Abstract：
:Cell polarity arises through the spatial segregation of polarity regulators. PAR proteins are polarity regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how the spatially segregated PAR proteins interact to maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals that cortical PAR-2 diffuses only short distances, and, as a result, most PAR-2 molecules associate and dissociate from the cortex without crossing into the opposing domain. Our results show that cortical PAR-2 asymmetry is maintained by the local exchange reactions that occur at the cortical-cytoplasmic boundary. Additionally, we demonstrate that local exchange reactions are sufficient to maintain cortical asymmetry in a parameter-free mathematical model. These findings suggest that anterior and posterior PAR proteins primarily interact through the cytoplasmic pool and not via cortical diffusion.
journal_name
Cell Repjournal_title
Cell reportsauthors
Arata Y,Hiroshima M,Pack CG,Ramanujam R,Motegi F,Nakazato K,Shindo Y,Wiseman PW,Sawa H,Kobayashi TJ,Brandão HB,Shibata T,Sako Ydoi
10.1016/j.celrep.2016.07.047subject
Has Abstractpub_date
2016-08-23 00:00:00pages
2156-2168issue
8issn
2211-1247pii
S2211-1247(16)30978-0journal_volume
16pub_type
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