Abstract:
:Reprogramming of somatic cells has become a versatile tool for biomedical research and for regenerative medicine. In the current study, we show that manipulating alternative splicing (AS) is a highly potent strategy to produce cells for therapeutic applications. We demonstrate that silencing of hTAF4-TAFH activity of TAF4 converts human facial dermal fibroblasts to melanocyte-like (iMel) cells. iMel cells produce melanin and express microphthalmia-associated transcription factor (MITF) and its target genes at levels comparable to normal melanocytes. Reprogramming of melanoma cells by manipulation with hTAF4-TAFH activity upon TAFH RNAi enforces cell differentiation towards chondrogenic pathway, whereas ectoptic expression of TAF4 results in enhanced multipotency and neural crest-like features in melanoma cells. In both cell states, iMels and cancer cells, hTAF4-TAFH activity controls migration by supporting E- to N-cadherin switches. From our data, we conclude that targeted splicing of hTAF4-TAFH coordinates AS of other TFIID subunits, underscoring the role of TAF4 in synchronised changes of Pol II complex composition essential for efficient cellular reprogramming. Taken together, targeted AS of TAF4 provides a unique strategy for generation of iMels and recapitulating stages of melanoma progression.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kazantseva J,Sadam H,Neuman T,Palm Kdoi
10.1038/srep30852subject
Has Abstractpub_date
2016-08-08 00:00:00pages
30852issn
2045-2322pii
srep30852journal_volume
6pub_type
杂志文章abstract::The finite-difference time-domain (FDTD) method was employed to simulate the electric field distribution for noble metal (Au or Ag)/semiconductor (Ge or Si) substrates. The simulation showed that noble metal/Ge had stronger SERS enhancement than noble metal/Si, which was mainly attributed to the different dielectric c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04052
更新日期:2014-02-11 00:00:00
abstract::Previous work has shown that human vision performs spatial integration of luminance contrast energy, where signals are squared and summed (with internal noise) over area at detection threshold. We tested that model here in an experiment using arrays of micro-pattern textures that varied in overall stimulus area and sp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29764
更新日期:2016-07-27 00:00:00
abstract::The acronymously named "ESKAPE" pathogens represent a group of bacteria that continue to pose a serious threat to human health, not only due to their propensity for repeated emergence, but also due to their ability to "eskape" antibiotic treatment. The evolution of multi-drug resistance in these pathogens alone has gr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41999
更新日期:2017-02-06 00:00:00
abstract::Flow rates play an important role in microfluidic devices because they affect the transport of chemicals and determine where and when (bio)chemical reactions occur in these devices. Flow rates can conveniently be determined using external peripherals in active microfluidics. However, setting specific flow rates in pas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53885-w
更新日期:2019-11-21 00:00:00
abstract::It has been reported that in patients with operable stage I non-small cell lung cancer (NSCLC), overall survival (OS) is better in those who undergo hypofractionated stereotactic radiation therapy (HSRT) than in those who undergo surgery. However, the reason that HSRT has a better OS has not been fully explored. Here,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04978-x
更新日期:2017-07-07 00:00:00
abstract::Hydrogen sulfide is the third and most recently discovered gaseous signaling molecule following nitric oxide and carbon monoxide, playing important roles both in normal physiological conditions and disease progression. The trimethylsulfonium ion (TMS) can result from successive methylation reactions of hydrogen sulfid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27038
更新日期:2016-06-01 00:00:00
abstract::The exovesicles (EVs) are involved in pathologic host-parasite immune associations and have been recently used as biomarkers for diagnosis of infectious diseases. The release of EVs by Trypanosoma cruzi, the causative agent of Chagas disease, has recently been described, with different protein cargoes including the MA...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44451
更新日期:2017-03-15 00:00:00
abstract::Many host specific mutations have been detected in influenza A viruses (IAVs). However, their effects on hydrogen bond (H-bond) variations have rarely been investigated. In this study, 60 host specific sites were identified in the internal proteins of avian and human IAVs, 27 of which contained mutations with effects ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14533-3
更新日期:2017-10-30 00:00:00
abstract::Giant optical transmittance changes of over 300% in wide wavelength range from 500 nm to 2500 nm were observed in LaBaCo2O5.5+δ thin films annealed in air and ethanol ambient, respectively. The reduction process induces high density of ordered oxygen vacancies and the formation of LaBaCo2O5.5 (δ = 0) structure evidenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37496
更新日期:2016-11-23 00:00:00
abstract::Colon cancer stem cells have been attributed to poor prognosis, therapeutic resistance and aggressive nature of the malignancy. Recent reports associated CD44v6 expression with relapse, metastasis and reduced 5-year survival of colon cancer patients, thereby making it a potential therapeutic target. Thus, in this stud...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35069-0
更新日期:2018-11-19 00:00:00
abstract::Alterations in the gut microbiota play a crucial role in host physiology and metabolism; however, the molecular pathways underlying these changes in diet-induced obesity are unclear. Mechanistic target of rapamycin (mTOR) signaling pathway is associated with metabolic disorders such as obesity and type 2 diabetes (T2D...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30887
更新日期:2016-07-29 00:00:00
abstract::α-Dystroglycanopathy (α-DGP) is a group of muscular dystrophy characterized by abnormal glycosylation of α-dystroglycan (α-DG), including Fukuyama congenital muscular dystrophy (FCMD), muscle-eye-brain disease, Walker-Warburg syndrome, and congenital muscular dystrophy type 1D (MDC1D), etc. LARGE, the causative gene f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08316
更新日期:2015-02-09 00:00:00
abstract::It remains unclear how infantile febrile seizures (FS) enhance adult seizure susceptibility. Here we showed that the transient increase of interleukin-1β (IL-1β) after prolonged FS promoted adult seizure susceptibility, which was blocked by interleukin-1 receptor antagonist (IL-1Ra) within a critical time window. Post...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21931
更新日期:2016-02-23 00:00:00
abstract::Studies suggest that chromium deficiency is associated with elevated levels of fasting blood glucose, circulating insulin, cholesterol and triglycerides, and decreased proportion of lean body mass. However, data directly relating chromium levels to metabolic syndrome (MetS) risk are lacking. A total of 3,648 American ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15606
更新日期:2015-10-22 00:00:00
abstract::Automatically detecting and grading cancerous regions on radical prostatectomy (RP) sections facilitates graphical and quantitative pathology reporting, potentially benefitting post-surgery prognosis, recurrence prediction, and treatment planning after RP. Promising results for detecting and grading prostate cancer on...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66849-2
更新日期:2020-06-18 00:00:00
abstract::In this work we have studied the effects of pharmacological concentrations of melatonin (1 µM-1 mM) on pancreatic stellate cells (PSC). Cell viability was analyzed by AlamarBlue test. Production of reactive oxygen species (ROS) was monitored following CM-H2DCFDA and MitoSOX Red-derived fluorescence. Total protein carb...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63433-6
更新日期:2020-04-14 00:00:00
abstract::The wheat avenin-like proteins (ALP) are considered atypical gluten constituents and have shown positive effects on dough properties revealed using a transgenic approach. However, to date the genetic architecture of ALP genes is unclear, making it impossible to be utilized in wheat breeding. In the current study, thre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30692
更新日期:2016-08-09 00:00:00
abstract::The intercellular spreading of protein assemblies is a major factor in the progression of neurodegenerative disorders. The quantitative study and visualization of cell-to-cell propagation using tagged-proteins is challenging due to the steric effect of relatively large fluorescence tags and the risk of 'false positive...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00657-z
更新日期:2017-03-28 00:00:00
abstract::Sorafenib is a multikinase inhibitor used as a first-line treatment for advanced hepatocellular carcinoma (HCC), but it has shown modest to low response rates. The characteristic tumour hypoxia of advanced HCC maybe a major factor underlying hypoxia-mediated treatment failure. Thus, it is urgent to elucidate the mecha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30483
更新日期:2016-08-01 00:00:00
abstract::Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code. Small molecules have been shown to artificially induce epigenetic modifications in a sequence independent manner. Recently, we showed that specific DNA binding hairpin pyr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00544
更新日期:2012-01-01 00:00:00
abstract::Estimating the potential distribution of invasive species has been primarily achieved by employing species distribution models (SDM). Recently introduced joint species distribution models (JSDM) that include species interactions are expected to improve model output. Here we compare the predictive ability of SDM and JS...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52256-9
更新日期:2019-11-01 00:00:00
abstract::Intergroup competition has an important impact on the survival and fitness of individuals in group-living species. However, factors influencing the probability of winning an encounter are not fully understood. We studied the influence of numerical advantage and location of the encounter on the chances of winning in ei...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28457
更新日期:2016-06-22 00:00:00
abstract::High sensitivity magnetoelectric sensors with their electromechanical resonance frequencies < 200 kHz have been recently demonstrated using magnetostrictive/piezoelectric magnetoelectric heterostructures. In this work, we demonstrate a novel magnetoelectric nano-electromechanical systems (NEMS) resonator with an elect...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01985
更新日期:2013-01-01 00:00:00
abstract::The deregulation of autophagy is involved in liver regeneration. Here, we investigated the role of autophagy in the regulation of liver regeneration after partial hepatectomy (PHx) and the development of pharmacological interventions for improved liver regeneration after PHx. We show that autophagy was activated in th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15807
更新日期:2015-10-30 00:00:00
abstract::Fluid strategy is the key to the successful management of patients with sepsis. However, previous studies failed to consider individualized treatment strategy, and clinical trials typically included patients with sepsis as a homogeneous study population. We aimed to develop sequential decision rules for managing fluid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74906-z
更新日期:2020-10-21 00:00:00
abstract::In this report, we investigate the stabilization of saddle fixed points in coupled oscillators where individual oscillators exhibit the saddle fixed points. The coupled oscillators may have two structurally different types of suppressed states, namely amplitude death and oscillation death. The stabilization of saddle ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45909
更新日期:2017-04-05 00:00:00
abstract::Neuroblastoma is the most commonly diagnosed extracranial tumor in the first year of life. Approximately 9% of neuroblastoma patients present germline or somatic aberrations in the gene encoding for anaplastic lymphoma kinase (ALK). This increases in high-risk neuroblastomas, which have a 14% frequency of ALK aberrati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55060-7
更新日期:2019-12-18 00:00:00
abstract::Behavioral alterations emerging after central or peripheral vision loss suggest that cerebral reorganization occurs for both the afferented and deafferented early visual cortex (EVC). We explored the functional reorganization of the central and peripheral EVC following visual field defects specifically affecting centr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43223
更新日期:2017-02-24 00:00:00
abstract::Ncb2, the β subunit of NC2 complex, a heterodimeric regulator of transcription was earlier shown to be involved in the activated transcription of CDR1 gene in azole resistant isolate (AR) of Candida albicans. This study examines its genome-wide role by profiling Ncb2 occupancy between genetically matched pair of azole...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46084
更新日期:2017-04-06 00:00:00
abstract::Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77846-w
更新日期:2020-12-18 00:00:00