Abstract：
AIM:Local levels of angiotensin peptides depend on their rates of production and degradation, which induce proatherogenic or atheroprotective effects. Here, we reveal the kinetics of Angiotensin-I metabolism in paired early and advanced atherosclerotic lesions. METHODS:Lesions were spiked with labeled Ang-I* and supernatants withdrawn after 0, 10, 20, 40 and 80min. The concentration of produced Ang-II*, Ang-III*, Ang-IV* and Ang-(1-7)* peptides were measured using multiple reaction monitoring mass spectrometry coupled to ultra-performance liquid chromatography, normalized to tissue weight and initial [Ang-I*]. RESULTS:Ang-(1-7)* was the major angiotensin peptide produced, showing increased levels in both tissue types, with 2-3 fold lower levels in advanced compared to early lesions. In contrast, Ang-II* was 2-3 fold higher in advanced compared to early lesions, showing a decrease between 0 and 40min then an increase at 80min in both tissue types. The levels of Ang-IV were stable in both tissue types across all time points. Finally, Ang-III was non-detectable in both lesions across all time points. CONCLUSION:Our results suggest that progression of atherosclerosis depends on the increased levels of Ang-II along with the decreased levels of Ang-(1-7), which supports the use of Ang-(1-7) along with Angiotensin type-1 receptor (AT1R) blockers.
journal_name
Vascul Pharmacoljournal_title
Vascular pharmacologyauthors
Nehme A,Marcelo P,Nasser R,Kobeissy F,Bricca G,Zibara Kdoi
10.1016/j.vph.2016.08.001subject
Has Abstractpub_date
2016-10-01 00:00:00pages
50-56eissn
1537-1891issn
1879-3649pii
S1537-1891(16)30120-3journal_volume
85pub_type
杂志文章abstract：:We recently demonstrated that lipoic acid suppresses endotoxin-stimulated expression of inducible nitric oxide synthase and nitric oxide production in mouse macrophages. In this study, we tested whether lipoic acid suppresses these inflammatory mediators in the lungs of rats. Rats were assigned to receive either no sp...
journal_title：Vascular pharmacology
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doi：10.1016/s1537-1891(02)00307-5
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journal_title：Vascular pharmacology
pub_type： 杂志文章,评审
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abstract：:There is little information about the direct effect of caffeine in human blood vessels. The purpose of this study was to evaluate the direct vascular effect of caffeine on human internal mammary artery (IMA) and the involvement of potassium channels in this response. Segments of IMA were obtained from 29 patients who ...
journal_title：Vascular pharmacology
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pub_type： 杂志文章,评审
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journal_title：Vascular pharmacology
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journal_title：Vascular pharmacology
pub_type： 杂志文章,评审
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journal_title：Vascular pharmacology
pub_type： 杂志文章
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journal_title：Vascular pharmacology
pub_type： 杂志文章
doi：10.1016/j.vph.2004.11.003
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journal_title：Vascular pharmacology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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doi：10.1016/j.vph.2008.06.001
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pub_type： 杂志文章,评审
doi：10.1016/j.vph.2013.12.003
更新日期：2014-02-01 00:00:00
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journal_title：Vascular pharmacology
pub_type： 杂志文章
doi：10.1016/j.vph.2006.10.002
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journal_title：Vascular pharmacology
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journal_title：Vascular pharmacology
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journal_title：Vascular pharmacology
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doi：10.1016/j.vph.2015.03.006
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doi：10.1016/j.vph.2018.09.003
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