Abstract:
:This study was conducted to characterize a novel Fusarium species that caused leaf and stem spot on Agapanthus praecox (Agapanthus, African lily) in northern Italy and leaf rot and spot on the same host in Melbourne, Australia. Formally described as Fusarium agapanthi, this pathogen was analyzed using phenotypic, phytopathogenic, secondary metabolite, molecular phylogenetic and genomic data. Five strains were characterized, including one isolated in 1999 from symptomatic A. praecox in Saluzzo, Italy, and four in 2010 from diseased leaf tissue from the same host exhibiting leaf rot and spot symptoms in the Melbourne Gardens, Royal Botanic Gardens Victoria, Australia. Maximum parsimony and maximum likelihood molecular phylogenetic analyses of portions of six individual genes and the combined dataset all strongly supported F. agapanthi either as the earliest diverging genealogically exclusive lineage in the American Clade of the F. fujikuroi species complex, or alternatively a novel monotypic lineage sister to the American Clade. Koch's postulates were completed on dwarf blue- and large white-flowering varieties of A. praecox, where two isolates of F. agapanthi produced slowly spreading necrotic lesions when inoculated onto leaves and flower stems. Fusarium agapanthi is distinguished from other fusaria by the production of densely branched aerial conidiophores with polyphialides throughout the aerial mycelium on synthetic nutrient-poor agar. BLASTn searches of the F. agapanthi NRRL 31653 and NRRL 54464 (= VPRI 41787) genome sequences were conducted to predict sexual reproductive mode and mycotoxin potential. Results indicated that they possessed MAT1-2 and MAT1-1 idiomorphs, respectively, indicating that this species might be heterothallic. Furthermore, based on the presence of homologs of the bikaverin and fusarubin biosynthetic gene clusters in the F. agapanthi genomes, liquid chromatography-mass spectrometry analysis was conducted and confirmed production of these secondary metabolites in rice and corn kernel cultures of the fungus.
journal_name
Mycologiajournal_title
Mycologiaauthors
Edwards J,Auer D,de Alwis SK,Summerell B,Aoki T,Proctor RH,Busman M,O'Donnell Kdoi
10.3852/15-333subject
Has Abstractpub_date
2016-09-01 00:00:00pages
981-992issue
5eissn
0027-5514issn
1557-2536pii
15-333journal_volume
108pub_type
杂志文章相关文献
MYCOLOGIA文献大全abstract::Symbiotic microorganisms, such as mycorrhizal fungi, are known to associate with most plants; however members of the Cruciferae are an exception. We investigated nutrient exchange between a dark septate endophytic fungus, Heteroconium chaetospira, and Chinese cabbage plants (Cruciferae) in vitro. Chinese cabbage could...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.99.2.175
更新日期:2007-03-01 00:00:00
abstract::The genus Haligena (Halosphaeriales, Ascomycota), with two accepted species, is encountered frequently in marine habitats, especially on wood in temperate regions. Phylogenetic analyses of Haligena elaterophora (type species) and H. salina were undertaken, with partial large subunit ribosomal DNA sequences, to determi...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.97.4.804
更新日期:2005-07-01 00:00:00
abstract::A pyrenomycete featuring uniperitheciate stromata embedded in a subiculum and asci with iodine-positive apical ascal rings that bear scolecosporous ascospores is described as new. The fungus, Ophiorosellinia costaricensis, is known only from the type location in Costa Rica. It has been cultured, but no anamorph was di...
journal_title:Mycologia
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Rock-inhabiting fungi (RIF) are adapted to thrive in oligotrophic environments and to survive under conditions of abiotic stress. Under these circumstances, they form biocoenoses with other tolerant organisms, such as lichens, or with less specific phototrophic consortia of aerial algae or cyanobacteria. RIF are phylo...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1543510
更新日期:2019-01-01 00:00:00
abstract::Xylaria hypoxylon, the type species of Xylaria (Xylariaceae, Sordariomycetes), was first described by Linnaeus as Clavaria hypoxylon from Sweden. Saccardo and other mycologists assumed a cosmopolitan distribution for this species. However, contradictory reports in the literature on its morphoanatomical characters and ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-108
更新日期:2009-03-01 00:00:00
abstract::Acremonium is one of the largest and most complex genera of hyphomycetes. Its taxonomy is not yet resolved and the phylogenetic value of the most distinctive morphological features is unknown. The species of this genus produce conidia in chains or form slimy heads or both. We have studied a set of clinical and environ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/106.2.328
更新日期:2014-03-01 00:00:00
abstract::Soil fungal communities are responsible for carbon and nitrogen (N) cycling. The high complexity of the soil fungal community and the high proportion of taxonomically unidentifiable sequences confound ecological interpretations in field studies because physiological information is lacking for many organisms known only...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/16-086
更新日期:2016-11-01 00:00:00
abstract::We present a taxonomic and phylogenetic study of Puccinia species (rust fungi) infecting tribe Lycieae (Solanaceae), with focus on the New World taxa. Phylogenetic analyses using nuclear (nuc) rDNA 5.8S-ITS2 (ITS2) and mitochondrial (mt) cytochrome oxidase subunit 3 (CO3) show that Puccinia species occurring on Lyciae...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1478538
更新日期:2018-07-01 00:00:00
abstract::Three species of Cercophora were found during a survey of the biodiversity of microfungi in northwest Argentina. Cercophora argentina possesses a unique combination of morphological characters and is described as a new species, while C. costaricensis and C. solaris are reported as new records for Argentina. Other spec...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-005
更新日期:2011-11-01 00:00:00
abstract::Trichoderma harzianum is known as a cosmopolitan, ubiquitous species associated with a wide variety of substrates. It is possibly the most commonly used name in agricultural applications involving Trichoderma, including biological control of plant diseases. While various studies have suggested that T. harzianum is a s...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/14-147
更新日期:2015-05-01 00:00:00
abstract::A new nivicolous species of Physarum was discovered during the study of myxomycetes in the Patagonian Andes of South America. It is described herein under the name Physarum andinum. The species is characterized by stalked sporophores or more rarely sessile sporocarps or short plasmodiocarps. The sporocarps are strikin...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-397
更新日期:2013-01-01 00:00:00
abstract::Fusarium zanthoxyli and F. continuum are sister taxa that are the etiological agents of canker disease of prickly ash (Zanthoxylum bungeanum) in northern China. These two pathogens, together with F. torreyae, the causal agent of canker disease of the critically endangered conifer Florida torreya (Torreya taxifolia) fr...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2018.1491766
更新日期:2018-07-01 00:00:00
abstract::Strains of Ceratobasidium bicorne (anamorph uninucleate Rhizoctonia), causing root dieback in nursery-grown conifer seedlings, were fruited in the laboratory and the pairing interactions among sibling, single-basidiospore progeny were investigated. No mating reactions were observed. Instead, a high frequency of somati...
journal_title:Mycologia
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::To aid the development of compatible biocontrol inocula, a prescreening method for the prediction of compatibility of fungal antagonists was developed. Compatibility between 18 Clonostachys isolates with known antagonistic capabilities against Phytophthora palmivora was tested using intra- or interisolate pairings (du...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-095
更新日期:2010-09-01 00:00:00
abstract::Among insect-fungus relationships, xylose-fermenting Scheffersomyces yeasts are well known for their potential in utilizing wood hemicelluloses and their association with various wood-feeding insects. However, their specificity to host insects or strain-level diversity within host species has not been clearly elucidat...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2017.1382648
更新日期:2017-07-01 00:00:00
abstract::Six Nowakowskiella species from Brazil were identified and purified on corn meal agar (CMA) plus glucose and Peptonized Milk-Tryptone-Glucose (PmTG) media and placed into a phylogenetic framework for the genus. New sequence data are presented for four species: N. elongata, N. multispora, and N. ramosa and the new spec...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.1080/00275514.2019.1588583
更新日期:2019-05-01 00:00:00
abstract::Sclerotinia sclerotiorum is a necrotrophic plant-pathogenic fungus that infects more than 400 species of plants. In this study the nascent polypeptide-associated complex α subunit gene of S. sclerotiorum (SsNACα; accession No. XP_001593856.1) was cloned and characterized. The relative transcript expression of SsNACα a...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/14-250
更新日期:2015-11-01 00:00:00
abstract::Fungal endophytes are ubiquitous in healthy root tissue, but little is known about their ecosystem functions, including their ability to utilize organic nutrient sources such as proteins. Root-associated fungi may secrete proteases to access the carbon and mineral nutrients within proteins in the soil or in the cells ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/14-106
更新日期:2015-01-01 00:00:00
abstract::We found high diversity of vegetative compatibility (vc) types in native populations of the chestnut blight fungus, Cryphonectria parasitica, in Japan and China; almost every isolate was in a unique vc type. In Japan we found 71 vc types in a sample of 79 isolates pooled from six populations. Within two populations in...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.99.2.279
更新日期:2007-03-01 00:00:00
abstract::The Pleosporaceae is an important loculoascomycete family. There has been disagreement, however, regarding the taxonomic placement of many genera within this family. This study investigates phylogenetic relationships among the genera Cochliobolus, Kirschsteiniothelia, Leptosphaerulina, Macroventuria, Pleospora, Pyreno...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.98.4.571
更新日期:2006-07-01 00:00:00
abstract::Botr yosphaeria stevensii frequently has been associated with dieback and canker diseases of oak, mainly in the western Mediterranean area but more rarely in other regions. The species concept of B. stevensii has been unclear, and it is possible that some collections were identified incorrectly. A collection of fungal...
journal_title:Mycologia
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::We present a strategy to recover high molecular weight genomic DNA from large numbers of isolates of Phytophthora. Included are steps for generating mycelial mass in 24-well reuseable deep well plates, efficient lyophilization and disruption of the mycelium and genomic DNA extraction with 96-well glass fiber filter pl...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.98.3.514
更新日期:2006-05-01 00:00:00
abstract::The new species Myriophacidium ramosum and Neococcomyces erumpens are described from Rhododendron twigs in the eastern Himalayan Mountains in China. Both are members of the Rhytismatales. ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-092
更新日期:2009-05-01 00:00:00
abstract::Russian knapweed (Rhaponticum repens) is a rangeland weed pest in the western United States. One candidate fungus for biological control of R. repens is Puccinia acroptili, which causes a rust disease. Understanding the life cycle of candidate rust fungi for weed biological control is an essential component in risk as...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/08-215
更新日期:2010-01-01 00:00:00
abstract::The mantle of dead organic material ("canopy soil") associated with the mats of vascular and nonvascular epiphytes found on the branches of trees in the temperate rainforests along the southwestern coast of the South Island of New Zealand were examined for evidence of ectomycorrhizal fungi. DNA sequencing and cluster ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-307
更新日期:2013-01-01 00:00:00
abstract::A species of Labyrinthula that causes 'rapid blight' and death of turfgrass has been isolated and studied. We name this new species Labyrinthula terrestris and briefly summarize morphological characteristics and growth patterns of this pathogen of turfgrass. ...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.97.1.185
更新日期:2005-01-01 00:00:00
abstract::Gloeodontia xerophila sp. nov. is described and illustrated from material collected on dead xerophyte debris in the Canary Islands. This species is characterized by the odontioid hymenophore with short aculei, up to 2 mm long, dimitic hyphal system and amyloid, subglobose and verrucose spores, 4-5 x 3-4 microm. The ne...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/07-200r1
更新日期:2008-07-01 00:00:00
abstract::The inoculum of ectomycorrhizal (EM) fungi was examined in a 16 y long nitrogen fertilization experiment maintained in a temperate oak savanna. To measure EM fungal inoculum, bur oak seedlings were grown in three types of bioassays: (i) intact soil cores that measure inoculum such as spores, mycelia and mycorrhizal ro...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/mycologia.97.2.329
更新日期:2005-03-01 00:00:00
abstract::Gondwanamyces and its Custingophora anamorphs were first described from Protea infructescences in South Africa. Subsequently these unusual fungi were also found on Cecropia in Central America. During an investigation into the decline and death of native Euphorbia trees in South Africa, several fungal isolates resembli...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/11-166
更新日期:2012-03-01 00:00:00
abstract::The genus Agaricus is known for its medicinal and edible species but also includes toxic species that belong to section Xanthodermatei. Previous phylogenetic reconstruction for temperate species, based on sequence data of nuc rRNA gene (rDNA) internal transcribed spacers (ITS), has revealed two major groups in this se...
journal_title:Mycologia
pub_type: 杂志文章
doi:10.3852/14-076
更新日期:2014-11-01 00:00:00