Abstract：
:During development, two cell types born from closely related progenitor pools often express identical transcriptional regulators despite their completely distinct characteristics. This phenomenon implies the need for a mechanism that operates to segregate the identities of the two cell types throughout differentiation after initial fate commitment. To understand this mechanism, we investigated the fate specification of spinal V2a interneurons, which share important developmental genes with motor neurons (MNs). We demonstrate that the paired homeodomain factor Chx10 functions as a critical determinant for V2a fate and is required to consolidate V2a identity in postmitotic neurons. Chx10 actively promotes V2a fate, downstream of the LIM-homeodomain factor Lhx3, while concomitantly suppressing the MN developmental program by preventing the MN-specific transcription complex from binding and activating MN genes. This dual activity enables Chx10 to effectively separate the V2a and MN pathways. Our study uncovers a widely applicable gene regulatory principle for segregating related cell fates.
journal_name
Cell Repjournal_title
Cell reportsauthors
Clovis YM,Seo SY,Kwon JS,Rhee JC,Yeo S,Lee JW,Lee S,Lee SKdoi
10.1016/j.celrep.2016.06.100subject
Has Abstractpub_date
2016-08-09 00:00:00pages
1642-1652issue
6issn
2211-1247pii
S2211-1247(16)30869-5journal_volume
16pub_type
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