Abstract:
:Patent ductus arteriosus (PDA) can cause morbidity and mortality in neonates. Vascular remodeling, characterized by proliferation and migration of smooth muscle cells (SMCs), is an essential process for postnatal DA closure. Notch signaling is an important mediator of vascular remodelling but its role in DA is unkonwn. We investigated the effects and underlying mechanisms of γ-secretase inhibitor DAPT, a Notch signaling inhibitor on angiotensin II (Ang II)-induced proliferation and migration of DASMCs. Proliferation and migration of DASMCs cultured from neonatal Wistar rats were induced by Ang II, with or without DAPT pre-treatment. In addition, potential underlying mechanisms including cell cycle progression, Ca(2+) influx, reactive oxygen species (ROS) production, signal transduction of MAPK and Akt, and Notch receptor with its target gene pathway were examined. We found that DAPT inhibited Ang II-induced DASMCs proliferation and migration dose dependently. DAPT also arrested the cell cycle progression in the G0/G1-phase, and attenuated calcium overload and ROS production caused by Ang II. Moreover, DAPT inhibited nuclear translocation of Notch3 receptor intracellular domain, with decreased expression of its down-stream genes including HES1, HES2 and HES5. Finally, Ang II-activated ERK1/2, JNK and Akt were also counteracted by DAPT. In conclusion, DAPT inhibits Ang II-induced DASMCs proliferation and migration. These effects are potentially mediated by decreased calcium influx, reduced ROS production, and down-regulation of ERK1/2, JNK and Akt, through the Notch3-HES1/2/5 pathway. Therefore, Notch signaling has a role in DA remodeling and may provide a target pathway for therapeutic intervention of PDA.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Wu JR,Yeh JL,Liou SF,Dai ZK,Wu BN,Hsu JHdoi
10.7150/ijbs.16430subject
Has Abstractpub_date
2016-07-18 00:00:00pages
1063-73issue
9issn
1449-2288pii
ijbsv12p1063journal_volume
12pub_type
杂志文章abstract::Obesity and type 2 diabetes mellitus are complicated metabolic diseases that affect multiple organs and are characterized by hyperglycaemia. Currently, stable and effective treatments for obesity and type 2 diabetes mellitus are not available. Therefore, the mechanisms leading to obesity and diabetes and more effectiv...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.27173
更新日期:2018-08-06 00:00:00
abstract::Endoplasmic reticulum (ER) stress-induced neuronal apoptosis is a crucial pathological process of spinal cord injury (SCI). In our previous study, icariin (ICA) showed neuroprotective effects in SCI. However, the relationships between ER stress and ICA in SCI are unclear yet. Therefore, whether ICA could ameliorate SC...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.30348
更新日期:2019-01-01 00:00:00
abstract::Angiogenesis plays an important role in myocardial repair after myocardial infarction (MI). Cardiac micro-vascular endothelial cells (CMECs) are important participants in myocardial angiogenesis processes. Recent studies have revealed that Nuclear factor-erythroid 2-related factor 2 (Nrf2), a master transcription fact...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5887
更新日期:2013-07-07 00:00:00
abstract::Background: There is no information regarding transarterial chemoembolization (TACE) as a postoperative management after hepatic resection for patients with hepatitis B virus (HBV)-associated intrahepatic cholangiocarcinoma (ICC). Methods: Forty-two patients with pathological confirmation of HBV-associated ICC were en...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.21149
更新日期:2017-09-21 00:00:00
abstract::Tyrosylprotein sulfotransferase (TPST), responsible for the sulfation of a variety of secretory and membrane proteins, has been identified and characterized in submandibular salivary glands (William et al. Arch Biochem Biophys 1997; 338: 90-96). In the present study we demonstrate the sulfation of a salivary secretory...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3.237
更新日期:2007-03-02 00:00:00
abstract::Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has an urgent need for more effective treatment. Recently, chitinase 3 like 1 (Chi3L1) expression has been found in a variety of cancer cells. However it is not known whether Chi3L1 regulates the STAT3 pathway in OS cells. Here...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.37781
更新日期:2020-01-01 00:00:00
abstract::Upon salinity challenge, the Na(+)-K(+)-ATPase (NKA) of fish kidney plays a crucial role in maintaining ion and water balance. Moreover, the FXYD protein family was found to be a regulator of NKA. Our preliminary results revealed that fxyd12 was highly expressed in the kidneys of the two closely related euryhaline med...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.15066
更新日期:2016-04-28 00:00:00
abstract::Gene silencing techniques are widely used to control gene expression and have potential for RNAi-based therapeutics. In this report, transgenic mouse lines were created for conditional knockdown of Srsf3 (SRp20) expression in liver and mammary gland tissues by expressing Srsf3-specific shRNAs driven by a U6 promoter. ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13011
更新日期:2015-09-03 00:00:00
abstract::Although it has been widely applied in identification of genes responsible for biomedically, economically, or even evolutionarily important complex and quantitative traits, traditional candidate gene approach is largely limited by its reliance on the priori knowledge about the physiological, biochemical or functional ...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.3.420
更新日期:2007-10-25 00:00:00
abstract::Genetic variant T329S in flavin-containing monooxygenase 3 (FMO3) impairs trimethylamine (TMA) metabolism in birds. The TMA metabolism that under complex genetic and dietary regulation, closely linked to cardiovascular disease risk. We determined whether the genetic defects in TMA metabolism may change other metabolic...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.16472
更新日期:2016-10-25 00:00:00
abstract::The powerful pro-angiogenic capacity of human amnion-derived mesenchymal stem cells (hAMSCs) could be a valuable therapeutic angiogenesis strategy for bone regeneration. However, the molecular mechanisms underlying this process remain largely unknown. Herein, we report upregulated expression of circular RNA 100290 (ci...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.39895
更新日期:2020-05-18 00:00:00
abstract::Thymosin alpha 1 (Tα1) has immunomodulatory and anti-tumor effects in patients and has been commercialized in worldwide. An innovative technique is therefore impending to achieve high-yield expression and purification of Tα1 to meet the increasing requirements for clinical applications. Tα1 can enhance T cells, dendri...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.618
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Increasing evidences suggest that innate immunity is involved in cerebral ischemia-reperfusion (I/R) injury, but the liable innate immune receptors have not been completely elucidated. Here, we explored the role of the nucleotide-binding oligomerization domain (NOD)2, a member of the cytosolic NOD-like recep...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.10927
更新日期:2015-03-25 00:00:00
abstract::Statins, known as HMG-CoA reductase (HMGCR) inhibitors, have primarily been utilized for metabolic and angiographic medical applications because of their cholesterol-lowering effects. Similar to other drugs, statins may also induce a series of potential side effects. Statins inhibit the HMGCR (rate-limiting enzyme) ac...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.42965
更新日期:2020-08-13 00:00:00
abstract::Sirtuins are highly conserved NAD+-dependent protein deacetylases and/or ADP-ribosyltransferases that can extend the lifespan of several lower model organisms including yeast, worms and flies. The seven mammalian sirtuins, SIRT1 to SIRT7, have emerged as key metabolic sensors that directly link environmental signals t...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.7.575
更新日期:2011-01-01 00:00:00
abstract::The loss of wild type p53 tumor suppressive function and oncogenic gain-of-function of p53 mutants have been showing important implications in tumorigenesis. The p53(N236S) (p53(N239S) in human, p53S) mutation has been shown to lose wild type p53 function by yeast assay. However, its gain of function is still not clea...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4176
更新日期:2012-01-01 00:00:00
abstract::Our previous studies indicated that phosphoinositide specific phospholipase Cγ1 (PLCγ1) was involved in autophagy induction in colon and hepatic carcinoma cells. However, whether and how PLCγ1 regulation in human lung adenocarcinoma is linked to autophagy remains unclear. Here, we assessed the protein expression of PL...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.42962
更新日期:2020-02-21 00:00:00
abstract::MiR-216a-5p has opposite effects on tumorigenesis and progression in the context of different tumors, acting as either a tumor suppressor or an oncogene. However, the expression and function of miR-216a-5p in pancreatic cancer (PC) is not well characterized. In this study, we found miR-216a-5p was significantly downre...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.46822
更新日期:2020-07-19 00:00:00
abstract::Glucocorticoid medication is one of the most common causes of atraumatic osteonecrosis of the femoral head (ONFH), and vitamin K2 (VK2) has been shown to play an important and beneficial role in bone metabolism. In this study, we hypothesized that VK2 could decrease the incidence of glucocorticoid-induced ONFH in a ra...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13269
更新日期:2016-01-28 00:00:00
abstract::The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in multiple types of tum...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.25928
更新日期:2018-05-21 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC) occurs with the highest frequency in China, especially in the high-risk Northern Chinese. Recent studies have reported that SLC22A3 is significantly downregulated in non-tumor (NT) esophageal tissues from familial ESCC patients compared with those from sporadic ESCC. However, ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.28482
更新日期:2018-09-07 00:00:00
abstract::β2-Adrenoceptor (β2-AR) agonists promote muscle growth. The aim of this study was to elucidate some effects of the selective β2-adrenoceptor agonist clenbuterol (CLB) on myoblast proliferation. We found that CLB induces cell cycle arrest in C2C12 myoblasts. This effect is partly due to the enhanced stability of p27, r...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.17948
更新日期:2017-10-17 00:00:00
abstract::Melanoma is an aggressive form of skin cancer characterized by rapid invasion and metastasis. CD147 is known to be functioning in cell invasion. In this study, we showed that CD147 was translocated from the cell membrane to the mitochondria in advanced melanoma. Melanoma patients with CD147 localized to the mitochondr...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.52043
更新日期:2021-01-01 00:00:00
abstract::Carbon monoxide (CO) is a vasoactive molecule that is generated by vascular cells as a byproduct of heme catabolism and it plays an important physiological role in circulation system. In order to investigate whether exogenous CO can mediate the growth and proliferation of vascular cells, in this study, we used 250 par...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4664
更新日期:2012-01-01 00:00:00
abstract::Wee1 kinase regulates the G2/M cell cycle checkpoint by phosphorylating and inactivating the mitotic cyclin-dependent kinase 1 (Cdk1). Loss of Wee1 in many systems, including yeast and drosophila, leads to premature mitotic entry. However, the developmental role of Wee1 in mammals remains unclear. In this study, we es...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.2.161
更新日期:2006-01-01 00:00:00
abstract::Dihydropyrimidinase-related protein 4 (Dpysl4) is a known regulator of hippocampal neuron development. Here, we report that Dpysl4 is involved in growth regulation, polarization and differentiation of dental epithelial cells during tooth germ morphogenesis. A reduction in Dpysl4 gene expression in the tooth germ produ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5510
更新日期:2013-04-26 00:00:00
abstract::The terminal loop region of primary miRNA transcripts is an important determinant controlling miRNA function in human, animals and plants. However, the effects of polymorphisms in loop region of miRNA precusors on phenotypic variation have not been reported, especially on agronomic traits in rice. From rice japonica v...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.6357
更新日期:2013-06-16 00:00:00
abstract::Lung cancer is one of the most common and deadly malignancies worldwide, in spite of advances in targeted therapy in recent years. An effective strategy for lung cancer prevention remains a major problem. Advances in next-generation sequencing have helped in understanding the RNA and identifying novel circular RNAs (c...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.39212
更新日期:2020-01-16 00:00:00
abstract::Ovarian cancer is the fifth common cause of death in woman worldwide. The tripartite motif-containing (TRIM) proteins consist of more than 70 known protein members. Studies have showed that TRIM proteins are involved in cancer and play important roles in cancer cell proliferation, migration, adhesion and metastasis. R...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.28757
更新日期:2018-11-03 00:00:00
abstract::Cirrhosis is the final outcome of liver fibrosis. Kupffer cell-mediated hepatic inflammation is considered to aggravate liver injury and fibrosis. Alternatively-activated macrophages are able to control chronic inflammatory events and trigger wound healing processes. Nevertheless, the role of alternative Kupffer cell ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.1273
更新日期:2011-01-01 00:00:00