Abstract:
INTRODUCTION:Infection accounts for over 40% of preterm premature rupture of the fetal membranes (PPROM), a major cause of preterm birth. Toll-like receptors (TLR) play key roles in pathogen surveillance but their expression and function in amnion mesenchymal cells (AMC) is unclear. The aims of this study were to determine the expression of all TLR isoforms and the effect of macrophage-activating lipoprotein-2 (MALP-2), derived from a common pathogen involved in PPROM, on human AMC. METHODS:AMC were isolated from normal, term amnion from repeat cesarean section. Semi-quantitative RT-PCR, immunocytochemistry, immunohistochemistry and western blotting were used to detect TLR isoform expression. Immunocytochemistry of NF-κB p65, pro-inflammatory cytokine secretion (ELISA), MTT assay, LDH assay, immunoblotting of cytosolic cytochrome c and cleaved caspase-3, and expression of 84 microRNAs by Qiagen miRNA PCR array were used to determine the functional effect of MALP-2 on AMC. RESULTS:TLR1-10 was detected in AMC, and protein expression of TLR2, 4, and 6 were confirmed. MALP-2 induced nuclear translocation of p65, reaching significance after 45 min (ANOVA, P < 0.05). MALP-2 did not cause apoptosis but did lead to significant secretion of IL-4, IL-6, and IL-8 (P < 0.05, 0.01, 0.001, respectively) and significant changes in miRNA-320a and miRNA-18a (P < 0.05). DISCUSSION:These results suggest that AMC elicit a pro-inflammatory response following stimulation with the known TLR2/6 ligand MALP-2. This data supports the idea that AMC express the innate immune system receptors that could help with immune surveillance during infection and contribute to inflammatory responses that lead to PPROM.
journal_name
Placentajournal_title
Placentaauthors
Sato BL,Collier ES,Vermudez SA,Junker AD,Kendal-Wright CEdoi
10.1016/j.placenta.2016.06.005subject
Has Abstractpub_date
2016-08-01 00:00:00pages
69-79eissn
0143-4004issn
1532-3102pii
S0143-4004(16)30124-2journal_volume
44pub_type
杂志文章相关文献
PLACENTA文献大全abstract:OBJECTIVES:To compare the distribution of placental protein 13 (PP13) in fetal and maternal blood and amnionic fluid and to correlate it with PP13 protein and mRNA in the placenta. METHODS:Umbilical arterial serum, amnionic fluid, maternal venous serum and placental tissues were collected from normal outcome pregnanci...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.09.006
更新日期:2011-02-01 00:00:00
abstract::Placental ultrasonographic, bio- and histochemical studies were performed on four unrelated fetuses affected with Niemann-Pick disease Type A, following prostaglandin-induced abortion at about the 19th week of gestation. An accumulation of sphingomyelin in the placentae of affected fetuses indicates the essential role...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(85)80030-8
更新日期:1985-01-01 00:00:00
abstract::We have previously shown that thioredoxin and thioredoxin reductase were immunohistochemically localized in cytotrophoblasts, decidua and stromal cells in the stem villi of human placenta and that the addition of exogenous thioredoxin and thioredoxin reductase to mitochondrial fractions from human placenta displayed a...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.1999.0415
更新日期:1999-09-01 00:00:00
abstract::Intrauterine inflammation contributes to neonatal infection-related morbidity. A new histological framework of placental inflammation has recently been proposed; however, the association between this method and clinical findings has not been defined. To assess the clinical relevance of this system, we studied placenta...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.10.021
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:Decidual leukocytes are critical to the development of the fetomaternal interface, regulating tolerance to the semi-allogeneic fetus and vascular transformation of the uterine spiral arteries. Despite the continuation of these processes beyond the first trimester of pregnancy, the second trimester has larg...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.09.018
更新日期:2014-12-01 00:00:00
abstract::Preeclampsia is a leading cause of maternal and fetal morbidity and mortality in high and low-income countries. The aetiology of preeclampsia is multifactorial and remains obscure. Some evidences suggest that altered placental fatty acid oxidation might play a role in the pathogenesis of preeclampsia. To reveal if pla...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.11.027
更新日期:2012-02-01 00:00:00
abstract:INTRODUCTION:Knockout mouse model has shown a relationship between Slit2/Robo1 signalling and altered fertility. Altered expression by endometrial epithelium and trophoblast and is associated with the pathogenesis of pregnancy complications but few studies have investigated the expression of decidual Slit2 in miscarria...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2020.10.008
更新日期:2020-10-09 00:00:00
abstract::Maturation of the fetal hypothalamic-pituitary-axis may be an important factor in the stimulation of the onset of parturition. Recent studies have found that corticotrophin-releasing hormone (CRH), the hypothalamic component of this axis is also present in the placenta and the fetal membranes. We review the evidence d...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/0143-4004(91)90015-8
更新日期:1991-03-01 00:00:00
abstract::Glycogen storage disease type IV (GSD IV) is a rare autosomal recessive disorder caused by glycogen branching enzyme (GBE) deficiency and resulting in the storage of abnormal glycogen (polyglucosan). Prenatal diagnosis is based on biochemical assay of GBE activity or on mutation analysis, but polyglucosan can also be ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2008.01.005
更新日期:2008-04-01 00:00:00
abstract::In the present study the possible role of decidual cells in the pregnancy-associated increased sensitivity of glomerular ecto-ADP-ase to endotoxin was investigated. Early (day 5) pregnant (E-Pr; n = 10), pseudopregnant (E-PSP; n = 10), (day 5), pseudopregnant rats with a decidualized uterus (E-DEC; n - 10), as well as...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(96)80013-0
更新日期:1996-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression at the post-transcriptional level. While mostly intracellular, a portion of cellular miRNAs is released to the circulation and their level in the plasma is altered in certain pathological conditions such as cancer, and also during pregnancy. We...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.07.001
更新日期:2010-09-01 00:00:00
abstract::Stereological methods were used to test whether or not altitudinal, ethnic and sex differences in birthweight in Bolivia are associated with differences in placental morphology. Estimates of the volumes, surface areas, lengths and diameters of villi and of fetal capillaries were obtained by analysing paraffin sections...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(87)90077-4
更新日期:1987-09-01 00:00:00
abstract:INTRODUCTION:Visualization of the retroplacental clear space (RPCS) may provide critical insight into the development of abnormally invasive placenta (AIP). In this pre-clinical study, we characterized the appearance of the RPCS on magnetic resonance imaging (MRI) during the second half of gestation using a liposomal g...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.01.017
更新日期:2019-02-01 00:00:00
abstract::Sections of human term placentae delivered at low and high altitude to indigenous and non-indigenous populations in Bolivia were analysed by stereological methods. Estimates were obtained of the arithmetic and harmonic mean thicknesses of villous membrane and of trophoblast. These values were then used to derive indic...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(88)90067-7
更新日期:1988-01-01 00:00:00
abstract::The fusion of villous cytotrophoblast into the placental syncytium is the quintessential process in maintenance of a healthy pregnancy. Efficient fusion requires a phosphatidylserine (PS)-rich cytotrophoblast surface, expression of fusion proteins, cessation of cell cycling, and rearrangement of cytoskeleton to accomm...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2009.12.014
更新日期:2010-02-01 00:00:00
abstract:INTRODUCTION:The interaction between trophoblast cells and maternal uterine endothelium is important for placental vascular modelling. Nitric oxide (NO) is a potent vasorelaxant that regulates systemic blood pressure and is reduced in preeclampsia. NO may affect placental cell interaction. OBJECTIVES:This study was to...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.03.009
更新日期:2014-06-01 00:00:00
abstract::Although secretory phospholipase A2 (PLA2) isozymes have been identified in human gestational tissues, their role in homeostasis and pathophysiology during pregnancy has yet to be clearly established. The aims of this brief commentary are: (1) to review recent data concerning the expression of secretory PLA2 isozymes ...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/s0143-4004(98)90093-5
更新日期:1998-01-01 00:00:00
abstract::Human placental fragments concentrate 86Rb 10--20-fold during a two-hour incubation period. Inhibition of ouabain is dose-dependent, reaching 90 + per cent at a concentration of 5 x 10(-5) M. The clearance index of 86Rb across the perfused human placenta is 0.34 +/- 0.08, comparing to previously reported indices for N...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(83)80038-1
更新日期:1983-10-01 00:00:00
abstract::The aim of this study was to investigate localization and content of activin beta A-subunit and activin receptors in gestational tissues throughout pregnancy and in association with term labour. Placenta and fetal membranes were collected from women undergoing first and second trimester terminations and from women bef...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2002.0787
更新日期:2002-04-01 00:00:00
abstract::Early placental development is characterised by rapid cell differentiation and migration, matrix remodelling and angiogenesis. The enzyme NAD(P)H oxidase is a major source of superoxide anions implicated in signalling pathways regulating these processes in other systems. It is also thought to be involved in oxygen sen...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2005.02.013
更新日期:2006-02-01 00:00:00
abstract:INTRODUCTION:Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at t...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2018.02.003
更新日期:2018-04-01 00:00:00
abstract::Placental stem cells are of growing interest for a variety of clinical applications due to their multipotency and ready availability from otherwise frequently discarded biomaterial. Stem cells derived from the placenta have been investigated in a number of disease processes, including wound healing, ischemic heart dis...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2017.05.010
更新日期:2017-11-01 00:00:00
abstract::Paracellular pathways in the haemotrichorial placenta of the rat were studied by electron microscopy using lanthanum hydroxide as an electron dense marker. Near term placentae were dually perfused in situ, adding lanthanum to the fetal perfusate. In some placentae outflow pressure on the fetal side was elevated (betwe...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2000.0558
更新日期:2000-09-01 00:00:00
abstract::Chorioallantoic placentae were obtained from a reproductively synchronized wild population of greater mustache bats for ultrastructural and immunocytochemical examination. The single discoidal placenta was always located in a lateral to mesometrial position on the right side of the uterus, which in the non-pregnant st...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(98)90079-0
更新日期:1998-07-01 00:00:00
abstract:OBJECTIVE:To evaluate umbilical cord abnormalities predisposing to mechanical cord compression and determine their relationship to adverse clinical outcomes and stasis-associated histologic changes in the placenta. METHODS:Placental slides of 224 singleton pregnancies with gross cord abnormality (true knots, long cord...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.09.005
更新日期:2009-12-01 00:00:00
abstract::The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80451-5
更新日期:1994-02-01 00:00:00
abstract:INTRODUCTION:A major issue in the transcriptomic study of spontaneous preterm birth (sPTB) in humans is the inability to collect healthy control tissue at the same gestational age (GA) to compare with pathologic preterm tissue. Thus, gene expression differences identified after the standard comparison of sPTB and term ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2016.03.006
更新日期:2016-05-01 00:00:00
abstract::In view of the pathological placental features of pre-eclampsia and intrauterine growth restriction (IUGR), and the angiogenic effects of vascular endothelial growth factor (VEGF), the aim of this study was to determine the expression of VEGF in placentae from normal pregnancies and to compare the results with placent...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(97)80061-6
更新日期:1997-05-01 00:00:00
abstract::Human placental tissues have been shown to contain gonadotrophin-releasing hormone-(GnRH)-like activity. Thus, the effect of a potent GnRH antagonist (N-Ac-Pro1,D-p-Cl-Phe2,D-Nal(2)3,6-GnRH, obtained from Syntex Laboratories) on placental hormonal release was studied. Explant cultures of placentae of 6 to 15 weeks' ge...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(87)90035-x
更新日期:1987-01-01 00:00:00
abstract:INTRODUCTION:Placental blood flow is closely associated with fetal growth and wellbeing. Recent studies suggest that there are differences in blood flow between male and female fetuses. We hypothesized that sexual dimorphism exists in fetal and placental blood flow at 22-24 weeks of gestation. METHODS:This was a prosp...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2016.11.005
更新日期:2017-01-01 00:00:00