Improving efficacy of cancer immunotherapy by genetic modification of natural killer cells.

abstract：BACKGROUND AIMS:The microenvironment of the chronically injured spinal cord does not allow for axonal regeneration due to glial scarring. To ameliorate this, several therapeutic strategies have been used. We investigated whether combined transplantation of chondroitinase ABC (chABC) and mesenchymal stromal cells (MSCs)...

journal_title：Cytotherapy

authors： Lee SH,Kim Y,Rhew D,Kim A,Jo KR,Yoon Y,Choi KU,Jung T,Kim WH,Kweon OK

更新日期：2016-10-28 00:00:00

abstract：BACKGROUND AIMS:Cytokine-induced killer (CIK) cells have shown cytolytic activity against several tumor cells in vitro and in animal tumor models. Furthermore, CIK cells activated by dendritic cell (DC) stimulation show increased anti-tumor activity. This study aimed to evaluate the clinical efficacy of DC-activated CI...

journal_title：Cytotherapy

authors： Li H,Wang C,Yu J,Cao S,Wei F,Zhang W,Han Y,Ren XB

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in di...

journal_title：Cytotherapy

authors： Lazzarotto-Silva C,Binato R,Rocher BD,Costa JA,Pizzatti L,Bouzas LF,Abdelhay E

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Dendritic cells (DCs) are the most efficient antigen-presenting cells and act at the center of the immune system owing to their ability to control both immune tolerance and immunity. In cancer immunotherapy, DCs play a key role in the regulation of the immune response against tumors and can be generated ex v...

journal_title：Cytotherapy

authors： Bulgarelli J,Fiammenghi L,Cassan S,Granato AM,Petrini M,Pancisi E,Soldati V,De Rosa F,Ridolfi L,Riccobon A,Guidoboni M

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

journal_title：Cytotherapy

authors： Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. M...

journal_title：Cytotherapy

authors： Zhong RK,Loken M,Lane TA,Ball ED

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of ...

journal_title：Cytotherapy

authors： Harting M,Jimenez F,Pati S,Baumgartner J,Cox C Jr

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled pers...

journal_title：Cytotherapy

authors： Zhu F,Shah N,Xu H,Schneider D,Orentas R,Dropulic B,Hari P,Keever-Taylor CA

更新日期：2018-03-01 00:00:00

abstract：BACKGROUND AIMS:Stem cell therapy for liver diseases has recently emerged as a promising alternative to liver transplantation. Eligible cells should have an appropriate immunophenotype. The aim of the present study was to define the immunological profile of two human liver-derived mesenchymal stromal cell populations, ...

journal_title：Cytotherapy

authors： Raicevic G,Najar M,Najimi M,El Taghdouini A,van Grunsven LA,Sokal E,Toungouz M

更新日期：2015-02-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

journal_title：Cytotherapy

authors： Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process o...

journal_title：Cytotherapy

authors： Serikov VB,Popov BV,Kropotov AV,Tomilin NV

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been studied intensively in regenerative medicine. However, their therapeutic potential against tumor formation and cancer metastasis is still unclear. The effects of transplantation of MSCs in early-stage of carcinogenesis, should be evaluated. METHODS:MSC isolated...

journal_title：Cytotherapy

authors： Sun B,Roh KH,Park JR,Lee SR,Park SB,Jung JW,Kang SK,Lee YS,Kang KS

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Stem cells derived from human adipose tissue (ASC) have the capacity for renewal, are easily obtained and have plasticity properties that allow them to differentiate into several cell types, including osteoblast cells. With the aim of understanding the issue of the osteogenic process and finding reliabl...

journal_title：Cytotherapy

authors： Giusta MS,Andrade H,Santos AV,Castanheira P,Lamana L,Pimenta AM,Goes AM

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:This study evaluated the feasibility, safety and immunological effects of the intravenous administration of mesenchymal stromal cells (MSCs) from a related donor in patients with refractory aplastic anemia (AA). METHODS:A mean of 6 × 10(5)/kg (range, 5.0-7.1 × 10(5)) MSCs were injected intravenously to...

journal_title：Cytotherapy

authors： Xiao Y,Jiang ZJ,Pang Y,Li L,Gao Y,Xiao HW,Li YH,Zhang H,Liu Q

更新日期：2013-07-01 00:00:00

abstract：:Infusion of viral-specific T cells (VSTs) is an effective treatment for viral infection after stem cell transplant. Current manufacturing approaches are rapid, but growth conditions can still be further improved. To optimize VST cell products, the authors designed a high-throughput flow cytometry-based assay using 40 ...

journal_title：Cytotherapy

authors： Lazarski CA,Datar AA,Reynolds EK,Keller MD,Bollard CM,Hanley PJ

更新日期：2021-01-01 00:00:00

abstract：BACKGROUND:[corrected] Recent findings in cord blood (CB) research indicate the potential clinical usefulness of IL-2-activated CB in eradication of minimal malignant residual disease after hematopoietic stem cell transplantation. This feasible approach to immunotherapy merits further pre-clinical investigations using ...

journal_title：Cytotherapy

authors： Derzic S,Slone V,Sender L

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...

journal_title：Cytotherapy

authors： Štach M,Ptáčková P,Mucha M,Musil J,Klener P,Otáhal P

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND AIMS:The aim was to evaluate cartilage regeneration in animal models involving induced knee joint damage. Through cell-mediated gene therapy methods, a cell mixture comprising a 3:1 ratio of genetically unmodified human chondrocytes and transforming growth factor beta-1 (TGF-beta1)-secreting human chondrocyt...

journal_title：Cytotherapy

authors： Noh MJ,Copeland RO,Yi Y,Choi KB,Meschter C,Hwang S,Lim CL,Yip V,Hyun JP,Lee HY,Lee KH

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND:Intracoronary application of BM-derived cells for the treatment of acute myocardial infarction (AMI) is currently being studied intensively. Simultaneously, strict legal requirements surround the production of cells for clinical studies. Thus good manufacturing practice (GMP)-compliant collection and prepara...

journal_title：Cytotherapy

authors： Griesel C,Heuft HG,Herrmann D,Franke A,Ladas D,Stiehler N,Stucki A,Braun M,Wollert KC,Meyer GP,Drexler H,Hertenstein B,Ganser A,Rüdinger W,Arseniev L

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...

journal_title：Cytotherapy

authors： Kumar G,Hara H,Long C,Shaikh H,Ayares D,Cooper DK,Ezzelarab M

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND:Traditionally, following high-dose therapy (HDT), unmanipulated autologous PBPC are infused. Alternatively, purified CD34+ cells can now be obtained by immunomagnetic separation using the CliniMACS device. Limited data currently exist examining hemopoietic recovery with such cells. METHODS:Ten patients with...

journal_title：Cytotherapy

authors： Prince HM,Wall D,Rischin D,Toner GC,Seymour JF,Blakey D,Haylock D,Simmons P,Wolf M,Januszewicz EH,Westerman D,Richardson G,Scarlett J,Briggs P

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:Hematopoietic progenitor cells (HPC) circulate at high levels at birth and disappear rapidly afterwards, but the underlying mechanism it is not known. The aim of this study was to assess circulating HPC in cord blood at different gestational ages and shortly after birth and concomitantly study the biologic m...

journal_title：Cytotherapy

authors： Gonzalez S,Amat L,Azqueta C,Madrigal JA,Laïlla JM,Garcia J,Querol S

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:To assess the impacts of stem cell therapy on clinical outcomes in patients with non-ischemic cardiomyopathy (NICM). The effect of stem cell therapy on prognosis is unclear and controversial. METHODS:The authors performed a systematic review and meta-analysis of the effects of autologous stem cell tran...

journal_title：Cytotherapy

authors： Xia L,Zeng L,Pan J,Ding Y

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND:Contaminating breast cancer cells in leukapheresis harvested for reinfusion to rebuild hemopoiesis after high-dose therapy have been described by several investigators. Methods for tumor cell detection are conventional immunocytochemistry, culture techniques and reverse transcriptase PCR. The percentage of t...

journal_title：Cytotherapy

authors： Krüger W,Tögel F,Rössing S,Kröger N,Zander AR

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:In vitro primary cultures of microvascular endothelial cells from endocrine pancreas are difficult to obtain, but can be a very helpful tool for studies of islet biology, transplantation and regenerative medicine. METHODS:We applied a protocol recently described for the isolation and culture of brain microv...

journal_title：Cytotherapy

authors： Sordi V,Ferri A,Ceserani V,Ciusani E,Dugnani E,Pellegrini S,Nano R,Pecciarini L,Pessina A,Pascucci L,Piemonti L,Alessandri G

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND AIMS:T cells can be redirected to reject cancer by retroviral transduction with a chimeric antigen receptor (CAR) or by administration of a bispecific T cell engager (BiTE). We demonstrate that transfection of T cells with messenger (m) RNA coding for CAR is an alternative strategy. METHODS:We describe the ...

journal_title：Cytotherapy

authors： Almåsbak H,Rian E,Hoel HJ,Pulè M,Wälchli S,Kvalheim G,Gaudernack G,Rasmussen AM

更新日期：2011-05-01 00:00:00

abstract：BACKGROUND AIMS:Shipment of therapeutic somatic cells between a current good manufacturing practice (cGMP) facility and a clinic or between different cGMP facilities requires validated standard operating procedures (SOP). Under National Heart Lung & Blood Institute (NHLBI) sponsorship, the Production Assistance for Cel...

journal_title：Cytotherapy

authors： Whiteside TL,Griffin DL,Stanson J,Gooding W,McKenna D,Sumstad D,Kadidlo D,Gee A,Durett A,Lindblad R,Wood D,Styers D

更新日期：2011-02-01 00:00:00

abstract：BACKGROUND AIMS:Immunomodulatory properties of human umbilical cord-derived mesenchymal stromal cells (UCMSCs) can be differentially modulated by toll-like receptors (TLR) agonists. Here, the therapeutic efficacy of short TLR3 and TLR4 pre-conditioning of UCMSCs was evaluated in a dextran sulfate sodium (DSS)-induced c...

journal_title：Cytotherapy

authors： Fuenzalida P,Kurte M,Fernández-O'ryan C,Ibañez C,Gauthier-Abeliuk M,Vega-Letter AM,Gonzalez P,Irarrázabal C,Quezada N,Figueroa F,Carrión F

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM)-derived mesenchymal stromal cells (MSC) are promising candidate cells for the development of neuroregenerative therapies. We have previously introduced the pro-neural conversion of human MSC to neural stem cell-like cells (m-NSC) by culturing them in suspension culture under serum-free condi...

journal_title：Cytotherapy

authors： Habisch HJ,Fiedler J,Ludolph AC,Storch A,Brenner RE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) suppress T-cell proliferation, especially after activation with inflammatory cytokines. We compared the dynamic action of unprimed and interferon (IFN)-γ plus tumor necrosis factor (TNF)-α-pretreated human bone marrow-derived MSCs on resting or activated T cells. METHOD...

journal_title：Cytotherapy

authors： Cuerquis J,Romieu-Mourez R,François M,Routy JP,Young YK,Zhao J,Eliopoulos N

更新日期：2014-02-01 00:00:00