Abstract：
:This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture. Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively. Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues. Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Jamshidian H,Shojaosadati SA,Vilaplana F,Mousavi SM,Soudi MRdoi
10.1016/j.ijbiomac.2016.07.059subject
Has Abstractpub_date
2016-11-01 00:00:00pages
484-493eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30905-9journal_volume
92pub_type
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journal_title：International journal of biological macromolecules
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更新日期：2018-12-01 00:00:00
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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abstract：:Naturally produced polyhydroxyalkanoates (PHAs) biopolymers have limited medical applications due to their brittle and hydrophobic nature. In this study poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymer was produced using engineered Escherichia coli YJ101, and further functionalized with ascorbic ac...
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2019-05-15 00:00:00
abstract：:Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at -20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtaine...
journal_title：International journal of biological macromolecules
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更新日期：2020-08-01 00:00:00
abstract：:The ever-increasing applications of enzymes are limited by the relatively poor performance in harsh processing conditions. As a result, there are constant innovations in immobilization protocols for improving biocatalyst activity and stability. Bacterial spores are cheap to generate and highly resistant to environment...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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abstract：:There are different clotting factors present in blood, carries the clotting cascade and excessive bleeding may cause a deficiency in the clotting Diagnosis of this deficiency in clotting drastically reduces the potential fatality. For enabling a sensor to detect the clotting factors, suitable probes such as antibody a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2018.05.084
更新日期：2018-09-01 00:00:00
