 00:00:00
abstract::An ongoing phytochemical investigation of the rhizomes of Atractylodes lancea resulted in the isolation of four new C10-type polyacetylene glycosides (1-4). Their structures were elucidated on the basis of extensive spectroscopic data (UV, IR, 1D and 2D NMR, and HRESIMS). The absolute configurations of compounds 2-4 w...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2016.1247811
更新日期:2017-02-01 00:00:00
abstract::Anemarchalconyn (1) and anemarcoumarin A (2), the natural bioactive compounds isolated from the rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae), were first totally synthesized using easily available materials in short, convenient routes with overall yields of 32 and 48%. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2016.1275583
更新日期:2017-09-01 00:00:00
abstract::Two new lignans named neourticol A (1) and neourticol B (2), together with seven known compounds (3-9), were isolated from Urticae Fissae Herba, a folk medicine for rheumatism arthritis in China. The active evaluation results showed that 1 and 2 possessed the potent anti-complement and anti-inflammatory activities. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2018.1457652
更新日期:2019-06-01 00:00:00
abstract::Phytochemical investigation on the leaves of Rabdosia serra led to the isolation of two new abietane diterpenoids, raserranes A (1) and B (2), and four known phenylpropanoids (3-6). Their structures were determined by spectroscopic data. Compound 1 represented the rare examples of abietane diterpenoids featuring a 16-...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2018.1539081
更新日期:2020-01-01 00:00:00
abstract::Two new acylgluconic acids, trans-feruloylgluconic acid (1) and trans-caffeoylgluconic acid (2), together with three known compounds, myo-inositol (3), phthalic acid dibutyl ester (4), and wuzhuyuamide-I (5), were isolated from the water soluble part of the dried nearly ripe fruits of Evodia rutaecarpa (Juss.) Benth. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020802031080
更新日期:2008-07-01 00:00:00
abstract::Two new sesquiterpene derivatives, (+)-8β-hydroxy-longicamphenylone (1) and 11β-hydroxy-13-isopropyl-dihydrodehydrocostus lactone (2), were isolated from Chinese eaglewood. Their structures were elucidated on the basis of spectroscopic analyses including HR-ESI-MS, (1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HSQC, HMBC,...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2012.704910
更新日期:2012-01-01 00:00:00
abstract::A new coumestan named bavacoumestan D (1), together with five known compounds (2-6), was isolated from EtOAc-soluble extract of seeds of Psoralea corylifolia. Their structures were elucidated on the basis of spectroscopic and physico-chemical analyses. All compounds were evaluated for in vitro inhibitory activity agai...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2018.1563073
更新日期:2020-03-01 00:00:00
abstract::A novel tetraterpenoid named methyl tortuoate C (1) has been isolated from the soft coral Sarcophyton tortuosum Tix.-Dur. The structure of 1 was determined on the basis of spectroscopic methods. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020600604344
更新日期:2007-04-01 00:00:00
abstract::Three new alkylated benzoquinones, 2-hydroxy-5-ethoxy-3-nonyl-1,4-benzoquinone (1), 5-O-butyl-embelin (2), and 2,5-dihydroxy-6-methyl-3-pentadecyl-1,4-benzoquinone (3), together with seven known analogues (4-10), were isolated from the stems and twigs of mangrove plant, Aegiceras corniculatum. Their structural elucida...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2018.1540604
更新日期:2020-02-01 00:00:00
abstract::Four new lignan glycosides (1-4), named procumbenosides I, K, L, and M, together with cleistanthin B (5) reported for the first time in the genus Justicia, and 5 other known arylnaphthalene lignan glycosides (6-10) were isolated from the whole plant of Justicia procumbens. The structures of the new compounds were eluc...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2016.1241771
更新日期:2017-01-01 00:00:00
abstract::Paniculacin (1), a new coumarin derivative, has been isolated from the ethyl acetate soluble fraction of the ethanolic extract of Murraya paniculata along with umbelliferone, scopoletin, 4-hydroxybenzoic acid, trans-cinnamic acid, and β-sitosterol. Their structures were elucidated on the basis of spectral data. ...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2011.586343
更新日期:2011-08-01 00:00:00
abstract::A chemical investigation of the endemic relict shrub Tetraena mongolica led to the isolation of four new triterpenes: 11α,12α:13β,28-diepoxyoleanane-3β-yl trans-caffeate (1), 3β-hydroxy-11α,12α-epoxyoleanane-28-al (2), olean-11-en-28-al-3β-yl trans-caffeate (3), and 28-acetoxy-olean-12-en-3β-yl trans-caffeate (4). The...
journal_title:Journal of Asian natural products research
pub_type: 杂志文章
doi:10.1080/10286020.2012.699525
更新日期:2012-01-01 00:00:00